(Circular) (Six-base) MAPSORT of: pFN21AB5261.seq Check: 627 from: 1 to: 5311 pFN21AB5261 5311 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2516 2516 Size: 5311 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3121 278 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2412 2469 2654 1937 Size: 475 57 185 4594 Fragments arranged by size: 4594 475 185 57 Acc65I G'GTAC_C Cuts at: 2316 2325 2632 2316 Size: 9 307 4995 Fragments arranged by size: 4995 307 9 AclI AA'CG_TT Cuts at: 1563 3438 3811 1563 Size: 1875 373 3063 Fragments arranged by size: 3063 1875 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2355 3374 4758 5026 1632 Size: 723 1019 1384 268 1917 Fragments arranged by size: 1917 1384 1019 723 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5114 AflIII A'CryG_T Cuts at: 1203 4210 5187 1203 Size: 3007 977 1327 Fragments arranged by size: 3007 1327 977 AgeI A'CCGG_T Cuts at: 4142 4142 Size: 5311 AhdI GACnn_n'nnGTC Cuts at: 4040 4040 Size: 5311 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5311 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5311 AlwNI CAG_nnn'CTG Cuts at: 3054 4626 3054 Size: 1572 3739 ApaI G_GGCC'C Cuts at: 2588 2588 Size: 5311 ApaLI G'TGCA_C Cuts at: 3368 4524 3368 Size: 1156 4155 ApoI r'AATT_y Cuts at: 1493 2620 2846 2998 5107 1493 Size: 1127 226 152 2109 1697 Fragments arranged by size: 2109 1697 1127 226 152 AseI AT'TA_AT Cuts at: 160 1032 3865 160 Size: 872 2833 1606 Fragments arranged by size: 2833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 AvaI C'yCGr_G Cuts at: 1870 1943 2059 2516 2636 1870 Size: 73 116 457 120 4545 Fragments arranged by size: 4545 457 120 116 73 BamHI G'GATC_C Cuts at: 2641 3008 2641 Size: 367 4944 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2073 2136 2316 2325 Size: 325 217 627 267 19 63 180 9 Cuts at: 2325 2567 2632 4087 618 Size: 242 65 1455 1842 Fragments arranged by size: 1842 1455 627 325 267 242 217 180 65 63 19 9 BanII G_rGCy'C Cuts at: 1391 2588 2630 1391 Size: 1197 42 4072 Fragments arranged by size: 4072 1197 42 BbeI G_GCGC'C Cuts at: 2058 2077 2140 2571 2058 Size: 19 63 431 4798 Fragments arranged by size: 4798 431 63 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3502 3536 1903 Size: 34 50 34 1481 34 3678 Fragments arranged by size: 3678 1481 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3203 4413 3203 Size: 1210 4101 BclI T'GATC_A Cuts at: 1548 1548 Size: 5311 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 3922 136 Size: 107 122 71 631 605 391 1859 1525 Fragments arranged by size: 1859 1525 631 605 391 122 107 71 BglII A'GATC_T Cuts at: 5306 5306 Size: 5311 BlpI GC'TnA_GC Cuts at: 2728 2728 Size: 5311 Bme1580I G_kGCm'C Cuts at: 1790 2588 3372 4528 1790 Size: 798 784 1156 2573 Fragments arranged by size: 2573 1156 798 784 BmgBI CAC'GTC Cuts at: 1318 2389 1318 Size: 1071 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3042 4000 442 Size: 416 53 83 399 24 1625 958 1753 Fragments arranged by size: 1753 1625 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2533 3971 1239 Size: 902 392 1438 2579 Fragments arranged by size: 2579 1438 902 392 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3436 4301 4599 3436 Size: 865 298 4148 Fragments arranged by size: 4148 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3974 882 Size: 617 2475 2219 Fragments arranged by size: 2475 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3102 493 Size: 711 1898 2702 Fragments arranged by size: 2702 1898 711 BsaBI GATnn'nnATC Cuts at: 5305 5305 Size: 5311 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2074 2137 2568 Size: 53 83 186 936 522 19 63 431 Cuts at: 2568 3118 3500 4135 275 Size: 550 382 635 1451 Fragments arranged by size: 1451 936 635 550 522 431 382 186 83 63 53 19 BsaWI w'CCGG_w Cuts at: 3744 4142 4416 4563 4974 3744 Size: 398 274 147 411 4081 Fragments arranged by size: 4081 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 2282 2312 1178 Size: 30 165 30 711 30 138 30 4177 Fragments arranged by size: 4177 711 165 138 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2396 1186 Size: 940 270 4101 Fragments arranged by size: 4101 940 270 BseYI C'CCAG_C Cuts at: 2043 2182 2581 4514 5010 2043 Size: 139 399 1933 496 2344 Fragments arranged by size: 2344 1933 496 399 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2488 1163 Size: 573 752 3986 Fragments arranged by size: 3986 752 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2087 2108 2148 2242 2255 2461 Size: 275 186 86 21 40 94 13 206 Cuts at: 2461 2761 3522 4550 1540 Size: 300 761 1028 2301 Fragments arranged by size: 2301 1028 761 300 275 206 186 94 86 40 21 13 BsiHKAI G_wGCw'C Cuts at: 2157 3372 3457 4528 2157 Size: 1215 85 1071 2940 Fragments arranged by size: 2940 1215 1071 85 BsmI GAATG_Cn' Cuts at: 2822 2915 2822 Size: 93 5218 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2588 2630 3372 3457 4528 1391 Size: 399 367 431 42 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 431 399 367 85 42 BspEI T'CCGG_A Cuts at: 4974 4974 Size: 5311 BspHI T'CATG_A Cuts at: 3200 4930 3200 Size: 1730 3581 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 2337 2516 2666 844 Size: 1430 63 179 150 3489 Fragments arranged by size: 3489 1430 179 150 63 BsrBI CCG'CTC Cuts at: 1394 1823 3198 1394 Size: 429 1375 3507 Fragments arranged by size: 3507 1375 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3806 3980 65 Size: 1183 2558 174 1396 Fragments arranged by size: 2558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 2103 2147 2453 3955 4142 1700 Size: 110 205 88 44 306 1502 187 2869 Fragments arranged by size: 2869 1502 306 205 187 110 88 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5311 BssHII G'CGCG_C Cuts at: 1925 2201 1925 Size: 276 5035 BssSI C'ACGA_G Cuts at: 3371 4383 3371 Size: 1012 4299 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5311 BstBI TT'CG_AA Cuts at: 4115 4115 Size: 5311 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5311 BstXI CCAn_nnnn'nTGG Cuts at: 2497 2497 Size: 5311 BstYI r'GATC_y Cuts at: 1972 2641 3008 3393 3410 4862 4948 4960 5306 Size: 669 367 385 17 1452 86 12 346 Cuts at: 5306 1972 Size: 1977 Fragments arranged by size: 1977 1452 669 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2049 2087 2496 513 Size: 552 984 38 409 3328 Fragments arranged by size: 3328 984 552 409 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4572 Fragments arranged by size: 4572 683 56 BtsI GCAGTG_nn' Cuts at: 2827 3621 3641 2827 Size: 794 20 4497 Fragments arranged by size: 4497 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5276 DraI TTT'AAA Cuts at: 2615 2615 Size: 5311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4318 809 Size: 507 3002 1802 Fragments arranged by size: 3002 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2084 2105 2145 Size: 54 1425 50 275 18 254 21 40 Cuts at: 2145 2252 2458 2758 3647 5276 8 Size: 107 206 300 889 1629 43 Fragments arranged by size: 1629 1425 889 300 275 254 206 107 54 50 43 40 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2084 2105 2145 2252 2458 2758 1537 Size: 275 272 21 40 107 206 300 4090 Fragments arranged by size: 4090 300 275 272 206 107 40 21 EarI CTCTTCn'nnn_ Cuts at: 1353 3241 1353 Size: 1888 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3898 4272 4418 387 Size: 1064 138 2309 374 146 1280 Fragments arranged by size: 2309 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2355 2533 3374 3971 4758 5026 Size: 393 509 214 178 841 597 787 268 Cuts at: 5026 1239 Size: 1524 Fragments arranged by size: 1524 841 787 597 509 393 268 214 178 EcoO109I rG'GnC_Cy Cuts at: 2585 2585 Size: 5311 EcoRI G'AATT_C Cuts at: 2620 2998 2620 Size: 378 4933 FspI TGC'GCA Cuts at: 2270 3817 2270 Size: 1547 3764 HaeII r_GCGC'y Cuts at: 1678 2033 2058 2077 2140 2406 2571 4458 1678 Size: 355 25 19 63 266 165 1887 2531 Fragments arranged by size: 2531 1887 355 266 165 63 25 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373 Size: 32 135 32 2380 32 42 32 2626 Fragments arranged by size: 2626 2380 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2655 2901 669 Size: 988 281 717 246 3079 Fragments arranged by size: 3079 988 717 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5311 HpaI GTT'AAC Cuts at: 2901 2901 Size: 5311 KasI G'GCGC_C Cuts at: 2054 2073 2136 2567 2054 Size: 19 63 431 4798 Fragments arranged by size: 4798 431 63 19 KpnI G_GTAC'C Cuts at: 2320 2329 2636 2320 Size: 9 307 4995 Fragments arranged by size: 4995 307 9 MfeI C'AATT_G Cuts at: 2910 2910 Size: 5311 MluI A'CGCG_T Cuts at: 5187 5187 Size: 5311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4425 4609 1305 Size: 3120 184 2007 Fragments arranged by size: 3120 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3489 Fragments arranged by size: 3489 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3269 3628 3787 518 Size: 648 1329 774 359 159 2042 Fragments arranged by size: 2042 1329 774 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2089 2128 2182 2603 2727 3404 4552 Size: 445 38 39 54 421 124 677 1148 Cuts at: 4552 4797 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 445 421 245 124 54 39 38 NaeI GCC'GGC Cuts at: 2017 2149 2455 2017 Size: 132 306 4873 Fragments arranged by size: 4873 306 132 NarI GG'CG_CC Cuts at: 2055 2074 2137 2568 2055 Size: 19 63 431 4798 Fragments arranged by size: 4798 431 63 19 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4759 NdeI CA'TA_TG Cuts at: 387 387 Size: 5311 NgoMIV G'CCGG_C Cuts at: 2015 2147 2453 2015 Size: 132 306 4873 Fragments arranged by size: 4873 306 132 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5311 NotI GC'GGCC_GC Cuts at: 2084 2758 2084 Size: 674 4637 NspI r_CATG'y Cuts at: 1132 1229 2419 2669 4214 1132 Size: 97 1190 250 1545 2229 Fragments arranged by size: 2229 1545 1190 250 97 PasI CC'CwG_GG Cuts at: 1348 2283 1348 Size: 935 4376 PciI A'CATG_T Cuts at: 4210 4210 Size: 5311 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5311 PmeI GTTT'AAAC Cuts at: 2615 2615 Size: 5311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3412 4893 4925 3380 Size: 32 1481 32 3766 Fragments arranged by size: 3766 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5311 PsiI TTA'TAA Cuts at: 2881 2881 Size: 5311 PspOMI G'GGCC_C Cuts at: 2584 2584 Size: 5311 PstI C_TGCA'G Cuts at: 830 2181 2663 830 Size: 1351 482 3478 Fragments arranged by size: 3478 1351 482 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5311 PvuII CAG'CTG Cuts at: 2128 2182 2128 Size: 54 5257 RsrII CG'GwC_CG Cuts at: 2101 2101 Size: 5311 SacII CC_GC'GG Cuts at: 2052 2090 2052 Size: 38 5273 SalI G'TCGA_C Cuts at: 1936 2653 1936 Size: 717 4594 SbfI CC_TGCA'GG Cuts at: 2663 2663 Size: 5311 ScaI AGT'ACT Cuts at: 3559 5271 3559 Size: 1712 3599 SfcI C'TryA_G Cuts at: 826 1045 2177 2290 2659 3794 4475 4666 826 Size: 219 1132 113 369 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 681 369 219 191 113 SfoI GGC'GCC Cuts at: 2056 2075 2138 2569 2056 Size: 19 63 431 4798 Fragments arranged by size: 4798 431 63 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 SgrAI Cr'CCGG_yG Cuts at: 1700 2453 1700 Size: 753 4558 SmaI CCC'GGG Cuts at: 1872 2638 1872 Size: 766 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3415 4316 4578 820 Size: 197 926 1472 901 262 1553 Fragments arranged by size: 1553 1472 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3102 493 Size: 2609 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5311 SphI G_CATG'C Cuts at: 1132 2669 1132 Size: 1537 3774 SspI AAT'ATT Cuts at: 5 52 3235 5 Size: 47 3183 2081 Fragments arranged by size: 3183 2081 47 StuI AGG'CCT Cuts at: 1517 2480 1517 Size: 963 4348 StyI C'CwwG_G Cuts at: 513 1065 2750 513 Size: 552 1685 3074 Fragments arranged by size: 3074 1685 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3338 3355 3508 3693 4138 325 Size: 1255 1758 17 153 185 445 1498 Fragments arranged by size: 1758 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3557 5269 96 Size: 274 80 33 51 1443 1580 1712 138 Fragments arranged by size: 1712 1580 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5271 5303 5271 Size: 32 5279 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5311 XbaI T'CTAG_A Cuts at: 2647 2647 Size: 5311 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2578 2578 Size: 5311 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5311 XmaI C'CCGG_G Cuts at: 1870 2636 1870 Size: 766 4545 XmnI GAAnn'nnTTC Cuts at: 3440 3440 Size: 5311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3119 276 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI SanDI SapI SexAI SfiI SrfI SwaI