################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccII 292 CGCG CG^CG AlwI 423 GATCC GGATC(4/5) AspHI 573 GAGCAC G[AT]GC[AT]^C AvaII 435 GGTCC G^G[AT]CC BalI 411 TGGCCA TGG^CCA BbeI 637 GGCGCC GGCGC^C BbsI 588 GAAGAC GAAGAC(2/6) BbvI 351 GCAGC GCAGC(8/12) BcnI 627 CCCGG CC^[GC]GG BglI 404 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 570 GCTGAGC GC^T[ACGT]AGC Bpu1102I 570 GCTGAGC GC^T[ACGT]AGC BpuAI 588 GAAGAC GAAGAC(2/6) BsiHKAI 573 GAGCAC G[AT]GC[AT]^C BsmAI 53 GTCTC GTCTC(1/5) BsmFI 461 GGGAC GGGAC(10/14) BspHI 386 TCATGA T^CATGA BstAPI 215 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 292 CGCG CG^CG BstYI 422 AGATCC [GA]^GATC[CT] CelII 570 GCTGAGC GC^T[ACGT]AGC CfoI 638 GCGC GCG^C Cfr13I 435 GGTCC G^G[ACGT]CC DraII 434 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 445 GAAGAG CTCTTC(1/4) EcoO109I 434 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 414 CCATGG C^C[AT][AT]GG Fnu4HI 351 GCAGC GC^[ACGT]GC HaeII 637 GGCGCC [GA]GCGC^[CT] HgaI 133 GACGC GACGC(5/10) HhaI 638 GCGC GCG^C HinP1I 638 GCGC G^CGC HinfI 463 GACTC G^A[ACGT]TC ItaI 351 GCAGC GC^[ACGT]GC KasI 637 GGCGCC G^GCGCC Ksp632I 445 GAAGAG CTCTTC(1/4) MaeIII 94 GTCAC ^GT[ACGT]AC MflI 422 AGATCC [GA]^GATC[CT] MluNI 411 TGGCCA TGG^CCA MscI 411 TGGCCA TGG^CCA MseI 263 TTAA T^TAA MvnI 292 CGCG CG^CG NarI 637 GGCGCC GG^CGCC NciI 627 CCCGG CC^[GC]GG NcoI 414 CCATGG C^CATGG PleI 463 GACTC GAGTC(4/5) PpuMI 434 AGGTCCT [GA]G^G[AT]CC[CT] PstI 430 CTGCAG CTGCA^G RcaI 386 TCATGA T^CATGA SapI 445 GAAGAGC GCTCTTC(1/4) Sau96I 435 GGTCC G^G[ACGT]CC SfcI 430 CTGCAG C^T[GA][CT]AG SfoI 637 GGCGCC GGC^GCC StyI 414 CCATGG C^C[AT][AT]GG Tru9I 263 TTAA T^TAA TseI 351 GCAGC G^C[AT]GC Tsp45I 94 GTCAC ^GT[GC]AC TspRI 170 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 405 CCACCGTGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 422 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 133 GACGCC G[GA]^CG[CT]C 637 GGCGCC AfaI 100 GTAC GT^AC 321 GTAC AluI 272 AGCT AG^CT 569 AGCT AlwNI 98 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 565 CAGAAGCTG BanII 46 GGGCTC G[GA]GC[CT]^C 448 GAGCCC BpmI 241 CTCCAG CTGGAG(16/14) 274 CTGGAG BsaHI 133 GACGCC G[GA]^CG[CT]C 637 GGCGCC BsgI 147 CTGCAC GTGCAG(16/14) 349 GTGCAG BspMI 102 ACCTGC ACCTGC(4/8) 432 GCAGGT BsrBI 277 GAGCGG CCGCTC(-3/-3) 457 GAGCGG BsrI 197 ACTGG ACTGG(1/-1) 243 CCAGT BstF5I 360 GGATG GGATG(2/0) 630 GGATG Cac8I 136 GCCCGC GC[ACGT]^[ACGT]GC 140 GCCTGC DpnII 258 GATC ^GATC 423 GATC EaeI 411 TGGCCA [CT]^GGCC[GA] 473 TGGCCG FauI 137 CCCGC CCCGC(4/6) 459 GCGGG FokI 360 GGATG GGATG(9/13) 630 GGATG HaeIII 412 GGCC GG^CC 474 GGCC HapII 355 CCGG C^CGG 628 CCGG Hin1I 133 GACGCC G[GA]^CG[CT]C 637 GGCGCC HpaII 355 CCGG C^CGG 628 CCGG HphI 333 GGTGA GGTGA(8/7) 616 GGTGA MaeII 116 ACGT A^CGT 347 ACGT MboI 258 GATC ^GATC 423 GATC MslI 4 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 519 CATCTTGGTG MspI 355 CCGG C^CGG 628 CCGG NdeII 258 GATC ^GATC 423 GATC RsaI 100 GTAC GT^AC 321 GTAC Sau3AI 258 GATC ^GATC 423 GATC SfaNI 10 GATGC GCATC(5/9) 336 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI BanI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI DdeI DsaI EcoRII MboII MnlI MvaI MwoI NlaIII NlaIV ScrFI