(Circular) (Six-base) MAPSORT of: pFN21AE6722.seq Check: 9266 from: 1 to: 7000 pFN21AE6722 7000 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4810 278 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2086 4343 1937 Size: 149 2257 4594 Fragments arranged by size: 4594 2257 149 Acc65I G'GTAC_C Cuts at: 4321 4321 Size: 7000 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2554 3691 5063 6447 6715 1632 Size: 922 1137 1372 1384 268 1917 Fragments arranged by size: 1917 1384 1372 1137 922 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6803 AflIII A'CryG_T Cuts at: 1203 2648 3332 5899 6876 1203 Size: 1445 684 2567 977 1327 Fragments arranged by size: 2567 1445 1327 977 684 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7000 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7000 AlwNI CAG_nnn'CTG Cuts at: 4743 6315 4743 Size: 1572 5428 ApaLI G'TGCA_C Cuts at: 5057 6213 5057 Size: 1156 5844 ApoI r'AATT_y Cuts at: 1493 2021 4042 4086 4309 4535 4687 6796 1493 Size: 528 2021 44 223 226 152 2109 1697 Fragments arranged by size: 2109 2021 1697 528 226 223 152 44 AseI AT'TA_AT Cuts at: 160 1032 5554 160 Size: 872 4522 1606 Fragments arranged by size: 4522 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 4325 1870 Size: 73 2382 4545 Fragments arranged by size: 4545 2382 73 BamHI G'GATC_C Cuts at: 4330 4697 4330 Size: 367 6633 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4321 5776 618 Size: 325 217 627 2534 1455 1842 Fragments arranged by size: 2534 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2622 4319 1391 Size: 1231 1697 4072 Fragments arranged by size: 4072 1697 1231 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2560 2930 928 Size: 971 661 370 4998 Fragments arranged by size: 4998 971 661 370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5191 5225 1903 Size: 34 3254 34 3678 Fragments arranged by size: 3678 3254 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3104 4010 4892 6102 3104 Size: 906 882 1210 4002 Fragments arranged by size: 4002 1210 906 882 BclI T'GATC_A Cuts at: 1548 1548 Size: 7000 BfrBI ATG'CAT Cuts at: 3187 3187 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5611 136 Size: 107 122 71 631 605 3939 1525 Fragments arranged by size: 3939 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2728 3761 3835 6995 2728 Size: 1033 74 3160 2733 Fragments arranged by size: 3160 2733 1033 74 BlpI GC'TnA_GC Cuts at: 4417 4417 Size: 7000 Bme1580I G_kGCm'C Cuts at: 1790 5061 6217 1790 Size: 3271 1156 2573 Fragments arranged by size: 3271 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7000 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3520 3838 4061 Size: 416 53 83 399 24 2103 318 223 Cuts at: 4061 4731 5689 442 Size: 670 958 1753 Fragments arranged by size: 2103 1753 958 670 416 399 318 223 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3593 1056 Size: 2537 4463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2729 2849 3098 3126 4081 4239 5660 1239 Size: 1490 120 249 28 955 158 1421 2579 Fragments arranged by size: 2579 1490 1421 955 249 158 120 28 Bpu10I CC'TnA_GC Cuts at: 3373 3632 3373 Size: 259 6741 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2625 3220 3405 5125 5990 6288 2625 Size: 595 185 1720 865 298 3337 Fragments arranged by size: 3337 1720 865 595 298 185 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 2745 2773 3077 3983 5663 882 Size: 617 641 605 28 304 906 1680 2219 Fragments arranged by size: 2219 1680 906 641 617 605 304 28 BsaAI yAC'GTr Cuts at: 493 1204 2651 4791 493 Size: 711 1447 2140 2702 Fragments arranged by size: 2702 2140 1447 711 BsaBI GATnn'nnATC Cuts at: 6994 6994 Size: 7000 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4807 5189 5824 275 Size: 53 83 186 936 3274 382 635 1451 Fragments arranged by size: 3274 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5433 5831 6105 6252 6663 5433 Size: 398 274 147 411 5770 Fragments arranged by size: 5770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6775 Fragments arranged by size: 6775 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7000 BseYI C'CCAG_C Cuts at: 2614 6203 6699 2614 Size: 3589 496 2915 Fragments arranged by size: 3589 2915 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2226 1163 Size: 573 490 5937 Fragments arranged by size: 5937 573 490 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4450 5211 6239 1540 Size: 275 186 2449 761 1028 2301 Fragments arranged by size: 2449 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2857 4225 5061 5146 6217 2857 Size: 1368 836 85 1071 3640 Fragments arranged by size: 3640 1368 1071 836 85 BsmI GAATG_Cn' Cuts at: 3463 3927 4511 4604 3463 Size: 464 584 93 5859 Fragments arranged by size: 5859 584 464 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3472 3472 Size: 7000 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2622 2857 4225 4319 5061 5146 6217 Size: 399 832 235 1368 94 742 85 1071 Cuts at: 6217 1391 Size: 2174 Fragments arranged by size: 2174 1368 1071 832 742 399 235 94 85 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 2530 2862 4889 6619 2530 Size: 332 2027 1730 2911 Fragments arranged by size: 2911 2027 1730 332 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4355 844 Size: 3511 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4887 1394 Size: 429 3064 3507 Fragments arranged by size: 3507 3064 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3498 3540 5495 5669 65 Size: 1183 2250 42 1955 174 1396 Fragments arranged by size: 2250 1955 1396 1183 174 42 BsrFI r'CCGG_y Cuts at: 1700 1810 3904 5644 5831 1700 Size: 110 2094 1740 187 2869 Fragments arranged by size: 2869 2094 1740 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7000 BssSI C'ACGA_G Cuts at: 5060 6072 5060 Size: 1012 5988 BstAPI GCAn_nnn'nTGC Cuts at: 794 2611 794 Size: 1817 5183 BstBI TT'CG_AA Cuts at: 5804 5804 Size: 7000 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7000 BstXI CCAn_nnnn'nTGG Cuts at: 3580 3580 Size: 7000 BstYI r'GATC_y Cuts at: 1972 2728 3761 3835 4330 4697 5082 5099 6551 Size: 756 1033 74 495 367 385 17 1452 Cuts at: 6551 6637 6649 6995 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1033 756 495 385 367 346 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2658 2658 Size: 7000 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6448 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6261 Fragments arranged by size: 6261 683 56 BtsI GCAGTG_nn' Cuts at: 4516 5310 5330 4516 Size: 794 20 6186 Fragments arranged by size: 6186 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6965 DraI TTT'AAA Cuts at: 3199 4304 3199 Size: 1105 5895 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6007 809 Size: 507 4691 1802 Fragments arranged by size: 4691 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 3028 4447 Size: 54 1425 50 275 18 245 953 1419 Cuts at: 4447 5336 6965 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1419 953 889 275 245 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4447 1537 Size: 275 2635 4090 Fragments arranged by size: 4090 2635 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4930 1353 Size: 3577 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4021 4037 5587 5961 6107 387 Size: 1064 138 2432 16 1550 374 146 1280 Fragments arranged by size: 2432 1550 1280 1064 374 146 138 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2554 2729 2849 3098 3126 3691 4081 Size: 393 922 175 120 249 28 565 390 Cuts at: 4081 4239 5063 5660 6447 6715 1239 Size: 158 824 597 787 268 1524 Fragments arranged by size: 1524 922 824 787 597 565 393 390 268 249 175 158 120 28 EcoNI CCTnn'n_nnAGG Cuts at: 3423 3423 Size: 7000 EcoO109I rG'GnC_Cy Cuts at: 2894 3293 2894 Size: 399 6601 EcoRI G'AATT_C Cuts at: 2021 4309 4687 2021 Size: 2288 378 4334 Fragments arranged by size: 4334 2288 378 EcoRV GAT'ATC Cuts at: 3043 3043 Size: 7000 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3780 3783 3780 Size: 3 6997 FspI TGC'GCA Cuts at: 5506 5506 Size: 7000 HaeII r_GCGC'y Cuts at: 1678 6147 1678 Size: 4469 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2037 2069 3442 3474 3832 Size: 32 135 32 465 32 1373 32 358 Cuts at: 3832 3864 5641 5673 5715 5747 1373 Size: 32 1777 32 42 32 2626 Fragments arranged by size: 2626 1777 1373 465 358 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2087 4171 4344 4590 669 Size: 988 281 149 2084 173 246 3079 Fragments arranged by size: 3079 2084 988 281 246 173 149 HindIII A'AGCT_T Cuts at: 748 748 Size: 7000 HpaI GTT'AAC Cuts at: 4590 4590 Size: 7000 KpnI G_GTAC'C Cuts at: 4325 4325 Size: 7000 MfeI C'AATT_G Cuts at: 4599 4599 Size: 7000 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2045 2476 2706 2890 6114 6298 1305 Size: 740 431 230 184 3224 184 2007 Fragments arranged by size: 3224 2007 740 431 230 184 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2077 3030 10 Size: 54 1425 343 245 953 3980 Fragments arranged by size: 3980 1425 953 343 245 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 3119 3337 3468 3488 3578 3690 Size: 648 1293 660 218 131 20 90 112 Cuts at: 3690 4958 5317 5476 518 Size: 1268 359 159 2042 Fragments arranged by size: 2042 1293 1268 660 648 359 218 159 131 112 90 20 MspA1I CmG'CkG Cuts at: 1606 3569 4416 5093 6241 6486 1606 Size: 1963 847 677 1148 245 2120 Fragments arranged by size: 2120 1963 1148 847 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6448 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NheI G'CTAG_C Cuts at: 1052 3589 1052 Size: 2537 4463 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NsiI A_TGCA'T Cuts at: 3189 3189 Size: 7000 NspI r_CATG'y Cuts at: 1132 1229 3187 3336 4358 5903 1132 Size: 97 1958 149 1022 1545 2229 Fragments arranged by size: 2229 1958 1545 1022 149 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7000 PciI A'CATG_T Cuts at: 3332 5899 3332 Size: 2567 4433 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7000 PfoI T'CCnGG_A Cuts at: 3075 3075 Size: 7000 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PmlI CAC'GTG Cuts at: 2651 2651 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5069 5101 6582 6614 5069 Size: 32 1481 32 5455 Fragments arranged by size: 5455 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3293 3293 Size: 7000 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 4570 4570 Size: 7000 PstI C_TGCA'G Cuts at: 830 2607 4352 830 Size: 1777 1745 3478 Fragments arranged by size: 3478 1777 1745 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 PvuII CAG'CTG Cuts at: 3569 3569 Size: 7000 SalI G'TCGA_C Cuts at: 1936 2085 4342 1936 Size: 149 2257 4594 Fragments arranged by size: 4594 2257 149 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 5248 6960 5248 Size: 1712 5288 SfcI C'TryA_G Cuts at: 826 1045 2484 2603 2817 4348 5483 6164 6355 Size: 219 1439 119 214 1531 1135 681 191 Cuts at: 6355 826 Size: 1471 Fragments arranged by size: 1531 1471 1439 1135 681 219 214 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 4327 1872 Size: 2455 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2640 3235 3420 5104 6005 6267 Size: 197 926 697 595 185 1684 901 262 Cuts at: 6267 820 Size: 1553 Fragments arranged by size: 1684 1553 926 901 697 595 262 197 185 SnaBI TAC'GTA Cuts at: 493 4791 493 Size: 4298 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 4358 1132 Size: 3226 3774 SspI AAT'ATT Cuts at: 5 52 4924 5 Size: 47 4872 2081 Fragments arranged by size: 4872 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7000 StyI C'CwwG_G Cuts at: 513 1065 2756 4439 513 Size: 552 1691 1683 3074 Fragments arranged by size: 3074 1691 1683 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3113 5027 5044 5197 5382 5827 325 Size: 1255 1533 1914 17 153 185 445 1498 Fragments arranged by size: 1914 1533 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2701 2834 5246 Size: 274 80 33 51 1443 724 133 2412 Cuts at: 5246 6958 96 Size: 1712 138 Fragments arranged by size: 2412 1712 1443 724 274 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3258 3285 3290 3317 3334 3366 6960 6992 3258 Size: 27 5 27 17 32 3594 32 3266 Fragments arranged by size: 3594 3266 32 32 27 27 17 5 Tth111I GACn'n_nGTC Cuts at: 1316 3357 3663 3844 1316 Size: 2041 306 181 4472 Fragments arranged by size: 4472 2041 306 181 XbaI T'CTAG_A Cuts at: 4336 4336 Size: 7000 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7000 XmaI C'CCGG_G Cuts at: 1870 4325 1870 Size: 2455 4545 XmnI GAAnn'nnTTC Cuts at: 2025 2178 3763 4089 5129 2025 Size: 153 1585 326 1040 3896 Fragments arranged by size: 3896 1585 1040 326 153 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4808 276 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI