################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MflI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 295 GTCTAC GT^[AC][GT]AC AcsI 3 GAATTC [GA]^AATT[CT] AflIII 587 ACGTGT A^C[GA][CT]GT AlwNI 70 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 3 GAATTC [GA]^AATT[CT] AseI 303 ATTAAT AT^TAAT AsnI 303 ATTAAT AT^TAAT BalI 110 TGGCCA TGG^CCA BsaAI 586 TACGTG [CT]AC^GT[GA] BsaWI 1019 TCCGGT [AT]^CCGG[AT] BseRI 640 CTCCTC GAGGAG(10/8) BsmI 63 GAATGC GAATGC(1/-1) BspMI 883 ACCTGC ACCTGC(4/8) DraI 1114 TTTAAA TTT^AAA DraII 488 GGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 310 GACCACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 110 TGGCCA [CT]^GGCC[GA] EcoO109I 488 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 3 GAATTC G^AATTC EcoRV 930 GATATC GAT^ATC EcoT14I 107 CCTTGG C^C[AT][AT]GG HapII 1020 CCGG C^CGG HincII 943 GTCAAC GT[CT]^[GA]AC HindII 943 GTCAAC GT[CT]^[GA]AC HpaII 1020 CCGG C^CGG HphI 325 GGTGA GGTGA(8/7) MaeII 587 ACGT A^CGT MluNI 110 TGGCCA TGG^CCA MscI 110 TGGCCA TGG^CCA MspA1I 418 CAGCTG C[AC]G^C[GT]G MspI 1020 CCGG C^CGG MwoI 136 GCTTATGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 459 ACATGC [GA]CATG^[CT] PaeR7I 434 CTCGAG C^TCGAG PflMI 69 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 613 GACTC GAGTC(4/5) PpuMI 488 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 303 ATTAAT AT^TAAT PstI 652 CTGCAG CTGCA^G PvuII 418 CAGCTG CAG^CTG SfcI 652 CTGCAG C^T[GA][CT]AG SmlI 434 CTCGAG C^T[CT][GA]AG StyI 107 CCTTGG C^C[AT][AT]GG TaqI 435 TCGA T^CGA TthHB8I 435 TCGA T^CGA Van91I 69 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 434 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 573 GTAC GT^AC 756 GTAC AvaI 434 CTCGAG C^[CT]CG[GA]G 1025 CTCGGG BbvI 651 GCTGC GCAGC(8/12) 792 GCTGC BsaI 444 GAGACC GGTCTC(1/5) 1022 GGTCTC BsaJI 20 CCCAGG C^C[ACGT][ACGT]GG 107 CCTTGG BsoBI 434 CTCGAG C^[CT]CG[GA]G 1025 CTCGGG BssKI 21 CCAGG ^CC[ACGT]GG 174 CCTGG BstF5I 228 CATCC GGATG(2/0) 1029 GGATG BstNI 21 CCAGG CC^[AT]GG 174 CCTGG EcoRII 21 CCAGG ^CC[AT]GG 174 CCTGG Fnu4HI 651 GCTGC GC^[ACGT]GC 792 GCTGC FokI 228 CATCC GGATG(9/13) 1029 GGATG HaeIII 111 GGCC GG^CC 177 GGCC ItaI 651 GCTGC GC^[ACGT]GC 792 GCTGC MaeIII 220 GTTAC ^GT[ACGT]AC 559 GTAAC MvaI 21 CCAGG CC^[AT]GG 174 CCTGG RsaI 573 GTAC GT^AC 756 GTAC ScrFI 21 CCAGG CC^[ACGT]GG 174 CCTGG SfaNI 568 GATGC GCATC(5/9) 1062 GATGC TseI 651 GCTGC G^C[AT]GC 792 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI AvaII BclI BsaBI BsiYI BslI BsmAI BsrDI BsrI Cac8I Cfr13I DdeI DpnII FbaI HinfI MamI MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV Sau3AI Sau96I TfiI Tru9I Tsp509I TspRI XcmI