(Circular) (Six-base) MAPSORT of: pFN21AE5704.seq Check: 1512 from: 1 to: 6691 pFN21AE5704 6691 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4501 278 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2675 2767 4034 1937 Size: 738 92 1267 4594 Fragments arranged by size: 4594 1267 738 92 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2567 2991 4754 6138 6406 1632 Size: 935 424 1763 1384 268 1917 Fragments arranged by size: 1917 1763 1384 935 424 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6494 AflIII A'CryG_T Cuts at: 1203 5590 6567 1203 Size: 4387 977 1327 Fragments arranged by size: 4387 1327 977 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 5420 5420 Size: 6691 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6691 AlwNI CAG_nnn'CTG Cuts at: 3806 4434 6006 3806 Size: 628 1572 4491 Fragments arranged by size: 4491 1572 628 ApaLI G'TGCA_C Cuts at: 4748 5904 4748 Size: 1156 5535 ApoI r'AATT_y Cuts at: 1493 2618 2954 3212 3264 3826 3935 4000 4226 Size: 1125 336 258 52 562 109 65 226 Cuts at: 4226 4378 6487 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1125 562 336 258 226 152 109 65 52 AseI AT'TA_AT Cuts at: 160 1032 5245 160 Size: 872 4213 1606 Fragments arranged by size: 4213 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 4016 1870 Size: 73 2073 4545 Fragments arranged by size: 4545 2073 73 BamHI G'GATC_C Cuts at: 4021 4388 4021 Size: 367 6324 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4012 5467 618 Size: 325 217 627 2225 1455 1842 Fragments arranged by size: 2225 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2698 4010 1391 Size: 1307 1312 4072 Fragments arranged by size: 4072 1312 1307 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5720 BbvCI CC'TCA_GC Cuts at: 3288 3288 Size: 6691 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4882 4916 1903 Size: 34 2945 34 3678 Fragments arranged by size: 3678 2945 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3612 4583 5793 3612 Size: 971 1210 4510 Fragments arranged by size: 4510 1210 971 BclI T'GATC_A Cuts at: 1548 2706 1548 Size: 1158 5533 BfrBI ATG'CAT Cuts at: 3565 3565 Size: 6691 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6686 6686 Size: 6691 BlpI GC'TnA_GC Cuts at: 4108 4108 Size: 6691 Bme1580I G_kGCm'C Cuts at: 1790 2758 3241 4752 5908 1790 Size: 968 483 1511 1156 2573 Fragments arranged by size: 2573 1511 1156 968 483 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6691 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2768 3150 4422 Size: 416 53 83 399 24 1351 382 1272 Cuts at: 4422 5380 442 Size: 958 1753 Fragments arranged by size: 1753 1351 1272 958 416 399 382 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3656 1056 Size: 2600 4091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3302 5351 1239 Size: 2063 2049 2579 Fragments arranged by size: 2579 2063 2049 Bpu10I CC'TnA_GC Cuts at: 3288 3756 3288 Size: 468 6223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3693 4816 5681 5979 3693 Size: 1123 865 298 4405 Fragments arranged by size: 4405 1123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5354 882 Size: 617 3855 2219 Fragments arranged by size: 3855 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4482 493 Size: 711 3278 2702 Fragments arranged by size: 3278 2702 711 BsaBI GATnn'nnATC Cuts at: 6685 6685 Size: 6691 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4498 4880 5515 275 Size: 53 83 186 936 2965 382 635 1451 Fragments arranged by size: 2965 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2908 5124 5522 5796 5943 6354 2908 Size: 2216 398 274 147 411 3245 Fragments arranged by size: 3245 2216 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2634 2664 3136 3166 3339 Size: 30 165 30 1231 30 472 30 173 Cuts at: 3339 3369 1178 Size: 30 4500 Fragments arranged by size: 4500 1231 472 173 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3276 3769 1186 Size: 2090 493 4108 Fragments arranged by size: 4108 2090 493 BseYI C'CCAG_C Cuts at: 5894 6390 5894 Size: 496 6195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6118 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 2146 4141 4902 5930 1540 Size: 275 186 141 4 1995 761 1028 2301 Fragments arranged by size: 2301 1995 1028 761 275 186 141 4 BsiHKAI G_wGCw'C Cuts at: 4752 4837 5908 4752 Size: 85 1071 5535 Fragments arranged by size: 5535 1071 85 BsmI GAATG_Cn' Cuts at: 3565 4202 4295 3565 Size: 637 93 5961 Fragments arranged by size: 5961 637 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3675 3675 Size: 6691 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2698 2758 3241 4010 4752 4837 5908 Size: 399 908 60 483 769 742 85 1071 Cuts at: 5908 1391 Size: 2174 Fragments arranged by size: 2174 1071 908 769 742 483 399 85 60 BspEI T'CCGG_A Cuts at: 2908 6354 2908 Size: 3446 3245 BspHI T'CATG_A Cuts at: 4580 6310 4580 Size: 1730 4961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4046 844 Size: 3202 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2057 4578 1394 Size: 429 234 2521 3507 Fragments arranged by size: 3507 2521 429 234 BsrDI GCAATG_nn' Cuts at: 65 1248 2875 3819 5186 5360 65 Size: 1183 1627 944 1367 174 1396 Fragments arranged by size: 1627 1396 1367 1183 944 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 5335 5522 1700 Size: 110 283 3242 187 2869 Fragments arranged by size: 3242 2869 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6691 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 2204 4751 5763 2204 Size: 2547 1012 3132 Fragments arranged by size: 3132 2547 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3541 794 Size: 2747 3944 BstBI TT'CG_AA Cuts at: 5495 5495 Size: 6691 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6691 BstYI r'GATC_y Cuts at: 1972 2542 2905 4021 4388 4773 4790 6242 6328 Size: 570 363 1116 367 385 17 1452 86 Cuts at: 6328 6340 6686 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1116 570 385 367 363 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2676 2676 Size: 6691 BtgI C'CryG_G Cuts at: 513 1065 2118 513 Size: 552 1053 5086 Fragments arranged by size: 5086 1053 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5952 Fragments arranged by size: 5952 683 56 BtsI GCAGTG_nn' Cuts at: 2611 4207 5001 5021 2611 Size: 1596 794 20 4281 Fragments arranged by size: 4281 1596 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6656 DraI TTT'AAA Cuts at: 2953 3995 2953 Size: 1042 5649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5698 809 Size: 507 4382 1802 Fragments arranged by size: 4382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2175 4138 Size: 54 1425 50 275 18 313 32 1963 Cuts at: 4138 5027 6656 8 Size: 889 1629 43 Fragments arranged by size: 1963 1629 1425 889 313 275 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2143 4138 1537 Size: 275 331 1995 4090 Fragments arranged by size: 4090 1995 331 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 2799 3018 3909 4621 1353 Size: 807 639 219 891 712 3423 Fragments arranged by size: 3423 891 807 712 639 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2067 5278 5652 5798 387 Size: 1064 138 478 3211 374 146 1280 Fragments arranged by size: 3211 1280 1064 478 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2567 2991 3302 4754 5351 6138 6406 Size: 393 935 424 311 1452 597 787 268 Cuts at: 6406 1239 Size: 1524 Fragments arranged by size: 1524 1452 935 787 597 424 393 311 268 EcoO109I rG'GnC_Cy Cuts at: 3281 3281 Size: 6691 EcoRI G'AATT_C Cuts at: 3212 4000 4378 3212 Size: 788 378 5525 Fragments arranged by size: 5525 788 378 EcoRV GAT'ATC Cuts at: 2749 2749 Size: 6691 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3982 3982 Size: 6691 FspI TGC'GCA Cuts at: 5197 5197 Size: 6691 HaeII r_GCGC'y Cuts at: 1678 5838 1678 Size: 4160 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2754 2786 3640 3672 3884 Size: 32 135 32 1182 32 854 32 212 Cuts at: 3884 3916 5332 5364 5406 5438 1373 Size: 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1416 1182 854 212 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3909 4035 4281 669 Size: 988 281 1971 126 246 3079 Fragments arranged by size: 3079 1971 988 281 246 126 HindIII A'AGCT_T Cuts at: 748 748 Size: 6691 HpaI GTT'AAC Cuts at: 3909 4281 3909 Size: 372 6319 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 3105 4290 3105 Size: 1185 5506 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2013 3006 3742 5805 5989 1305 Size: 708 993 736 2063 184 2007 Fragments arranged by size: 2063 2007 993 736 708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4869 Fragments arranged by size: 4869 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2761 4649 5008 5167 518 Size: 648 1595 1888 359 159 2042 Fragments arranged by size: 2042 1888 1595 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 3044 4107 4784 5932 6177 1606 Size: 440 998 1063 677 1148 245 2120 Fragments arranged by size: 2120 1148 1063 998 677 440 245 NaeI GCC'GGC Cuts at: 2095 2095 Size: 6691 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6139 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NgoMIV G'CCGG_C Cuts at: 2093 2093 Size: 6691 NheI G'CTAG_C Cuts at: 1052 3652 1052 Size: 2600 4091 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NsiI A_TGCA'T Cuts at: 3567 3567 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 2263 3524 4049 5594 1132 Size: 97 1034 1261 525 1545 2229 Fragments arranged by size: 2229 1545 1261 1034 525 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6691 PciI A'CATG_T Cuts at: 5590 5590 Size: 6691 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6691 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4760 4792 6273 6305 4760 Size: 32 1481 32 5146 Fragments arranged by size: 5146 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3281 3281 Size: 6691 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 4261 4261 Size: 6691 PstI C_TGCA'G Cuts at: 830 4043 830 Size: 3213 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 PvuII CAG'CTG Cuts at: 3044 3044 Size: 6691 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SapI GCTCTTCn'nnn_ Cuts at: 3018 3018 Size: 6691 SbfI CC_TGCA'GG Cuts at: 4043 4043 Size: 6691 ScaI AGT'ACT Cuts at: 3315 4939 6651 3315 Size: 1624 1712 3355 Fragments arranged by size: 3355 1712 1624 SexAI A'CCwGG_T Cuts at: 2151 2151 Size: 6691 SfcI C'TryA_G Cuts at: 826 1045 2441 3326 3784 4039 5174 5855 6046 Size: 219 1396 885 458 255 1135 681 191 Cuts at: 6046 826 Size: 1471 Fragments arranged by size: 1471 1396 1135 885 681 458 255 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 4018 1872 Size: 2146 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3708 4795 5696 5958 820 Size: 197 926 1765 1087 901 262 1553 Fragments arranged by size: 1765 1553 1087 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 4049 1132 Size: 2917 3774 SspI AAT'ATT Cuts at: 5 52 2326 2399 2560 4615 5 Size: 47 2274 73 161 2055 2081 Fragments arranged by size: 2274 2081 2055 161 73 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6691 StyI C'CwwG_G Cuts at: 513 1065 2739 4130 513 Size: 552 1674 1391 3074 Fragments arranged by size: 3074 1674 1391 552 SwaI ATTT'AAAT Cuts at: 2953 2953 Size: 6691 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4718 4735 4888 5073 5518 325 Size: 1255 3138 17 153 185 445 1498 Fragments arranged by size: 3138 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2587 3313 4937 Size: 274 80 33 51 1443 610 726 1624 Cuts at: 4937 6649 96 Size: 1712 138 Fragments arranged by size: 1712 1624 1443 726 610 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 6651 6683 2403 Size: 32 4216 32 2411 Fragments arranged by size: 4216 2411 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6691 XbaI T'CTAG_A Cuts at: 4027 4027 Size: 6691 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 4016 1870 Size: 2146 4545 XmnI GAAnn'nnTTC Cuts at: 2012 4820 2012 Size: 2808 3883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4499 276 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI XcmI