(Linear) (Six-base) MAPSORT of: KIEE4588.seq Check: 9277 from: 1 to: 2739 KIEE4588.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AclI AA'CG_TT Cuts at: 0 1648 2739 Size: 1648 1091 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 931 1217 1354 1422 1624 2095 2146 2739 Size: 931 286 137 68 202 471 51 593 Fragments arranged by size: 931 593 471 286 202 137 68 51 AflII C'TTAA_G Cuts at: 0 2656 2739 Size: 2656 83 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1448 1480 2739 Size: 1448 32 1259 Fragments arranged by size: 1448 1259 32 AlwNI CAG_nnn'CTG Cuts at: 0 94 322 2739 Size: 94 228 2417 Fragments arranged by size: 2417 228 94 ApoI r'AATT_y Cuts at: 0 951 1136 1406 1615 2203 2632 2739 Size: 951 185 270 209 588 429 107 Fragments arranged by size: 951 588 429 270 209 185 107 AseI AT'TA_AT Cuts at: 0 487 1210 1318 1520 2739 Size: 487 723 108 202 1219 Fragments arranged by size: 1219 723 487 202 108 AvrII C'CTAG_G Cuts at: 0 2136 2739 Size: 2136 603 BanII G_rGCy'C Cuts at: 0 1002 2739 Size: 1002 1737 BbsI GAAGACnn'nnnn_ Cuts at: 0 227 371 2739 Size: 227 144 2368 Fragments arranged by size: 2368 227 144 BbvCI CC'TCA_GC Cuts at: 0 87 404 2739 Size: 87 317 2335 Fragments arranged by size: 2335 317 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2052 2086 2739 Size: 2052 34 653 Fragments arranged by size: 2052 653 34 BclI T'GATC_A Cuts at: 0 593 899 905 1014 1772 2739 Size: 593 306 6 109 758 967 Fragments arranged by size: 967 758 593 306 109 6 BglII A'GATC_T Cuts at: 0 115 785 2739 Size: 115 670 1954 Fragments arranged by size: 1954 670 115 BmrI ACTGGGnnnn_n' Cuts at: 0 74 2739 Size: 74 2665 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 429 2739 Size: 429 2310 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 1006 2212 2739 Size: 394 612 1206 527 Fragments arranged by size: 1206 612 527 394 Bpu10I CC'TnA_GC Cuts at: 0 87 104 404 2739 Size: 87 17 300 2335 Fragments arranged by size: 2335 300 87 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1768 2739 Size: 1768 971 BsaI GGTCTCn'nnnn_ Cuts at: 0 2238 2739 Size: 2238 501 BsaAI yAC'GTr Cuts at: 0 336 2739 Size: 336 2403 BsaBI GATnn'nnATC Cuts at: 0 904 2464 2739 Size: 904 1560 275 Fragments arranged by size: 1560 904 275 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1448 1478 2739 Size: 1448 30 1261 Fragments arranged by size: 1448 1261 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 234 245 405 408 411 414 417 2293 Size: 234 11 160 3 3 3 3 1876 Cuts at: 2293 2739 Size: 446 Fragments arranged by size: 1876 446 234 160 11 3 3 3 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1045 1736 2739 Size: 1045 691 1003 Fragments arranged by size: 1045 1003 691 BsmI GAATG_Cn' Cuts at: 0 1046 1912 2739 Size: 1046 866 827 Fragments arranged by size: 1046 866 827 BsmBI CGTCTCn'nnnn_ Cuts at: 0 552 2739 Size: 552 2187 Bsp1286I G_dGCh'C Cuts at: 0 1002 2739 Size: 1002 1737 BspHI T'CATG_A Cuts at: 0 902 1973 2739 Size: 902 1071 766 Fragments arranged by size: 1071 902 766 BsrDI GCAATG_nn' Cuts at: 0 885 2739 Size: 885 1854 BsrFI r'CCGG_y Cuts at: 0 1181 2739 Size: 1181 1558 BsrGI T'GTAC_A Cuts at: 0 767 2618 2739 Size: 767 1851 121 Fragments arranged by size: 1851 767 121 BstBI TT'CG_AA Cuts at: 0 1104 2565 2739 Size: 1104 1461 174 Fragments arranged by size: 1461 1104 174 BstYI r'GATC_y Cuts at: 0 115 785 2739 Size: 115 670 1954 Fragments arranged by size: 1954 670 115 BtgI C'CryG_G Cuts at: 0 1541 2739 Size: 1541 1198 BtsI GCAGTG_nn' Cuts at: 0 674 1483 2341 2739 Size: 674 809 858 398 Fragments arranged by size: 858 809 674 398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2130 2165 2739 Size: 2130 35 574 Fragments arranged by size: 2130 574 35 DraI TTT'AAA Cuts at: 0 606 857 1382 1566 1947 2208 2739 Size: 606 251 525 184 381 261 531 Fragments arranged by size: 606 531 525 381 261 251 184 DraIII CAC_nnn'GTG Cuts at: 0 2190 2739 Size: 2190 549 EaeI y'GGCC_r Cuts at: 0 2059 2739 Size: 2059 680 EarI CTCTTCn'nnn_ Cuts at: 0 160 243 252 498 1427 1441 2288 2656 Size: 160 83 9 246 929 14 847 368 Cuts at: 2656 2739 Size: 83 Fragments arranged by size: 929 847 368 246 160 83 83 14 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 137 2739 Size: 137 2602 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 931 1006 1217 1354 1422 1624 2095 Size: 394 537 75 211 137 68 202 471 Cuts at: 2095 2146 2212 2739 Size: 51 66 527 Fragments arranged by size: 537 527 471 394 211 202 137 75 68 66 51 EcoRI G'AATT_C Cuts at: 0 951 1406 2739 Size: 951 455 1333 Fragments arranged by size: 1333 951 455 EcoRV GAT'ATC Cuts at: 0 1593 2739 Size: 1593 1146 HaeII r_GCGC'y Cuts at: 0 1842 2739 Size: 1842 897 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 100 132 220 252 265 297 884 916 Size: 100 32 88 32 13 32 587 32 Cuts at: 916 2739 Size: 1823 Fragments arranged by size: 1823 587 100 88 32 32 32 32 13 HincII GTy'rAC Cuts at: 0 1251 1578 2030 2174 2739 Size: 1251 327 452 144 565 Fragments arranged by size: 1251 565 452 327 144 HpaI GTT'AAC Cuts at: 0 1251 2030 2739 Size: 1251 779 709 Fragments arranged by size: 1251 779 709 MfeI C'AATT_G Cuts at: 0 1037 1085 2558 2739 Size: 1037 48 1473 181 Fragments arranged by size: 1473 1037 181 48 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1739 1830 2739 Size: 1739 91 909 Fragments arranged by size: 1739 909 91 MspA1I CmG'CkG Cuts at: 0 91 777 1195 1678 2739 Size: 91 686 418 483 1061 Fragments arranged by size: 1061 686 483 418 91 NcoI C'CATG_G Cuts at: 0 1541 2739 Size: 1541 1198 NdeI CA'TA_TG Cuts at: 0 2067 2739 Size: 2067 672 NspI r_CATG'y Cuts at: 0 1122 2739 Size: 1122 1617 PasI CC'CwG_GG Cuts at: 0 269 2739 Size: 269 2470 PfoI T'CCnGG_A Cuts at: 0 2013 2739 Size: 2013 726 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1442 1448 1474 1480 2739 Size: 1442 6 26 6 1259 Fragments arranged by size: 1442 1259 26 6 6 PsiI TTA'TAA Cuts at: 0 1009 2739 Size: 1009 1730 PstI C_TGCA'G Cuts at: 0 2348 2739 Size: 2348 391 PvuII CAG'CTG Cuts at: 0 91 777 1195 1678 2739 Size: 91 686 418 483 1061 Fragments arranged by size: 1061 686 483 418 91 SapI GCTCTTCn'nnn_ Cuts at: 0 1441 2739 Size: 1441 1298 ScaI AGT'ACT Cuts at: 0 690 2739 Size: 690 2049 SfcI C'TryA_G Cuts at: 0 728 1328 1733 1865 2344 2513 2739 Size: 728 600 405 132 479 169 226 Fragments arranged by size: 728 600 479 405 226 169 132 SmlI C'TyrA_G Cuts at: 0 1747 2656 2739 Size: 1747 909 83 Fragments arranged by size: 1747 909 83 SphI G_CATG'C Cuts at: 0 1122 2739 Size: 1122 1617 SspI AAT'ATT Cuts at: 0 2022 2739 Size: 2022 717 StuI AGG'CCT Cuts at: 0 1813 2739 Size: 1813 926 StyI C'CwwG_G Cuts at: 0 1541 2136 2739 Size: 1541 595 603 Fragments arranged by size: 1541 603 595 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1321 2739 Size: 1321 1418 TatI w'GTAC_w Cuts at: 0 688 701 767 2618 2723 2739 Size: 688 13 66 1851 105 16 Fragments arranged by size: 1851 688 105 66 16 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2174 2206 2739 Size: 2174 32 533 Fragments arranged by size: 2174 533 32 XbaI T'CTAG_A Cuts at: 0 1260 2739 Size: 1260 1479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 975 2739 Size: 975 1764 XmnI GAAnn'nnTTC Cuts at: 0 677 2739 Size: 677 2062 Enzymes that do cut: AclI AcuI AflII AloI AlwNI ApoI AseI AvrII BanII BbsI BbvCI BcgI BclI BglII BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsgI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BsrGI BstBI BstYI BtgI BtsI CspCI DraI DraIII EaeI EarI EciI Eco57MI EcoRI EcoRV HaeII Hin4I HincII HpaI MfeI MmeI MspA1I NcoI NdeI NspI PasI PfoI PpiI PsiI PstI PvuII SapI ScaI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvaI BaeI BamHI BanI BbeI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DrdI EagI EcoICRI EcoNI EcoO109I FalI FseI FspI FspAI HindIII KasI KpnI MluI MscI MslI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI ZraI