(Circular) (Six-base) MAPSORT of: pFN21AB8476.seq Check: 5130 from: 1 to: 7243 pFN21AB8476 7243 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2106 5053 278 Size: 53 83 186 936 570 2947 2468 Fragments arranged by size: 2947 2468 936 570 186 83 53 AccI GT'mk_AC Cuts at: 1937 4586 1937 Size: 2649 4594 Acc65I G'GTAC_C Cuts at: 4564 4564 Size: 7243 AclI AA'CG_TT Cuts at: 1563 5370 5743 1563 Size: 3807 373 3063 Fragments arranged by size: 3807 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 2937 2991 3231 3612 3935 5306 6690 Size: 998 307 54 240 381 323 1371 1384 Cuts at: 6690 6958 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1371 998 381 323 307 268 240 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7046 AflIII A'CryG_T Cuts at: 1203 6142 7119 1203 Size: 4939 977 1327 Fragments arranged by size: 4939 1327 977 AgeI A'CCGG_T Cuts at: 6074 6074 Size: 7243 AhdI GACnn_n'nnGTC Cuts at: 5972 5972 Size: 7243 AleI CACnn'nnGTG Cuts at: 1166 3661 1166 Size: 2495 4748 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7243 AlwNI CAG_nnn'CTG Cuts at: 4986 6558 4986 Size: 1572 5671 ApaLI G'TGCA_C Cuts at: 5300 6456 5300 Size: 1156 6087 ApoI r'AATT_y Cuts at: 1493 3016 3097 3285 3830 3974 4238 4552 4778 Size: 1523 81 188 545 144 264 314 226 Cuts at: 4778 4930 7039 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1523 545 314 264 226 188 152 144 81 AseI AT'TA_AT Cuts at: 160 1032 3531 5797 160 Size: 872 2499 2266 1606 Fragments arranged by size: 2499 2266 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 AvaI C'yCGr_G Cuts at: 1870 1943 4568 1870 Size: 73 2625 4545 Fragments arranged by size: 4545 2625 73 BamHI G'GATC_C Cuts at: 4573 4940 4573 Size: 367 6876 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2534 4564 6019 618 Size: 325 217 627 303 444 2030 1455 1842 Fragments arranged by size: 2030 1842 1455 627 444 325 303 217 BanII G_rGCy'C Cuts at: 1391 2683 2860 2943 3280 4562 1391 Size: 1292 177 83 337 1282 4072 Fragments arranged by size: 4072 1292 1282 337 177 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3219 3301 928 Size: 971 1320 82 4870 Fragments arranged by size: 4870 1320 971 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5434 5468 1903 Size: 34 3497 34 3678 Fragments arranged by size: 3678 3497 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3543 5135 6345 3543 Size: 1592 1210 4441 Fragments arranged by size: 4441 1592 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7243 BfrBI ATG'CAT Cuts at: 2416 2416 Size: 7243 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5854 136 Size: 107 122 71 631 605 337 3845 1525 Fragments arranged by size: 3845 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2969 7238 2969 Size: 4269 2974 BlpI GC'TnA_GC Cuts at: 4660 4660 Size: 7243 Bme1580I G_kGCm'C Cuts at: 1790 5304 6460 1790 Size: 3514 1156 2573 Fragments arranged by size: 3514 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7243 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4974 5932 442 Size: 416 53 83 399 24 3557 958 1753 Fragments arranged by size: 3557 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7243 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 5903 1239 Size: 951 3713 2579 Fragments arranged by size: 3713 2579 951 Bpu10I CC'TnA_GC Cuts at: 3186 3186 Size: 7243 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5368 6233 6531 5368 Size: 865 298 6080 Fragments arranged by size: 6080 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2852 5906 882 Size: 617 1353 3054 2219 Fragments arranged by size: 3054 2219 1353 617 BsaAI yAC'GTr Cuts at: 493 1204 5034 493 Size: 711 3830 2702 Fragments arranged by size: 3830 2702 711 BsaBI GATnn'nnATC Cuts at: 3085 7237 3085 Size: 4152 3091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 5050 5432 6067 Size: 53 83 186 936 570 2947 382 635 Cuts at: 6067 275 Size: 1451 Fragments arranged by size: 2947 1451 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3223 5676 6074 6348 6495 6906 3223 Size: 2453 398 274 147 411 3560 Fragments arranged by size: 3560 2453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7018 Fragments arranged by size: 7018 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3033 1186 Size: 1847 5396 BseYI C'CCAG_C Cuts at: 6446 6942 6446 Size: 496 6747 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4185 4482 1163 Size: 573 2449 297 3924 Fragments arranged by size: 3924 2449 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4693 5454 6482 1540 Size: 275 186 2692 761 1028 2301 Fragments arranged by size: 2692 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3280 5304 5389 6460 3280 Size: 2024 85 1071 4063 Fragments arranged by size: 4063 2024 1071 85 BsmI GAATG_Cn' Cuts at: 2989 4754 4847 2989 Size: 1765 93 5385 Fragments arranged by size: 5385 1765 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2683 2860 2943 3280 4562 5304 5389 Size: 399 893 177 83 337 1282 742 85 Cuts at: 5389 6460 1391 Size: 1071 2174 Fragments arranged by size: 2174 1282 1071 893 742 399 337 177 85 83 BspEI T'CCGG_A Cuts at: 3223 6906 3223 Size: 3683 3560 BspHI T'CATG_A Cuts at: 5132 6862 5132 Size: 1730 5513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4598 844 Size: 3754 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5130 1394 Size: 429 3307 3507 Fragments arranged by size: 3507 3307 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2508 5738 5912 65 Size: 1183 1260 3230 174 1396 Fragments arranged by size: 3230 1396 1260 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5887 6074 1700 Size: 110 4077 187 2869 Fragments arranged by size: 4077 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2122 96 Size: 2026 5217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7243 BssSI C'ACGA_G Cuts at: 3066 5303 6315 3066 Size: 2237 1012 3994 Fragments arranged by size: 3994 2237 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7243 BstBI TT'CG_AA Cuts at: 6047 6047 Size: 7243 BstEII G'GTnAC_C Cuts at: 1184 4402 1184 Size: 3218 4025 BstXI CCAn_nnnn'nTGG Cuts at: 2330 2330 Size: 7243 BstYI r'GATC_y Cuts at: 1972 2969 4573 4940 5325 5342 6794 6880 6892 Size: 997 1604 367 385 17 1452 86 12 Cuts at: 6892 7238 1972 Size: 346 1977 Fragments arranged by size: 1977 1604 1452 997 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2039 2039 Size: 7243 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2455 3656 513 Size: 552 939 24 427 1201 4100 Fragments arranged by size: 4100 1201 939 552 427 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2475 487 Size: 683 56 1249 5255 Fragments arranged by size: 5255 1249 683 56 BtsI GCAGTG_nn' Cuts at: 4759 5553 5573 4759 Size: 794 20 6429 Fragments arranged by size: 6429 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7243 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3860 3895 529 Size: 35 3296 35 3877 Fragments arranged by size: 3877 3296 35 35 DraI TTT'AAA Cuts at: 4547 4547 Size: 7243 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4460 6250 809 Size: 507 3144 1790 1802 Fragments arranged by size: 3144 1802 1790 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4690 5579 7208 Size: 54 1425 50 275 18 2860 889 1629 Cuts at: 7208 8 Size: 43 Fragments arranged by size: 2860 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4690 1537 Size: 275 2878 4090 Fragments arranged by size: 4090 2878 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2690 5173 1353 Size: 1337 2483 3423 Fragments arranged by size: 3423 2483 1337 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2059 2316 5830 6204 6350 387 Size: 1064 138 470 257 3514 374 146 1280 Fragments arranged by size: 3514 1280 1064 470 374 257 146 138 EcoICRI GAG'CTC Cuts at: 3278 3278 Size: 7243 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2630 2937 2991 3231 3612 3935 Size: 393 558 440 307 54 240 381 323 Cuts at: 3935 5306 5903 6690 6958 1239 Size: 1371 597 787 268 1524 Fragments arranged by size: 1524 1371 787 597 558 440 393 381 323 307 268 240 54 EcoO109I rG'GnC_Cy Cuts at: 2011 2011 Size: 7243 EcoRI G'AATT_C Cuts at: 4552 4930 4552 Size: 378 6865 EcoRV GAT'ATC Cuts at: 2425 2605 2425 Size: 180 7063 FspI TGC'GCA Cuts at: 5749 5749 Size: 7243 HaeII r_GCGC'y Cuts at: 1678 6390 1678 Size: 4712 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5884 5916 5958 5990 1373 Size: 32 135 32 4312 32 42 32 2626 Fragments arranged by size: 4312 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4587 4833 669 Size: 988 281 2649 246 3079 Fragments arranged by size: 3079 2649 988 281 246 HindIII A'AGCT_T Cuts at: 748 4384 748 Size: 3636 3607 HpaI GTT'AAC Cuts at: 4833 4833 Size: 7243 KpnI G_GTAC'C Cuts at: 4568 4568 Size: 7243 MfeI C'AATT_G Cuts at: 2628 4842 2628 Size: 2214 5029 MluI A'CGCG_T Cuts at: 7119 7119 Size: 7243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4185 6357 6541 1305 Size: 2880 2172 184 2007 Fragments arranged by size: 2880 2172 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5421 Fragments arranged by size: 5421 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 3661 4144 5201 5560 5719 518 Size: 648 1294 1201 483 1057 359 159 2042 Fragments arranged by size: 2042 1294 1201 1057 648 483 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2030 2088 4659 5336 6484 6729 1606 Size: 411 13 58 2571 677 1148 245 2120 Fragments arranged by size: 2571 2120 1148 677 411 245 58 13 NcoI C'CATG_G Cuts at: 513 1065 2004 2455 513 Size: 552 939 451 5301 Fragments arranged by size: 5301 939 552 451 NdeI CA'TA_TG Cuts at: 387 387 Size: 7243 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7243 NotI GC'GGCC_GC Cuts at: 4690 4690 Size: 7243 NsiI A_TGCA'T Cuts at: 2418 2418 Size: 7243 NspI r_CATG'y Cuts at: 1132 1229 3495 3764 4601 6146 1132 Size: 97 2266 269 837 1545 2229 Fragments arranged by size: 2266 2229 1545 837 269 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7243 PciI A'CATG_T Cuts at: 6142 6142 Size: 7243 PflMI CCAn_nnn'nTGG Cuts at: 1108 3314 1108 Size: 2206 5037 PfoI T'CCnGG_A Cuts at: 3545 3545 Size: 7243 PmeI GTTT'AAAC Cuts at: 4547 4547 Size: 7243 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5312 5344 6825 6857 5312 Size: 32 1481 32 5698 Fragments arranged by size: 5698 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7243 PsiI TTA'TAA Cuts at: 4813 4813 Size: 7243 PstI C_TGCA'G Cuts at: 830 2146 4595 830 Size: 1316 2449 3478 Fragments arranged by size: 3478 2449 1316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7243 SacI G_AGCT'C Cuts at: 3280 3280 Size: 7243 SacII CC_GC'GG Cuts at: 2031 2031 Size: 7243 SalI G'TCGA_C Cuts at: 1936 4585 1936 Size: 2649 4594 SbfI CC_TGCA'GG Cuts at: 4595 4595 Size: 7243 ScaI AGT'ACT Cuts at: 5491 7203 5491 Size: 1712 5531 SfcI C'TryA_G Cuts at: 826 1045 2142 4326 4591 5726 6407 6598 826 Size: 219 1097 2184 265 1135 681 191 1471 Fragments arranged by size: 2184 1471 1135 1097 681 265 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7243 SmaI CCC'GGG Cuts at: 1872 4570 1872 Size: 2698 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5347 6248 6510 820 Size: 197 926 3404 901 262 1553 Fragments arranged by size: 3404 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5034 493 Size: 4541 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7243 SphI G_CATG'C Cuts at: 1132 3495 4601 1132 Size: 2363 1106 3774 Fragments arranged by size: 3774 2363 1106 SspI AAT'ATT Cuts at: 5 52 2202 2440 5167 5 Size: 47 2150 238 2727 2081 Fragments arranged by size: 2727 2150 2081 238 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7243 StyI C'CwwG_G Cuts at: 513 1065 2004 2455 3191 4682 513 Size: 552 939 451 736 1491 3074 Fragments arranged by size: 3074 1491 939 736 552 451 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2685 3481 3828 5270 5287 5440 5625 Size: 1255 1105 796 347 1442 17 153 185 Cuts at: 5625 6070 325 Size: 445 1498 Fragments arranged by size: 1498 1442 1255 1105 796 445 347 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2122 3573 3665 Size: 274 80 33 51 1443 145 1451 92 Cuts at: 3665 5489 7201 96 Size: 1824 1712 138 Fragments arranged by size: 1824 1712 1451 1443 274 145 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7203 7235 7203 Size: 32 7211 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7243 XbaI T'CTAG_A Cuts at: 2852 4579 2852 Size: 1727 5516 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7243 XmaI C'CCGG_G Cuts at: 1870 4568 1870 Size: 2698 4545 XmnI GAAnn'nnTTC Cuts at: 2814 5372 2814 Size: 2558 4685 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2104 5051 276 Size: 53 83 186 936 570 2947 2468 Fragments arranged by size: 2947 2468 936 570 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI