Restriction map for
pFN21AE6714
(Linear) (Six-base) MAPSORT of: KIEE6714.seq Check: 8824 from: 1 to: 3552
KIEE6714.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 309 3552
Size: 309 3243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 901 1974 2834 3552
Size: 844 57 1073 860 718
Fragments arranged by size:
1073 860 844 718 57
AleI CACnn'nnGTG
Cuts at: 0 52 1423 1621 1657 3070 3552
Size: 52 1371 198 36 1413 482
Fragments arranged by size:
1413 1371 482 198 52 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1099 3052 3552
Size: 1099 1953 500
Fragments arranged by size:
1953 1099 500
AlwNI CAG_nnn'CTG
Cuts at: 0 721 1216 2858 3433 3543 3552
Size: 721 495 1642 575 110 9
Fragments arranged by size:
1642 721 575 495 110 9
ApaI G_GGCC'C
Cuts at: 0 2675 3552
Size: 2675 877
ApoI r'AATT_y
Cuts at: 0 2143 2521 2848 3552
Size: 2143 378 327 704
Fragments arranged by size:
2143 704 378 327
AvaI C'yCGr_G
Cuts at: 0 2978 3453 3552
Size: 2978 475 99
Fragments arranged by size:
2978 475 99
AvrII C'CTAG_G
Cuts at: 0 2368 3552
Size: 2368 1184
BamHI G'GATC_C
Cuts at: 0 424 1050 1881 2595 3552
Size: 424 626 831 714 957
Fragments arranged by size:
957 831 714 626 424
BanI G'GyrC_C
Cuts at: 0 353 703 1337 2712 3192 3552
Size: 353 350 634 1375 480 360
Fragments arranged by size:
1375 634 480 360 353 350
BanII G_rGCy'C
Cuts at: 0 157 460 1511 1727 1844 2675 3552
Size: 157 303 1051 216 117 831 877
Fragments arranged by size:
1051 877 831 303 216 157 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 1790 2180 2504 2822 3552
Size: 1790 390 324 318 730
Fragments arranged by size:
1790 730 390 324 318
BbvCI CC'TCA_GC
Cuts at: 0 1087 2549 2657 3552
Size: 1087 1462 108 895
Fragments arranged by size:
1462 1087 895 108
BclI T'GATC_A
Cuts at: 0 3045 3545 3552
Size: 3045 500 7
Fragments arranged by size:
3045 500 7
BglI GCCn_nnn'nGGC
Cuts at: 0 626 718 809 3552
Size: 626 92 91 2743
Fragments arranged by size:
2743 626 92 91
BlpI GC'TnA_GC
Cuts at: 0 483 3552
Size: 483 3069
Bme1580I G_kGCm'C
Cuts at: 0 261 708 805 1573 2675 2926 3452 3552
Size: 261 447 97 768 1102 251 526 100
Fragments arranged by size:
1102 768 526 447 261 251 100 97
BmgBI CAC'GTC
Cuts at: 0 1284 3552
Size: 1284 2268
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 512 1204 2714 3217 3408 3552
Size: 75 437 692 1510 503 191 144
Fragments arranged by size:
1510 692 503 437 191 144 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 995 2022 2993 3358 3552
Size: 995 1027 971 365 194
Fragments arranged by size:
1027 995 971 365 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 474 492 1822 2221 2359 3037 3294
Size: 169 305 18 1330 399 138 678 257
Cuts at: 3294 3552
Size: 258
Fragments arranged by size:
1330 678 399 305 258 257 169 138
18
Bpu10I CC'TnA_GC
Cuts at: 0 1087 2549 2657 3552
Size: 1087 1462 108 895
Fragments arranged by size:
1462 1087 895 108
BsaI GGTCTCn'nnnn_
Cuts at: 0 470 1741 3552
Size: 470 1271 1811
Fragments arranged by size:
1811 1271 470
BsaHI Gr'CG_yC
Cuts at: 0 224 745 2543 3552
Size: 224 521 1798 1009
Fragments arranged by size:
1798 1009 521 224
BsaWI w'CCGG_w
Cuts at: 0 2276 2735 3552
Size: 2276 459 817
Fragments arranged by size:
2276 817 459
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1381 1411 3010 3040 3552
Size: 1381 30 1599 30 512
Fragments arranged by size:
1599 1381 512 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2140 2229 2674 2985 3297 3471 3552
Size: 2140 89 445 311 312 174 81
Fragments arranged by size:
2140 445 312 311 174 89 81
BseYI C'CCAG_C
Cuts at: 0 118 708 964 1111 1144 1169 2485 3408
Size: 118 590 256 147 33 25 1316 923
Cuts at: 3408 3552
Size: 144
Fragments arranged by size:
1316 923 590 256 147 144 118 33
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1331 1372 1419 1458 2744 3048 3552
Size: 573 758 41 47 39 1286 304 504
Fragments arranged by size:
1286 758 573 504 304 47 41 39
BsiEI CG_ry'CG
Cuts at: 0 3279 3552
Size: 3279 273
BsiHKAI G_wGCw'C
Cuts at: 0 484 1616 2075 2977 3552
Size: 484 1132 459 902 575
Fragments arranged by size:
1132 902 575 484 459
BsiWI C'GTAC_G
Cuts at: 0 402 3552
Size: 402 3150
BsmI GAATG_Cn'
Cuts at: 0 642 1263 3552
Size: 642 621 2289
Fragments arranged by size:
2289 642 621
Bsp1286I G_dGCh'C
Cuts at: 0 157 261 460 484 708 805 1511 1573
Size: 157 104 199 24 224 97 706 62
Cuts at: 1573 1616 1727 1844 2075 2675 2926 2977 3452
Size: 43 111 117 231 600 251 51 475
Cuts at: 3452 3552
Size: 100
Fragments arranged by size:
706 600 475 251 231 224 199 157
117 111 104 100 97 62 51 43
24
BspHI T'CATG_A
Cuts at: 0 3317 3552
Size: 3317 235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 309 625 3552
Size: 309 316 2927
Fragments arranged by size:
2927 316 309
BsrBI CCG'CTC
Cuts at: 0 216 3552
Size: 216 3336
BsrDI GCAATG_nn'
Cuts at: 0 1042 2673 3333 3552
Size: 1042 1631 660 219
Fragments arranged by size:
1631 1042 660 219
BsrFI r'CCGG_y
Cuts at: 0 3278 3552
Size: 3278 274
BsrGI T'GTAC_A
Cuts at: 0 598 1529 3552
Size: 598 931 2023
Fragments arranged by size:
2023 931 598
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2252 3552
Size: 2252 1300
BstEII G'GTnAC_C
Cuts at: 0 2001 3552
Size: 2001 1551
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1402 1425 1734 3220 3552
Size: 1402 23 309 1486 332
Fragments arranged by size:
1486 1402 332 309 23
BstYI r'GATC_y
Cuts at: 0 424 1050 1391 1881 2306 2595 3552
Size: 424 626 341 490 425 289 957
Fragments arranged by size:
957 626 490 425 424 341 289
Bsu36I CC'TnA_GG
Cuts at: 0 714 3552
Size: 714 2838
BtgI C'CryG_G
Cuts at: 0 327 460 1742 1790 2599 3552
Size: 327 133 1282 48 809 953
Fragments arranged by size:
1282 953 809 327 133 48
BtsI GCAGTG_nn'
Cuts at: 0 561 579 1407 2510 3036 3552
Size: 561 18 828 1103 526 516
Fragments arranged by size:
1103 828 561 526 516 18
ClaI AT'CG_AT
Cuts at: 0 2760 3552
Size: 2760 792
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3012 3047 3552
Size: 3012 35 505
Fragments arranged by size:
3012 505 35
DraIII CAC_nnn'GTG
Cuts at: 0 2722 3552
Size: 2722 830
EaeI y'GGCC_r
Cuts at: 0 315 378 1427 1521 2633 2833 3257 3276
Size: 315 63 1049 94 1112 200 424 19
Cuts at: 3276 3483 3552
Size: 207 69
Fragments arranged by size:
1112 1049 424 315 207 200 94 69
63 19
EagI C'GGCC_G
Cuts at: 0 3276 3552
Size: 3276 276
EarI CTCTTCn'nnn_
Cuts at: 0 87 563 2702 3316 3438 3552
Size: 87 476 2139 614 122 114
Fragments arranged by size:
2139 614 476 122 114 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2949 3552
Size: 2949 603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 474 492 844 901 1822 1974 2221
Size: 169 305 18 352 57 921 152 247
Cuts at: 2221 2359 2834 3037 3294 3552
Size: 138 475 203 257 258
Fragments arranged by size:
921 475 352 305 258 257 247 203
169 152 138 57 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3073 3425 3456 3552
Size: 3073 352 31 96
Fragments arranged by size:
3073 352 96 31
EcoO109I rG'GnC_Cy
Cuts at: 0 2270 2372 2404 2414 2855 3246 3552
Size: 2270 102 32 10 441 391 306
Fragments arranged by size:
2270 441 391 306 102 32 10
EcoRI G'AATT_C
Cuts at: 0 2848 3552
Size: 2848 704
HaeII r_GCGC'y
Cuts at: 0 3492 3552
Size: 3492 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 773 805 1698 1730 1795 1827
Size: 529 32 212 32 893 32 65 32
Cuts at: 1827 1985 2017 2062 2094 2428 2443 2460 2475
Size: 158 32 45 32 334 15 17 15
Cuts at: 2475 3280 3292 3312 3324 3552
Size: 805 12 20 12 228
Fragments arranged by size:
893 805 529 334 228 212 158 65
45 32 32 32 32 32 32 20
17 15 15 12 12
HincII GTy'rAC
Cuts at: 0 1647 3141 3162 3552
Size: 1647 1494 21 390
Fragments arranged by size:
1647 1494 390 21
MfeI C'AATT_G
Cuts at: 0 2537 3552
Size: 2537 1015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1696 2387 2560 3552
Size: 1696 691 173 992
Fragments arranged by size:
1696 992 691 173
MscI TGG'CCA
Cuts at: 0 317 380 1429 1523 2635 2835 3485 3552
Size: 317 63 1049 94 1112 200 650 67
Fragments arranged by size:
1112 1049 650 317 200 94 67 63
MslI CAynn'nnrTG
Cuts at: 0 52 934 1423 1621 1657 3070 3218 3552
Size: 52 882 489 198 36 1413 148 334
Fragments arranged by size:
1413 882 489 334 198 148 52 36
MspA1I CmG'CkG
Cuts at: 0 372 487 1173 1442 1449 1909 2656 3007
Size: 372 115 686 269 7 460 747 351
Cuts at: 3007 3095 3552
Size: 88 457
Fragments arranged by size:
747 686 460 457 372 351 269 115
88 7
NcoI C'CATG_G
Cuts at: 0 327 460 1742 1790 3552
Size: 327 133 1282 48 1762
Fragments arranged by size:
1762 1282 327 133 48
NspI r_CATG'y
Cuts at: 0 660 1977 2705 3552
Size: 660 1317 728 847
Fragments arranged by size:
1317 847 728 660
PasI CC'CwG_GG
Cuts at: 0 1764 3087 3552
Size: 1764 1323 465
Fragments arranged by size:
1764 1323 465
PflMI CCAn_nnn'nTGG
Cuts at: 0 17 293 3552
Size: 17 276 3259
Fragments arranged by size:
3259 276 17
PfoI T'CCnGG_A
Cuts at: 0 38 696 1813 1877 3552
Size: 38 658 1117 64 1675
Fragments arranged by size:
1675 1117 658 64 38
PpuMI rG'GwC_Cy
Cuts at: 0 3246 3552
Size: 3246 306
PshAI GACnn'nnGTC
Cuts at: 0 505 2056 3552
Size: 505 1551 1496
Fragments arranged by size:
1551 1496 505
PspOMI G'GGCC_C
Cuts at: 0 2671 3552
Size: 2671 881
PspXI vC'TCGA_Gb
Cuts at: 0 3453 3552
Size: 3453 99
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1960 1992 3552
Size: 1960 32 1560
Fragments arranged by size:
1960 1560 32
PstI C_TGCA'G
Cuts at: 0 1100 1637 3429 3552
Size: 1100 537 1792 123
Fragments arranged by size:
1792 1100 537 123
PvuII CAG'CTG
Cuts at: 0 487 1173 1442 1909 2656 3007 3095 3552
Size: 487 686 269 467 747 351 88 457
Fragments arranged by size:
747 686 487 467 457 351 269 88
SapI GCTCTTCn'nnn_
Cuts at: 0 3316 3552
Size: 3316 236
ScaI AGT'ACT
Cuts at: 0 1120 3552
Size: 1120 2432
SexAI A'CCwGG_T
Cuts at: 0 3134 3552
Size: 3134 418
SfcI C'TryA_G
Cuts at: 0 1096 1633 1824 3425 3552
Size: 1096 537 191 1601 127
Fragments arranged by size:
1601 1096 537 191 127
SmlI C'TyrA_G
Cuts at: 0 3453 3552
Size: 3453 99
SspI AAT'ATT
Cuts at: 0 1893 3552
Size: 1893 1659
StuI AGG'CCT
Cuts at: 0 16 3552
Size: 16 3536
StyI C'CwwG_G
Cuts at: 0 11 327 460 1160 1421 1430 1742 1790
Size: 11 316 133 700 261 9 312 48
Cuts at: 1790 2368 2641 3197 3260 3438 3552
Size: 578 273 556 63 178 114
Fragments arranged by size:
700 578 556 316 312 273 261 178
133 114 63 48 11 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 9 94 2733 3552
Size: 9 85 2639 819
Fragments arranged by size:
2639 819 85 9
TatI w'GTAC_w
Cuts at: 0 598 855 1118 1529 3179 3552
Size: 598 257 263 411 1650 373
Fragments arranged by size:
1650 598 411 373 263 257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1381 1413 1530 1562 3010 3042 3552
Size: 1381 32 117 32 1448 32 510
Fragments arranged by size:
1448 1381 510 117 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2392 3552
Size: 2392 1160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 325 467 1141 1797 3493 3552
Size: 325 142 674 656 1696 59
Fragments arranged by size:
1696 674 656 325 142 59
XhoI C'TCGA_G
Cuts at: 0 3453 3552
Size: 3453 99
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
ClaI CspCI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII MfeI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SapI
ScaI SexAI SfcI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AseI AsiSI BaeI BbeI
BcgI BciVI BfrBI BglII BmtI BpuEI BsaAI BsaBI
BsmBI BspEI BssHII BssSI BstBI BstZ17I BtgZI DraI
DrdI EcoICRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PsiI PvuI RsrII SacI SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI XbaI XmaI XmnI ZraI