Restriction map for
pFN21AE6411
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglII BlnI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 819 GAATTT [GA]^AATT[CT]
ApaI 1006 GGGCCC GGGCC^C
ApoI 819 GAATTT [GA]^AATT[CT]
Asp700I 693 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1720 GGCACC G^G[CT][GA]CC
BbsI 91 GTCTTC GAAGAC(2/6)
BclI 290 TGATCA T^GATCA
BlpI 423 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC
BpuAI 91 GTCTTC GAAGAC(2/6)
BsaBI 670 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1572 GGTCTC GGTCTC(1/5)
BspMI 320 ACCTGC ACCTGC(4/8)
BssHII 38 GCGCGC G^CGCGC
BstBI 503 TTCGAA TT^CGAA
BstEII 396 GGTGACC G^GT[ACGT]ACC
BstPI 396 GGTGACC G^GT[ACGT]ACC
CelII 423 GCTGAGC GC^T[ACGT]AGC
DraIII 565 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 396 GGTGACC G^GT[ACGT]ACC
FbaI 290 TGATCA T^GATCA
MaeII 812 ACGT A^CGT
MamI 670 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1313 CCATGG C^CATGG
NspI 1049 GCATGT [GA]CATG^[CT]
NspV 503 TTCGAA TT^CGAA
PaeR7I 971 CTCGAG C^TCGAG
PflMI 996 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 251 AGTACT AGT^ACT
SfuI 503 TTCGAA TT^CGAA
SmaI 1073 CCCGGG CCC^GGG
StuI 1641 AGGCCT AGG^CCT
Van91I 996 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 971 CTCGAG C^TCGAG
XmaCI 1073 CCCGGG C^CCGGG
XmaI 1073 CCCGGG C^CCGGG
XmnI 693 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Alw44I 156 GTGCAC G^TGCAC
1510 GTGCAC
ApaLI 156 GTGCAC G^TGCAC
1510 GTGCAC
AspHI 156 GTGCAC G[AT]GC[AT]^C
1510 GTGCAC
AvaI 971 CTCGAG C^[CT]CG[GA]G
1073 CCCGGG
BglI 369 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1021 GCCGAACAGGC
BseRI 1063 CTCCTC GAGGAG(10/8)
310 GAGGAG
BsgI 322 CTGCAC GTGCAG(16/14)
1366 CTGCAC
BsiHKAI 156 GTGCAC G[AT]GC[AT]^C
1510 GTGCAC
BsoBI 971 CTCGAG C^[CT]CG[GA]G
1073 CCCGGG
BsrBI 70 CCGCTC CCGCTC(-3/-3)
172 CCGCTC
BsrDI 459 CATTGC GCAATG(2/0)
1766 CATTGC
DsaI 575 CCACGG C^C[GA][CT]GG
1313 CCATGG
FauI 102 CCCGC CCCGC(4/6)
1390 CCCGC
NaeI 369 GCCGGC GCC^GGC
648 GCCGGC
NgoMIV 369 GCCGGC G^CCGGC
648 GCCGGC
PleI 655 GACTC GAGTC(4/5)
362 GAGTC
PpuMI 509 AGGACCC [GA]G^G[AT]CC[CT]
1076 GGGACCC
SgrAI 325 CACCGGCG C[GA]^CCGG[CT]G
1140 CACCGGCG
Tsp509I 820 AATT ^AATT
981 AATT
XcmI 374 CCAGGCTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1706 CCATCTTCCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BalI BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII