(Linear) (Six-base) MAPSORT of: KIEE4458.seq  Check: 2566  from: 1  to: 1425



KIEE4458.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1425 
   Size:        281    1144
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1033    1425 
   Size:       1033     392
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     550    1425 
   Size:        550     875
 
AlwNI CAG_nnn'CTG

Cuts at:      0     236     259     369    1425 
   Size:        236      23     110    1056

  Fragments arranged by size: 

                1056     236     110      23
 
ApoI r'AATT_y

Cuts at:      0     508     673    1062    1425 
   Size:        508     165     389     363

  Fragments arranged by size: 

                 508     389     363     165
 
AseI AT'TA_AT

Cuts at:      0    1096    1425 
   Size:       1096     329
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     134     167    1425 
   Size:        134      33    1258

  Fragments arranged by size: 

                1258     134      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     536    1425 
   Size:        536     889
 
BclI T'GATC_A

Cuts at:      0    1166    1425 
   Size:       1166     259
 
BfrBI ATG'CAT

Cuts at:      0    1258    1425 
   Size:       1258     167
 
Bme1580I G_kGCm'C

Cuts at:      0    1016    1425 
   Size:       1016     409
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1018    1425 
   Size:       1018     407
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324    1425 
   Size:        324    1101
 
Bpu10I CC'TnA_GC

Cuts at:      0    1228    1425 
   Size:       1228     197
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      94    1425 
   Size:         94    1331
 
BsaWI w'CCGG_w

Cuts at:      0     156    1425 
   Size:        156    1269
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     994    1425 
   Size:        994     431
 
BsiEI CG_ry'CG

Cuts at:      0      44    1425 
   Size:         44    1381
 
Bsp1286I G_dGCh'C

Cuts at:      0    1016    1425 
   Size:       1016     409
 
BspHI T'CATG_A

Cuts at:      0     488    1425 
   Size:        488     937
 
BsrDI GCAATG_nn'

Cuts at:      0     798     915    1425 
   Size:        798     117     510

  Fragments arranged by size: 

                 798     510     117
 
BsrFI r'CCGG_y

Cuts at:      0      39    1425 
   Size:         39    1386
 
BstYI r'GATC_y

Cuts at:      0     769    1425 
   Size:        769     656
 
Bsu36I CC'TnA_GG

Cuts at:      0     689    1425 
   Size:        689     736
 
BtgI C'CryG_G

Cuts at:      0     418    1425 
   Size:        418    1007
 
BtsI GCAGTG_nn'

Cuts at:      0    1381    1425 
   Size:       1381      44
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1137    1172    1425 
   Size:       1137      35     253

  Fragments arranged by size: 

                1137     253      35
 
EaeI y'GGCC_r

Cuts at:      0      41    1425 
   Size:         41    1384
 
EagI C'GGCC_G

Cuts at:      0      41    1425 
   Size:         41    1384
 
EarI CTCTTCn'nnn_

Cuts at:      0     786     848    1425 
   Size:        786      62     577

  Fragments arranged by size: 

                 786     577      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     324    1425 
   Size:        281      43    1101

  Fragments arranged by size: 

                1101     281      43
 
EcoRI G'AATT_C

Cuts at:      0    1062    1425 
   Size:       1062     363
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      72     286    1425 
   Size:         72     214    1139

  Fragments arranged by size: 

                1139     214      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107    1019    1051    1151    1183    1425 
   Size:         75      32     912      32     100      32     242

  Fragments arranged by size: 

                 912     242     100      75      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     697    1400    1425 
   Size:        697     703      25

  Fragments arranged by size: 

                 703     697      25
 
MslI CAynn'nnrTG

Cuts at:      0     791    1425 
   Size:        791     634
 
MspA1I CmG'CkG

Cuts at:      0     117    1425 
   Size:        117    1308
 
NaeI GCC'GGC

Cuts at:      0      41    1425 
   Size:         41    1384
 
NcoI C'CATG_G

Cuts at:      0     418    1425 
   Size:        418    1007
 
NgoMIV G'CCGG_C

Cuts at:      0      39    1425 
   Size:         39    1386
 
NsiI A_TGCA'T

Cuts at:      0    1260    1425 
   Size:       1260     165
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1008    1425 
   Size:       1008     417
 
PfoI T'CCnGG_A

Cuts at:      0      79     881    1425 
   Size:         79     802     544

  Fragments arranged by size: 

                 802     544      79
 
PsiI TTA'TAA

Cuts at:      0     431    1425 
   Size:        431     994
 
SapI GCTCTTCn'nnn_

Cuts at:      0     786    1425 
   Size:        786     639
 
ScaI AGT'ACT

Cuts at:      0     859    1425 
   Size:        859     566
 
StyI C'CwwG_G

Cuts at:      0     418    1425 
   Size:        418    1007
 
TatI w'GTAC_w

Cuts at:      0     857     864     993    1425 
   Size:        857       7     129     432

  Fragments arranged by size: 

                 857     432     129       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      13      45    1425 
   Size:         13      32    1380

  Fragments arranged by size: 

                1380      32      13
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     889    1425 
   Size:        889     536
 
XmnI GAAnn'nnTTC

Cuts at:      0    1066    1425 
   Size:       1066     359
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI    AlwNI     ApoI     AseI     BaeI     BbsI
     BclI    BfrBI Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaWI
    BseRI    BsiEI Bsp1286I    BspHI    BsrDI    BsrFI    BstYI   Bsu36I
     BtgI     BtsI    CspCI     EaeI     EagI     EarI  Eco57MI    EcoRI
     FalI    Hin4I  HindIII     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NsiI    PflMI     PfoI     PsiI     SapI     ScaI     StyI     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI     DraI   DraIII
     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NarI     NdeI     NheI     NotI     NruI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     ZraI