Restriction map for
pFN21AE5714
(Linear) (Six-base) MAPSORT of: KIEE5714.seq Check: 6043 from: 1 to: 2358
KIEE5714.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AatII G_ACGT'C
Cuts at: 0 239 2358
Size: 239 2119
AccI GT'mk_AC
Cuts at: 0 1610 2358
Size: 1610 748
Acc65I G'GTAC_C
Cuts at: 0 572 2358
Size: 572 1786
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 2358
Size: 962 1396
AflII C'TTAA_G
Cuts at: 0 552 2358
Size: 552 1806
AflIII A'CryG_T
Cuts at: 0 179 810 2358
Size: 179 631 1548
Fragments arranged by size:
1548 631 179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 87 2358
Size: 87 2271
AlwNI CAG_nnn'CTG
Cuts at: 0 324 2305 2358
Size: 324 1981 53
Fragments arranged by size:
1981 324 53
ApaI G_GGCC'C
Cuts at: 0 101 220 2358
Size: 101 119 2138
Fragments arranged by size:
2138 119 101
ApaLI G'TGCA_C
Cuts at: 0 2008 2358
Size: 2008 350
ApoI r'AATT_y
Cuts at: 0 295 314 875 1157 1487 1663 2058 2358
Size: 295 19 561 282 330 176 395 300
Fragments arranged by size:
561 395 330 300 295 282 176 19
AvaI C'yCGr_G
Cuts at: 0 106 2358
Size: 106 2252
BamHI G'GATC_C
Cuts at: 0 1506 2358
Size: 1506 852
BanI G'GyrC_C
Cuts at: 0 44 572 2134 2358
Size: 44 528 1562 224
Fragments arranged by size:
1562 528 224 44
BanII G_rGCy'C
Cuts at: 0 30 101 220 1805 2358
Size: 30 71 119 1585 553
Fragments arranged by size:
1585 553 119 71 30
BbeI G_GCGC'C
Cuts at: 0 48 2358
Size: 48 2310
BbsI GAAGACnn'nnnn_
Cuts at: 0 1519 2358
Size: 1519 839
BglI GCCn_nnn'nGGC
Cuts at: 0 53 2358
Size: 53 2305
BglII A'GATC_T
Cuts at: 0 716 1467 1968 2358
Size: 716 751 501 390
Fragments arranged by size:
751 716 501 390
BlpI GC'TnA_GC
Cuts at: 0 1541 1921 2358
Size: 1541 380 437
Fragments arranged by size:
1541 437 380
Bme1580I G_kGCm'C
Cuts at: 0 18 101 197 220 2012 2358
Size: 18 83 96 23 1792 346
Fragments arranged by size:
1792 346 96 83 23 18
BmgBI CAC'GTC
Cuts at: 0 182 2358
Size: 182 2176
BmrI ACTGGGnnnn_n'
Cuts at: 0 334 1331 2358
Size: 334 997 1027
Fragments arranged by size:
1027 997 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1957 2216 2358
Size: 698 1259 259 142
Fragments arranged by size:
1259 698 259 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 1202 1370 2358
Size: 507 695 168 988
Fragments arranged by size:
988 695 507 168
BsaAI yAC'GTr
Cuts at: 0 538 2358
Size: 538 1820
BsaBI GATnn'nnATC
Cuts at: 0 1170 2358
Size: 1170 1188
BsaHI Gr'CG_yC
Cuts at: 0 45 236 2358
Size: 45 191 2122
Fragments arranged by size:
2122 191 45
BsaWI w'CCGG_w
Cuts at: 0 157 2358
Size: 157 2201
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 982 1012 2358
Size: 982 30 1346
Fragments arranged by size:
1346 982 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 135 138 2215 2358
Size: 135 3 2077 143
Fragments arranged by size:
2077 143 135 3
BseYI C'CCAG_C
Cuts at: 0 220 2170 2358
Size: 220 1950 188
Fragments arranged by size:
1950 220 188
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 855 1707 2233 2358
Size: 473 382 852 526 125
Fragments arranged by size:
852 526 473 382 125
BsiHKAI G_wGCw'C
Cuts at: 0 924 2012 2358
Size: 924 1088 346
Fragments arranged by size:
1088 924 346
BsmBI CGTCTCn'nnnn_
Cuts at: 0 358 2035 2358
Size: 358 1677 323
Fragments arranged by size:
1677 358 323
Bsp1286I G_dGCh'C
Cuts at: 0 18 30 101 197 220 924 1805 2012
Size: 18 12 71 96 23 704 881 207
Cuts at: 2012 2358
Size: 346
Fragments arranged by size:
881 704 346 207 96 71 23 18
12
BspHI T'CATG_A
Cuts at: 0 1461 2358
Size: 1461 897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 975 2358
Size: 975 1383
BsrGI T'GTAC_A
Cuts at: 0 820 998 2358
Size: 820 178 1360
Fragments arranged by size:
1360 820 178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 2358
Size: 460 1898
BstBI TT'CG_AA
Cuts at: 0 722 2358
Size: 722 1636
BstXI CCAn_nnnn'nTGG
Cuts at: 0 569 2358
Size: 569 1789
BstYI r'GATC_y
Cuts at: 0 716 891 1467 1506 1878 1905 1968 2358
Size: 716 175 576 39 372 27 63 390
Fragments arranged by size:
716 576 390 372 175 63 39 27
Bsu36I CC'TnA_GG
Cuts at: 0 212 2358
Size: 212 2146
BtsI GCAGTG_nn'
Cuts at: 0 843 2358
Size: 843 1515
DraI TTT'AAA
Cuts at: 0 1434 1662 2358
Size: 1434 228 696
Fragments arranged by size:
1434 696 228
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 1108 2358
Size: 235 873 1250
Fragments arranged by size:
1250 873 235
EaeI y'GGCC_r
Cuts at: 0 54 2358
Size: 54 2304
EarI CTCTTCn'nnn_
Cuts at: 0 361 764 938 1489 2358
Size: 361 403 174 551 869
Fragments arranged by size:
869 551 403 361 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 962 1957 2216 2358
Size: 698 264 995 259 142
Fragments arranged by size:
995 698 264 259 142
EcoO109I rG'GnC_Cy
Cuts at: 0 98 216 217 619 2358
Size: 98 118 1 402 1739
Fragments arranged by size:
1739 402 118 98 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 303 2358
Size: 303 2055
HaeII r_GCGC'y
Cuts at: 0 48 227 2358
Size: 48 179 2131
Fragments arranged by size:
2131 179 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 876 908 2358
Size: 876 32 1450
Fragments arranged by size:
1450 876 32
HindIII A'AGCT_T
Cuts at: 0 365 375 2358
Size: 365 10 1983
Fragments arranged by size:
1983 365 10
KasI G'GCGC_C
Cuts at: 0 44 2358
Size: 44 2314
KpnI G_GTAC'C
Cuts at: 0 576 2358
Size: 576 1782
MfeI C'AATT_G
Cuts at: 0 1277 2358
Size: 1277 1081
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1558 2358
Size: 497 1061 800
Fragments arranged by size:
1061 800 497
MscI TGG'CCA
Cuts at: 0 56 2358
Size: 56 2302
MslI CAynn'nnrTG
Cuts at: 0 464 956 1054 2358
Size: 464 492 98 1304
Fragments arranged by size:
1304 492 464 98
MspA1I CmG'CkG
Cuts at: 0 597 1177 1522 2358
Size: 597 580 345 836
Fragments arranged by size:
836 597 580 345
NarI GG'CG_CC
Cuts at: 0 45 2358
Size: 45 2313
NspI r_CATG'y
Cuts at: 0 496 814 2241 2358
Size: 496 318 1427 117
Fragments arranged by size:
1427 496 318 117
PciI A'CATG_T
Cuts at: 0 810 2358
Size: 810 1548
PflMI CCAn_nnn'nTGG
Cuts at: 0 905 2358
Size: 905 1453
PsiI TTA'TAA
Cuts at: 0 1330 2358
Size: 1330 1028
PspOMI G'GGCC_C
Cuts at: 0 97 216 2358
Size: 97 119 2142
Fragments arranged by size:
2142 119 97
PstI C_TGCA'G
Cuts at: 0 1397 1674 2358
Size: 1397 277 684
Fragments arranged by size:
1397 684 277
PvuII CAG'CTG
Cuts at: 0 1177 1522 2358
Size: 1177 345 836
Fragments arranged by size:
1177 836 345
SapI GCTCTTCn'nnn_
Cuts at: 0 764 2358
Size: 764 1594
SexAI A'CCwGG_T
Cuts at: 0 980 2358
Size: 980 1378
SfcI C'TryA_G
Cuts at: 0 1268 1393 1670 2358
Size: 1268 125 277 688
Fragments arranged by size:
1268 688 277 125
SfoI GGC'GCC
Cuts at: 0 46 2358
Size: 46 2312
SmaI CCC'GGG
Cuts at: 0 108 2358
Size: 108 2250
SmlI C'TyrA_G
Cuts at: 0 486 552 1217 1385 2358
Size: 486 66 665 168 973
Fragments arranged by size:
973 665 486 168 66
SnaBI TAC'GTA
Cuts at: 0 538 2358
Size: 538 1820
SspI AAT'ATT
Cuts at: 0 506 608 1863 2358
Size: 506 102 1255 495
Fragments arranged by size:
1255 506 495 102
StuI AGG'CCT
Cuts at: 0 1500 2358
Size: 1500 858
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 2184 2358
Size: 316 1868 174
Fragments arranged by size:
1868 316 174
TatI w'GTAC_w
Cuts at: 0 820 998 2358
Size: 820 178 1360
Fragments arranged by size:
1360 820 178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 902 1056 2358
Size: 902 154 1302
Fragments arranged by size:
1302 902 154
XmaI C'CCGG_G
Cuts at: 0 106 2358
Size: 106 2252
XmnI GAAnn'nnTTC
Cuts at: 0 661 947 2358
Size: 661 286 1411
Fragments arranged by size:
1411 661 286
ZraI GAC'GTC
Cuts at: 0 237 2358
Size: 237 2121
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrGI BstAPI
BstBI BstXI BstYI Bsu36I BtsI DraI DrdI EaeI
EarI Eco57MI EcoO109I FalI HaeII Hin4I HindIII KasI
KpnI MfeI MmeI MscI MslI MspA1I NarI NspI
PciI PflMI PsiI PspOMI PstI PvuII SapI SexAI
SfcI SfoI SmaI SmlI SnaBI SspI StuI TaqII
TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AhdI AleI AloI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI
BfrBI BmtI BplI Bpu10I BsaI BsiEI BsiWI BsmI
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstEII BstZ17I
BtgI BtgZI ClaI CspCI DraIII EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SpeI SphI
SrfI StyI SwaI TstI Tth111I XbaI XhoI