(Circular) (Six-base) MAPSORT of: pFN21AE5214.seq Check: 1227 from: 1 to: 5350 pFN21AE5214 5350 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3160 278 Size: 53 83 186 936 1624 2468 Fragments arranged by size: 2468 1624 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2693 1937 Size: 756 4594 Acc65I G'GTAC_C Cuts at: 2671 2671 Size: 5350 AclI AA'CG_TT Cuts at: 1563 3477 3850 1563 Size: 1914 373 3063 Fragments arranged by size: 3063 1914 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2023 2294 2612 3413 4797 5065 1632 Size: 391 271 318 801 1384 268 1917 Fragments arranged by size: 1917 1384 801 391 318 271 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5153 AflIII A'CryG_T Cuts at: 1203 4249 5226 1203 Size: 3046 977 1327 Fragments arranged by size: 3046 1327 977 AgeI A'CCGG_T Cuts at: 4181 4181 Size: 5350 AhdI GACnn_n'nnGTC Cuts at: 4079 4079 Size: 5350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2031 1184 Size: 847 4503 AlwNI CAG_nnn'CTG Cuts at: 2108 2575 3093 4665 2108 Size: 467 518 1572 2793 Fragments arranged by size: 2793 1572 518 467 ApaLI G'TGCA_C Cuts at: 3407 4563 3407 Size: 1156 4194 ApoI r'AATT_y Cuts at: 1493 2659 2885 3037 5146 1493 Size: 1166 226 152 2109 1697 Fragments arranged by size: 2109 1697 1166 226 152 AseI AT'TA_AT Cuts at: 160 1032 3904 160 Size: 872 2872 1606 Fragments arranged by size: 2872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 AvaI C'yCGr_G Cuts at: 1870 1943 2675 1870 Size: 73 732 4545 Fragments arranged by size: 4545 732 73 BamHI G'GATC_C Cuts at: 2680 3047 2680 Size: 367 4983 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2492 2642 2671 4126 Size: 325 217 627 287 418 150 29 1455 Cuts at: 4126 618 Size: 1842 Fragments arranged by size: 1842 1455 627 418 325 287 217 150 29 BanII G_rGCy'C Cuts at: 1391 2055 2457 2669 1391 Size: 664 402 212 4072 Fragments arranged by size: 4072 664 402 212 BbeI G_GCGC'C Cuts at: 2646 2646 Size: 5350 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2600 928 Size: 971 701 3678 Fragments arranged by size: 3678 971 701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3541 3575 1903 Size: 34 50 34 1520 34 3678 Fragments arranged by size: 3678 1520 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3242 4452 3242 Size: 1210 4140 BclI T'GATC_A Cuts at: 1548 1548 Size: 5350 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2415 3961 136 Size: 107 122 71 631 605 743 1546 1525 Fragments arranged by size: 1546 1525 743 631 605 122 107 71 BglII A'GATC_T Cuts at: 5345 5345 Size: 5350 BlpI GC'TnA_GC Cuts at: 2576 2767 2576 Size: 191 5159 Bme1580I G_kGCm'C Cuts at: 1790 3411 4567 1790 Size: 1621 1156 2573 Fragments arranged by size: 2573 1621 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3081 4039 442 Size: 416 53 83 399 24 1664 958 1753 Fragments arranged by size: 1753 1664 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5350 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 5350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2300 4010 1239 Size: 992 69 1710 2579 Fragments arranged by size: 2579 1710 992 69 Bpu10I CC'TnA_GC Cuts at: 2460 2460 Size: 5350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3475 4340 4638 3475 Size: 865 298 4187 Fragments arranged by size: 4187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4013 882 Size: 617 2514 2219 Fragments arranged by size: 2514 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3141 493 Size: 711 1937 2702 Fragments arranged by size: 2702 1937 711 BsaBI GATnn'nnATC Cuts at: 5344 5344 Size: 5350 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 2643 3157 3539 Size: 53 83 186 936 606 504 514 382 Cuts at: 3539 4174 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 606 514 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3783 4181 4455 4602 5013 3783 Size: 398 274 147 411 4120 Fragments arranged by size: 4120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5125 Fragments arranged by size: 5125 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2569 2615 2618 1186 Size: 1063 320 46 3 3918 Fragments arranged by size: 3918 1063 320 46 3 BseYI C'CCAG_C Cuts at: 2318 4553 5049 2318 Size: 2235 496 2619 Fragments arranged by size: 2619 2235 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2137 2375 1163 Size: 573 401 238 4138 Fragments arranged by size: 4138 573 401 238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2800 3561 4589 1540 Size: 275 186 799 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2582 3411 3496 4567 2582 Size: 829 85 1071 3365 Fragments arranged by size: 3365 1071 829 85 BsmI GAATG_Cn' Cuts at: 2861 2954 2861 Size: 93 5257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2457 2582 2669 3411 3496 4567 Size: 399 265 402 125 87 742 85 1071 Cuts at: 4567 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 402 399 265 125 87 85 BspEI T'CCGG_A Cuts at: 5013 5013 Size: 5350 BspHI T'CATG_A Cuts at: 2391 3239 4969 2391 Size: 848 1730 2772 Fragments arranged by size: 2772 1730 848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2116 2428 2705 844 Size: 1272 312 277 3489 Fragments arranged by size: 3489 1272 312 277 BsrBI CCG'CTC Cuts at: 1394 1823 2284 2464 3237 1394 Size: 429 461 180 773 3507 Fragments arranged by size: 3507 773 461 429 180 BsrDI GCAATG_nn' Cuts at: 65 1248 3845 4019 65 Size: 1183 2597 174 1396 Fragments arranged by size: 2597 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3994 4181 1700 Size: 110 2184 187 2869 Fragments arranged by size: 2869 2184 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5350 BssSI C'ACGA_G Cuts at: 3410 4422 3410 Size: 1012 4338 BstAPI GCAn_nnn'nTGC Cuts at: 794 2226 794 Size: 1432 3918 BstBI TT'CG_AA Cuts at: 4154 4154 Size: 5350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5350 BstYI r'GATC_y Cuts at: 1972 2427 2680 3047 3432 3449 4901 4987 4999 Size: 455 253 367 385 17 1452 86 12 Cuts at: 4999 5345 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 455 385 367 346 253 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2413 2419 2472 513 Size: 552 1348 6 53 3391 Fragments arranged by size: 3391 1348 552 53 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4611 Fragments arranged by size: 4611 683 56 BtsI GCAGTG_nn' Cuts at: 2866 3660 3680 2866 Size: 794 20 4536 Fragments arranged by size: 4536 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5315 DraI TTT'AAA Cuts at: 2654 2654 Size: 5350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4357 809 Size: 507 3041 1802 Fragments arranged by size: 3041 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2416 2478 2797 Size: 54 1425 50 275 18 586 62 319 Cuts at: 2797 3686 5315 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 586 319 275 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2797 1537 Size: 275 985 4090 Fragments arranged by size: 4090 985 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2445 3280 1353 Size: 1092 835 3423 Fragments arranged by size: 3423 1092 835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3937 4311 4457 387 Size: 1064 138 2348 374 146 1280 Fragments arranged by size: 2348 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2231 2294 2300 2612 3413 4010 Size: 393 391 208 63 6 312 801 597 Cuts at: 4010 4797 5065 1239 Size: 787 268 1524 Fragments arranged by size: 1524 801 787 597 393 391 312 268 208 63 6 EcoO109I rG'GnC_Cy Cuts at: 2440 2440 Size: 5350 EcoRI G'AATT_C Cuts at: 2659 3037 2659 Size: 378 4972 FspI TGC'GCA Cuts at: 3856 3856 Size: 5350 HaeII r_GCGC'y Cuts at: 1678 2646 4497 1678 Size: 968 1851 2531 Fragments arranged by size: 2531 1851 968 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3991 4023 4065 4097 1373 Size: 32 135 32 2419 32 42 32 2626 Fragments arranged by size: 2626 2419 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2694 2940 669 Size: 988 281 756 246 3079 Fragments arranged by size: 3079 988 756 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5350 HpaI GTT'AAC Cuts at: 2940 2940 Size: 5350 KasI G'GCGC_C Cuts at: 2642 2642 Size: 5350 KpnI G_GTAC'C Cuts at: 2675 2675 Size: 5350 MfeI C'AATT_G Cuts at: 2949 2949 Size: 5350 MluI A'CGCG_T Cuts at: 5226 5226 Size: 5350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4464 4648 1305 Size: 3159 184 2007 Fragments arranged by size: 3159 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2418 10 Size: 54 1425 343 586 2942 Fragments arranged by size: 2942 1425 586 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2013 2528 3308 3667 3826 518 Size: 648 847 515 780 359 159 2042 Fragments arranged by size: 2042 847 780 648 515 359 159 MspA1I CmG'CkG Cuts at: 1606 2766 3443 4591 4836 1606 Size: 1160 677 1148 245 2120 Fragments arranged by size: 2120 1160 1148 677 245 NarI GG'CG_CC Cuts at: 2643 2643 Size: 5350 NcoI C'CATG_G Cuts at: 513 1065 2419 513 Size: 552 1354 3444 Fragments arranged by size: 3444 1354 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5350 NotI GC'GGCC_GC Cuts at: 2797 2797 Size: 5350 NspI r_CATG'y Cuts at: 1132 1229 2708 4253 1132 Size: 97 1479 1545 2229 Fragments arranged by size: 2229 1545 1479 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5350 PciI A'CATG_T Cuts at: 4249 4249 Size: 5350 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5350 PmeI GTTT'AAAC Cuts at: 2654 2654 Size: 5350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 2257 3419 3451 4932 4964 2225 Size: 32 1162 32 1481 32 2611 Fragments arranged by size: 2611 1481 1162 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2440 2440 Size: 5350 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5350 PsiI TTA'TAA Cuts at: 2920 2920 Size: 5350 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2192 2224 2192 Size: 32 5318 PstI C_TGCA'G Cuts at: 830 2439 2702 830 Size: 1609 263 3478 Fragments arranged by size: 3478 1609 263 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5350 SalI G'TCGA_C Cuts at: 1936 2692 1936 Size: 756 4594 SapI GCTCTTCn'nnn_ Cuts at: 2445 2445 Size: 5350 SbfI CC_TGCA'GG Cuts at: 2702 2702 Size: 5350 ScaI AGT'ACT Cuts at: 3598 5310 3598 Size: 1712 3638 SfcI C'TryA_G Cuts at: 826 1045 2435 2698 3833 4514 4705 826 Size: 219 1390 263 1135 681 191 1471 Fragments arranged by size: 1471 1390 1135 681 263 219 191 SfoI GGC'GCC Cuts at: 2644 2644 Size: 5350 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5350 SmaI CCC'GGG Cuts at: 1872 2677 1872 Size: 805 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3454 4355 4617 820 Size: 197 926 1511 901 262 1553 Fragments arranged by size: 1553 1511 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3141 493 Size: 2648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5350 SphI G_CATG'C Cuts at: 1132 2708 1132 Size: 1576 3774 SspI AAT'ATT Cuts at: 5 52 3274 5 Size: 47 3222 2081 Fragments arranged by size: 3222 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5350 StyI C'CwwG_G Cuts at: 513 1065 2419 2646 2789 513 Size: 552 1354 227 143 3074 Fragments arranged by size: 3074 1354 552 227 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2480 2612 3377 3394 3547 3732 4177 Size: 1255 900 132 765 17 153 185 445 Cuts at: 4177 325 Size: 1498 Fragments arranged by size: 1498 1255 900 765 445 185 153 132 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3596 5308 96 Size: 274 80 33 51 1443 1619 1712 138 Fragments arranged by size: 1712 1619 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5310 5342 5310 Size: 32 5318 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5350 XbaI T'CTAG_A Cuts at: 2686 2686 Size: 5350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2417 2417 Size: 5350 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5350 XmaI C'CCGG_G Cuts at: 1870 2675 1870 Size: 805 4545 XmnI GAAnn'nnTTC Cuts at: 3479 3479 Size: 5350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3158 276 Size: 53 83 186 936 1624 2468 Fragments arranged by size: 2468 1624 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI