################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 252 GTAC GT^AC AflII 10 CTTAAG C^TTAAG Asp700I 45 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 277 GAGCTC G[AT]GC[AT]^C AvaI 282 CCCGAG C^[CT]CG[GA]G BbvI 159 GCAGC GCAGC(8/12) BcgI 201 GCAGGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 10 CTTAAG C^TTAAG BseRI 300 CTCCTC GAGGAG(10/8) BsiHKAI 277 GAGCTC G[AT]GC[AT]^C BsmBI 93 CGTCTC CGTCTC(1/5) BsmFI 257 GGGAC GGGAC(10/14) BsoBI 282 CCCGAG C^[CT]CG[GA]G BspHI 273 TCATGA T^CATGA BsrI 128 ACTGG ACTGG(1/-1) BssKI 243 CCTGG ^CC[ACGT]GG BstF5I 131 GGATG GGATG(2/0) BstNI 243 CCTGG CC^[AT]GG BstYI 24 GGATCT [GA]^GATC[CT] EarI 5 CTCTTC CTCTTC(1/4) EcoRII 243 CCTGG ^CC[AT]GG Fnu4HI 159 GCAGC GC^[ACGT]GC FokI 131 GGATG GGATG(9/13) ItaI 159 GCAGC GC^[ACGT]GC Ksp632I 5 CTCTTC CTCTTC(1/4) MflI 24 GGATCT [GA]^GATC[CT] MseI 11 TTAA T^TAA MvaI 243 CCTGG CC^[AT]GG NlaIV 174 GGAGCC GG[ACGT]^[ACGT]CC PleI 285 GAGTC GAGTC(4/5) PstI 199 CTGCAG CTGCA^G RcaI 273 TCATGA T^CATGA RsaI 252 GTAC GT^AC SacI 277 GAGCTC GAGCT^C SapI 4 GCTCTTC GCTCTTC(1/4) ScrFI 243 CCTGG CC^[ACGT]GG SfcI 199 CTGCAG C^T[GA][CT]AG SmlI 10 CTTAAG C^T[CT][GA]AG TfiI 66 GAATC G^A[AT]TC Tru9I 11 TTAA T^TAA TseI 159 GCAGC G^C[AT]GC XcmI 233 CCAACCCTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 24 GGATCT [GA]^GATC[CT] XmnI 45 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 35 CCGC CCGC(-3/-1) 295 CCGC AlwI 85 GATCC GGATC(4/5) 24 GGATC BalI 230 TGGCCA TGG^CCA 245 TGGCCA BanII 175 GAGCCC G[GA]GC[CT]^C 277 GAGCTC BbsI 103 GAAGAC GAAGAC(2/6) 76 GTCTTC BmyI 175 GAGCCC G[AGT]GC[ACT]^C 277 GAGCTC BpuAI 103 GAAGAC GAAGAC(2/6) 76 GTCTTC BsiYI 237 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 248 CCAAGTACAGG BslI 237 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 248 CCAAGTACAGG Bsp1286I 175 GAGCCC G[AGT]GC[ACT]^C 277 GAGCTC BspMI 72 GCAGGT ACCTGC(4/8) 162 GCAGGT DpnII 25 GATC ^GATC 85 GATC EaeI 230 TGGCCA [CT]^GGCC[GA] 245 TGGCCA HinfI 66 GAATC G^A[ACGT]TC 285 GAGTC MboI 25 GATC ^GATC 85 GATC MluNI 230 TGGCCA TGG^CCA 245 TGGCCA MscI 230 TGGCCA TGG^CCA 245 TGGCCA MwoI 63 GCTGAATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 177 GCCCCAAGAGC NdeII 25 GATC ^GATC 85 GATC NlaIII 108 CATG CATG^ 274 CATG Sau3AI 25 GATC ^GATC 85 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI BsmAI HaeIII HphI MaeIII MboII MnlI Tsp45I