Restriction map for
pFN21AE1550
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccII AcyI AflII
AflIII AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbeI BbrPI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MunI MvnI NarI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1536 GGTACC G^GTACC
AccIII 2103 TCCGGA T^CCGGA
AgeI 2069 ACCGGT A^CCGGT
Alw44I 1269 GTGCAC G^TGCAC
ApaI 353 GGGCCC GGGCC^C
ApaLI 1269 GTGCAC G^TGCAC
Asp718I 1536 GGTACC G^GTACC
AvaI 134 CCCGAG C^[CT]CG[GA]G
BalI 956 TGGCCA TGG^CCA
BamHI 1419 GGATCC G^GATCC
BanI 1536 GGTACC G^G[CT][GA]CC
BcnI 1278 CCGGG CC^[GC]GG
BglI 1624 GCCGATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 575 AGATCT A^GATCT
BsaI 2045 GAGACC GGTCTC(1/5)
BseAI 2103 TCCGGA T^CCGGA
BsgI 1322 GTGCAG GTGCAG(16/14)
BsmBI 61 GAGACG CGTCTC(1/5)
BsoBI 134 CCCGAG C^[CT]CG[GA]G
BspEI 2103 TCCGGA T^CCGGA
BspMI 177 GCAGGT ACCTGC(4/8)
BsrDI 1233 CATTGC GCAATG(2/0)
BssSI 482 CTCGTG CACGAG(-5/-1)
BstAPI 1701 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 163 CCAGGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1448 GACATCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1360 GATATC GAT^ATC
KpnI 1536 GGTACC GGTAC^C
MluNI 956 TGGCCA TGG^CCA
MroI 2103 TCCGGA T^CCGGA
MscI 956 TGGCCA TGG^CCA
NaeI 2435 GCCGGC GCC^GGC
NciI 1278 CCGGG CC^[GC]GG
NgoMIV 2435 GCCGGC G^CCGGC
NheI 370 GCTAGC G^CTAGC
NspI 1256 GCATGT [GA]CATG^[CT]
PinAI 2069 ACCGGT A^CCGGT
PpuMI 1964 AGGACCC [GA]G^G[AT]CC[CT]
SacI 910 GAGCTC GAGCT^C
SexAI 227 ACCTGGT A^CC[AT]GGT
SmlI 2475 CTCAAG C^T[CT][GA]AG
StuI 1998 AGGCCT AGG^CCT
TaqI 1613 TCGA T^CGA
TthHB8I 1613 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AclI 287 AACGTT AA^CGTT
361 AACGTT
AcsI 625 AAATTC [GA]^AATT[CT]
640 AAATTT
AocI 303 CCTGAGG CC^T[ACGT]AGG
1774 CCTAAGG
ApoI 625 AAATTC [GA]^AATT[CT]
640 AAATTT
BanII 353 GGGCCC G[GA]GC[CT]^C
910 GAGCTC
BbsI 318 GAAGAC GAAGAC(2/6)
1734 GAAGAC
BcgI 1614 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1995 CGAAGGCCTTGC
BpuAI 318 GAAGAC GAAGAC(2/6)
1734 GAAGAC
Bsu36I 303 CCTGAGG CC^T[ACGT]AGG
1774 CCTAAGG
EaeI 956 TGGCCA [CT]^GGCC[GA]
2437 CGGCCA
Eco81I 303 CCTGAGG CC^T[ACGT]AGG
1774 CCTAAGG
EcoNI 303 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
798 CCTCTCCAAGG
FauI 1191 GCGGG CCCGC(4/6)
2193 GCGGG
HincII 616 GTTGAC GT[CT]^[GA]AC
790 GTTGAC
HindII 616 GTTGAC GT[CT]^[GA]AC
790 GTTGAC
MseI 650 TTAA T^TAA
2081 TTAA
MslI 867 CATACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
888 CATCAGAGTG
Psp1406I 287 AACGTT AA^CGTT
361 AACGTT
ScaI 1557 AGTACT AGT^ACT
1861 AGTACT
Tru9I 650 TTAA T^TAA
2081 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BbvCI BbvI BciVI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII