(Circular) (Six-base) MAPSORT of: pFN21AE6714.seq Check: 5288 from: 1 to: 8257 pFN21AE6714 8257 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2314 2314 Size: 8257 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6067 278 Size: 53 83 186 936 4531 2468 Fragments arranged by size: 4531 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5600 1937 Size: 3663 4594 Acc65I G'GTAC_C Cuts at: 5578 5578 Size: 8257 AclI AA'CG_TT Cuts at: 1563 5556 6384 6757 1563 Size: 3993 828 373 3063 Fragments arranged by size: 3993 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2849 2906 3979 4839 6320 7704 7972 1632 Size: 1217 57 1073 860 1481 1384 268 1917 Fragments arranged by size: 1917 1481 1384 1217 1073 860 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8060 AflIII A'CryG_T Cuts at: 1203 7156 8133 1203 Size: 5953 977 1327 Fragments arranged by size: 5953 1327 977 AgeI A'CCGG_T Cuts at: 7088 7088 Size: 8257 AhdI GACnn_n'nnGTC Cuts at: 6986 6986 Size: 8257 AleI CACnn'nnGTG Cuts at: 1166 2057 3428 3626 3662 5075 1166 Size: 891 1371 198 36 1413 4348 Fragments arranged by size: 4348 1413 1371 891 198 36 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3104 5057 1184 Size: 1920 1953 4384 Fragments arranged by size: 4384 1953 1920 AlwNI CAG_nnn'CTG Cuts at: 2726 3221 4863 5438 5548 6000 7572 2726 Size: 495 1642 575 110 452 1572 3411 Fragments arranged by size: 3411 1642 1572 575 495 452 110 ApaI G_GGCC'C Cuts at: 4680 4680 Size: 8257 ApaLI G'TGCA_C Cuts at: 6314 7470 6314 Size: 1156 7101 ApoI r'AATT_y Cuts at: 1493 4148 4526 4853 5566 5792 5944 8053 1493 Size: 2655 378 327 713 226 152 2109 1697 Fragments arranged by size: 2655 2109 1697 713 378 327 226 152 AseI AT'TA_AT Cuts at: 160 1032 6811 160 Size: 872 5779 1606 Fragments arranged by size: 5779 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8257 AvaI C'yCGr_G Cuts at: 1870 1943 4983 5458 5582 1870 Size: 73 3040 475 124 4545 Fragments arranged by size: 4545 3040 475 124 73 AvrII C'CTAG_G Cuts at: 4373 4373 Size: 8257 BamHI G'GATC_C Cuts at: 2429 3055 3886 4600 5587 5954 2429 Size: 626 831 714 987 367 4732 Fragments arranged by size: 4732 987 831 714 626 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2358 2708 3342 4717 5197 Size: 325 217 627 571 350 634 1375 480 Cuts at: 5197 5578 7033 618 Size: 381 1455 1842 Fragments arranged by size: 1842 1455 1375 634 627 571 480 381 350 325 217 BanII G_rGCy'C Cuts at: 1391 2162 2465 3516 3732 3849 4680 5576 1391 Size: 771 303 1051 216 117 831 896 4072 Fragments arranged by size: 4072 1051 896 831 771 303 216 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3795 4185 4509 4827 928 Size: 971 1896 390 324 318 4358 Fragments arranged by size: 4358 1896 971 390 324 318 BbvCI CC'TCA_GC Cuts at: 3092 4554 4662 3092 Size: 1462 108 6687 Fragments arranged by size: 6687 1462 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6448 6482 1903 Size: 34 4511 34 3678 Fragments arranged by size: 4511 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6149 7359 6149 Size: 1210 7047 BclI T'GATC_A Cuts at: 1548 5050 5550 1548 Size: 3502 500 4255 Fragments arranged by size: 4255 3502 500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2631 2723 2814 Size: 107 122 71 631 605 959 92 91 Cuts at: 2814 6868 136 Size: 4054 1525 Fragments arranged by size: 4054 1525 959 631 605 122 107 92 91 71 BglII A'GATC_T Cuts at: 8252 8252 Size: 8257 BlpI GC'TnA_GC Cuts at: 2488 5674 2488 Size: 3186 5071 Bme1580I G_kGCm'C Cuts at: 1790 2266 2713 2810 3578 4680 4931 5457 6318 Size: 476 447 97 768 1102 251 526 861 Cuts at: 6318 7474 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1102 861 768 526 476 447 251 97 BmgBI CAC'GTC Cuts at: 1318 3289 1318 Size: 1971 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 2517 3209 Size: 416 53 83 399 24 663 437 692 Cuts at: 3209 4719 5222 5413 5988 6946 442 Size: 1510 503 191 575 958 1753 Fragments arranged by size: 1753 1510 958 692 663 575 503 437 416 399 191 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8257 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3000 4027 4998 5363 3000 Size: 1027 971 365 5894 Fragments arranged by size: 5894 1027 971 365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2479 2497 3827 4226 4364 5042 5299 Size: 935 305 18 1330 399 138 678 257 Cuts at: 5299 6917 1239 Size: 1618 2579 Fragments arranged by size: 2579 1618 1330 935 678 399 305 257 138 18 Bpu10I CC'TnA_GC Cuts at: 3092 4554 4662 3092 Size: 1462 108 6687 Fragments arranged by size: 6687 1462 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6382 7247 7545 6382 Size: 865 298 7094 Fragments arranged by size: 7094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2475 3746 6920 882 Size: 617 976 1271 3174 2219 Fragments arranged by size: 3174 2219 1271 976 617 BsaAI yAC'GTr Cuts at: 493 1204 6048 493 Size: 711 4844 2702 Fragments arranged by size: 4844 2702 711 BsaBI GATnn'nnATC Cuts at: 8251 8251 Size: 8257 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 2750 4548 6064 Size: 53 83 186 936 696 521 1798 1516 Cuts at: 6064 6446 7081 275 Size: 382 635 1451 Fragments arranged by size: 1798 1516 1451 936 696 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4740 6690 7088 7362 7509 7920 4281 Size: 459 1950 398 274 147 411 4618 Fragments arranged by size: 4618 1950 459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3386 3416 5015 5045 1178 Size: 30 165 30 1983 30 1599 30 4390 Fragments arranged by size: 4390 1983 1599 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4145 4234 4679 4990 5302 5476 1186 Size: 2959 89 445 311 312 174 3967 Fragments arranged by size: 3967 2959 445 312 311 174 89 BseYI C'CCAG_C Cuts at: 2123 2713 2969 3116 3149 3174 4490 5413 7460 Size: 590 256 147 33 25 1316 923 2047 Cuts at: 7460 7956 2123 Size: 496 2424 Fragments arranged by size: 2424 2047 1316 923 590 496 256 147 33 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2578 3336 3377 3424 3463 4749 5053 Size: 573 842 758 41 47 39 1286 304 Cuts at: 5053 1163 Size: 4367 Fragments arranged by size: 4367 1286 842 758 573 304 47 41 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5284 5707 6468 7496 1540 Size: 275 186 3283 423 761 1028 2301 Fragments arranged by size: 3283 2301 1028 761 423 275 186 BsiHKAI G_wGCw'C Cuts at: 2489 3621 4080 4982 6318 6403 7474 2489 Size: 1132 459 902 1336 85 1071 3272 Fragments arranged by size: 3272 1336 1132 1071 902 459 85 BsiWI C'GTAC_G Cuts at: 2407 2407 Size: 8257 BsmI GAATG_Cn' Cuts at: 2647 3268 5768 5861 2647 Size: 621 2500 93 5043 Fragments arranged by size: 5043 2500 621 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2266 2465 2489 2713 2810 3516 Size: 399 372 104 199 24 224 97 706 Cuts at: 3516 3578 3621 3732 3849 4080 4680 4931 4982 Size: 62 43 111 117 231 600 251 51 Cuts at: 4982 5457 5576 6318 6403 7474 1391 Size: 475 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 706 600 475 399 372 251 231 224 199 119 117 111 104 97 85 62 51 43 24 BspEI T'CCGG_A Cuts at: 7920 7920 Size: 8257 BspHI T'CATG_A Cuts at: 5322 6146 7876 5322 Size: 824 1730 5703 Fragments arranged by size: 5703 1730 824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2314 2630 5612 844 Size: 1470 316 2982 3489 Fragments arranged by size: 3489 2982 1470 316 BsrBI CCG'CTC Cuts at: 1394 1823 2221 6144 1394 Size: 429 398 3923 3507 Fragments arranged by size: 3923 3507 429 398 BsrDI GCAATG_nn' Cuts at: 65 1248 3047 4678 5338 6752 6926 65 Size: 1183 1799 1631 660 1414 174 1396 Fragments arranged by size: 1799 1631 1414 1396 1183 660 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5283 6901 7088 1700 Size: 110 3473 1618 187 2869 Fragments arranged by size: 3473 2869 1618 187 110 BsrGI T'GTAC_A Cuts at: 96 2603 3534 96 Size: 2507 931 4819 Fragments arranged by size: 4819 2507 931 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8257 BssSI C'ACGA_G Cuts at: 6317 7329 6317 Size: 1012 7245 BstAPI GCAn_nnn'nTGC Cuts at: 794 4257 794 Size: 3463 4794 BstBI TT'CG_AA Cuts at: 7061 7061 Size: 8257 BstEII G'GTnAC_C Cuts at: 1184 4006 1184 Size: 2822 5435 BstXI CCAn_nnnn'nTGG Cuts at: 3407 3430 3739 5225 3407 Size: 23 309 1486 6439 Fragments arranged by size: 6439 1486 309 23 BstYI r'GATC_y Cuts at: 1972 2429 3055 3396 3886 4311 4600 5587 5954 Size: 457 626 341 490 425 289 987 367 Cuts at: 5954 6339 6356 7808 7894 7906 8252 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 987 626 490 457 425 385 367 346 341 289 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2719 2719 Size: 8257 BtgI C'CryG_G Cuts at: 513 1065 2004 2332 2465 3747 3795 4604 513 Size: 552 939 328 133 1282 48 809 4166 Fragments arranged by size: 4166 1282 939 809 552 328 133 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7518 Fragments arranged by size: 7518 683 56 BtsI GCAGTG_nn' Cuts at: 2566 2584 3412 4515 5041 5773 6567 6587 2566 Size: 18 828 1103 526 732 794 20 4236 Fragments arranged by size: 4236 1103 828 794 732 526 20 18 ClaI AT'CG_AT Cuts at: 1554 4765 1554 Size: 3211 5046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5017 5052 529 Size: 35 4453 35 3734 Fragments arranged by size: 4453 3734 35 35 DraI TTT'AAA Cuts at: 5561 5561 Size: 8257 DraIII CAC_nnn'GTG Cuts at: 4727 4727 Size: 8257 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7264 809 Size: 507 5948 1802 Fragments arranged by size: 5948 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2320 2383 3432 Size: 54 1425 50 275 18 490 63 1049 Cuts at: 3432 3526 4638 4838 5262 5281 5488 5704 6593 Size: 94 1112 200 424 19 207 216 889 Cuts at: 6593 8222 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1112 1049 889 490 424 275 216 207 200 94 63 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 5281 5704 1537 Size: 275 3469 423 4090 Fragments arranged by size: 4090 3469 423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 2568 4707 5321 5443 6187 1353 Size: 739 476 2139 614 122 744 3423 Fragments arranged by size: 3423 2139 744 739 614 476 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4954 6844 7218 7364 387 Size: 1064 138 3365 1890 374 146 1280 Fragments arranged by size: 3365 1890 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2479 2497 2849 2906 3827 3979 Size: 393 542 305 18 352 57 921 152 Cuts at: 3979 4226 4364 4839 5042 5299 6320 6917 7704 Size: 247 138 475 203 257 1021 597 787 Cuts at: 7704 7972 1239 Size: 268 1524 Fragments arranged by size: 1524 1021 921 787 597 542 475 393 352 305 268 257 247 203 152 138 57 18 EcoNI CCTnn'n_nnAGG Cuts at: 5078 5430 5461 5078 Size: 352 31 7874 Fragments arranged by size: 7874 352 31 EcoO109I rG'GnC_Cy Cuts at: 4275 4377 4409 4419 4860 5251 4275 Size: 102 32 10 441 391 7281 Fragments arranged by size: 7281 441 391 102 32 10 EcoRI G'AATT_C Cuts at: 4853 5566 5944 4853 Size: 713 378 7166 Fragments arranged by size: 7166 713 378 FspI TGC'GCA Cuts at: 6763 6763 Size: 8257 HaeII r_GCGC'y Cuts at: 1678 5497 7404 1678 Size: 3819 1907 2531 Fragments arranged by size: 3819 2531 1907 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 2778 2810 3703 Size: 32 135 32 962 32 212 32 893 Cuts at: 3703 3735 3800 3832 3990 4022 4067 4099 4433 Size: 32 65 32 158 32 45 32 334 Cuts at: 4433 4448 4465 4480 5285 5297 5317 5329 6898 Size: 15 17 15 805 12 20 12 1569 Cuts at: 6898 6930 6972 7004 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1569 962 893 805 334 212 158 135 65 45 42 32 32 32 32 32 32 32 32 32 32 20 17 15 15 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3652 5146 5167 5601 5847 669 Size: 988 281 1714 1494 21 434 246 3079 Fragments arranged by size: 3079 1714 1494 988 434 281 246 21 HindIII A'AGCT_T Cuts at: 748 748 Size: 8257 HpaI GTT'AAC Cuts at: 5847 5847 Size: 8257 KpnI G_GTAC'C Cuts at: 5582 5582 Size: 8257 MfeI C'AATT_G Cuts at: 4542 5856 4542 Size: 1314 6943 MluI A'CGCG_T Cuts at: 8133 8133 Size: 8257 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3701 4392 4565 7371 7555 1305 Size: 2396 691 173 2806 184 2007 Fragments arranged by size: 2806 2396 2007 691 184 173 MscI TGG'CCA Cuts at: 10 64 1489 1832 2322 2385 3434 3528 4640 Size: 54 1425 343 490 63 1049 94 1112 Cuts at: 4640 4840 5490 10 Size: 200 650 2777 Fragments arranged by size: 2777 1425 1112 1049 650 490 343 200 94 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2939 3428 3626 3662 5075 5223 Size: 648 891 882 489 198 36 1413 148 Cuts at: 5223 6215 6574 6733 518 Size: 992 359 159 2042 Fragments arranged by size: 2042 1413 992 891 882 648 489 359 198 159 148 36 MspA1I CmG'CkG Cuts at: 1606 2377 2492 3178 3447 3454 3914 4661 5012 Size: 771 115 686 269 7 460 747 351 Cuts at: 5012 5100 5673 6350 7498 7743 1606 Size: 88 573 677 1148 245 2120 Fragments arranged by size: 2120 1148 771 747 686 677 573 460 351 269 245 115 88 7 NcoI C'CATG_G Cuts at: 513 1065 2004 2332 2465 3747 3795 513 Size: 552 939 328 133 1282 48 4975 Fragments arranged by size: 4975 1282 939 552 328 133 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 8257 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8257 NotI GC'GGCC_GC Cuts at: 5704 5704 Size: 8257 NspI r_CATG'y Cuts at: 1132 1229 2665 3982 4710 5615 7160 1132 Size: 97 1436 1317 728 905 1545 2229 Fragments arranged by size: 2229 1545 1436 1317 905 728 97 PasI CC'CwG_GG Cuts at: 1348 3769 5092 1348 Size: 2421 1323 4513 Fragments arranged by size: 4513 2421 1323 PciI A'CATG_T Cuts at: 7156 7156 Size: 8257 PflMI CCAn_nnn'nTGG Cuts at: 1108 2022 2298 1108 Size: 914 276 7067 Fragments arranged by size: 7067 914 276 PfoI T'CCnGG_A Cuts at: 2043 2701 3818 3882 2043 Size: 658 1117 64 6418 Fragments arranged by size: 6418 1117 658 64 PmeI GTTT'AAAC Cuts at: 5561 5561 Size: 8257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6326 6358 7839 7871 6326 Size: 32 1481 32 6712 Fragments arranged by size: 6712 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5251 5251 Size: 8257 PshAI GACnn'nnGTC Cuts at: 1613 2510 4061 1613 Size: 897 1551 5809 Fragments arranged by size: 5809 1551 897 PsiI TTA'TAA Cuts at: 5827 5827 Size: 8257 PspOMI G'GGCC_C Cuts at: 4676 4676 Size: 8257 PspXI vC'TCGA_Gb Cuts at: 5458 5458 Size: 8257 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3965 3997 3965 Size: 32 8225 PstI C_TGCA'G Cuts at: 830 3105 3642 5434 5609 830 Size: 2275 537 1792 175 3478 Fragments arranged by size: 3478 2275 1792 537 175 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8257 PvuII CAG'CTG Cuts at: 2492 3178 3447 3914 4661 5012 5100 2492 Size: 686 269 467 747 351 88 5649 Fragments arranged by size: 5649 747 686 467 351 269 88 SalI G'TCGA_C Cuts at: 1936 5599 1936 Size: 3663 4594 SapI GCTCTTCn'nnn_ Cuts at: 5321 5321 Size: 8257 SbfI CC_TGCA'GG Cuts at: 5609 5609 Size: 8257 ScaI AGT'ACT Cuts at: 3125 6505 8217 3125 Size: 3380 1712 3165 Fragments arranged by size: 3380 3165 1712 SexAI A'CCwGG_T Cuts at: 5139 5139 Size: 8257 SfcI C'TryA_G Cuts at: 826 1045 3101 3638 3829 5430 5605 6740 7421 Size: 219 2056 537 191 1601 175 1135 681 Cuts at: 7421 7612 826 Size: 191 1471 Fragments arranged by size: 2056 1601 1471 1135 681 537 219 191 191 175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8257 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8257 SmaI CCC'GGG Cuts at: 1872 5584 1872 Size: 3712 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5458 6361 7262 7524 820 Size: 197 926 3515 903 901 262 1553 Fragments arranged by size: 3515 1553 926 903 901 262 197 SnaBI TAC'GTA Cuts at: 493 6048 493 Size: 5555 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8257 SphI G_CATG'C Cuts at: 1132 5615 1132 Size: 4483 3774 SspI AAT'ATT Cuts at: 5 52 3898 6181 5 Size: 47 3846 2283 2081 Fragments arranged by size: 3846 2283 2081 47 StuI AGG'CCT Cuts at: 1517 2021 1517 Size: 504 7753 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2332 2465 3165 3426 3435 Size: 552 939 12 316 133 700 261 9 Cuts at: 3435 3747 3795 4373 4646 5202 5265 5443 5696 Size: 312 48 578 273 556 63 178 253 Cuts at: 5696 513 Size: 3074 Fragments arranged by size: 3074 939 700 578 556 552 316 312 273 261 253 178 133 63 48 12 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2014 2099 4738 6284 6301 6454 6639 Size: 1255 434 85 2639 1546 17 153 185 Cuts at: 6639 7084 325 Size: 445 1498 Fragments arranged by size: 2639 1546 1498 1255 445 434 185 153 85 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2603 2860 3123 Size: 274 80 33 51 1443 626 257 263 Cuts at: 3123 3534 5184 6503 8215 96 Size: 411 1650 1319 1712 138 Fragments arranged by size: 1712 1650 1443 1319 626 411 274 263 257 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3386 3418 3535 3567 5015 5047 8217 8249 3386 Size: 32 117 32 1448 32 3170 32 3394 Fragments arranged by size: 3394 3170 1448 117 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4397 1316 Size: 3081 5176 XbaI T'CTAG_A Cuts at: 5593 5593 Size: 8257 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2330 2472 3146 3802 5498 2330 Size: 142 674 656 1696 5089 Fragments arranged by size: 5089 1696 674 656 142 XhoI C'TCGA_G Cuts at: 1943 5458 1943 Size: 3515 4742 XmaI C'CCGG_G Cuts at: 1870 5582 1870 Size: 3712 4545 XmnI GAAnn'nnTTC Cuts at: 6386 6386 Size: 8257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6065 276 Size: 53 83 186 936 4531 2468 Fragments arranged by size: 4531 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI