(Circular) (Six-base) MAPSORT of: pFN21AE9528.seq Check: 7485 from: 1 to: 5857 pFN21AE9528 5857 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2301 3200 1937 Size: 364 899 4594 Fragments arranged by size: 4594 899 364 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 5304 5572 1632 Size: 2288 1384 268 1917 Fragments arranged by size: 2288 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 2592 4756 5733 1203 Size: 1389 2164 977 1327 Fragments arranged by size: 2164 1389 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 2080 3600 5172 2080 Size: 1520 1572 2765 Fragments arranged by size: 2765 1572 1520 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 2008 3166 3392 3544 5653 1493 Size: 515 1158 226 152 2109 1697 Fragments arranged by size: 2109 1697 1158 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 2309 4411 160 Size: 872 1277 2102 1606 Fragments arranged by size: 2102 1606 1277 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2439 3030 3182 1870 Size: 73 496 591 152 4545 Fragments arranged by size: 4545 591 496 152 73 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3178 4633 618 Size: 325 217 627 1391 1455 1842 Fragments arranged by size: 1842 1455 1391 627 325 217 BanII G_rGCy'C Cuts at: 1391 3176 1391 Size: 1785 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 2154 2187 2602 1548 Size: 606 33 415 4803 Fragments arranged by size: 4803 606 415 33 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4468 136 Size: 107 122 71 631 605 2796 1525 Fragments arranged by size: 2796 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 3918 5074 1790 Size: 2128 1156 2573 Fragments arranged by size: 2573 2128 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2329 3104 3588 Size: 416 53 83 399 24 912 775 484 Cuts at: 3588 4546 442 Size: 958 1753 Fragments arranged by size: 1753 958 912 775 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4517 1239 Size: 3278 2579 Bpu10I CC'TnA_GC Cuts at: 2131 2131 Size: 5857 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2443 3033 4520 882 Size: 617 944 590 1487 2219 Fragments arranged by size: 2219 1487 944 617 590 BsaAI yAC'GTr Cuts at: 493 1204 2593 3648 493 Size: 711 1389 1055 2702 Fragments arranged by size: 2702 1389 1055 711 BsaBI GATnn'nnATC Cuts at: 2103 2192 2868 5851 2103 Size: 89 676 2983 2109 Fragments arranged by size: 2983 2109 676 89 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3664 4046 4681 275 Size: 53 83 186 936 2131 382 635 1451 Fragments arranged by size: 2131 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3024 4290 4688 4962 5109 5520 3024 Size: 1266 398 274 147 411 3361 Fragments arranged by size: 3361 1266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5632 Fragments arranged by size: 5632 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 1186 Size: 1450 4407 BseYI C'CCAG_C Cuts at: 2073 5060 5556 2073 Size: 2987 496 2374 Fragments arranged by size: 2987 2374 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3918 4003 5074 3918 Size: 85 1071 4701 Fragments arranged by size: 4701 1071 85 BsmI GAATG_Cn' Cuts at: 2074 3368 3461 2074 Size: 1294 93 4470 Fragments arranged by size: 4470 1294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3176 3918 4003 5074 1391 Size: 399 1386 742 85 1071 2174 Fragments arranged by size: 2174 1386 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2897 3212 844 Size: 2053 315 3489 Fragments arranged by size: 3489 2053 315 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2369 2403 2880 2937 4352 4526 65 Size: 1183 1121 34 477 57 1415 174 1396 Fragments arranged by size: 1415 1396 1183 1121 477 174 57 34 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5857 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5857 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstYI r'GATC_y Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852 Size: 1215 367 385 17 1452 86 12 346 Cuts at: 5852 1972 Size: 1977 Fragments arranged by size: 1977 1452 1215 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5305 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2351 487 Size: 683 56 1125 3993 Fragments arranged by size: 3993 1125 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3121 3161 3121 Size: 40 5817 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2321 4864 809 Size: 507 1005 2543 1802 Fragments arranged by size: 2543 1802 1005 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 3304 4193 Size: 54 1425 50 275 18 285 1189 889 Cuts at: 4193 5822 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1189 889 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3787 1353 Size: 2434 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3920 4517 5304 5572 1239 Size: 393 2288 597 787 268 1524 Fragments arranged by size: 2288 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2494 2494 Size: 5857 EcoRI G'AATT_C Cuts at: 2008 3166 3544 2008 Size: 1158 378 4321 Fragments arranged by size: 4321 1158 378 EcoRV GAT'ATC Cuts at: 2937 2937 Size: 5857 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 5004 1678 Size: 3326 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2139 2171 3011 3043 4498 Size: 32 135 32 567 32 840 32 1455 Cuts at: 4498 4530 4572 4604 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1455 840 567 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2950 3201 3447 669 Size: 988 281 1012 251 246 3079 Fragments arranged by size: 3079 1012 988 281 251 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 10 Size: 54 1425 343 285 3750 Fragments arranged by size: 3750 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 2408 2469 2930 3075 3815 4174 Size: 648 1143 99 61 461 145 740 359 Cuts at: 4174 4333 518 Size: 159 2042 Fragments arranged by size: 2042 1143 740 648 461 359 159 145 99 61 MspA1I CmG'CkG Cuts at: 1606 2425 3273 3950 5098 5343 1606 Size: 819 848 677 1148 245 2120 Fragments arranged by size: 2120 1148 848 819 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5305 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 2468 3215 4760 1132 Size: 97 1239 747 1545 2229 Fragments arranged by size: 2229 1545 1239 747 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2080 1108 Size: 972 4885 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2494 2494 Size: 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspXI vC'TCGA_Gb Cuts at: 2439 2439 Size: 5857 PstI C_TGCA'G Cuts at: 830 2661 3209 830 Size: 1831 548 3478 Fragments arranged by size: 3478 1831 548 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2425 2425 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2657 3205 4340 5021 5212 826 Size: 219 1612 548 1135 681 191 1471 Fragments arranged by size: 1612 1471 1135 681 548 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 3184 1872 Size: 1312 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2439 3961 4862 5124 820 Size: 197 926 496 1522 901 262 1553 Fragments arranged by size: 1553 1522 926 901 496 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 2112 3296 513 Size: 552 1047 1184 3074 Fragments arranged by size: 3074 1184 1047 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3884 3901 4054 4239 4684 325 Size: 1255 2304 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2760 4103 5815 Size: 274 80 33 51 1443 783 1343 1712 Cuts at: 5815 96 Size: 138 Fragments arranged by size: 1712 1443 1343 783 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2324 2392 2647 2324 Size: 68 255 5534 Fragments arranged by size: 5534 255 68 XhoI C'TCGA_G Cuts at: 1943 2439 1943 Size: 496 5361 XmaI C'CCGG_G Cuts at: 1870 3182 1870 Size: 1312 4545 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI