################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 80 GTCGAC GT^[AC][GT]AC AlwI 2259 GGATC GGATC(4/5) AocI 652 CCTCAGG CC^T[ACGT]AGG BanII 613 GGGCTC G[GA]GC[CT]^C BbrPI 644 CACGTG CAC^GTG BcnI 1580 CCCGG CC^[GC]GG BsaAI 644 CACGTG [CT]AC^GT[GA] BsgI 200 GTGCAG GTGCAG(16/14) BsmBI 1473 GAGACG CGTCTC(1/5) BspLU11I 1327 ACATGT A^CATGT BsrFI 1899 GCCGGT [GA]^CCGG[CT] BstAPI 600 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1568 CCATTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 652 CCTCAGG CC^T[ACGT]AGG Cfr10I 1899 GCCGGT [GA]^CCGG[CT] DraI 1192 TTTAAA TTT^AAA Eco81I 652 CCTCAGG CC^T[ACGT]AGG EcoNI 1414 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 16 GAATTC G^AATTC EcoRV 1036 GATATC GAT^ATC EcoT14I 751 CCTTGG C^C[AT][AT]GG EcoT22I 1180 ATGCAT ATGCA^T MaeII 645 ACGT A^CGT MspA1I 1562 CAGCTG C[AC]G^C[GT]G NciI 1580 CCCGG CC^[GC]GG NheI 1584 GCTAGC G^CTAGC NsiI 1180 ATGCAT ATGCA^T PmaCI 644 CACGTG CAC^GTG PmlI 644 CACGTG CAC^GTG PpuMI 1287 AGGACCC [GA]G^G[AT]CC[CT] PstI 598 CTGCAG CTGCA^G PvuII 1562 CAGCTG CAG^CTG SalI 80 GTCGAC G^TCGAC StyI 751 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 2043 CCGC CCGC(-3/-1) 2001 GCGG AflIII 643 ACACGT A^C[GA][CT]GT 1327 ACATGT AspHI 848 GTGCTC G[AT]GC[AT]^C 2216 GAGCAC BalI 70 TGGCCA TGG^CCA 1023 TGGCCA BbsI 918 GAAGAC GAAGAC(2/6) 562 GTCTTC BciVI 1105 GGATAC GTATCC(6/5) 2011 GGATAC BpuAI 918 GAAGAC GAAGAC(2/6) 562 GTCTTC BsaJI 751 CCTTGG C^C[ACGT][ACGT]GG 2198 CCTGGG BsiHKAI 848 GTGCTC G[AT]GC[AT]^C 2216 GAGCAC BsmFI 1088 GTCCC GGGAC(10/14) 1355 GTCCC BsmI 1458 GCATTC GAATGC(1/-1) 1922 GCATTC BspHI 525 TCATGA T^CATGA 857 TCATGA BsrDI 1494 CATTGC GCAATG(2/0) 1536 CATTGC DraII 888 GGGGCCT [GA]G^G[ACGT]CC[CT] 1287 AGGACCC EaeI 70 TGGCCA [CT]^GGCC[GA] 1023 TGGCCA EcoO109I 888 GGGGCCT [GA]G^G[ACGT]CC[CT] 1287 AGGACCC HapII 1581 CCGG C^CGG 1900 CCGG HgaI 1475 GACGC GACGC(5/10) 1824 GCGTC HincII 80 GTCGAC GT[CT]^[GA]AC 2164 GTCAAC HindII 80 GTCGAC GT[CT]^[GA]AC 2164 GTCAAC HpaII 1581 CCGG C^CGG 1900 CCGG MluNI 70 TGGCCA TGG^CCA 1023 TGGCCA MscI 70 TGGCCA TGG^CCA 1023 TGGCCA MspI 1581 CCGG C^CGG 1900 CCGG NspI 1178 ACATGC [GA]CATG^[CT] 1327 ACATGT RcaI 525 TCATGA T^CATGA 857 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I AspI AvaII BbvI BfaI BglII BmyI BpmI BsaI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PflFI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmnI