(Circular) (Six-base) MAPSORT of: pFN21AE1524.seq Check: 8062 from: 1 to: 6688 pFN21AE1524 6688 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4498 278 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2232 4031 1937 Size: 295 1799 4594 Fragments arranged by size: 4594 1799 295 Acc65I G'GTAC_C Cuts at: 4009 4009 Size: 6688 AclI AA'CG_TT Cuts at: 1563 4815 5188 1563 Size: 3252 373 3063 Fragments arranged by size: 3252 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 4751 6135 6403 1632 Size: 425 2694 1384 268 1917 Fragments arranged by size: 2694 1917 1384 425 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6491 AflIII A'CryG_T Cuts at: 1203 2539 2919 5587 6564 1203 Size: 1336 380 2668 977 1327 Fragments arranged by size: 2668 1336 1327 977 380 AgeI A'CCGG_T Cuts at: 5519 5519 Size: 6688 AhdI GACnn_n'nnGTC Cuts at: 5417 5417 Size: 6688 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6688 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2774 1184 Size: 1590 5098 AlwNI CAG_nnn'CTG Cuts at: 2441 2578 3010 4431 6003 2441 Size: 137 432 1421 1572 3126 Fragments arranged by size: 3126 1572 1421 432 137 ApaI G_GGCC'C Cuts at: 2719 2850 3058 3088 2719 Size: 131 208 30 6319 Fragments arranged by size: 6319 208 131 30 ApaLI G'TGCA_C Cuts at: 2393 2729 4745 5901 2393 Size: 336 2016 1156 3180 Fragments arranged by size: 3180 2016 1156 336 ApoI r'AATT_y Cuts at: 1493 3555 3997 4223 4375 6484 1493 Size: 2062 442 226 152 2109 1697 Fragments arranged by size: 2109 2062 1697 442 226 152 AseI AT'TA_AT Cuts at: 160 1032 5242 160 Size: 872 4210 1606 Fragments arranged by size: 4210 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 AvaI C'yCGr_G Cuts at: 1870 1943 2823 3369 4013 1870 Size: 73 880 546 644 4545 Fragments arranged by size: 4545 880 644 546 73 AvrII C'CTAG_G Cuts at: 3032 3032 Size: 6688 BamHI G'GATC_C Cuts at: 4018 4385 4018 Size: 367 6321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2298 2599 2819 3188 3359 Size: 325 217 627 511 301 220 369 171 Cuts at: 3359 4009 5464 618 Size: 650 1455 1842 Fragments arranged by size: 1842 1455 650 627 511 369 325 301 220 217 171 BanII G_rGCy'C Cuts at: 1391 2274 2612 2719 2850 3025 3058 3088 3715 Size: 883 338 107 131 175 33 30 627 Cuts at: 3715 4007 1391 Size: 292 4072 Fragments arranged by size: 4072 883 627 338 292 175 131 107 33 30 BbeI G_GCGC'C Cuts at: 2603 3192 2603 Size: 589 6099 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5717 BbvCI CC'TCA_GC Cuts at: 3630 3630 Size: 6688 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4879 4913 1903 Size: 34 50 34 2858 34 3678 Fragments arranged by size: 3678 2858 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4580 5790 4580 Size: 1210 5478 BclI T'GATC_A Cuts at: 1548 1548 Size: 6688 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2478 5299 136 Size: 107 122 71 631 605 806 2821 1525 Fragments arranged by size: 2821 1525 806 631 605 122 107 71 BglII A'GATC_T Cuts at: 6683 6683 Size: 6688 BlpI GC'TnA_GC Cuts at: 4105 4105 Size: 6688 Bme1580I G_kGCm'C Cuts at: 1790 2397 2483 2719 2733 2824 2850 2878 3058 Size: 607 86 236 14 91 26 28 180 Cuts at: 3058 3088 3579 4749 5905 1790 Size: 30 491 1170 1156 2573 Fragments arranged by size: 2573 1170 1156 607 491 236 180 91 86 30 28 26 14 BmgBI CAC'GTC Cuts at: 1318 2920 1318 Size: 1602 5086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2059 2236 2412 Size: 416 53 83 399 24 642 177 176 Cuts at: 2412 2429 2498 2576 2615 3849 4419 5377 442 Size: 17 69 78 39 1234 570 958 1753 Fragments arranged by size: 1753 1234 958 642 570 416 399 177 176 83 78 69 53 39 24 17 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6688 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2038 2971 2038 Size: 933 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3032 3034 3143 3488 3518 3573 3729 3756 Size: 1793 2 109 345 30 55 156 27 Cuts at: 3756 3953 5348 1239 Size: 197 1395 2579 Fragments arranged by size: 2579 1793 1395 345 197 156 109 55 30 27 2 Bpu10I CC'TnA_GC Cuts at: 2259 2307 3630 2259 Size: 48 1323 5317 Fragments arranged by size: 5317 1323 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 2309 3857 4813 5678 5976 2105 Size: 204 1548 956 865 298 2817 Fragments arranged by size: 2817 1548 956 865 298 204 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2730 5351 882 Size: 617 1231 2621 2219 Fragments arranged by size: 2621 2219 1231 617 BsaAI yAC'GTr Cuts at: 493 1204 2542 4479 493 Size: 711 1338 1937 2702 Fragments arranged by size: 2702 1937 1338 711 BsaBI GATnn'nnATC Cuts at: 6682 6682 Size: 6688 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2600 2765 3189 4495 Size: 53 83 186 936 1067 165 424 1306 Cuts at: 4495 4877 5512 275 Size: 382 635 1451 Fragments arranged by size: 1451 1306 1067 936 635 424 382 186 165 83 53 BsaWI w'CCGG_w Cuts at: 3864 5121 5519 5793 5940 6351 3864 Size: 1257 398 274 147 411 4201 Fragments arranged by size: 4201 1257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2286 2316 3837 3867 1178 Size: 30 165 30 883 30 1521 30 3999 Fragments arranged by size: 3999 1521 883 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 3466 3618 1186 Size: 916 1364 152 4256 Fragments arranged by size: 4256 1364 916 152 BseYI C'CCAG_C Cuts at: 2169 2368 3081 3295 3436 3635 5891 6387 2169 Size: 199 713 214 141 199 2256 496 2470 Fragments arranged by size: 2470 2256 713 496 214 199 199 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 2600 1163 Size: 573 801 63 5251 Fragments arranged by size: 5251 801 573 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 4138 4899 5927 1540 Size: 275 186 1102 1035 761 1028 2301 Fragments arranged by size: 2301 1102 1035 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2265 2397 2535 2733 4749 4834 5905 2115 Size: 150 132 138 198 2016 85 1071 2898 Fragments arranged by size: 2898 2016 1071 198 150 138 132 85 BsmI GAATG_Cn' Cuts at: 3708 4199 4292 3708 Size: 491 93 6104 Fragments arranged by size: 6104 491 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2265 2274 2397 2483 2535 2612 Size: 399 325 150 9 123 86 52 77 Cuts at: 2612 2719 2733 2824 2850 2878 3025 3058 3088 Size: 107 14 91 26 28 147 33 30 Cuts at: 3088 3579 3715 4007 4749 4834 5905 1391 Size: 491 136 292 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 491 399 325 292 150 147 136 123 107 91 86 85 77 52 33 30 28 26 14 9 BspEI T'CCGG_A Cuts at: 3864 6351 3864 Size: 2487 4201 BspHI T'CATG_A Cuts at: 2517 4577 6307 2517 Size: 2060 1730 2898 Fragments arranged by size: 2898 2060 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4043 844 Size: 3199 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4575 1394 Size: 429 2752 3507 Fragments arranged by size: 3507 2752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5183 5357 65 Size: 1183 3935 174 1396 Fragments arranged by size: 3935 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2160 3102 3903 5332 5519 1700 Size: 110 350 942 801 1429 187 2869 Fragments arranged by size: 2869 1429 942 801 350 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6688 BssHII G'CGCG_C Cuts at: 1925 2621 1925 Size: 696 5992 BssSI C'ACGA_G Cuts at: 2657 4748 5760 2657 Size: 2091 1012 3585 Fragments arranged by size: 3585 2091 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6688 BstBI TT'CG_AA Cuts at: 5492 5492 Size: 6688 BstEII G'GTnAC_C Cuts at: 1184 3209 3802 1184 Size: 2025 593 4070 Fragments arranged by size: 4070 2025 593 BstXI CCAn_nnnn'nTGG Cuts at: 2785 3496 2785 Size: 711 5977 BstYI r'GATC_y Cuts at: 1972 2238 4018 4385 4770 4787 6239 6325 6337 Size: 266 1780 367 385 17 1452 86 12 Cuts at: 6337 6683 1972 Size: 346 1977 Fragments arranged by size: 1977 1780 1452 385 367 346 266 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2013 2133 3194 2013 Size: 120 1061 5507 Fragments arranged by size: 5507 1061 120 BtgI C'CryG_G Cuts at: 513 1065 2294 2700 2719 2893 3696 513 Size: 552 1229 406 19 174 803 3505 Fragments arranged by size: 3505 1229 803 552 406 174 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5949 Fragments arranged by size: 5949 683 56 BtsI GCAGTG_nn' Cuts at: 3312 4204 4998 5018 3312 Size: 892 794 20 4982 Fragments arranged by size: 4982 892 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6688 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6653 DraI TTT'AAA Cuts at: 3992 3992 Size: 6688 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5695 809 Size: 507 4379 1802 Fragments arranged by size: 4379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2105 3978 4135 Size: 54 1425 50 275 18 275 1873 157 Cuts at: 4135 5024 6653 8 Size: 889 1629 43 Fragments arranged by size: 1873 1629 1425 889 275 275 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4135 1537 Size: 275 2323 4090 Fragments arranged by size: 4090 2323 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2681 3893 4618 1353 Size: 1328 1212 725 3423 Fragments arranged by size: 3423 1328 1212 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5275 5649 5795 387 Size: 1064 138 3686 374 146 1280 Fragments arranged by size: 3686 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 3032 3034 3143 3488 3518 3573 Size: 393 425 975 2 109 345 30 55 Cuts at: 3573 3729 3756 3953 4751 5348 6135 6403 1239 Size: 156 27 197 798 597 787 268 1524 Fragments arranged by size: 1524 975 798 787 597 425 393 345 268 197 156 109 55 30 27 2 EcoNI CCTnn'n_nnAGG Cuts at: 2504 2504 Size: 6688 EcoO109I rG'GnC_Cy Cuts at: 2137 2320 2462 2715 2847 2983 3054 3055 3085 Size: 183 142 253 132 136 71 1 30 Cuts at: 3085 3584 3949 2137 Size: 499 365 4876 Fragments arranged by size: 4876 499 365 253 183 142 136 132 71 30 1 EcoRI G'AATT_C Cuts at: 3997 4375 3997 Size: 378 6310 FseI GG_CCGG'CC Cuts at: 2164 2164 Size: 6688 FspI TGC'GCA Cuts at: 5194 5194 Size: 6688 HaeII r_GCGC'y Cuts at: 1678 2603 3192 5835 1678 Size: 925 589 2643 2531 Fragments arranged by size: 2643 2531 925 589 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5329 5361 5403 5435 1373 Size: 32 135 32 3757 32 42 32 2626 Fragments arranged by size: 3757 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4032 4278 669 Size: 988 281 2094 246 3079 Fragments arranged by size: 3079 2094 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6688 HpaI GTT'AAC Cuts at: 4278 4278 Size: 6688 KasI G'GCGC_C Cuts at: 2599 3188 2599 Size: 589 6099 KpnI G_GTAC'C Cuts at: 4013 4013 Size: 6688 MfeI C'AATT_G Cuts at: 4287 4287 Size: 6688 MluI A'CGCG_T Cuts at: 6564 6564 Size: 6688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5802 5986 1305 Size: 4497 184 2007 Fragments arranged by size: 4497 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3980 10 Size: 54 1425 343 2148 2718 Fragments arranged by size: 2718 2148 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 2783 3701 4646 5005 5164 518 Size: 648 987 630 918 945 359 159 2042 Fragments arranged by size: 2042 987 945 918 648 630 359 159 MspA1I CmG'CkG Cuts at: 1606 2653 2689 2721 3007 3725 3959 4104 4781 Size: 1047 36 32 286 718 234 145 677 Cuts at: 4781 5929 6174 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1047 718 677 286 245 234 145 36 32 NaeI GCC'GGC Cuts at: 2162 2162 Size: 6688 NarI GG'CG_CC Cuts at: 2600 3189 2600 Size: 589 6099 NcoI C'CATG_G Cuts at: 513 1065 2700 3696 513 Size: 552 1635 996 3505 Fragments arranged by size: 3505 1635 996 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6688 NgoMIV G'CCGG_C Cuts at: 2160 2160 Size: 6688 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6688 NotI GC'GGCC_GC Cuts at: 4135 4135 Size: 6688 NspI r_CATG'y Cuts at: 1132 1229 3162 3463 4046 5591 1132 Size: 97 1933 301 583 1545 2229 Fragments arranged by size: 2229 1933 1545 583 301 97 PasI CC'CwG_GG Cuts at: 1348 2973 3580 1348 Size: 1625 607 4456 Fragments arranged by size: 4456 1625 607 PciI A'CATG_T Cuts at: 5587 5587 Size: 6688 PflMI CCAn_nnn'nTGG Cuts at: 1108 3252 1108 Size: 2144 4544 PfoI T'CCnGG_A Cuts at: 2046 3488 3930 2046 Size: 1442 442 4804 Fragments arranged by size: 4804 1442 442 PmeI GTTT'AAAC Cuts at: 3992 3992 Size: 6688 PmlI CAC'GTG Cuts at: 2542 2542 Size: 6688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4757 4789 6270 6302 4757 Size: 32 1481 32 5143 Fragments arranged by size: 5143 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2462 2983 3584 3949 2137 Size: 325 521 601 365 4876 Fragments arranged by size: 4876 601 521 365 325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6688 PsiI TTA'TAA Cuts at: 4258 4258 Size: 6688 PspOMI G'GGCC_C Cuts at: 2715 2846 3054 3084 2715 Size: 131 208 30 6319 Fragments arranged by size: 6319 208 131 30 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2216 2248 2216 Size: 32 6656 PstI C_TGCA'G Cuts at: 830 2583 3006 4040 830 Size: 1753 423 1034 3478 Fragments arranged by size: 3478 1753 1034 423 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6688 PvuII CAG'CTG Cuts at: 2689 3959 2689 Size: 1270 5418 SacII CC_GC'GG Cuts at: 2722 2722 Size: 6688 SalI G'TCGA_C Cuts at: 1936 4030 1936 Size: 2094 4594 SanDI GG'GwC_CC Cuts at: 2983 2983 Size: 6688 SapI GCTCTTCn'nnn_ Cuts at: 3893 3893 Size: 6688 SbfI CC_TGCA'GG Cuts at: 4040 4040 Size: 6688 ScaI AGT'ACT Cuts at: 4936 6648 4936 Size: 1712 4976 SfcI C'TryA_G Cuts at: 826 1045 2579 3002 4036 5171 5852 6043 826 Size: 219 1534 423 1034 1135 681 191 1471 Fragments arranged by size: 1534 1471 1135 1034 681 423 219 191 SfoI GGC'GCC Cuts at: 2601 3190 2601 Size: 589 6099 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6688 SmaI CCC'GGG Cuts at: 1872 2825 3371 4015 1872 Size: 953 546 644 4545 Fragments arranged by size: 4545 953 644 546 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 2324 3872 4792 5693 5955 Size: 197 926 141 240 1548 920 901 262 Cuts at: 5955 820 Size: 1553 Fragments arranged by size: 1553 1548 926 920 901 262 240 197 141 SnaBI TAC'GTA Cuts at: 493 4479 493 Size: 3986 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6688 SphI G_CATG'C Cuts at: 1132 4046 1132 Size: 2914 3774 SspI AAT'ATT Cuts at: 5 52 4612 5 Size: 47 4560 2081 Fragments arranged by size: 4560 2081 47 StuI AGG'CCT Cuts at: 1517 3037 3594 3741 1517 Size: 1520 557 147 4464 Fragments arranged by size: 4464 1520 557 147 StyI C'CwwG_G Cuts at: 513 1065 2242 2700 3032 3696 4127 513 Size: 552 1177 458 332 664 431 3074 Fragments arranged by size: 3074 1177 664 552 458 431 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2503 2550 3399 3506 3821 4715 4732 Size: 1255 923 47 849 107 315 894 17 Cuts at: 4732 4885 5070 5515 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 923 894 849 445 315 185 153 107 47 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2563 4934 6646 Size: 274 80 33 51 1443 586 2371 1712 Cuts at: 6646 96 Size: 138 Fragments arranged by size: 2371 1712 1443 586 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2286 2318 6648 6680 2286 Size: 32 4330 32 2294 Fragments arranged by size: 4330 2294 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6688 XbaI T'CTAG_A Cuts at: 4024 4024 Size: 6688 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2707 2707 Size: 6688 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6688 XmaI C'CCGG_G Cuts at: 1870 2823 3369 4013 1870 Size: 953 546 644 4545 Fragments arranged by size: 4545 953 644 546 XmnI GAAnn'nnTTC Cuts at: 4817 4817 Size: 6688 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4496 276 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PspXI RsrII SacI SexAI SfiI SrfI SwaI