################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII BalI BamHI BanI BbeI BbrPI BciVI BglI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MluI MluNI MroI MscI MslI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacI SacII SalI Sau96I SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AclI 1647 AACGTT AA^CGTT AflII 2656 CTTAAG C^TTAAG Asp700I 673 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 2136 CCTAGG C^CTAGG BanII 998 GGGCTC G[GA]GC[CT]^C BcgI 2063 CGACATATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2656 CTTAAG C^TTAAG BlnI 2136 CCTAGG C^CTAGG BmyI 998 GGGCTC G[AGT]GC[ACT]^C BsaAI 334 CACGTA [CT]AC^GT[GA] BsaI 2232 GGTCTC GGTCTC(1/5) BsmBI 546 CGTCTC CGTCTC(1/5) Bsp1286I 998 GGGCTC G[AGT]GC[ACT]^C BsrDI 886 CATTGC GCAATG(2/0) BsrFI 1181 ACCGGC [GA]^CCGG[CT] CfoI 1839 GCGC GCG^C Cfr10I 1181 ACCGGC [GA]^CCGG[CT] DraIII 2185 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1541 CCATGG C^C[GA][CT]GG EaeI 2059 TGGCCG [CT]^GGCC[GA] EcoRV 1591 GATATC GAT^ATC HaeII 1838 AGCGCC [GA]GCGC^[CT] HhaI 1839 GCGC GCG^C HinP1I 1839 GCGC G^CGC NcoI 1541 CCATGG C^CATGG NdeI 2066 CATATG CA^TATG NlaIV 1227 GGTTCC GG[ACGT]^[ACGT]CC NspI 1118 GCATGC [GA]CATG^[CT] Psp1406I 1647 AACGTT AA^CGTT PstI 2344 CTGCAG CTGCA^G SapI 1434 GCTCTTC GCTCTTC(1/4) ScaI 688 AGTACT AGT^ACT SphI 1118 GCATGC GCATG^C SspI 2020 AATATT AAT^ATT StuI 1811 AGGCCT AGG^CCT XbaI 1260 TCTAGA T^CTAGA XcmI 968 CCATTTGGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 673 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AciI 148 CCGC CCGC(-3/-1) 1910 GCGG AlwI 19 GGATC GGATC(4/5) 1481 GGATC AlwNI 89 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 317 CAGTGGCTG BbsI 220 GAAGAC GAAGAC(2/6) 364 GAAGAC BbvCI 86 CCTCAGC CCTCAGC(-5/-2) 403 GCTGAGG BcnI 2014 CCGGG CC^[GC]GG 2685 CCGGG BglII 115 AGATCT A^GATCT 785 AGATCT BpuAI 220 GAAGAC GAAGAC(2/6) 364 GAAGAC BsaBI 900 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2460 GATAAAGATC BsgI 1024 GTGCAG GTGCAG(16/14) 1715 GTGCAG BsiYI 965 CCTCCATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2043 CCTGACAATGG BslI 965 CCTCCATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2043 CCTGACAATGG BsmI 1906 GAATGC GAATGC(1/-1) 1046 GCATTC Bsp1407I 767 TGTACA T^GTACA 2618 TGTACA BspHI 902 TCATGA T^CATGA 1973 TCATGA BsrGI 767 TGTACA T^GTACA 2618 TGTACA BstBI 1103 TTCGAA TT^CGAA 2564 TTCGAA BstYI 115 AGATCT [GA]^GATC[CT] 785 AGATCT EcoRI 951 GAATTC G^AATTC 1406 GAATTC EcoT14I 1541 CCATGG C^C[AT][AT]GG 2136 CCTAGG HpaI 1249 GTTAAC GTT^AAC 2028 GTTAAC MamI 900 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2460 GATAAAGATC MflI 115 AGATCT [GA]^GATC[CT] 785 AGATCT NciI 2014 CCGGG CC^[GC]GG 2685 CCGGG NspV 1103 TTCGAA TT^CGAA 2564 TTCGAA RcaI 902 TCATGA T^CATGA 1973 TCATGA SfuI 1103 TTCGAA TT^CGAA 2564 TTCGAA SmlI 1747 CTTGAG C^T[CT][GA]AG 2656 CTTAAG SspBI 767 TGTACA T^GTACA 2618 TGTACA StyI 1541 CCATGG C^C[AT][AT]GG 2136 CCTAGG Tsp45I 6 GTCAC ^GT[GC]AC 2414 GTGAC XhoII 115 AGATCT [GA]^GATC[CT] 785 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI BbvI BclI BfaI BpmI BsaJI BseRI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII DraI EarI EcoRII FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MnlI MseI MspA1I MspI MunI MvaI MwoI NdeII NlaIII PshBI PvuII RsaI Sau3AI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I