(Linear) (Six-base) MAPSORT of: KIEE5214.seq Check: 4270 from: 1 to: 645 KIEE5214.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 18 289 607 645 Size: 18 271 318 38 Fragments arranged by size: 318 271 38 18 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 26 645 Size: 26 619 AlwNI CAG_nnn'CTG Cuts at: 0 103 570 645 Size: 103 467 75 Fragments arranged by size: 467 103 75 BanI G'GyrC_C Cuts at: 0 69 487 637 645 Size: 69 418 150 8 Fragments arranged by size: 418 150 69 8 BanII G_rGCy'C Cuts at: 0 50 452 645 Size: 50 402 193 Fragments arranged by size: 402 193 50 BbeI G_GCGC'C Cuts at: 0 641 645 Size: 641 4 BbsI GAAGACnn'nnnn_ Cuts at: 0 595 645 Size: 595 50 BglI GCCn_nnn'nGGC Cuts at: 0 410 645 Size: 410 235 BlpI GC'TnA_GC Cuts at: 0 571 645 Size: 571 74 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 480 645 Size: 480 165 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 295 645 Size: 226 69 350 Fragments arranged by size: 350 226 69 Bpu10I CC'TnA_GC Cuts at: 0 455 645 Size: 455 190 BsaHI Gr'CG_yC Cuts at: 0 134 638 645 Size: 134 504 7 Fragments arranged by size: 504 134 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 244 564 610 613 645 Size: 244 320 46 3 32 Fragments arranged by size: 320 244 46 32 3 BseYI C'CCAG_C Cuts at: 0 313 645 Size: 313 332 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 132 370 645 Size: 132 238 275 Fragments arranged by size: 275 238 132 BsiHKAI G_wGCw'C Cuts at: 0 577 645 Size: 577 68 Bsp1286I G_dGCh'C Cuts at: 0 50 452 577 645 Size: 50 402 125 68 Fragments arranged by size: 402 125 68 50 BspHI T'CATG_A Cuts at: 0 386 645 Size: 386 259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 111 423 645 Size: 111 312 222 Fragments arranged by size: 312 222 111 BsrBI CCG'CTC Cuts at: 0 279 459 645 Size: 279 180 186 Fragments arranged by size: 279 186 180 BstAPI GCAn_nnn'nTGC Cuts at: 0 221 645 Size: 221 424 BstYI r'GATC_y Cuts at: 0 422 645 Size: 422 223 BtgI C'CryG_G Cuts at: 0 408 414 467 645 Size: 408 6 53 178 Fragments arranged by size: 408 178 53 6 EaeI y'GGCC_r Cuts at: 0 411 473 645 Size: 411 62 172 Fragments arranged by size: 411 172 62 EarI CTCTTCn'nnn_ Cuts at: 0 440 645 Size: 440 205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 18 226 289 295 607 645 Size: 18 208 63 6 312 38 Fragments arranged by size: 312 208 63 38 18 6 EcoO109I rG'GnC_Cy Cuts at: 0 435 645 Size: 435 210 HaeII r_GCGC'y Cuts at: 0 641 645 Size: 641 4 KasI G'GCGC_C Cuts at: 0 637 645 Size: 637 8 MscI TGG'CCA Cuts at: 0 413 645 Size: 413 232 MslI CAynn'nnrTG Cuts at: 0 8 523 645 Size: 8 515 122 Fragments arranged by size: 515 122 8 NarI GG'CG_CC Cuts at: 0 638 645 Size: 638 7 NcoI C'CATG_G Cuts at: 0 414 645 Size: 414 231 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 220 252 645 Size: 220 32 393 Fragments arranged by size: 393 220 32 PpuMI rG'GwC_Cy Cuts at: 0 435 645 Size: 435 210 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 187 219 645 Size: 187 32 426 Fragments arranged by size: 426 187 32 PstI C_TGCA'G Cuts at: 0 434 645 Size: 434 211 SapI GCTCTTCn'nnn_ Cuts at: 0 440 645 Size: 440 205 SfcI C'TryA_G Cuts at: 0 430 645 Size: 430 215 SfoI GGC'GCC Cuts at: 0 639 645 Size: 639 6 StyI C'CwwG_G Cuts at: 0 414 645 Size: 414 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 475 607 645 Size: 475 132 38 Fragments arranged by size: 475 132 38 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 412 645 Size: 412 233 Enzymes that do cut: AcuI AlfI AlwNI BanI BanII BbeI BbsI BglI BlpI BplI BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BstAPI BstYI BtgI EaeI EarI Eco57MI EcoO109I HaeII KasI MscI MslI NarI NcoI PpiI PpuMI PsrI PstI SapI SfcI SfoI StyI TaqII XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI