(Linear) (Six-base) MAPSORT of: KIEE1391.seq Check: 244 from: 1 to: 2802 KIEE1391.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2605 2802 Size: 2605 197 AccI GT'mk_AC Cuts at: 0 1691 2802 Size: 1691 1111 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2034 2802 Size: 2034 768 AflIII A'CryG_T Cuts at: 0 1218 2802 Size: 1218 1584 AhdI GACnn_n'nnGTC Cuts at: 0 13 1613 2802 Size: 13 1600 1189 Fragments arranged by size: 1600 1189 13 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1556 2802 Size: 1556 1246 AlwNI CAG_nnn'CTG Cuts at: 0 1609 2344 2802 Size: 1609 735 458 Fragments arranged by size: 1609 735 458 ApaLI G'TGCA_C Cuts at: 0 1257 2802 Size: 1257 1545 ApoI r'AATT_y Cuts at: 0 427 2006 2244 2802 Size: 427 1579 238 558 Fragments arranged by size: 1579 558 427 238 AvaI C'yCGr_G Cuts at: 0 1757 2802 Size: 1757 1045 BamHI G'GATC_C Cuts at: 0 944 2802 Size: 944 1858 BanI G'GyrC_C Cuts at: 0 1636 2802 Size: 1636 1166 BanII G_rGCy'C Cuts at: 0 611 832 1384 2802 Size: 611 221 552 1418 Fragments arranged by size: 1418 611 552 221 BbsI GAAGACnn'nnnn_ Cuts at: 0 1405 1706 1832 2378 2802 Size: 1405 301 126 546 424 Fragments arranged by size: 1405 546 424 301 126 BbvCI CC'TCA_GC Cuts at: 0 1934 2802 Size: 1934 868 BciVI GTATCCnnnnn_n' Cuts at: 0 2032 2802 Size: 2032 770 BclI T'GATC_A Cuts at: 0 44 72 2802 Size: 44 28 2730 Fragments arranged by size: 2730 44 28 BfrBI ATG'CAT Cuts at: 0 82 380 2351 2802 Size: 82 298 1971 451 Fragments arranged by size: 1971 451 298 82 BglI GCCn_nnn'nGGC Cuts at: 0 2021 2201 2516 2802 Size: 2021 180 315 286 Fragments arranged by size: 2021 315 286 180 BglII A'GATC_T Cuts at: 0 310 990 2394 2802 Size: 310 680 1404 408 Fragments arranged by size: 1404 680 408 310 Bme1580I G_kGCm'C Cuts at: 0 1261 2802 Size: 1261 1541 BmrI ACTGGGnnnn_n' Cuts at: 0 320 2616 2802 Size: 320 2296 186 Fragments arranged by size: 2296 320 186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 662 1411 1652 1740 1931 2094 2348 2802 Size: 662 749 241 88 191 163 254 454 Fragments arranged by size: 749 662 454 254 241 191 163 88 Bpu10I CC'TnA_GC Cuts at: 0 1934 2802 Size: 1934 868 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 798 2802 Size: 798 2004 BsaWI w'CCGG_w Cuts at: 0 1964 2802 Size: 1964 838 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1371 1401 1713 1743 1985 2015 2401 2431 Size: 1371 30 312 30 242 30 386 30 Cuts at: 2431 2802 Size: 371 Fragments arranged by size: 1371 386 371 312 242 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 629 1408 1802 2802 Size: 629 779 394 1000 Fragments arranged by size: 1000 779 629 394 BseYI C'CCAG_C Cuts at: 0 2215 2322 2802 Size: 2215 107 480 Fragments arranged by size: 2215 480 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 979 1517 1815 2146 2802 Size: 979 538 298 331 656 Fragments arranged by size: 979 656 538 331 298 BsiEI CG_ry'CG Cuts at: 0 2520 2802 Size: 2520 282 BsiHKAI G_wGCw'C Cuts at: 0 832 1261 1384 1572 2802 Size: 832 429 123 188 1230 Fragments arranged by size: 1230 832 429 188 123 BsmI GAATG_Cn' Cuts at: 0 42 380 475 1768 2098 2353 2802 Size: 42 338 95 1293 330 255 449 Fragments arranged by size: 1293 449 338 330 255 95 42 Bsp1286I G_dGCh'C Cuts at: 0 611 832 1261 1384 1572 2802 Size: 611 221 429 123 188 1230 Fragments arranged by size: 1230 611 429 221 188 123 BspEI T'CCGG_A Cuts at: 0 1964 2802 Size: 1964 838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2605 2802 Size: 2605 197 BsrDI GCAATG_nn' Cuts at: 0 452 480 588 2802 Size: 452 28 108 2214 Fragments arranged by size: 2214 452 108 28 BsrGI T'GTAC_A Cuts at: 0 567 1058 2802 Size: 567 491 1744 Fragments arranged by size: 1744 567 491 BstYI r'GATC_y Cuts at: 0 310 944 990 2341 2394 2802 Size: 310 634 46 1351 53 408 Fragments arranged by size: 1351 634 408 310 53 46 Bsu36I CC'TnA_GG Cuts at: 0 1519 2802 Size: 1519 1283 BtgI C'CryG_G Cuts at: 0 265 2802 Size: 265 2537 BtsI GCAGTG_nn' Cuts at: 0 1152 1505 2802 Size: 1152 353 1297 Fragments arranged by size: 1297 1152 353 DraI TTT'AAA Cuts at: 0 2471 2802 Size: 2471 331 EaeI y'GGCC_r Cuts at: 0 2 444 698 1587 2517 2802 Size: 2 442 254 889 930 285 Fragments arranged by size: 930 889 442 285 254 2 EagI C'GGCC_G Cuts at: 0 2517 2802 Size: 2517 285 EarI CTCTTCn'nnn_ Cuts at: 0 401 2802 Size: 401 2401 EcoICRI GAG'CTC Cuts at: 0 830 1382 2802 Size: 830 552 1420 Fragments arranged by size: 1420 830 552 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 662 1411 1652 1740 1931 2034 2094 2348 Size: 662 749 241 88 191 103 60 254 Cuts at: 2348 2802 Size: 454 Fragments arranged by size: 749 662 454 254 241 191 103 88 60 EcoO109I rG'GnC_Cy Cuts at: 0 2069 2802 Size: 2069 733 EcoRI G'AATT_C Cuts at: 0 2244 2802 Size: 2244 558 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 31 221 253 605 637 666 698 1371 Size: 31 190 32 352 32 29 32 673 Cuts at: 1371 1403 1583 1599 1615 1631 1648 1663 1680 Size: 32 180 16 16 16 17 15 17 Cuts at: 1680 1695 2401 2433 2802 Size: 15 706 32 369 Fragments arranged by size: 706 673 369 352 190 180 32 32 32 32 32 31 29 17 17 16 16 16 15 15 HindIII A'AGCT_T Cuts at: 0 1526 2802 Size: 1526 1276 MluI A'CGCG_T Cuts at: 0 1218 2802 Size: 1218 1584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1971 2016 2444 2585 2802 Size: 1971 45 428 141 217 Fragments arranged by size: 1971 428 217 141 45 MscI TGG'CCA Cuts at: 0 4 2802 Size: 4 2798 MslI CAynn'nnrTG Cuts at: 0 2356 2802 Size: 2356 446 MspA1I CmG'CkG Cuts at: 0 101 449 2322 2802 Size: 101 348 1873 480 Fragments arranged by size: 1873 480 348 101 NcoI C'CATG_G Cuts at: 0 265 2802 Size: 265 2537 NsiI A_TGCA'T Cuts at: 0 84 382 2353 2802 Size: 84 298 1971 449 Fragments arranged by size: 1971 449 298 84 NspI r_CATG'y Cuts at: 0 2237 2802 Size: 2237 565 PasI CC'CwG_GG Cuts at: 0 1110 2802 Size: 1110 1692 PflMI CCAn_nnn'nTGG Cuts at: 0 1954 2802 Size: 1954 848 PfoI T'CCnGG_A Cuts at: 0 1717 2248 2784 2802 Size: 1717 531 536 18 Fragments arranged by size: 1717 536 531 18 PpuMI rG'GwC_Cy Cuts at: 0 2069 2802 Size: 2069 733 PshAI GACnn'nnGTC Cuts at: 0 267 2802 Size: 267 2535 PsiI TTA'TAA Cuts at: 0 1676 1742 2802 Size: 1676 66 1060 Fragments arranged by size: 1676 1060 66 PstI C_TGCA'G Cuts at: 0 738 2802 Size: 738 2064 PvuII CAG'CTG Cuts at: 0 101 2322 2802 Size: 101 2221 480 Fragments arranged by size: 2221 480 101 SacI G_AGCT'C Cuts at: 0 832 1384 2802 Size: 832 552 1418 Fragments arranged by size: 1418 832 552 ScaI AGT'ACT Cuts at: 0 163 2047 2802 Size: 163 1884 755 Fragments arranged by size: 1884 755 163 SfcI C'TryA_G Cuts at: 0 734 2376 2802 Size: 734 1642 426 Fragments arranged by size: 1642 734 426 SmlI C'TyrA_G Cuts at: 0 813 2802 Size: 813 1989 SspI AAT'ATT Cuts at: 0 1070 2802 Size: 1070 1732 StyI C'CwwG_G Cuts at: 0 265 2802 Size: 265 2537 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2274 2802 Size: 2274 528 TatI w'GTAC_w Cuts at: 0 20 161 567 1058 2045 2802 Size: 20 141 406 491 987 757 Fragments arranged by size: 987 757 491 406 141 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1240 1272 1713 1745 1785 1817 2802 Size: 1240 32 441 32 40 32 985 Fragments arranged by size: 1240 985 441 40 32 32 32 XbaI T'CTAG_A Cuts at: 0 1038 2802 Size: 1038 1764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1473 2069 2802 Size: 1473 596 733 Fragments arranged by size: 1473 733 596 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrGI BstYI Bsu36I BtgI BtsI DraI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HindIII MluI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacI ScaI SfcI SmlI SspI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsiWI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI XmnI ZraI