(Linear) (Six-base) MAPSORT of: KIEE6411.seq Check: 250 from: 1 to: 1806 KIEE6411.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 900 1806 Size: 900 906 AleI CACnn'nnGTG Cuts at: 0 1144 1806 Size: 1144 662 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1069 1101 1806 Size: 1069 32 705 Fragments arranged by size: 1069 705 32 AlwNI CAG_nnn'CTG Cuts at: 0 544 923 1002 1806 Size: 544 379 79 804 Fragments arranged by size: 804 544 379 79 ApaI G_GGCC'C Cuts at: 0 1010 1806 Size: 1010 796 ApaLI G'TGCA_C Cuts at: 0 156 1510 1806 Size: 156 1354 296 Fragments arranged by size: 1354 296 156 ApoI r'AATT_y Cuts at: 0 819 1806 Size: 819 987 AvaI C'yCGr_G Cuts at: 0 971 1073 1806 Size: 971 102 733 Fragments arranged by size: 971 733 102 BanI G'GyrC_C Cuts at: 0 1720 1806 Size: 1720 86 BanII G_rGCy'C Cuts at: 0 972 1010 1241 1806 Size: 972 38 231 565 Fragments arranged by size: 972 565 231 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 84 1806 Size: 84 1722 BclI T'GATC_A Cuts at: 0 290 1806 Size: 290 1516 BglI GCCn_nnn'nGGC Cuts at: 0 375 1027 1806 Size: 375 652 779 Fragments arranged by size: 779 652 375 BlpI GC'TnA_GC Cuts at: 0 424 1806 Size: 424 1382 Bme1580I G_kGCm'C Cuts at: 0 160 575 739 757 1010 1277 1388 1514 Size: 160 415 164 18 253 267 111 126 Cuts at: 1514 1540 1701 1723 1806 Size: 26 161 22 83 Fragments arranged by size: 415 267 253 164 161 160 126 111 83 26 22 18 BmgBI CAC'GTC Cuts at: 0 813 1806 Size: 813 993 BmrI ACTGGGnnnn_n' Cuts at: 0 243 1743 1806 Size: 243 1500 63 Fragments arranged by size: 1500 243 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 642 945 1396 1806 Size: 642 303 451 410 Fragments arranged by size: 642 451 410 303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 772 1180 1806 Size: 325 447 408 626 Fragments arranged by size: 626 447 408 325 BsaI GGTCTCn'nnnn_ Cuts at: 0 1578 1806 Size: 1578 228 BsaBI GATnn'nnATC Cuts at: 0 674 1806 Size: 674 1132 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 421 451 571 601 1806 Size: 421 30 120 30 1205 Fragments arranged by size: 1205 421 120 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 325 1054 1806 Size: 325 729 752 Fragments arranged by size: 752 729 325 BseYI C'CCAG_C Cuts at: 0 514 1097 1716 1806 Size: 514 583 619 90 Fragments arranged by size: 619 583 514 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1351 1806 Size: 307 1044 455 Fragments arranged by size: 1044 455 307 BsiHKAI G_wGCw'C Cuts at: 0 160 1514 1806 Size: 160 1354 292 Fragments arranged by size: 1354 292 160 Bsp1286I G_dGCh'C Cuts at: 0 160 575 739 757 972 1010 1241 1277 Size: 160 415 164 18 215 38 231 36 Cuts at: 1277 1388 1514 1540 1701 1723 1806 Size: 111 126 26 161 22 83 Fragments arranged by size: 415 231 215 164 161 160 126 111 83 38 36 26 22 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 329 1806 Size: 329 1477 BsrBI CCG'CTC Cuts at: 0 72 174 1806 Size: 72 102 1632 Fragments arranged by size: 1632 102 72 BsrDI GCAATG_nn' Cuts at: 0 458 1765 1806 Size: 458 1307 41 Fragments arranged by size: 1307 458 41 BsrFI r'CCGG_y Cuts at: 0 84 326 369 648 1141 1476 1806 Size: 84 242 43 279 493 335 330 Fragments arranged by size: 493 335 330 279 242 84 43 BssHII G'CGCG_C Cuts at: 0 38 1806 Size: 38 1768 BstBI TT'CG_AA Cuts at: 0 504 1806 Size: 504 1302 BstEII G'GTnAC_C Cuts at: 0 396 1806 Size: 396 1410 BstYI r'GATC_y Cuts at: 0 344 642 887 1806 Size: 344 298 245 919 Fragments arranged by size: 919 344 298 245 BtgI C'CryG_G Cuts at: 0 575 1313 1806 Size: 575 738 493 Fragments arranged by size: 738 575 493 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1407 1578 1806 Size: 1407 171 228 Fragments arranged by size: 1407 228 171 BtsI GCAGTG_nn' Cuts at: 0 440 811 1806 Size: 440 371 995 Fragments arranged by size: 995 440 371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1760 1795 1806 Size: 1760 35 11 Fragments arranged by size: 1760 35 11 DraIII CAC_nnn'GTG Cuts at: 0 570 1806 Size: 570 1236 EaeI y'GGCC_r Cuts at: 0 20 296 371 489 780 1316 1493 1781 Size: 20 276 75 118 291 536 177 288 Cuts at: 1781 1806 Size: 25 Fragments arranged by size: 536 291 288 276 177 118 75 25 20 EarI CTCTTCn'nnn_ Cuts at: 0 280 524 1072 1114 1806 Size: 280 244 548 42 692 Fragments arranged by size: 692 548 280 244 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1489 1806 Size: 1489 317 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 642 900 945 1396 1806 Size: 642 258 45 451 410 Fragments arranged by size: 642 451 410 258 45 EcoO109I rG'GnC_Cy Cuts at: 0 165 510 1006 1077 1187 1806 Size: 165 345 496 71 110 619 Fragments arranged by size: 619 496 345 165 110 71 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 813 1806 Size: 813 993 HaeII r_GCGC'y Cuts at: 0 10 190 305 1806 Size: 10 180 115 1501 Fragments arranged by size: 1501 180 115 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1732 1764 1806 Size: 1732 32 42 Fragments arranged by size: 1732 42 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 257 339 661 1806 Size: 257 82 322 1145 Fragments arranged by size: 1145 322 257 82 MscI TGG'CCA Cuts at: 0 298 491 782 1318 1495 1783 1806 Size: 298 193 291 536 177 288 23 Fragments arranged by size: 536 298 291 288 193 177 23 MslI CAynn'nnrTG Cuts at: 0 145 445 580 1144 1414 1806 Size: 145 300 135 564 270 392 Fragments arranged by size: 564 392 300 270 145 135 MspA1I CmG'CkG Cuts at: 0 19 119 541 1365 1716 1806 Size: 19 100 422 824 351 90 Fragments arranged by size: 824 422 351 100 90 19 NaeI GCC'GGC Cuts at: 0 371 650 1806 Size: 371 279 1156 Fragments arranged by size: 1156 371 279 NcoI C'CATG_G Cuts at: 0 1313 1806 Size: 1313 493 NgoMIV G'CCGG_C Cuts at: 0 369 648 1806 Size: 369 279 1158 Fragments arranged by size: 1158 369 279 NspI r_CATG'y Cuts at: 0 1053 1806 Size: 1053 753 PasI CC'CwG_GG Cuts at: 0 619 1806 Size: 619 1187 PflMI CCAn_nnn'nTGG Cuts at: 0 1002 1806 Size: 1002 804 PfoI T'CCnGG_A Cuts at: 0 658 1727 1806 Size: 658 1069 79 Fragments arranged by size: 1069 658 79 PpuMI rG'GwC_Cy Cuts at: 0 510 1077 1806 Size: 510 567 729 Fragments arranged by size: 729 567 510 PspOMI G'GGCC_C Cuts at: 0 1006 1806 Size: 1006 800 PspXI vC'TCGA_Gb Cuts at: 0 971 1806 Size: 971 835 PvuII CAG'CTG Cuts at: 0 541 1365 1716 1806 Size: 541 824 351 90 Fragments arranged by size: 824 541 351 90 SanDI GG'GwC_CC Cuts at: 0 1077 1806 Size: 1077 729 ScaI AGT'ACT Cuts at: 0 253 1806 Size: 253 1553 SgrAI Cr'CCGG_yG Cuts at: 0 326 1141 1806 Size: 326 815 665 Fragments arranged by size: 815 665 326 SmaI CCC'GGG Cuts at: 0 1075 1806 Size: 1075 731 SmlI C'TyrA_G Cuts at: 0 340 787 971 1195 1806 Size: 340 447 184 224 611 Fragments arranged by size: 611 447 340 224 184 StuI AGG'CCT Cuts at: 0 1643 1806 Size: 1643 163 StyI C'CwwG_G Cuts at: 0 107 1313 1778 1806 Size: 107 1206 465 28 Fragments arranged by size: 1206 465 107 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1651 1740 1788 1806 Size: 1651 89 48 18 Fragments arranged by size: 1651 89 48 18 TatI w'GTAC_w Cuts at: 0 149 251 1745 1806 Size: 149 102 1494 61 Fragments arranged by size: 1494 149 102 61 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 381 1713 1806 Size: 381 1332 93 Fragments arranged by size: 1332 381 93 XhoI C'TCGA_G Cuts at: 0 971 1806 Size: 971 835 XmaI C'CCGG_G Cuts at: 0 1073 1806 Size: 1073 733 XmnI GAAnn'nnTTC Cuts at: 0 697 1806 Size: 697 1109 Enzymes that do cut: AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PspXI PvuII SanDI ScaI SgrAI SmaI SmlI StuI StyI TaqII TatI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstAPI BstXI BstZ17I Bsu36I ClaI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI ZraI