(Circular) (Six-base) MAPSORT of: pFN21AE5714.seq Check: 1825 from: 1 to: 7063 pFN21AE5714 7063 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2244 4873 278 Size: 53 83 186 936 708 2629 2468 Fragments arranged by size: 2629 2468 936 708 186 83 53 AccI GT'mk_AC Cuts at: 1937 3615 4406 1937 Size: 1678 791 4594 Fragments arranged by size: 4594 1678 791 Acc65I G'GTAC_C Cuts at: 2577 4384 2577 Size: 1807 5256 AclI AA'CG_TT Cuts at: 1563 5190 5563 1563 Size: 3627 373 3063 Fragments arranged by size: 3627 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2967 5126 6510 6778 1632 Size: 1335 2159 1384 268 1917 Fragments arranged by size: 2159 1917 1384 1335 268 AflII C'TTAA_G Cuts at: 820 1017 2557 820 Size: 197 1540 5326 Fragments arranged by size: 5326 1540 197 AflIII A'CryG_T Cuts at: 1203 2184 2815 5962 6939 1203 Size: 981 631 3147 977 1327 Fragments arranged by size: 3147 1327 981 977 631 AgeI A'CCGG_T Cuts at: 5894 5894 Size: 7063 AhdI GACnn_n'nnGTC Cuts at: 5792 5792 Size: 7063 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2092 1184 Size: 908 6155 AlwNI CAG_nnn'CTG Cuts at: 2329 4310 4806 6378 2329 Size: 1981 496 1572 3014 Fragments arranged by size: 3014 1981 1572 496 ApaI G_GGCC'C Cuts at: 2106 2225 2106 Size: 119 6944 ApaLI G'TGCA_C Cuts at: 4013 5120 6276 4013 Size: 1107 1156 4800 Fragments arranged by size: 4800 1156 1107 ApoI r'AATT_y Cuts at: 1493 2300 2319 2880 3162 3492 3668 4063 4372 Size: 807 19 561 282 330 176 395 309 Cuts at: 4372 4598 4750 6859 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 807 561 395 330 309 282 226 176 152 19 AseI AT'TA_AT Cuts at: 160 1032 5617 160 Size: 872 4585 1606 Fragments arranged by size: 4585 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 AvaI C'yCGr_G Cuts at: 1870 1943 2111 4388 1870 Size: 73 168 2277 4545 Fragments arranged by size: 4545 2277 168 73 BamHI G'GATC_C Cuts at: 3511 4393 4760 3511 Size: 882 367 5814 Fragments arranged by size: 5814 882 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 2577 4139 4384 5839 Size: 325 217 627 262 528 1562 245 1455 Cuts at: 5839 618 Size: 1842 Fragments arranged by size: 1842 1562 1455 627 528 325 262 245 217 BanII G_rGCy'C Cuts at: 1391 2035 2106 2225 3810 4382 1391 Size: 644 71 119 1585 572 4072 Fragments arranged by size: 4072 1585 644 572 119 71 BbeI G_GCGC'C Cuts at: 2053 2053 Size: 7063 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3524 928 Size: 971 1625 4467 Fragments arranged by size: 4467 1625 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5254 5288 1903 Size: 34 3317 34 3678 Fragments arranged by size: 3678 3317 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4955 6165 4955 Size: 1210 5853 BclI T'GATC_A Cuts at: 1548 1548 Size: 7063 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 5674 136 Size: 107 122 71 631 605 386 3616 1525 Fragments arranged by size: 3616 1525 631 605 386 122 107 71 BglII A'GATC_T Cuts at: 2721 3472 3973 7058 2721 Size: 751 501 3085 2726 Fragments arranged by size: 3085 2726 751 501 BlpI GC'TnA_GC Cuts at: 3546 3926 4480 3546 Size: 380 554 6129 Fragments arranged by size: 6129 554 380 Bme1580I G_kGCm'C Cuts at: 1790 2023 2106 2202 2225 4017 5124 6280 1790 Size: 233 83 96 23 1792 1107 1156 2573 Fragments arranged by size: 2573 1792 1156 1107 233 96 83 23 BmgBI CAC'GTC Cuts at: 1318 2187 1318 Size: 869 6194 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2339 3336 4794 Size: 416 53 83 399 24 922 997 1458 Cuts at: 4794 5752 442 Size: 958 1753 Fragments arranged by size: 1753 1458 997 958 922 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2703 3962 4221 5723 1239 Size: 1464 1259 259 1502 2579 Fragments arranged by size: 2579 1502 1464 1259 259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2512 3207 3375 5188 6053 6351 2512 Size: 695 168 1813 865 298 3224 Fragments arranged by size: 3224 1813 865 695 298 168 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5726 882 Size: 617 4227 2219 Fragments arranged by size: 4227 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2543 4854 493 Size: 711 1339 2311 2702 Fragments arranged by size: 2702 2311 1339 711 BsaBI GATnn'nnATC Cuts at: 3175 7057 3175 Size: 3882 3181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2050 2241 4870 5252 Size: 53 83 186 936 517 191 2629 382 Cuts at: 5252 5887 275 Size: 635 1451 Fragments arranged by size: 2629 1451 936 635 517 382 191 186 83 53 BsaWI w'CCGG_w Cuts at: 2162 5496 5894 6168 6315 6726 2162 Size: 3334 398 274 147 411 2499 Fragments arranged by size: 3334 2499 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2987 3017 1178 Size: 30 165 30 1584 30 5224 Fragments arranged by size: 5224 1584 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2140 2143 4220 1186 Size: 954 3 2077 4029 Fragments arranged by size: 4029 2077 954 3 BseYI C'CCAG_C Cuts at: 2225 4175 6266 6762 2225 Size: 1950 2091 496 2526 Fragments arranged by size: 2526 2091 1950 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2478 2860 3712 4238 1163 Size: 573 742 382 852 526 3988 Fragments arranged by size: 3988 852 742 573 526 382 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4513 5274 6302 1540 Size: 275 186 2512 761 1028 2301 Fragments arranged by size: 2512 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2929 4017 5124 5209 6280 2929 Size: 1088 1107 85 1071 3712 Fragments arranged by size: 3712 1107 1088 1071 85 BsmI GAATG_Cn' Cuts at: 4574 4667 4574 Size: 93 6970 BsmBI CGTCTCn'nnnn_ Cuts at: 2363 4040 2363 Size: 1677 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2035 2106 2202 2225 2929 3810 Size: 399 233 12 71 96 23 704 881 Cuts at: 3810 4017 4382 5124 5209 6280 1391 Size: 207 365 742 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 704 399 365 233 207 96 85 71 23 12 BspEI T'CCGG_A Cuts at: 6726 6726 Size: 7063 BspHI T'CATG_A Cuts at: 3466 4952 6682 3466 Size: 1486 1730 3847 Fragments arranged by size: 3847 1730 1486 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2980 4418 844 Size: 2136 1438 3489 Fragments arranged by size: 3489 2136 1438 BsrBI CCG'CTC Cuts at: 1394 1823 4950 1394 Size: 429 3127 3507 Fragments arranged by size: 3507 3127 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5558 5732 65 Size: 1183 4310 174 1396 Fragments arranged by size: 4310 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5707 5894 1700 Size: 110 3897 187 2869 Fragments arranged by size: 3897 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2825 3003 96 Size: 2729 178 4156 Fragments arranged by size: 4156 2729 178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7063 BssSI C'ACGA_G Cuts at: 5123 6135 5123 Size: 1012 6051 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 794 Size: 1671 5392 BstBI TT'CG_AA Cuts at: 2727 5867 2727 Size: 3140 3923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7063 BstXI CCAn_nnnn'nTGG Cuts at: 2574 2574 Size: 7063 BstYI r'GATC_y Cuts at: 1972 2721 2896 3472 3511 3883 3910 3973 4393 Size: 749 175 576 39 372 27 63 420 Cuts at: 4393 4760 5145 5162 6614 6700 6712 7058 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 749 576 420 385 372 367 346 175 86 63 39 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2217 2217 Size: 7063 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6511 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6324 Fragments arranged by size: 6324 683 56 BtsI GCAGTG_nn' Cuts at: 2848 4579 5373 5393 2848 Size: 1731 794 20 4518 Fragments arranged by size: 4518 1731 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7063 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7028 DraI TTT'AAA Cuts at: 3439 3667 4367 3439 Size: 228 700 6135 Fragments arranged by size: 6135 700 228 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2240 3113 6070 809 Size: 507 924 873 2957 1802 Fragments arranged by size: 2957 1802 924 873 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2059 4510 5399 Size: 54 1425 50 275 18 229 2451 889 Cuts at: 5399 7028 8 Size: 1629 43 Fragments arranged by size: 2451 1629 1425 889 275 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4510 1537 Size: 275 2698 4090 Fragments arranged by size: 4090 2698 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2366 2769 2943 3494 4993 1353 Size: 1013 403 174 551 1499 3423 Fragments arranged by size: 3423 1499 1013 551 403 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5650 6024 6170 387 Size: 1064 138 4061 374 146 1280 Fragments arranged by size: 4061 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2703 2967 3962 4221 5126 5723 6510 Size: 393 1071 264 995 259 905 597 787 Cuts at: 6510 6778 1239 Size: 268 1524 Fragments arranged by size: 1524 1071 995 905 787 597 393 268 264 259 EcoO109I rG'GnC_Cy Cuts at: 2103 2221 2222 2624 2103 Size: 118 1 402 6542 Fragments arranged by size: 6542 402 118 1 EcoRI G'AATT_C Cuts at: 4372 4750 4372 Size: 378 6685 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2308 2308 Size: 7063 FspI TGC'GCA Cuts at: 5569 5569 Size: 7063 HaeII r_GCGC'y Cuts at: 1678 2053 2232 6210 1678 Size: 375 179 3978 2531 Fragments arranged by size: 3978 2531 375 179 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2881 2913 5704 5736 5778 Size: 32 135 32 1309 32 2791 32 42 Cuts at: 5778 5810 1373 Size: 32 2626 Fragments arranged by size: 2791 2626 1309 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4407 4653 669 Size: 988 281 2469 246 3079 Fragments arranged by size: 3079 2469 988 281 246 HindIII A'AGCT_T Cuts at: 748 2370 2380 748 Size: 1622 10 5431 Fragments arranged by size: 5431 1622 10 HpaI GTT'AAC Cuts at: 4653 4653 Size: 7063 KasI G'GCGC_C Cuts at: 2049 2049 Size: 7063 KpnI G_GTAC'C Cuts at: 2581 4388 2581 Size: 1807 5256 MfeI C'AATT_G Cuts at: 3282 4662 3282 Size: 1380 5683 MluI A'CGCG_T Cuts at: 6939 6939 Size: 7063 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2502 3563 6177 6361 1305 Size: 1197 1061 2614 184 2007 Fragments arranged by size: 2614 2007 1197 1061 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2061 10 Size: 54 1425 343 229 5012 Fragments arranged by size: 5012 1425 343 229 54 MslI CAynn'nnrTG Cuts at: 518 1166 2469 2961 3059 5021 5380 5539 518 Size: 648 1303 492 98 1962 359 159 2042 Fragments arranged by size: 2042 1962 1303 648 492 359 159 98 MspA1I CmG'CkG Cuts at: 1606 2602 3182 3527 4479 5156 6304 6549 1606 Size: 996 580 345 952 677 1148 245 2120 Fragments arranged by size: 2120 1148 996 952 677 580 345 245 NarI GG'CG_CC Cuts at: 2050 2050 Size: 7063 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6511 NdeI CA'TA_TG Cuts at: 387 387 Size: 7063 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7063 NotI GC'GGCC_GC Cuts at: 4510 4510 Size: 7063 NspI r_CATG'y Cuts at: 1132 1229 2501 2819 4246 4421 5966 1132 Size: 97 1272 318 1427 175 1545 2229 Fragments arranged by size: 2229 1545 1427 1272 318 175 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7063 PciI A'CATG_T Cuts at: 2815 5962 2815 Size: 3147 3916 PflMI CCAn_nnn'nTGG Cuts at: 1108 2910 1108 Size: 1802 5261 PmeI GTTT'AAAC Cuts at: 4367 4367 Size: 7063 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5132 5164 6645 6677 5132 Size: 32 1481 32 5518 Fragments arranged by size: 5518 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7063 PsiI TTA'TAA Cuts at: 3335 4633 3335 Size: 1298 5765 PspOMI G'GGCC_C Cuts at: 2102 2221 2102 Size: 119 6944 PstI C_TGCA'G Cuts at: 830 3402 3679 4415 830 Size: 2572 277 736 3478 Fragments arranged by size: 3478 2572 736 277 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7063 PvuII CAG'CTG Cuts at: 3182 3527 3182 Size: 345 6718 SalI G'TCGA_C Cuts at: 1936 4405 1936 Size: 2469 4594 SapI GCTCTTCn'nnn_ Cuts at: 2769 2769 Size: 7063 SbfI CC_TGCA'GG Cuts at: 4415 4415 Size: 7063 ScaI AGT'ACT Cuts at: 5311 7023 5311 Size: 1712 5351 SexAI A'CCwGG_T Cuts at: 2985 2985 Size: 7063 SfcI C'TryA_G Cuts at: 826 1045 3273 3398 3675 4411 5546 6227 6418 Size: 219 2228 125 277 736 1135 681 191 Cuts at: 6418 826 Size: 1471 Fragments arranged by size: 2228 1471 1135 736 681 277 219 191 125 SfoI GGC'GCC Cuts at: 2051 2051 Size: 7063 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7063 SmaI CCC'GGG Cuts at: 1872 2113 4390 1872 Size: 241 2277 4545 Fragments arranged by size: 4545 2277 241 SmlI C'TyrA_G Cuts at: 820 1017 1943 2491 2557 3222 3390 5167 6068 Size: 197 926 548 66 665 168 1777 901 Cuts at: 6068 6330 820 Size: 262 1553 Fragments arranged by size: 1777 1553 926 901 665 548 262 197 168 66 SnaBI TAC'GTA Cuts at: 493 2543 4854 493 Size: 2050 2311 2702 Fragments arranged by size: 2702 2311 2050 SpeI A'CTAG_T Cuts at: 152 152 Size: 7063 SphI G_CATG'C Cuts at: 1132 4421 1132 Size: 3289 3774 SspI AAT'ATT Cuts at: 5 52 2511 2613 3868 4987 5 Size: 47 2459 102 1255 1119 2081 Fragments arranged by size: 2459 2081 1255 1119 102 47 StuI AGG'CCT Cuts at: 1517 3505 1517 Size: 1988 5075 StyI C'CwwG_G Cuts at: 513 1065 4502 513 Size: 552 3437 3074 Fragments arranged by size: 3437 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 4189 5090 5107 5260 5445 5890 Size: 1255 741 1868 901 17 153 185 445 Cuts at: 5890 325 Size: 1498 Fragments arranged by size: 1868 1498 1255 901 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2825 3003 5309 Size: 274 80 33 51 1443 848 178 2306 Cuts at: 5309 7021 96 Size: 1712 138 Fragments arranged by size: 2306 1712 1443 848 274 178 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7023 7055 7023 Size: 32 7031 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7063 XbaI T'CTAG_A Cuts at: 4399 4399 Size: 7063 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2907 3061 2907 Size: 154 6909 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7063 XmaI C'CCGG_G Cuts at: 1870 2111 4388 1870 Size: 241 2277 4545 Fragments arranged by size: 4545 2277 241 XmnI GAAnn'nnTTC Cuts at: 2666 2952 5192 2666 Size: 286 2240 4537 Fragments arranged by size: 4537 2240 286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2242 4871 276 Size: 53 83 186 936 708 2629 2468 Fragments arranged by size: 2629 2468 936 708 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI