(Circular) (Six-base) MAPSORT of: pFN21AE1550.seq Check: 7108 from: 1 to: 7246 pFN21AE1550 7246 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5056 278 Size: 53 83 186 936 3520 2468 Fragments arranged by size: 3520 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4589 1937 Size: 2652 4594 Acc65I G'GTAC_C Cuts at: 3541 4567 3541 Size: 1026 6220 AclI AA'CG_TT Cuts at: 1563 2293 2367 5373 5746 1563 Size: 730 74 3006 373 3063 Fragments arranged by size: 3063 3006 730 373 74 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2739 3233 3513 4286 5309 6693 6961 Size: 710 397 494 280 773 1023 1384 268 Cuts at: 6961 1632 Size: 1917 Fragments arranged by size: 1917 1384 1023 773 710 494 397 280 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7049 AflIII A'CryG_T Cuts at: 1203 6145 7122 1203 Size: 4942 977 1327 Fragments arranged by size: 4942 1327 977 AgeI A'CCGG_T Cuts at: 4074 6077 4074 Size: 2003 5243 AhdI GACnn_n'nnGTC Cuts at: 5975 5975 Size: 7246 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2205 1184 Size: 1021 6225 AlwNI CAG_nnn'CTG Cuts at: 2128 2308 2503 2524 2768 3712 3812 4989 6561 Size: 180 195 21 244 944 100 1177 1572 Cuts at: 6561 2128 Size: 2813 Fragments arranged by size: 2813 1572 1177 944 244 195 180 100 21 ApaI G_GGCC'C Cuts at: 2362 2362 Size: 7246 ApaLI G'TGCA_C Cuts at: 3274 5303 6459 3274 Size: 2029 1156 4061 Fragments arranged by size: 4061 2029 1156 ApoI r'AATT_y Cuts at: 1493 2630 2645 4555 4781 4933 7042 1493 Size: 1137 15 1910 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 1137 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 5800 160 Size: 872 4768 1606 Fragments arranged by size: 4768 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7246 AvaI C'yCGr_G Cuts at: 1870 1943 2139 4571 1870 Size: 73 196 2432 4545 Fragments arranged by size: 4545 2432 196 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4136 4169 4136 Size: 33 7213 BamHI G'GATC_C Cuts at: 3424 4576 4943 3424 Size: 1152 367 5727 Fragments arranged by size: 5727 1152 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3541 4567 6022 618 Size: 325 217 627 1754 1026 1455 1842 Fragments arranged by size: 1842 1754 1455 1026 627 325 217 BanII G_rGCy'C Cuts at: 1391 2362 2919 4565 1391 Size: 971 557 1646 4072 Fragments arranged by size: 4072 1646 971 557 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2330 3746 928 Size: 971 431 1416 4428 Fragments arranged by size: 4428 1416 971 431 BbvCI CC'TCA_GC Cuts at: 2730 3005 4233 2730 Size: 275 1228 5743 Fragments arranged by size: 5743 1228 275 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3608 3642 3989 4023 5437 5471 1903 Size: 34 1671 34 347 34 1414 34 3678 Fragments arranged by size: 3678 1671 1414 347 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2218 3981 4137 5138 6348 2218 Size: 1763 156 1001 1210 3116 Fragments arranged by size: 3116 1763 1210 1001 156 BclI T'GATC_A Cuts at: 1548 1548 Size: 7246 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3635 5857 136 Size: 107 122 71 631 605 1963 2222 1525 Fragments arranged by size: 2222 1963 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2580 7241 2580 Size: 4661 2585 BlpI GC'TnA_GC Cuts at: 4663 4663 Size: 7246 Bme1580I G_kGCm'C Cuts at: 1790 2362 3278 5307 6463 1790 Size: 572 916 2029 1156 2573 Fragments arranged by size: 2573 2029 1156 916 572 BmgBI CAC'GTC Cuts at: 1318 4214 1318 Size: 2896 4350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4977 5935 442 Size: 416 53 83 399 24 3560 958 1753 Fragments arranged by size: 3560 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2379 1056 Size: 1323 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3058 3058 Size: 7246 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 2956 3033 3569 3662 4295 4470 5906 Size: 1016 701 77 536 93 633 175 1436 Cuts at: 5906 1239 Size: 2579 Fragments arranged by size: 2579 1436 1016 701 633 536 175 93 77 Bpu10I CC'TnA_GC Cuts at: 2730 2918 3005 4233 4239 2730 Size: 188 87 1228 6 5737 Fragments arranged by size: 5737 1228 188 87 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4465 5371 6236 6534 4465 Size: 906 865 298 5177 Fragments arranged by size: 5177 906 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4044 5909 882 Size: 617 2545 1865 2219 Fragments arranged by size: 2545 2219 1865 617 BsaAI yAC'GTr Cuts at: 493 1204 5037 493 Size: 711 3833 2702 Fragments arranged by size: 3833 2702 711 BsaBI GATnn'nnATC Cuts at: 7240 7240 Size: 7246 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5053 5435 6070 275 Size: 53 83 186 936 3520 382 635 1451 Fragments arranged by size: 3520 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4108 4140 4185 5679 6077 6351 6498 6909 Size: 34 32 45 1494 398 274 147 411 Cuts at: 6909 4074 Size: 4411 Fragments arranged by size: 4411 1494 411 398 274 147 45 34 32 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2789 2819 2906 2936 1178 Size: 30 165 30 1386 30 87 30 5488 Fragments arranged by size: 5488 1386 165 87 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3050 3092 3095 3104 3107 3110 3446 3527 Size: 1864 42 3 9 3 3 336 81 Cuts at: 3527 3530 3533 3536 3539 1186 Size: 3 3 3 3 4893 Fragments arranged by size: 4893 1864 336 81 42 9 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2379 3611 3657 3891 3973 4370 6449 6945 2379 Size: 1232 46 234 82 397 2079 496 2680 Fragments arranged by size: 2680 2079 1232 496 397 234 82 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3348 1163 Size: 573 1612 5061 Fragments arranged by size: 5061 1612 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4696 5457 6485 1540 Size: 275 186 2695 761 1028 2301 Fragments arranged by size: 2695 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2349 2919 3278 4507 5307 5392 6463 2349 Size: 570 359 1229 800 85 1071 3132 Fragments arranged by size: 3132 1229 1071 800 570 359 85 BsmI GAATG_Cn' Cuts at: 4757 4850 4757 Size: 93 7153 BsmBI CGTCTCn'nnnn_ Cuts at: 2060 2060 Size: 7246 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2349 2362 2919 3278 4507 4565 5307 Size: 399 559 13 557 359 1229 58 742 Cuts at: 5307 5392 6463 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1229 1071 742 559 557 399 359 85 58 13 BspEI T'CCGG_A Cuts at: 4108 6909 4108 Size: 2801 4445 BspHI T'CATG_A Cuts at: 5135 6865 5135 Size: 1730 5516 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2173 4601 844 Size: 1329 2428 3489 Fragments arranged by size: 3489 2428 1329 BsrBI CCG'CTC Cuts at: 1394 1823 5133 1394 Size: 429 3310 3507 Fragments arranged by size: 3507 3310 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3237 5741 5915 65 Size: 1183 1989 2504 174 1396 Fragments arranged by size: 2504 1989 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3675 4074 4440 5890 6077 1700 Size: 110 1865 399 366 1450 187 2869 Fragments arranged by size: 2869 1865 1450 399 366 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7246 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7246 BssSI C'ACGA_G Cuts at: 2487 5306 6318 2487 Size: 2819 1012 3415 Fragments arranged by size: 3415 2819 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3712 794 Size: 2918 4328 BstBI TT'CG_AA Cuts at: 6050 6050 Size: 7246 BstEII G'GTnAC_C Cuts at: 1184 2072 2735 3287 1184 Size: 888 663 552 5143 Fragments arranged by size: 5143 888 663 552 BstXI CCAn_nnnn'nTGG Cuts at: 2175 2175 Size: 7246 BstYI r'GATC_y Cuts at: 1972 2511 2580 3220 3424 4576 4943 5328 5345 Size: 539 69 640 204 1152 367 385 17 Cuts at: 5345 6797 6883 6895 7241 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1152 640 539 385 367 346 204 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 2309 3780 2309 Size: 1471 5775 BtgI C'CryG_G Cuts at: 513 1065 2004 2452 3904 3922 4302 513 Size: 552 939 448 1452 18 380 3457 Fragments arranged by size: 3457 1452 939 552 448 380 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6507 Fragments arranged by size: 6507 683 56 BtsI GCAGTG_nn' Cuts at: 3533 3880 4762 5556 5576 3533 Size: 347 882 794 20 5203 Fragments arranged by size: 5203 882 794 347 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7246 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3805 3840 529 Size: 35 3241 35 3935 Fragments arranged by size: 3935 3241 35 35 DraI TTT'AAA Cuts at: 4550 4550 Size: 7246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3459 6253 809 Size: 507 2143 2794 1802 Fragments arranged by size: 2794 2143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2961 4442 4693 Size: 54 1425 50 275 18 1131 1481 251 Cuts at: 4693 5582 7211 8 Size: 889 1629 43 Fragments arranged by size: 1629 1481 1425 1131 889 275 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4693 1537 Size: 275 2881 4090 Fragments arranged by size: 4090 2881 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2832 3081 3149 3879 4036 4262 5176 1353 Size: 1479 249 68 730 157 226 914 3423 Fragments arranged by size: 3423 1479 914 730 249 226 157 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2519 3161 3694 5833 6207 6353 Size: 1064 138 930 642 533 2139 374 146 Cuts at: 6353 387 Size: 1280 Fragments arranged by size: 2139 1280 1064 930 642 533 374 146 138 EcoICRI GAG'CTC Cuts at: 2917 2917 Size: 7246 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2342 2739 2956 3033 3233 3513 Size: 393 623 87 397 217 77 200 280 Cuts at: 3513 3569 3662 4286 4295 4470 5309 5906 6693 Size: 56 93 624 9 175 839 597 787 Cuts at: 6693 6961 1239 Size: 268 1524 Fragments arranged by size: 1524 839 787 624 623 597 397 393 280 268 217 200 175 93 87 77 56 9 EcoNI CCTnn'n_nnAGG Cuts at: 2312 2807 2312 Size: 495 6751 EcoO109I rG'GnC_Cy Cuts at: 2305 2358 2359 3637 3970 2305 Size: 53 1 1278 333 5581 Fragments arranged by size: 5581 1278 333 53 1 EcoRI G'AATT_C Cuts at: 4555 4933 4555 Size: 378 6868 EcoRV GAT'ATC Cuts at: 3367 3367 Size: 7246 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3622 3622 Size: 7246 FspI TGC'GCA Cuts at: 5752 5752 Size: 7246 HaeII r_GCGC'y Cuts at: 1678 6393 1678 Size: 4715 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2789 2821 2906 Size: 32 135 32 809 32 376 32 85 Cuts at: 2906 2938 3389 3421 3737 3739 3769 3771 3833 Size: 32 451 32 316 2 30 2 62 Cuts at: 3833 3865 5887 5919 5961 5993 1373 Size: 32 2022 32 42 32 2626 Fragments arranged by size: 2626 2022 809 451 376 316 135 85 62 42 32 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2623 2797 4590 4836 669 Size: 988 281 685 174 1793 246 3079 Fragments arranged by size: 3079 1793 988 685 281 246 174 HindIII A'AGCT_T Cuts at: 748 748 Size: 7246 HpaI GTT'AAC Cuts at: 4836 4836 Size: 7246 KpnI G_GTAC'C Cuts at: 3545 4571 3545 Size: 1026 6220 MfeI C'AATT_G Cuts at: 4845 4845 Size: 7246 MluI A'CGCG_T Cuts at: 7122 7122 Size: 7246 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6360 6544 1305 Size: 5055 184 2007 Fragments arranged by size: 5055 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2963 10 Size: 54 1425 343 1131 4293 Fragments arranged by size: 4293 1425 1131 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 2897 5204 5563 5722 518 Size: 648 1710 21 2307 359 159 2042 Fragments arranged by size: 2307 2042 1710 648 359 159 21 MspA1I CmG'CkG Cuts at: 1606 2524 3050 3196 3661 3712 4348 4448 4662 Size: 918 526 146 465 51 636 100 214 Cuts at: 4662 5339 6487 6732 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 918 677 636 526 465 245 214 146 100 51 NaeI GCC'GGC Cuts at: 4442 4442 Size: 7246 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5755 Fragments arranged by size: 5755 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7246 NgoMIV G'CCGG_C Cuts at: 4440 4440 Size: 7246 NheI G'CTAG_C Cuts at: 1052 2375 1052 Size: 1323 5923 NotI GC'GGCC_GC Cuts at: 4693 4693 Size: 7246 NspI r_CATG'y Cuts at: 1132 1229 3265 4604 6149 1132 Size: 97 2036 1339 1545 2229 Fragments arranged by size: 2229 2036 1545 1339 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7246 PciI A'CATG_T Cuts at: 6145 6145 Size: 7246 PflMI CCAn_nnn'nTGG Cuts at: 1108 3118 3142 3588 1108 Size: 2010 24 446 4766 Fragments arranged by size: 4766 2010 446 24 PfoI T'CCnGG_A Cuts at: 2271 2271 Size: 7246 PmeI GTTT'AAAC Cuts at: 4550 4550 Size: 7246 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5315 5347 6828 6860 5315 Size: 32 1481 32 5701 Fragments arranged by size: 5701 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3970 3970 Size: 7246 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7246 PsiI TTA'TAA Cuts at: 4816 4816 Size: 7246 PspOMI G'GGCC_C Cuts at: 2358 2358 Size: 7246 PstI C_TGCA'G Cuts at: 830 2133 2184 2578 3570 4598 830 Size: 1303 51 394 992 1028 3478 Fragments arranged by size: 3478 1303 1028 992 394 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7246 PvuII CAG'CTG Cuts at: 2524 3050 3661 3712 4348 4448 2524 Size: 526 611 51 636 100 5322 Fragments arranged by size: 5322 636 611 526 100 51 SacI G_AGCT'C Cuts at: 2919 2919 Size: 7246 SalI G'TCGA_C Cuts at: 1936 4588 1936 Size: 2652 4594 SbfI CC_TGCA'GG Cuts at: 4598 4598 Size: 7246 ScaI AGT'ACT Cuts at: 3564 3868 5494 7206 3564 Size: 304 1626 1712 3604 Fragments arranged by size: 3604 1712 1626 304 SexAI A'CCwGG_T Cuts at: 2232 2232 Size: 7246 SfcI C'TryA_G Cuts at: 826 1045 2129 2180 2574 3566 4594 5729 6410 Size: 219 1084 51 394 992 1028 1135 681 Cuts at: 6410 6601 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1084 1028 992 681 394 219 191 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7246 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7246 SmaI CCC'GGG Cuts at: 1872 4573 1872 Size: 2701 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4480 5350 6251 6513 820 Size: 197 926 2537 870 901 262 1553 Fragments arranged by size: 2537 1553 926 901 870 262 197 SnaBI TAC'GTA Cuts at: 493 5037 493 Size: 4544 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7246 SphI G_CATG'C Cuts at: 1132 4604 1132 Size: 3472 3774 SspI AAT'ATT Cuts at: 5 52 5170 5 Size: 47 5118 2081 Fragments arranged by size: 5118 2081 47 StuI AGG'CCT Cuts at: 1517 4005 1517 Size: 2488 4758 StyI C'CwwG_G Cuts at: 513 1065 2004 2596 2808 3117 3356 4685 513 Size: 552 939 592 212 309 239 1329 3074 Fragments arranged by size: 3074 1329 939 592 552 309 239 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5273 5290 5443 5628 6073 325 Size: 1255 3693 17 153 185 445 1498 Fragments arranged by size: 3693 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3562 3866 5492 Size: 274 80 33 51 1443 1585 304 1626 Cuts at: 5492 7204 96 Size: 1712 138 Fragments arranged by size: 1712 1626 1585 1443 304 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7206 7238 7206 Size: 32 7214 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7246 XbaI T'CTAG_A Cuts at: 4582 4582 Size: 7246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2218 2821 4225 4420 2218 Size: 603 1404 195 5044 Fragments arranged by size: 5044 1404 603 195 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7246 XmaI C'CCGG_G Cuts at: 1870 4571 1870 Size: 2701 4545 XmnI GAAnn'nnTTC Cuts at: 5375 5375 Size: 7246 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5054 276 Size: 53 83 186 936 3520 2468 Fragments arranged by size: 3520 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI