(Circular) (Six-base) MAPSORT of: pFN21AE3671.seq Check: 2015 from: 1 to: 7615 pFN21AE3671 7615 bp With 182 enzymes: SgfI * March 28, 2012 12:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5425 278 Size: 53 83 186 936 3889 2468 Fragments arranged by size: 3889 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2082 2398 4958 1937 Size: 145 316 2560 4594 Fragments arranged by size: 4594 2560 316 145 Acc65I G'GTAC_C Cuts at: 4936 4936 Size: 7615 AclI AA'CG_TT Cuts at: 1563 5742 6115 1563 Size: 4179 373 3063 Fragments arranged by size: 4179 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2760 2997 3899 5678 7062 7330 1632 Size: 1128 237 902 1779 1384 268 1917 Fragments arranged by size: 1917 1779 1384 1128 902 268 237 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7418 AflIII A'CryG_T Cuts at: 1203 6514 7491 1203 Size: 5311 977 1327 Fragments arranged by size: 5311 1327 977 AgeI A'CCGG_T Cuts at: 6446 6446 Size: 7615 AhdI GACnn_n'nnGTC Cuts at: 6344 6344 Size: 7615 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7615 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3477 4545 1184 Size: 2293 1068 4254 Fragments arranged by size: 4254 2293 1068 AlwNI CAG_nnn'CTG Cuts at: 4607 4867 5358 6930 4607 Size: 260 491 1572 5292 Fragments arranged by size: 5292 1572 491 260 ApaLI G'TGCA_C Cuts at: 2562 5672 6828 2562 Size: 3110 1156 3349 Fragments arranged by size: 3349 3110 1156 ApoI r'AATT_y Cuts at: 1493 2806 4153 4924 5150 5302 7411 1493 Size: 1313 1347 771 226 152 2109 1697 Fragments arranged by size: 2109 1697 1347 1313 771 226 152 AseI AT'TA_AT Cuts at: 160 1032 6169 160 Size: 872 5137 1606 Fragments arranged by size: 5137 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7615 AvaI C'yCGr_G Cuts at: 1870 1943 4940 1870 Size: 73 2997 4545 Fragments arranged by size: 4545 2997 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3109 3142 3558 3591 3109 Size: 33 416 33 7133 Fragments arranged by size: 7133 416 33 33 BamHI G'GATC_C Cuts at: 4945 5312 4945 Size: 367 7248 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4546 4936 6391 618 Size: 325 217 627 2759 390 1455 1842 Fragments arranged by size: 2759 1842 1455 627 390 325 217 BanII G_rGCy'C Cuts at: 1391 4934 1391 Size: 3543 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2766 2850 2941 4637 4767 4863 928 Size: 971 867 84 91 1696 130 96 3680 Fragments arranged by size: 3680 1696 971 867 130 96 91 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5806 5840 1903 Size: 34 3869 34 3678 Fragments arranged by size: 3869 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5507 6717 5507 Size: 1210 6405 BclI T'GATC_A Cuts at: 1548 1548 Size: 7615 BfrBI ATG'CAT Cuts at: 3887 4140 4522 3887 Size: 253 382 6980 Fragments arranged by size: 6980 382 253 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6226 136 Size: 107 122 71 631 605 4554 1525 Fragments arranged by size: 4554 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3146 4094 7610 3146 Size: 948 3516 3151 Fragments arranged by size: 3516 3151 948 BlpI GC'TnA_GC Cuts at: 5032 5032 Size: 7615 Bme1580I G_kGCm'C Cuts at: 1790 2566 5676 6832 1790 Size: 776 3110 1156 2573 Fragments arranged by size: 3110 2573 1156 776 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7615 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4744 5346 6304 Size: 416 53 83 399 24 3327 602 958 Cuts at: 6304 442 Size: 1753 Fragments arranged by size: 3327 1753 958 602 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7615 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3280 3280 Size: 7615 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6275 1239 Size: 5036 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3156 3876 5740 6605 6903 3156 Size: 720 1864 865 298 3868 Fragments arranged by size: 3868 1864 865 720 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2620 6278 882 Size: 617 1121 3658 2219 Fragments arranged by size: 3658 2219 1121 617 BsaAI yAC'GTr Cuts at: 493 1204 5406 493 Size: 711 4202 2702 Fragments arranged by size: 4202 2702 711 BsaBI GATnn'nnATC Cuts at: 3351 4099 7609 3351 Size: 748 3510 3357 Fragments arranged by size: 3510 3357 748 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5422 5804 6439 275 Size: 53 83 186 936 3889 382 635 1451 Fragments arranged by size: 3889 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6048 6446 6720 6867 7278 6048 Size: 398 274 147 411 6385 Fragments arranged by size: 6385 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7390 Fragments arranged by size: 7390 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3255 3869 4307 4449 1186 Size: 2069 614 438 142 4352 Fragments arranged by size: 4352 2069 614 438 142 BseYI C'CCAG_C Cuts at: 6818 7314 6818 Size: 496 7119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3630 4781 1163 Size: 573 1894 1151 3997 Fragments arranged by size: 3997 1894 1151 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5065 5826 6854 1540 Size: 275 186 3064 761 1028 2301 Fragments arranged by size: 3064 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2566 4393 5676 5761 6832 2566 Size: 1827 1283 85 1071 3349 Fragments arranged by size: 3349 1827 1283 1071 85 BsmI GAATG_Cn' Cuts at: 5126 5219 5126 Size: 93 7522 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2566 4393 4934 5676 5761 6832 1391 Size: 399 776 1827 541 742 85 1071 2174 Fragments arranged by size: 2174 1827 1071 776 742 541 399 85 BspEI T'CCGG_A Cuts at: 7278 7278 Size: 7615 BspHI T'CATG_A Cuts at: 5504 7234 5504 Size: 1730 5885 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 4970 844 Size: 1177 2949 3489 Fragments arranged by size: 3489 2949 1177 BsrBI CCG'CTC Cuts at: 1394 1823 5502 1394 Size: 429 3679 3507 Fragments arranged by size: 3679 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2194 6110 6284 65 Size: 1183 946 3916 174 1396 Fragments arranged by size: 3916 1396 1183 946 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6259 6446 1700 Size: 110 4449 187 2869 Fragments arranged by size: 4449 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2148 4081 96 Size: 2052 1933 3630 Fragments arranged by size: 3630 2052 1933 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7615 BssSI C'ACGA_G Cuts at: 5675 6687 5675 Size: 1012 6603 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7615 BstBI TT'CG_AA Cuts at: 6419 6419 Size: 7615 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7615 BstYI r'GATC_y Cuts at: 1972 3146 3329 3697 4094 4945 5312 5697 5714 Size: 1174 183 368 397 851 367 385 17 Cuts at: 5714 7166 7252 7264 7610 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1174 851 397 385 368 367 346 183 86 17 12 BstZ17I GTA'TAC Cuts at: 2399 2399 Size: 7615 Bsu36I CC'TnA_GG Cuts at: 4443 4443 Size: 7615 BtgI C'CryG_G Cuts at: 513 1065 2004 3740 513 Size: 552 939 1736 4388 Fragments arranged by size: 4388 1736 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6876 Fragments arranged by size: 6876 683 56 BtsI GCAGTG_nn' Cuts at: 5131 5925 5945 5131 Size: 794 20 6801 Fragments arranged by size: 6801 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7615 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7580 DraI TTT'AAA Cuts at: 2041 3304 4260 4919 2041 Size: 1263 956 659 4737 Fragments arranged by size: 4737 1263 956 659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4246 6622 809 Size: 507 2930 2376 1802 Fragments arranged by size: 2930 2376 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5062 5951 7580 Size: 54 1425 50 275 18 3232 889 1629 Cuts at: 7580 8 Size: 43 Fragments arranged by size: 3232 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5062 1537 Size: 275 3250 4090 Fragments arranged by size: 4090 3250 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3073 3249 4362 4627 5545 1353 Size: 1720 176 1113 265 918 3423 Fragments arranged by size: 3423 1720 1113 918 265 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6202 6576 6722 387 Size: 1064 138 4613 374 146 1280 Fragments arranged by size: 4613 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2760 2997 3899 5678 6275 7062 7330 Size: 393 1128 237 902 1779 597 787 268 Cuts at: 7330 1239 Size: 1524 Fragments arranged by size: 1779 1524 1128 902 787 597 393 268 237 EcoO109I rG'GnC_Cy Cuts at: 2410 2722 4746 2410 Size: 312 2024 5279 Fragments arranged by size: 5279 2024 312 EcoRI G'AATT_C Cuts at: 4153 4924 5302 4153 Size: 771 378 6466 Fragments arranged by size: 6466 771 378 EcoRV GAT'ATC Cuts at: 3921 3921 Size: 7615 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2790 3929 4661 2790 Size: 1139 732 5744 Fragments arranged by size: 5744 1139 732 FspI TGC'GCA Cuts at: 6121 6121 Size: 7615 HaeII r_GCGC'y Cuts at: 1678 6762 1678 Size: 5084 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2365 2397 2573 2605 3872 Size: 32 135 32 793 32 176 32 1267 Cuts at: 3872 3904 4484 4516 6256 6288 6330 6362 1373 Size: 32 580 32 1740 32 42 32 2626 Fragments arranged by size: 2626 1740 1267 793 580 176 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4710 4959 5205 669 Size: 988 281 2772 249 246 3079 Fragments arranged by size: 3079 2772 988 281 249 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7615 HpaI GTT'AAC Cuts at: 4710 5205 4710 Size: 495 7120 KpnI G_GTAC'C Cuts at: 4940 4940 Size: 7615 MfeI C'AATT_G Cuts at: 2201 2850 5214 2201 Size: 649 2364 4602 Fragments arranged by size: 4602 2364 649 MluI A'CGCG_T Cuts at: 7491 7491 Size: 7615 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2026 3697 4562 4922 6729 6913 1305 Size: 721 1671 865 360 1807 184 2007 Fragments arranged by size: 2007 1807 1671 865 721 360 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5793 Fragments arranged by size: 5793 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2372 2391 4145 4226 4320 5573 5932 Size: 648 1206 19 1754 81 94 1253 359 Cuts at: 5932 6091 518 Size: 159 2042 Fragments arranged by size: 2042 1754 1253 1206 648 359 159 94 81 19 MspA1I CmG'CkG Cuts at: 1606 5031 5708 6856 7101 1606 Size: 3425 677 1148 245 2120 Fragments arranged by size: 3425 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3740 513 Size: 552 939 1736 4388 Fragments arranged by size: 4388 1736 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7615 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7615 NotI GC'GGCC_GC Cuts at: 5062 5062 Size: 7615 NsiI A_TGCA'T Cuts at: 3889 4142 4524 3889 Size: 253 382 6980 Fragments arranged by size: 6980 382 253 NspI r_CATG'y Cuts at: 1132 1229 2867 4973 6518 1132 Size: 97 1638 2106 1545 2229 Fragments arranged by size: 2229 2106 1638 1545 97 PasI CC'CwG_GG Cuts at: 1348 2593 1348 Size: 1245 6370 PciI A'CATG_T Cuts at: 6514 6514 Size: 7615 PflMI CCAn_nnn'nTGG Cuts at: 1108 3962 1108 Size: 2854 4761 PfoI T'CCnGG_A Cuts at: 3379 3379 Size: 7615 PmeI GTTT'AAAC Cuts at: 4919 4919 Size: 7615 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5684 5716 7197 7229 5684 Size: 32 1481 32 6070 Fragments arranged by size: 6070 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2722 4746 2410 Size: 312 2024 5279 Fragments arranged by size: 5279 2024 312 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7615 PsiI TTA'TAA Cuts at: 2232 5185 2232 Size: 2953 4662 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4420 4452 4420 Size: 32 7583 PstI C_TGCA'G Cuts at: 830 4967 830 Size: 4137 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7615 SalI G'TCGA_C Cuts at: 1936 4957 1936 Size: 3021 4594 SanDI GG'GwC_CC Cuts at: 4746 4746 Size: 7615 SbfI CC_TGCA'GG Cuts at: 4967 4967 Size: 7615 ScaI AGT'ACT Cuts at: 4432 5863 7575 4432 Size: 1431 1712 4472 Fragments arranged by size: 4472 1712 1431 SexAI A'CCwGG_T Cuts at: 4423 4423 Size: 7615 SfcI C'TryA_G Cuts at: 826 1045 2083 4599 4963 6098 6779 6970 826 Size: 219 1038 2516 364 1135 681 191 1471 Fragments arranged by size: 2516 1471 1135 1038 681 364 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7615 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7615 SmaI CCC'GGG Cuts at: 1872 4942 1872 Size: 3070 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3171 3891 5719 6620 6882 820 Size: 197 926 1228 720 1828 901 262 1553 Fragments arranged by size: 1828 1553 1228 926 901 720 262 197 SnaBI TAC'GTA Cuts at: 493 5406 493 Size: 4913 2702 SpeI A'CTAG_T Cuts at: 152 4433 4712 152 Size: 4281 279 3055 Fragments arranged by size: 4281 3055 279 SphI G_CATG'C Cuts at: 1132 2867 4973 1132 Size: 1735 2106 3774 Fragments arranged by size: 3774 2106 1735 SspI AAT'ATT Cuts at: 5 52 5539 5 Size: 47 5487 2081 Fragments arranged by size: 5487 2081 47 StuI AGG'CCT Cuts at: 1517 4448 1517 Size: 2931 4684 StyI C'CwwG_G Cuts at: 513 1065 2004 2718 3740 5054 513 Size: 552 939 714 1022 1314 3074 Fragments arranged by size: 3074 1314 1022 939 714 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3283 5642 5659 5812 5997 6442 325 Size: 1255 1703 2359 17 153 185 445 1498 Fragments arranged by size: 2359 1703 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 4081 4430 Size: 274 80 33 51 1443 171 1933 349 Cuts at: 4430 5861 7573 96 Size: 1431 1712 138 Fragments arranged by size: 1933 1712 1443 1431 349 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7575 7607 7575 Size: 32 7583 Tth111I GACn'n_nGTC Cuts at: 1316 2648 4587 1316 Size: 1332 1939 4344 Fragments arranged by size: 4344 1939 1332 XbaI T'CTAG_A Cuts at: 4951 4951 Size: 7615 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3829 4047 3829 Size: 218 7397 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7615 XmaI C'CCGG_G Cuts at: 1870 4940 1870 Size: 3070 4545 XmnI GAAnn'nnTTC Cuts at: 2324 2809 3912 5744 2324 Size: 485 1103 1832 4195 Fragments arranged by size: 4195 1832 1103 485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5423 276 Size: 53 83 186 936 3889 2468 Fragments arranged by size: 3889 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI