Restriction map for
pFN21AB7143
##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI ApoI AscI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BcnI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MluI MroI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AluI 352 AGCT AG^CT
Alw44I 219 GTGCAC G^TGCAC
ApaLI 219 GTGCAC G^TGCAC
Asp700I 929 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 479 TGGCCA TGG^CCA
BanII 523 GAGCCC G[GA]GC[CT]^C
BbvCI 291 CCTCAGC CCTCAGC(-5/-2)
BglII 204 AGATCT A^GATCT
BsaJI 827 CCGTGG C^C[ACGT][ACGT]GG
BsaWI 359 TCCGGT [AT]^CCGG[AT]
BsiYI 982 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 982 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 843 GTCTC GTCTC(1/5)
BsmI 933 GCATTC GAATGC(1/-1)
BssKI 419 CCAGG ^CC[ACGT]GG
Bst1107I 178 GTATAC GTA^TAC
BstNI 419 CCAGG CC^[AT]GG
BstYI 204 AGATCT [GA]^GATC[CT]
BstZ17I 178 GTATAC GTA^TAC
DdeI 292 CTCAG C^T[ACGT]AG
DsaI 827 CCGTGG C^C[GA][CT]GG
EaeI 479 TGGCCA [CT]^GGCC[GA]
EcoRII 419 CCAGG ^CC[AT]GG
HapII 360 CCGG C^CGG
HincII 313 GTCAAC GT[CT]^[GA]AC
HindII 313 GTCAAC GT[CT]^[GA]AC
HinfI 802 GATTC G^A[ACGT]TC
HpaII 360 CCGG C^CGG
MaeII 756 ACGT A^CGT
MflI 204 AGATCT [GA]^GATC[CT]
MluNI 479 TGGCCA TGG^CCA
MscI 479 TGGCCA TGG^CCA
MslI 705 CATGGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 716 CCGCTG C[AC]G^C[GT]G
MspI 360 CCGG C^CGG
MvaI 419 CCAGG CC^[AT]GG
NspI 37 ACATGC [GA]CATG^[CT]
ScrFI 419 CCAGG CC^[ACGT]GG
SfcI 692 CTATAG C^T[GA][CT]AG
StuI 971 AGGCCT AGG^CCT
TfiI 802 GATTC G^A[AT]TC
TspRI 224 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 204 AGATCT [GA]^GATC[CT]
XmnI 929 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 178 GTATAC GT^[AC][GT]AC
918 GTCTAC
AseI 187 ATTAAT AT^TAAT
394 ATTAAT
AsnI 187 ATTAAT AT^TAAT
394 ATTAAT
AspHI 130 GTGCTC G[AT]GC[AT]^C
219 GTGCAC
BbvI 718 GCTGC GCAGC(8/12)
850 GCTGC
BclI 632 TGATCA T^GATCA
701 TGATCA
BsiHKAI 130 GTGCTC G[AT]GC[AT]^C
219 GTGCAC
BsrDI 366 CATTGC GCAATG(2/0)
119 GCAATG
BsrI 382 CCAGT ACTGG(1/-1)
461 CCAGT
FbaI 632 TGATCA T^GATCA
701 TGATCA
HaeIII 480 GGCC GG^CC
972 GGCC
HphI 274 GGTGA GGTGA(8/7)
765 TCACC
MfeI 371 CAATTG C^AATTG
858 CAATTG
MseI 188 TTAA T^TAA
395 TTAA
MunI 371 CAATTG C^AATTG
858 CAATTG
MwoI 110 GCTTCTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
287 GCCGCCTCAGC
PshBI 187 ATTAAT AT^TAAT
394 ATTAAT
SfaNI 329 GCATC GCATC(5/9)
853 GCATC
Tru9I 188 TTAA T^TAA
395 TTAA
TseI 718 GCTGC G^C[AT]GC
850 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AfaI BmyI Bsp1286I BstF5I
DpnII Fnu4HI FokI ItaI MaeIII
MboI MboII MnlI NdeII NlaIII
RsaI Sau3AI Tsp45I Tsp509I