(Circular) (Six-base) MAPSORT of: pFN21AE6234.seq Check: 6629 from: 1 to: 5590 pFN21AE6234 5590 bp With 182 enzymes: SgfI * July 20, 2018 18:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2390 2390 Size: 5590 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2208 2911 2208 Size: 703 4887 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 5037 5305 1632 Size: 2021 1384 268 1917 Fragments arranged by size: 2021 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 2457 Size: 32 5558 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2343 2920 3287 2343 Size: 577 367 4646 Fragments arranged by size: 4646 577 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2208 2275 2614 2911 4366 Size: 325 217 627 421 67 339 297 1455 Cuts at: 4366 618 Size: 1842 Fragments arranged by size: 1842 1455 627 421 339 325 297 217 67 BanII G_rGCy'C Cuts at: 1391 2134 2462 2909 1391 Size: 743 328 447 4072 Fragments arranged by size: 4072 743 447 328 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 2518 2710 2879 928 Size: 971 171 448 192 169 3639 Fragments arranged by size: 3639 971 448 192 171 169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1998 2032 3781 3815 1903 Size: 34 61 34 1749 34 3678 Fragments arranged by size: 3678 1749 61 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 2676 1548 Size: 1128 4462 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4201 136 Size: 107 122 71 631 605 2529 1525 Fragments arranged by size: 2529 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 2619 3651 4807 1790 Size: 829 1032 1156 2573 Fragments arranged by size: 2573 1156 1032 829 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2461 3321 4279 Size: 416 53 83 399 24 1044 860 958 Cuts at: 4279 442 Size: 1753 Fragments arranged by size: 1753 1044 958 860 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2043 2043 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2484 2873 4250 1239 Size: 1245 389 1377 2579 Fragments arranged by size: 2579 1377 1245 389 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3715 4580 4878 3715 Size: 865 298 4427 Fragments arranged by size: 4427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 2262 4253 882 Size: 617 515 248 1991 2219 Fragments arranged by size: 2219 1991 617 515 248 BsaAI yAC'GTr Cuts at: 493 1204 2735 3381 493 Size: 711 1531 646 2702 Fragments arranged by size: 2702 1531 711 646 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2346 2586 4023 4421 4695 4842 5253 2346 Size: 240 1437 398 274 147 411 2683 Fragments arranged by size: 2683 1437 411 398 274 240 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 2457 2487 1178 Size: 30 165 30 786 30 238 30 4281 Fragments arranged by size: 4281 786 238 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2048 2051 2054 2057 2426 2468 2489 Size: 859 3 3 3 3 369 42 21 Cuts at: 2489 2861 2868 1186 Size: 372 7 3908 Fragments arranged by size: 3908 859 372 369 42 21 7 3 3 3 3 BseYI C'CCAG_C Cuts at: 2033 4793 5289 2033 Size: 2760 496 2334 Fragments arranged by size: 2760 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5017 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 3651 3736 4807 2499 Size: 1152 85 1071 3282 Fragments arranged by size: 3282 1152 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2134 2462 2499 2619 2909 3651 3736 Size: 399 344 328 37 120 290 742 85 Cuts at: 3736 4807 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 328 290 120 85 37 BspEI T'CCGG_A Cuts at: 2346 5253 2346 Size: 2907 2683 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2390 2945 844 Size: 1546 555 3489 Fragments arranged by size: 3489 1546 555 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 4085 4259 65 Size: 1183 1179 1658 174 1396 Fragments arranged by size: 1658 1396 1183 1179 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2650 4234 4421 1700 Size: 110 840 1584 187 2869 Fragments arranged by size: 2869 1584 840 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2343 2440 2920 3287 3672 3689 5141 5227 Size: 371 97 480 367 385 17 1452 86 Cuts at: 5227 5239 5585 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 480 385 371 367 346 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 3106 3900 3920 3106 Size: 794 20 4776 Fragments arranged by size: 4776 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555 Size: 54 1425 50 275 18 1207 889 1629 Cuts at: 5555 8 Size: 43 Fragments arranged by size: 1629 1425 1207 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2721 2749 2800 3520 1353 Size: 1368 28 51 720 3423 Fragments arranged by size: 3423 1368 720 51 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2484 2873 3653 4250 5037 5305 1239 Size: 393 852 389 780 597 787 268 1524 Fragments arranged by size: 1524 852 787 780 597 393 389 268 EcoNI CCTnn'n_nnAGG Cuts at: 2224 2224 Size: 5590 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 4737 1678 Size: 3059 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2109 2141 2563 2581 2595 Size: 32 135 32 537 32 422 18 14 Cuts at: 2595 2613 4231 4263 4305 4337 1373 Size: 18 1618 32 42 32 2626 Fragments arranged by size: 2626 1618 537 422 135 42 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 2011 748 Size: 1263 4327 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2212 2915 2212 Size: 703 4887 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3768 Fragments arranged by size: 3768 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 3548 3907 4066 518 Size: 648 1032 1350 359 159 2042 Fragments arranged by size: 2042 1350 1032 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 3006 3683 4831 5076 1606 Size: 431 969 677 1148 245 2120 Fragments arranged by size: 2120 1148 969 677 431 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2948 4493 1132 Size: 97 1719 1545 2229 Fragments arranged by size: 2229 1719 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2457 2489 3659 3691 5172 5204 2457 Size: 32 1170 32 1481 32 2843 Fragments arranged by size: 2843 1481 1170 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 2757 3160 2757 Size: 403 5187 PstI C_TGCA'G Cuts at: 830 2508 2541 2942 830 Size: 1678 33 401 3478 Fragments arranged by size: 3478 1678 401 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2037 2037 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SapI GCTCTTCn'nnn_ Cuts at: 2721 2721 Size: 5590 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2187 2242 2504 2537 2938 4073 4754 Size: 219 1142 55 262 33 401 1135 681 Cuts at: 4754 4945 826 Size: 191 1471 Fragments arranged by size: 1471 1142 1135 681 401 262 219 191 55 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3694 4595 4857 820 Size: 197 926 1751 901 262 1553 Fragments arranged by size: 1751 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2735 3381 493 Size: 2242 646 2702 Fragments arranged by size: 2702 2242 646 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 2664 3029 513 Size: 552 939 660 365 3074 Fragments arranged by size: 3074 939 660 552 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2443 2852 3617 3634 3787 3972 4417 Size: 1255 863 409 765 17 153 185 445 Cuts at: 4417 325 Size: 1498 Fragments arranged by size: 1498 1255 863 765 445 409 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 3836 5548 Size: 274 80 33 51 1443 564 1295 1712 Cuts at: 5548 96 Size: 138 Fragments arranged by size: 1712 1443 1295 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 5550 5582 2189 Size: 32 3329 32 2197 Fragments arranged by size: 3329 2197 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI