Restriction map for
pFN21AE5704
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsgI BsiHKAI BsiWI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BssHII BstBI BstEII BstPI
BstXI Bsu36I CelII CfoI ClaI
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII KasI KpnI KspI MaeII
MamI MluI MluNI MscI NarI
NcoI NdeI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 132 CGCG CG^CG
AccIII 903 TCCGGA T^CCGGA
AlwNI 1796 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 3 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 689 GAGCCC G[GA]GC[CT]^C
BbvCI 1282 CCTCAGC CCTCAGC(-5/-2)
BciVI 1596 GTATCC GTATCC(6/5)
BclI 701 TGATCA T^GATCA
BpmI 1312 CTCCAG CTGGAG(16/14)
BsaWI 903 TCCGGA [AT]^CCGG[AT]
BseAI 903 TCCGGA T^CCGGA
BsmBI 1664 CGTCTC CGTCTC(1/5)
BsmI 1560 GCATTC GAATGC(1/-1)
BspEI 903 TCCGGA T^CCGGA
BsrBI 50 GAGCGG CCGCTC(-3/-3)
BsrFI 88 GCCGGC [GA]^CCGG[CT]
BssSI 199 CTCGTG CACGAG(-5/-1)
Bst1107I 669 GTATAC GTA^TAC
BstAPI 1530 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 132 CGCG CG^CG
BstZ17I 669 GTATAC GTA^TAC
Cfr10I 88 GCCGGC [GA]^CCGG[CT]
DraI 946 TTTAAA TTT^AAA
DraII 1275 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 113 CCGTGG C^C[GA][CT]GG
EagI 138 CGGCCG C^GGCCG
EclXI 138 CGGCCG C^GGCCG
Eco52I 138 CGGCCG C^GGCCG
EcoO109I 1275 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1207 GAATTC G^AATTC
EcoRV 742 GATATC GAT^ATC
EcoT14I 734 CCAAGG C^C[AT][AT]GG
EcoT22I 1558 ATGCAT ATGCA^T
FbaI 701 TGATCA T^GATCA
HincII 1902 GTTAAC GT[CT]^[GA]AC
HindII 1902 GTTAAC GT[CT]^[GA]AC
HpaI 1902 GTTAAC GTT^AAC
MfeI 1100 CAATTG C^AATTG
MroI 903 TCCGGA T^CCGGA
MslI 752 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1100 CAATTG C^AATTG
MvnI 132 CGCG CG^CG
NaeI 88 GCCGGC GCC^GGC
NgoMIV 88 GCCGGC G^CCGGC
NheI 1647 GCTAGC G^CTAGC
NsiI 1558 ATGCAT ATGCA^T
PpuMI 1275 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1037 CAGCTG CAG^CTG
SapI 1006 GCTCTTC GCTCTTC(1/4)
ScaI 1308 AGTACT AGT^ACT
SexAI 146 ACCAGGT A^CC[AT]GGT
SmlI 1703 CTCAAG C^T[CT][GA]AG
StyI 734 CCAAGG C^C[AT][AT]GG
SwaI 945 ATTTAAAT ATTT^AAAT
TaqI 399 TCGA T^CGA
TthHB8I 399 TCGA T^CGA
XmnI 3 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 669 GTATAC GT^[AC][GT]AC
761 GTCTAC
AlwI 901 GATCC GGATC(4/5)
537 GGATC
BseRI 1280 CTCCTC GAGGAG(10/8)
1773 CTCCTC
BsiEI 134 CGGTCG CG[GA][CT]^CG
138 CGGCCG
BsmAI 973 GTCTC GTCTC(1/5)
1665 GTCTC
BsmFI 1275 GGGAC GGGAC(10/14)
1956 GGGAC
BsrDI 871 CATTGC GCAATG(2/0)
1815 CATTGC
BstYI 537 GGATCT [GA]^GATC[CT]
900 AGATCC
EaeI 138 CGGCCG [CT]^GGCC[GA]
170 TGGCCG
MflI 537 GGATCT [GA]^GATC[CT]
900 AGATCC
MspA1I 39 CAGCGG C[AC]G^C[GT]G
1037 CAGCTG
NspI 254 ACATGC [GA]CATG^[CT]
1515 ACATGC
XhoII 537 GGATCT [GA]^GATC[CT]
900 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BcnI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI