Restriction map for
pFN21AE5714
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BlnI BsaI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HpaI KspI MluI MroI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SgrAI SpeI SphI Sse8387I
StyI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 235 GACGTC GACGT^C
Acc65I 572 GGTACC G^GTACC
AccI 1609 GTAGAC GT^[AC][GT]AC
AccII 2131 CGCG CG^CG
AflII 552 CTTAAG C^TTAAG
Alw44I 2008 GTGCAC G^TGCAC
AocI 211 CCTCAGG CC^T[ACGT]AGG
ApaLI 2008 GTGCAC G^TGCAC
Asp718I 572 GGTACC G^GTACC
AvaI 106 CCCGGG C^[CT]CG[GA]G
BalI 54 TGGCCA TGG^CCA
BamHI 1506 GGATCC G^GATCC
BbeI 44 GGCGCC GGCGC^C
BbsI 1526 GTCTTC GAAGAC(2/6)
BfrI 552 CTTAAG C^TTAAG
BglI 47 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1526 GTCTTC GAAGAC(2/6)
BsaAI 536 TACGTA [CT]AC^GT[GA]
BsaBI 1166 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 157 TCCGGT [AT]^CCGG[AT]
BsoBI 106 CCCGGG C^[CT]CG[GA]G
BspHI 1461 TCATGA T^CATGA
BspLU11I 810 ACATGT A^CATGT
BspMI 966 ACCTGC ACCTGC(4/8)
BstAPI 454 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 721 TTCGAA TT^CGAA
BstUI 2131 CGCG CG^CG
BstXI 562 CCACCTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 211 CCTCAGG CC^T[ACGT]AGG
EaeI 54 TGGCCA [CT]^GGCC[GA]
Eco81I 211 CCTCAGG CC^T[ACGT]AGG
FauI 40 GCGGG CCCGC(4/6)
HgaI 2043 GACGC GACGC(5/10)
KasI 44 GGCGCC G^GCGCC
KpnI 572 GGTACC GGTAC^C
MamI 1166 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1277 CAATTG C^AATTG
MluNI 54 TGGCCA TGG^CCA
MscI 54 TGGCCA TGG^CCA
MunI 1277 CAATTG C^AATTG
MvnI 2131 CGCG CG^CG
NarI 44 GGCGCC GG^CGCC
NspV 721 TTCGAA TT^CGAA
PflMI 899 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1461 TCATGA T^CATGA
SapI 769 GAAGAGC GCTCTTC(1/4)
SexAI 980 ACCTGGT A^CC[AT]GGT
SfoI 44 GGCGCC GGC^GCC
SfuI 721 TTCGAA TT^CGAA
SmaI 106 CCCGGG CCC^GGG
SnaBI 536 TACGTA TAC^GTA
StuI 1498 AGGCCT AGG^CCT
Van91I 899 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 106 CCCGGG C^CCGGG
XmaI 106 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 44 GGCGCC G[GA]^CG[CT]C
235 GACGTC
AflIII 179 ACACGT A^C[GA][CT]GT
810 ACATGT
AlwNI 319 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
2300 CAGAGTCTG
ApaI 97 GGGCCC GGGCC^C
216 GGGCCC
Asp700I 657 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
943 GAAGAGGTTC
AspHI 920 GAGCAC G[AT]GC[AT]^C
2008 GTGCAC
BlpI 1540 GCTGAGC GC^T[ACGT]AGC
1920 GCTAAGC
Bpu1102I 1540 GCTGAGC GC^T[ACGT]AGC
1920 GCTAAGC
BsaHI 44 GGCGCC G[GA]^CG[CT]C
235 GACGTC
BsiHKAI 920 GAGCAC G[AT]GC[AT]^C
2008 GTGCAC
BsmBI 352 CGTCTC CGTCTC(1/5)
2041 GAGACG
Bsp1407I 820 TGTACA T^GTACA
998 TGTACA
BsrGI 820 TGTACA T^GTACA
998 TGTACA
CelII 1540 GCTGAGC GC^T[ACGT]AGC
1920 GCTAAGC
CfoI 45 GCGC GCG^C
224 GCGC
DraI 1432 TTTAAA TTT^AAA
1660 TTTAAA
DrdI 229 GACGGCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1102 GACTGCATTGTC
HaeII 44 GGCGCC [GA]GCGC^[CT]
223 AGCGCC
HhaI 45 GCGC GCG^C
224 GCGC
Hin1I 44 GGCGCC G[GA]^CG[CT]C
235 GACGTC
HinP1I 45 GCGC G^CGC
224 GCGC
HindIII 365 AAGCTT A^AGCTT
375 AAGCTT
PstI 1393 CTGCAG CTGCA^G
1670 CTGCAG
PvuII 1175 CAGCTG CAG^CTG
1520 CAGCTG
SspBI 820 TGTACA T^GTACA
998 TGTACA
XcmI 895 CCAGCCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1049 CCATTTCTGTGCTGG
XmnI 657 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
943 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII