################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRII EcoRV FauI FbaI Fnu4HI FseI HaeII HgaI Hin1I HindIII HinfI HpaI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI TfiI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AfaI 397 GTAC GT^AC AlwI 330 GGATC GGATC(4/5) AlwNI 308 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AviII 74 TGCGCA TGC^GCA BcnI 35 CCGGG CC^[GC]GG BfaI 355 CTAG C^TAG BpmI 26 CTGGAG CTGGAG(16/14) BsmI 278 GCATTC GAATGC(1/-1) Bsp1407I 396 TGTACA T^GTACA BspMI 385 GCAGGT ACCTGC(4/8) BsrDI 418 GCAATG GCAATG(2/0) BsrGI 396 TGTACA T^GTACA BssKI 35 CCGGG ^CC[ACGT]GG BstEII 289 GGTTACC G^GT[ACGT]ACC BstF5I 237 GGATG GGATG(2/0) BstPI 289 GGTTACC G^GT[ACGT]ACC BstYI 330 GGATCT [GA]^GATC[CT] CfoI 75 GCGC GCG^C DpnII 331 GATC ^GATC EcoO65I 289 GGTTACC G^GT[ACGT]ACC EcoT14I 161 CCAAGG C^C[AT][AT]GG EcoT22I 276 ATGCAT ATGCA^T FokI 237 GGATG GGATG(9/13) FspI 74 TGCGCA TGC^GCA HaeIII 69 GGCC GG^CC HapII 35 CCGG C^CGG HhaI 75 GCGC GCG^C HinP1I 75 GCGC G^CGC HincII 129 GTCAAC GT[CT]^[GA]AC HindII 129 GTCAAC GT[CT]^[GA]AC HpaII 35 CCGG C^CGG MaeI 355 CTAG C^TAG MaeIII 290 GTTAC ^GT[ACGT]AC MboI 331 GATC ^GATC MflI 330 GGATCT [GA]^GATC[CT] MslI 151 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 35 CCGG C^CGG MwoI 77 GCAAAAACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 35 CCGGG CC^[GC]GG NdeII 331 GATC ^GATC NsiI 276 ATGCAT ATGCA^T RsaI 397 GTAC GT^AC Sau3AI 331 GATC ^GATC ScrFI 35 CCGGG CC^[ACGT]GG SfaNI 31 GATGC GCATC(5/9) SfcI 437 CTGTAG C^T[GA][CT]AG SspBI 396 TGTACA T^GTACA SspI 247 AATATT AAT^ATT StyI 161 CCAAGG C^C[AT][AT]GG TspRI 61 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 330 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AspHI 63 GTGCTC G[AT]GC[AT]^C 94 GTGCTC BmyI 63 GTGCTC G[AGT]GC[ACT]^C 94 GTGCTC BsaJI 35 CCGGGG C^C[ACGT][ACGT]GG 161 CCAAGG BsiHKAI 63 GTGCTC G[AT]GC[AT]^C 94 GTGCTC Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C 94 GTGCTC MnlI 328 GAGG CCTC(7/6) 370 GAGG MseI 251 TTAA T^TAA 335 TTAA Tru9I 251 TTAA T^TAA 335 TTAA Tsp509I 12 AATT ^AATT 180 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### BsrI HphI MboII NlaIII