(Linear) (Six-base) MAPSORT of: KIEE1524.seq Check: 4674 from: 1 to: 1983 KIEE1524.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AccI GT'mk_AC Cuts at: 0 227 1983 Size: 227 1756 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 1983 Size: 52 1931 AflIII A'CryG_T Cuts at: 0 534 914 1983 Size: 534 380 1069 Fragments arranged by size: 1069 534 380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 769 1983 Size: 769 1214 AlwNI CAG_nnn'CTG Cuts at: 0 436 573 1005 1983 Size: 436 137 432 978 Fragments arranged by size: 978 436 432 137 ApaI G_GGCC'C Cuts at: 0 714 845 1053 1083 1983 Size: 714 131 208 30 900 Fragments arranged by size: 900 714 208 131 30 ApaLI G'TGCA_C Cuts at: 0 388 724 1983 Size: 388 336 1259 Fragments arranged by size: 1259 388 336 ApoI r'AATT_y Cuts at: 0 1550 1983 Size: 1550 433 AvaI C'yCGr_G Cuts at: 0 818 1364 1983 Size: 818 546 619 Fragments arranged by size: 818 619 546 AvrII C'CTAG_G Cuts at: 0 1027 1983 Size: 1027 956 BanI G'GyrC_C Cuts at: 0 293 594 814 1183 1354 1983 Size: 293 301 220 369 171 629 Fragments arranged by size: 629 369 301 293 220 171 BanII G_rGCy'C Cuts at: 0 269 607 714 845 1020 1053 1083 1710 Size: 269 338 107 131 175 33 30 627 Cuts at: 1710 1983 Size: 273 Fragments arranged by size: 627 338 273 269 175 131 107 33 30 BbeI G_GCGC'C Cuts at: 0 598 1187 1983 Size: 598 589 796 Fragments arranged by size: 796 598 589 BbvCI CC'TCA_GC Cuts at: 0 1625 1983 Size: 1625 358 BglI GCCn_nnn'nGGC Cuts at: 0 473 1983 Size: 473 1510 Bme1580I G_kGCm'C Cuts at: 0 392 478 714 728 819 845 873 1053 Size: 392 86 236 14 91 26 28 180 Cuts at: 1053 1083 1574 1983 Size: 30 491 409 Fragments arranged by size: 491 409 392 236 180 91 86 30 28 26 14 BmgBI CAC'GTC Cuts at: 0 915 1983 Size: 915 1068 BmrI ACTGGGnnnn_n' Cuts at: 0 54 231 407 424 493 571 610 1844 Size: 54 177 176 17 69 78 39 1234 Cuts at: 1844 1983 Size: 139 Fragments arranged by size: 1234 177 176 139 78 69 54 39 17 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 33 966 1983 Size: 33 933 1017 Fragments arranged by size: 1017 933 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1027 1029 1138 1483 1513 1568 1724 1751 Size: 1027 2 109 345 30 55 156 27 Cuts at: 1751 1948 1983 Size: 197 35 Fragments arranged by size: 1027 345 197 156 109 55 35 30 27 2 Bpu10I CC'TnA_GC Cuts at: 0 254 302 1625 1983 Size: 254 48 1323 358 Fragments arranged by size: 1323 358 254 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 304 1852 1983 Size: 100 204 1548 131 Fragments arranged by size: 1548 204 131 100 BsaI GGTCTCn'nnnn_ Cuts at: 0 725 1983 Size: 725 1258 BsaAI yAC'GTr Cuts at: 0 537 1983 Size: 537 1446 BsaHI Gr'CG_yC Cuts at: 0 595 760 1184 1983 Size: 595 165 424 799 Fragments arranged by size: 799 595 424 165 BsaWI w'CCGG_w Cuts at: 0 1859 1983 Size: 1859 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 281 311 1832 1862 1983 Size: 281 30 1521 30 121 Fragments arranged by size: 1521 281 121 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 97 1461 1613 1983 Size: 97 1364 152 370 Fragments arranged by size: 1364 370 152 97 BseYI C'CCAG_C Cuts at: 0 164 363 1076 1290 1431 1630 1983 Size: 164 199 713 214 141 199 353 Fragments arranged by size: 713 353 214 199 199 164 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 595 1983 Size: 532 63 1388 Fragments arranged by size: 1388 532 63 BsiEI CG_ry'CG Cuts at: 0 1098 1983 Size: 1098 885 BsiHKAI G_wGCw'C Cuts at: 0 110 260 392 530 728 1983 Size: 110 150 132 138 198 1255 Fragments arranged by size: 1255 198 150 138 132 110 BsmI GAATG_Cn' Cuts at: 0 1703 1983 Size: 1703 280 Bsp1286I G_dGCh'C Cuts at: 0 110 260 269 392 478 530 607 714 Size: 110 150 9 123 86 52 77 107 Cuts at: 714 728 819 845 873 1020 1053 1083 1574 Size: 14 91 26 28 147 33 30 491 Cuts at: 1574 1710 1983 Size: 136 273 Fragments arranged by size: 491 273 150 147 136 123 110 107 91 86 77 52 33 30 28 26 14 9 BspEI T'CCGG_A Cuts at: 0 1859 1983 Size: 1859 124 BspHI T'CATG_A Cuts at: 0 512 1983 Size: 512 1471 BsrFI r'CCGG_y Cuts at: 0 155 1097 1898 1983 Size: 155 942 801 85 Fragments arranged by size: 942 801 155 85 BssHII G'CGCG_C Cuts at: 0 616 1983 Size: 616 1367 BssSI C'ACGA_G Cuts at: 0 652 1983 Size: 652 1331 BstEII G'GTnAC_C Cuts at: 0 1204 1797 1983 Size: 1204 593 186 Fragments arranged by size: 1204 593 186 BstXI CCAn_nnnn'nTGG Cuts at: 0 780 1491 1983 Size: 780 711 492 Fragments arranged by size: 780 711 492 BstYI r'GATC_y Cuts at: 0 233 1983 Size: 233 1750 Bsu36I CC'TnA_GG Cuts at: 0 8 128 1189 1983 Size: 8 120 1061 794 Fragments arranged by size: 1061 794 120 8 BtgI C'CryG_G Cuts at: 0 289 695 714 888 1691 1983 Size: 289 406 19 174 803 292 Fragments arranged by size: 803 406 292 289 174 19 BtsI GCAGTG_nn' Cuts at: 0 1307 1983 Size: 1307 676 EaeI y'GGCC_r Cuts at: 0 100 1973 1983 Size: 100 1873 10 Fragments arranged by size: 1873 100 10 EarI CTCTTCn'nnn_ Cuts at: 0 676 1888 1983 Size: 676 1212 95 Fragments arranged by size: 1212 676 95 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 1027 1029 1138 1483 1513 1568 1724 Size: 52 975 2 109 345 30 55 156 Cuts at: 1724 1751 1948 1983 Size: 27 197 35 Fragments arranged by size: 975 345 197 156 109 55 52 35 30 27 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 499 1983 Size: 499 1484 EcoO109I rG'GnC_Cy Cuts at: 0 132 315 457 710 842 978 1049 1050 Size: 132 183 142 253 132 136 71 1 Cuts at: 1050 1080 1579 1944 1983 Size: 30 499 365 39 Fragments arranged by size: 499 365 253 183 142 136 132 132 71 39 30 1 FseI GG_CCGG'CC Cuts at: 0 159 1983 Size: 159 1824 HaeII r_GCGC'y Cuts at: 0 598 1187 1983 Size: 598 589 796 Fragments arranged by size: 796 598 589 KasI G'GCGC_C Cuts at: 0 594 1183 1983 Size: 594 589 800 Fragments arranged by size: 800 594 589 MscI TGG'CCA Cuts at: 0 1975 1983 Size: 1975 8 MslI CAynn'nnrTG Cuts at: 0 148 778 1696 1983 Size: 148 630 918 287 Fragments arranged by size: 918 630 287 148 MspA1I CmG'CkG Cuts at: 0 648 684 716 1002 1720 1954 1983 Size: 648 36 32 286 718 234 29 Fragments arranged by size: 718 648 286 234 36 32 29 NaeI GCC'GGC Cuts at: 0 157 1983 Size: 157 1826 NarI GG'CG_CC Cuts at: 0 595 1184 1983 Size: 595 589 799 Fragments arranged by size: 799 595 589 NcoI C'CATG_G Cuts at: 0 695 1691 1983 Size: 695 996 292 Fragments arranged by size: 996 695 292 NgoMIV G'CCGG_C Cuts at: 0 155 1983 Size: 155 1828 NspI r_CATG'y Cuts at: 0 1157 1458 1983 Size: 1157 301 525 Fragments arranged by size: 1157 525 301 PasI CC'CwG_GG Cuts at: 0 968 1575 1983 Size: 968 607 408 Fragments arranged by size: 968 607 408 PflMI CCAn_nnn'nTGG Cuts at: 0 1247 1983 Size: 1247 736 PfoI T'CCnGG_A Cuts at: 0 41 1483 1925 1983 Size: 41 1442 442 58 Fragments arranged by size: 1442 442 58 41 PmlI CAC'GTG Cuts at: 0 537 1983 Size: 537 1446 PpuMI rG'GwC_Cy Cuts at: 0 132 457 978 1579 1944 1983 Size: 132 325 521 601 365 39 Fragments arranged by size: 601 521 365 325 132 39 PspOMI G'GGCC_C Cuts at: 0 710 841 1049 1079 1983 Size: 710 131 208 30 904 Fragments arranged by size: 904 710 208 131 30 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 211 243 1983 Size: 211 32 1740 Fragments arranged by size: 1740 211 32 PstI C_TGCA'G Cuts at: 0 578 1001 1983 Size: 578 423 982 Fragments arranged by size: 982 578 423 PvuII CAG'CTG Cuts at: 0 684 1954 1983 Size: 684 1270 29 Fragments arranged by size: 1270 684 29 SacII CC_GC'GG Cuts at: 0 717 1983 Size: 717 1266 SanDI GG'GwC_CC Cuts at: 0 978 1983 Size: 978 1005 SapI GCTCTTCn'nnn_ Cuts at: 0 1888 1983 Size: 1888 95 SfcI C'TryA_G Cuts at: 0 574 997 1983 Size: 574 423 986 Fragments arranged by size: 986 574 423 SfoI GGC'GCC Cuts at: 0 596 1185 1983 Size: 596 589 798 Fragments arranged by size: 798 596 589 SmaI CCC'GGG Cuts at: 0 820 1366 1983 Size: 820 546 617 Fragments arranged by size: 820 617 546 SmlI C'TyrA_G Cuts at: 0 79 319 1867 1983 Size: 79 240 1548 116 Fragments arranged by size: 1548 240 116 79 StuI AGG'CCT Cuts at: 0 1032 1589 1736 1983 Size: 1032 557 147 247 Fragments arranged by size: 1032 557 247 147 StyI C'CwwG_G Cuts at: 0 237 695 1027 1691 1983 Size: 237 458 332 664 292 Fragments arranged by size: 664 458 332 292 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 498 545 1394 1501 1816 1983 Size: 498 47 849 107 315 167 Fragments arranged by size: 849 498 315 167 107 47 TatI w'GTAC_w Cuts at: 0 558 1983 Size: 558 1425 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 281 313 1983 Size: 281 32 1670 Fragments arranged by size: 1670 281 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 702 1983 Size: 702 1281 XmaI C'CCGG_G Cuts at: 0 818 1364 1983 Size: 818 546 619 Fragments arranged by size: 818 619 546 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrFI BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I FseI HaeII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacII SanDI SapI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AscI AseI AsiSI BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI BmtI BsaBI BsiWI BsmBI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI