################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BseRI BsmI BspDI BspEI BsrDI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII SapI SbfI ScaI SfuI SnaBI SpeI Sse8387I SspI SwaI Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 988 GGTACC G^GTACC AccI 430 GTCGAC GT^[AC][GT]AC AlwNI 334 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 626 AGCGCT AGC^GCT Asp718I 988 GGTACC G^GTACC AviII 1364 TGCGCA TGC^GCA BanII 742 GAGCTC G[GA]GC[CT]^C BbsI 448 GTCTTC GAAGAC(2/6) BciVI 901 GTATCC GTATCC(6/5) BpuAI 448 GTCTTC GAAGAC(2/6) BsaI 1215 GGTCTC GGTCTC(1/5) BsiEI 141 CGATCG CG[GA][CT]^CG BsiWI 473 CGTACG C^GTACG BsmBI 1087 GAGACG CGTCTC(1/5) BspHI 101 TCATGA T^CATGA BstXI 1421 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 1705 CGGACCG CG^G[AT]CCG DraIII 193 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 211 CTCTTC CTCTTC(1/4) Eco47III 626 AGCGCT AGC^GCT FspI 1364 TGCGCA TGC^GCA KpnI 988 GGTACC GGTAC^C Ksp632I 211 CTCTTC CTCTTC(1/4) MseI 806 TTAA T^TAA NcoI 728 CCATGG C^CATGG PflMI 333 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 29 CTGCAG CTGCA^G PvuI 141 CGATCG CGAT^CG RcaI 101 TCATGA T^CATGA RsrII 1705 CGGACCG CG^G[AT]CCG SacI 742 GAGCTC GAGCT^C SalI 430 GTCGAC G^TCGAC SexAI 194 ACCTGGT A^CC[AT]GGT SfcI 29 CTGCAG C^T[GA][CT]AG SgrAI 229 CACCGGCG C[GA]^CCGG[CT]G SmaI 770 CCCGGG CCC^GGG SmlI 958 CTTGAG C^T[CT][GA]AG SphI 1031 GCATGC GCATG^C TfiI 679 GATTC G^A[AT]TC Tru9I 806 TTAA T^TAA Van91I 333 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 770 CCCGGG C^CCGGG XmaI 770 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AspHI 226 GAGCAC G[AT]GC[AT]^C 742 GAGCTC AvaI 770 CCCGGG C^[CT]CG[GA]G 1467 CTCGGG BalI 1325 TGGCCA TGG^CCA 1655 TGGCCA BamHI 564 GGATCC G^GATCC 800 GGATCC BbeI 532 GGCGCC GGCGC^C 1633 GGCGCC BfaI 922 CTAG C^TAG 1435 CTAG BlpI 1035 GCTCAGC GC^T[ACGT]AGC 1113 GCTGAGC Bpu1102I 1035 GCTCAGC GC^T[ACGT]AGC 1113 GCTGAGC BsgI 694 GTGCAG GTGCAG(16/14) 1309 GTGCAG BsiHKAI 226 GAGCAC G[AT]GC[AT]^C 742 GAGCTC BsoBI 770 CCCGGG C^[CT]CG[GA]G 1467 CTCGGG Bsp1407I 653 TGTACA T^GTACA 1160 TGTACA BspLU11I 434 ACATGT A^CATGT 1400 ACATGT BspMI 326 ACCTGC ACCTGC(4/8) 696 GCAGGT BsrBI 871 GAGCGG CCGCTC(-3/-3) 1584 GAGCGG BsrGI 653 TGTACA T^GTACA 1160 TGTACA BssSI 1564 CACGAG CACGAG(-5/-1) 745 CTCGTG BstYI 564 GGATCC [GA]^GATC[CT] 800 GGATCC CelII 1035 GCTCAGC GC^T[ACGT]AGC 1113 GCTGAGC HincII 430 GTCGAC GT[CT]^[GA]AC 1662 GTTGAC HindII 430 GTCGAC GT[CT]^[GA]AC 1662 GTTGAC KasI 532 GGCGCC G^GCGCC 1633 GGCGCC KspI 512 CCGCGG CCGC^GG 1622 CCGCGG MaeI 922 CTAG C^TAG 1435 CTAG MaeIII 704 GTGAC ^GT[ACGT]AC 1438 GTCAC MflI 564 GGATCC [GA]^GATC[CT] 800 GGATCC MluNI 1325 TGGCCA TGG^CCA 1655 TGGCCA MscI 1325 TGGCCA TGG^CCA 1655 TGGCCA MslI 390 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 471 CACGTACGTG NarI 532 GGCGCC GG^CGCC 1633 GGCGCC SacII 512 CCGCGG CCGC^GG 1622 CCGCGG SfaNI 265 GATGC GCATC(5/9) 908 GCATC SfoI 532 GGCGCC GGC^GCC 1633 GGCGCC SspBI 653 TGTACA T^GTACA 1160 TGTACA TaqI 431 TCGA T^CGA 682 TCGA Tsp45I 704 GTGAC ^GT[GC]AC 1438 GTCAC TthHB8I 431 TCGA T^CGA 682 TCGA XcmI 728 CCATGGCACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 993 CCACTGTACTTTTGG XhoII 564 GGATCC [GA]^GATC[CT] 800 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AvaII BanI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfiI StuI StyI TseI TspRI