Restriction map for
pFN21AE1524
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BbsI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaBI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 226 GTCTAC GT^[AC][GT]AC
AccIII 1859 TCCGGA T^CCGGA
AcsI 1550 AAATTC [GA]^AATT[CT]
ApoI 1550 AAATTC [GA]^AATT[CT]
AvrII 1027 CCTAGG C^CTAGG
BalI 1973 TGGCCA TGG^CCA
BbrPI 535 CACGTG CAC^GTG
BbvCI 1624 CCTCAGC CCTCAGC(-5/-2)
BglI 467 GCCCGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1027 CCTAGG C^CTAGG
BsaAI 535 CACGTG [CT]AC^GT[GA]
BsaI 731 GAGACC GGTCTC(1/5)
BsaWI 1859 TCCGGA [AT]^CCGG[AT]
BseAI 1859 TCCGGA T^CCGGA
BsiEI 1095 CGACCG CG[GA][CT]^CG
BsmI 1697 GAATGC GAATGC(1/-1)
BspEI 1859 TCCGGA T^CCGGA
BspHI 512 TCATGA T^CATGA
BssHII 616 GCGCGC G^CGCGC
BssSI 652 CTCGTG CACGAG(-5/-1)
BstYI 233 AGATCC [GA]^GATC[CT]
EcoNI 495 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 154 GGCCGGCC GGCCGG^CC
KspI 714 CCGCGG CCGC^GG
MflI 233 AGATCC [GA]^GATC[CT]
MluNI 1973 TGGCCA TGG^CCA
MroI 1859 TCCGGA T^CCGGA
MscI 1973 TGGCCA TGG^CCA
MseI 89 TTAA T^TAA
NaeI 155 GCCGGC GCC^GGC
NgoMIV 155 GCCGGC G^CCGGC
PflMI 1241 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 535 CACGTG CAC^GTG
PmlI 535 CACGTG CAC^GTG
RcaI 512 TCATGA T^CATGA
SacII 714 CCGCGG CCGC^GG
SapI 1881 GCTCTTC GCTCTTC(1/4)
Tru9I 89 TTAA T^TAA
Tsp509I 1551 AATT ^AATT
Van91I 1241 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 695 CCATGGCGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 233 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 534 ACACGT A^C[GA][CT]GT
914 ACGTGT
Alw44I 388 GTGCAC G^TGCAC
724 GTGCAC
ApaLI 388 GTGCAC G^TGCAC
724 GTGCAC
AvaI 818 CCCGGG C^[CT]CG[GA]G
1364 CCCGGG
BbeI 594 GGCGCC GGCGC^C
1183 GGCGCC
BsgI 547 CTGCAC GTGCAG(16/14)
610 CTGCAC
BsoBI 818 CCCGGG C^[CT]CG[GA]G
1364 CCCGGG
BstEII 1204 GGTGACC G^GT[ACGT]ACC
1797 GGTCACC
BstPI 1204 GGTGACC G^GT[ACGT]ACC
1797 GGTCACC
BstXI 773 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1484 CCAGGAGTCTGG
EaeI 100 CGGCCA [CT]^GGCC[GA]
1973 TGGCCA
EarI 670 CTCTTC CTCTTC(1/4)
1882 CTCTTC
EcoO65I 1204 GGTGACC G^GT[ACGT]ACC
1797 GGTCACC
HaeII 594 GGCGCC [GA]GCGC^[CT]
1183 GGCGCC
KasI 594 GGCGCC G^GCGCC
1183 GGCGCC
Ksp632I 670 CTCTTC CTCTTC(1/4)
1882 CTCTTC
NarI 594 GGCGCC GG^CGCC
1183 GGCGCC
NcoI 695 CCATGG C^CATGG
1691 CCATGG
NspI 1153 GCATGT [GA]CATG^[CT]
1454 ACATGC
PstI 574 CTGCAG CTGCA^G
997 CTGCAG
PvuII 682 CAGCTG CAG^CTG
1952 CAGCTG
SfcI 574 CTGCAG C^T[GA][CT]AG
997 CTGCAG
SfoI 594 GGCGCC GGC^GCC
1183 GGCGCC
SmaI 818 CCCGGG CCC^GGG
1364 CCCGGG
TaqI 1223 TCGA T^CGA
1554 TCGA
TthHB8I 1223 TCGA T^CGA
1554 TCGA
XmaCI 818 CCCGGG C^CCGGG
1364 CCCGGG
XmaI 818 CCCGGG C^CCGGG
1364 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StuI StyI
TseI Tsp45I TspRI