(Linear) (Six-base) MAPSORT of: KIEE1550.seq Check: 6705 from: 1 to: 2541 KIEE1550.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. Acc65I G'GTAC_C Cuts at: 0 1536 2541 Size: 1536 1005 AclI AA'CG_TT Cuts at: 0 288 362 2541 Size: 288 74 2179 Fragments arranged by size: 2179 288 74 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 734 1228 1508 2281 2541 Size: 337 397 494 280 773 260 Fragments arranged by size: 773 494 397 337 280 260 AgeI A'CCGG_T Cuts at: 0 2069 2541 Size: 2069 472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 200 2541 Size: 200 2341 AlwNI CAG_nnn'CTG Cuts at: 0 123 303 498 519 763 1707 1807 2541 Size: 123 180 195 21 244 944 100 734 Fragments arranged by size: 944 734 244 195 180 123 100 21 ApaI G_GGCC'C Cuts at: 0 357 2541 Size: 357 2184 ApaLI G'TGCA_C Cuts at: 0 1269 2541 Size: 1269 1272 ApoI r'AATT_y Cuts at: 0 625 640 2541 Size: 625 15 1901 Fragments arranged by size: 1901 625 15 AvaI C'yCGr_G Cuts at: 0 134 2541 Size: 134 2407 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2131 2164 2541 Size: 2131 33 377 Fragments arranged by size: 2131 377 33 BamHI G'GATC_C Cuts at: 0 1419 2541 Size: 1419 1122 BanI G'GyrC_C Cuts at: 0 1536 2541 Size: 1536 1005 BanII G_rGCy'C Cuts at: 0 357 914 2541 Size: 357 557 1627 Fragments arranged by size: 1627 557 357 BbsI GAAGACnn'nnnn_ Cuts at: 0 325 1741 2541 Size: 325 1416 800 Fragments arranged by size: 1416 800 325 BbvCI CC'TCA_GC Cuts at: 0 725 1000 2228 2541 Size: 725 275 1228 313 Fragments arranged by size: 1228 725 313 275 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1603 1637 1984 2018 2541 Size: 1603 34 347 34 523 Fragments arranged by size: 1603 523 347 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 213 1976 2132 2541 Size: 213 1763 156 409 Fragments arranged by size: 1763 409 213 156 BglI GCCn_nnn'nGGC Cuts at: 0 1630 2541 Size: 1630 911 BglII A'GATC_T Cuts at: 0 575 2541 Size: 575 1966 Bme1580I G_kGCm'C Cuts at: 0 357 1273 2541 Size: 357 916 1268 Fragments arranged by size: 1268 916 357 BmgBI CAC'GTC Cuts at: 0 2209 2541 Size: 2209 332 BmtI G_CTAG'C Cuts at: 0 374 2541 Size: 374 2167 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1053 2541 Size: 1053 1488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 951 1028 1564 1657 2290 2465 2541 Size: 250 701 77 536 93 633 175 76 Fragments arranged by size: 701 633 536 250 175 93 77 76 Bpu10I CC'TnA_GC Cuts at: 0 725 913 1000 2228 2234 2541 Size: 725 188 87 1228 6 307 Fragments arranged by size: 1228 725 307 188 87 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2460 2541 Size: 2460 81 BsaI GGTCTCn'nnnn_ Cuts at: 0 2039 2541 Size: 2039 502 BsaWI w'CCGG_w Cuts at: 0 2069 2103 2135 2180 2541 Size: 2069 34 32 45 361 Fragments arranged by size: 2069 361 45 34 32 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 784 814 901 931 2541 Size: 784 30 87 30 1610 Fragments arranged by size: 1610 784 87 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1045 1087 1090 1099 1102 1105 1441 1522 Size: 1045 42 3 9 3 3 336 81 Cuts at: 1522 1525 1528 1531 1534 2541 Size: 3 3 3 3 1007 Fragments arranged by size: 1045 1007 336 81 42 9 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 374 1606 1652 1886 1968 2365 2541 Size: 374 1232 46 234 82 397 176 Fragments arranged by size: 1232 397 374 234 176 82 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1343 2541 Size: 1343 1198 BsiHKAI G_wGCw'C Cuts at: 0 344 914 1273 2502 2541 Size: 344 570 359 1229 39 Fragments arranged by size: 1229 570 359 344 39 BsmBI CGTCTCn'nnnn_ Cuts at: 0 55 2541 Size: 55 2486 Bsp1286I G_dGCh'C Cuts at: 0 344 357 914 1273 2502 2541 Size: 344 13 557 359 1229 39 Fragments arranged by size: 1229 557 359 344 39 13 BspEI T'CCGG_A Cuts at: 0 2103 2541 Size: 2103 438 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 168 2541 Size: 168 2373 BsrDI GCAATG_nn' Cuts at: 0 1232 2541 Size: 1232 1309 BsrFI r'CCGG_y Cuts at: 0 1670 2069 2435 2541 Size: 1670 399 366 106 Fragments arranged by size: 1670 399 366 106 BssSI C'ACGA_G Cuts at: 0 482 2541 Size: 482 2059 BstAPI GCAn_nnn'nTGC Cuts at: 0 1707 2541 Size: 1707 834 BstEII G'GTnAC_C Cuts at: 0 67 730 1282 2541 Size: 67 663 552 1259 Fragments arranged by size: 1259 663 552 67 BstXI CCAn_nnnn'nTGG Cuts at: 0 170 2541 Size: 170 2371 BstYI r'GATC_y Cuts at: 0 506 575 1215 1419 2541 Size: 506 69 640 204 1122 Fragments arranged by size: 1122 640 506 204 69 Bsu36I CC'TnA_GG Cuts at: 0 304 1775 2541 Size: 304 1471 766 Fragments arranged by size: 1471 766 304 BtgI C'CryG_G Cuts at: 0 447 1899 1917 2297 2541 Size: 447 1452 18 380 244 Fragments arranged by size: 1452 447 380 244 18 BtsI GCAGTG_nn' Cuts at: 0 1528 1875 2541 Size: 1528 347 666 Fragments arranged by size: 1528 666 347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1800 1835 2541 Size: 1800 35 706 Fragments arranged by size: 1800 706 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1454 2541 Size: 1454 1087 EaeI y'GGCC_r Cuts at: 0 956 2437 2541 Size: 956 1481 104 Fragments arranged by size: 1481 956 104 EarI CTCTTCn'nnn_ Cuts at: 0 827 1076 1144 1874 2031 2257 2541 Size: 827 249 68 730 157 226 284 Fragments arranged by size: 827 730 284 249 226 157 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 514 1156 1689 2541 Size: 514 642 533 852 Fragments arranged by size: 852 642 533 514 EcoICRI GAG'CTC Cuts at: 0 912 2541 Size: 912 1629 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 337 734 951 1028 1228 1508 1564 Size: 250 87 397 217 77 200 280 56 Cuts at: 1564 1657 2281 2290 2465 2541 Size: 93 624 9 175 76 Fragments arranged by size: 624 397 280 250 217 200 175 93 87 77 76 56 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 307 802 2541 Size: 307 495 1739 Fragments arranged by size: 1739 495 307 EcoO109I rG'GnC_Cy Cuts at: 0 300 353 354 1632 1965 2541 Size: 300 53 1 1278 333 576 Fragments arranged by size: 1278 576 333 300 53 1 EcoRV GAT'ATC Cuts at: 0 1362 2541 Size: 1362 1179 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1617 2541 Size: 1617 924 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 376 408 784 816 901 933 1384 1416 Size: 376 32 376 32 85 32 451 32 Cuts at: 1416 1732 1734 1764 1766 1828 1860 2541 Size: 316 2 30 2 62 32 681 Fragments arranged by size: 681 451 376 376 316 85 62 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 0 618 792 2541 Size: 618 174 1749 Fragments arranged by size: 1749 618 174 KpnI G_GTAC'C Cuts at: 0 1540 2541 Size: 1540 1001 MscI TGG'CCA Cuts at: 0 958 2541 Size: 958 1583 MslI CAynn'nnrTG Cuts at: 0 871 892 2541 Size: 871 21 1649 Fragments arranged by size: 1649 871 21 MspA1I CmG'CkG Cuts at: 0 519 1045 1191 1656 1707 2343 2443 2541 Size: 519 526 146 465 51 636 100 98 Fragments arranged by size: 636 526 519 465 146 100 98 51 NaeI GCC'GGC Cuts at: 0 2437 2541 Size: 2437 104 NgoMIV G'CCGG_C Cuts at: 0 2435 2541 Size: 2435 106 NheI G'CTAG_C Cuts at: 0 370 2541 Size: 370 2171 NspI r_CATG'y Cuts at: 0 1260 2541 Size: 1260 1281 PflMI CCAn_nnn'nTGG Cuts at: 0 1113 1137 1583 2541 Size: 1113 24 446 958 Fragments arranged by size: 1113 958 446 24 PfoI T'CCnGG_A Cuts at: 0 266 2541 Size: 266 2275 PpuMI rG'GwC_Cy Cuts at: 0 1965 2541 Size: 1965 576 PspOMI G'GGCC_C Cuts at: 0 353 2541 Size: 353 2188 PstI C_TGCA'G Cuts at: 0 128 179 573 1565 2541 Size: 128 51 394 992 976 Fragments arranged by size: 992 976 394 128 51 PvuII CAG'CTG Cuts at: 0 519 1045 1656 1707 2343 2443 2541 Size: 519 526 611 51 636 100 98 Fragments arranged by size: 636 611 526 519 100 98 51 SacI G_AGCT'C Cuts at: 0 914 2541 Size: 914 1627 ScaI AGT'ACT Cuts at: 0 1559 1863 2541 Size: 1559 304 678 Fragments arranged by size: 1559 678 304 SexAI A'CCwGG_T Cuts at: 0 227 2541 Size: 227 2314 SfcI C'TryA_G Cuts at: 0 124 175 569 1561 2541 Size: 124 51 394 992 980 Fragments arranged by size: 992 980 394 124 51 SmlI C'TyrA_G Cuts at: 0 2475 2541 Size: 2475 66 StuI AGG'CCT Cuts at: 0 2000 2541 Size: 2000 541 StyI C'CwwG_G Cuts at: 0 591 803 1112 1351 2541 Size: 591 212 309 239 1190 Fragments arranged by size: 1190 591 309 239 212 TatI w'GTAC_w Cuts at: 0 1557 1861 2541 Size: 1557 304 680 Fragments arranged by size: 1557 680 304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 213 816 2220 2415 2541 Size: 213 603 1404 195 126 Fragments arranged by size: 1404 603 213 195 126 Enzymes that do cut: Acc65I AclI AcuI AgeI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BglI BglII Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII KpnI MscI MslI MspA1I NaeI NgoMIV NheI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI ScaI SexAI SfcI SmlI StuI StyI TatI XcmI Enzymes that do not cut: AarI AatII AccI AfeI AflII AflIII AhdI AleI AloI AscI AseI AsiSI AvrII BbeI BclI BfrBI BlpI BmrI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrGI BssHII BstBI BstZ17I BtgZI ClaI DraI DraIII EagI EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI MmeI NarI NcoI NdeI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI