Restriction map for
pFN21AE4570
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccIII AclI AgeI Aor51HI
AscI AseI AsnI AspI AvrII
BamHI BfaI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI Eco47III EcoO65I EcoRV
EcoT14I EcoT22I FseI HgaI HincII
HindII HindIII HpaI MaeI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
StyI SwaI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 386 GGTACC G^GTACC
AccI 2027 GTCTAC GT^[AC][GT]AC
AcsI 485 GAATTC [GA]^AATT[CT]
AcyI 23 GGCGCC G[GA]^CG[CT]C
AflII 1621 CTTAAG C^TTAAG
AflIII 13 ACGTGT A^C[GA][CT]GT
AhdI 1894 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1596 GTGCAC G^TGCAC
AocI 1251 CCTGAGG CC^T[ACGT]AGG
ApaLI 1596 GTGCAC G^TGCAC
ApoI 485 GAATTC [GA]^AATT[CT]
Asp700I 2124 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 386 GGTACC G^GTACC
AspEI 1894 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1310 TGCGCA TGC^GCA
BbeI 23 GGCGCC GGCGC^C
BciVI 364 GGATAC GTATCC(6/5)
BclI 326 TGATCA T^GATCA
BfrI 1621 CTTAAG C^TTAAG
BglII 1154 AGATCT A^GATCT
BsaHI 23 GGCGCC G[GA]^CG[CT]C
BsaWI 2021 TCCGGT [AT]^CCGG[AT]
BsmI 1168 GCATTC GAATGC(1/-1)
Bsp1407I 854 TGTACA T^GTACA
BsrBI 1912 GAGCGG CCGCTC(-3/-3)
BsrDI 2073 CATTGC GCAATG(2/0)
BsrGI 854 TGTACA T^GTACA
BssHII 126 GCGCGC G^CGCGC
BssSI 2112 CACGAG CACGAG(-5/-1)
BstXI 637 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1251 CCTGAGG CC^T[ACGT]AGG
DraIII 964 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 757 GACCCGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 749 CCGCGG C^C[GA][CT]GG
Eam1105I 1894 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 373 GAAGAG CTCTTC(1/4)
Eco81I 1251 CCTGAGG CC^T[ACGT]AGG
EcoRI 485 GAATTC G^AATTC
FbaI 326 TGATCA T^GATCA
FspI 1310 TGCGCA TGC^GCA
Hin1I 23 GGCGCC G[GA]^CG[CT]C
KasI 23 GGCGCC G^GCGCC
KpnI 386 GGTACC GGTAC^C
Ksp632I 373 GAAGAG CTCTTC(1/4)
KspI 749 CCGCGG CCGC^GG
NarI 23 GGCGCC GG^CGCC
PflMI 680 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1265 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 784 GAGCTC GAGCT^C
SacII 749 CCGCGG CCGC^GG
ScaI 1295 AGTACT AGT^ACT
SfoI 23 GGCGCC GGC^GCC
SmaI 1838 CCCGGG CCC^GGG
SspBI 854 TGTACA T^GTACA
Van91I 680 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1838 CCCGGG C^CCGGG
XmaI 1838 CCCGGG C^CCGGG
XmnI 2124 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 949 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1180 CAGGCTCTG
AvaI 1213 CCCGAG C^[CT]CG[GA]G
1838 CCCGGG
BalI 560 TGGCCA TGG^CCA
2187 TGGCCA
BbrPI 12 CACGTG CAC^GTG
337 CACGTG
BcgI 76 CGACTCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
995 GCATCAGCATCG
BsoBI 1213 CCCGAG C^[CT]CG[GA]G
1838 CCCGGG
BspHI 1242 TCATGA T^CATGA
1571 TCATGA
HaeII 23 GGCGCC [GA]GCGC^[CT]
1617 GGCGCT
MaeIII 1420 GTCAC ^GT[ACGT]AC
1847 GTGAC
MluNI 560 TGGCCA TGG^CCA
2187 TGGCCA
MscI 560 TGGCCA TGG^CCA
2187 TGGCCA
MseI 191 TTAA T^TAA
1622 TTAA
PmaCI 12 CACGTG CAC^GTG
337 CACGTG
PmlI 12 CACGTG CAC^GTG
337 CACGTG
RcaI 1242 TCATGA T^CATGA
1571 TCATGA
SbfI 114 CCTGCAGG CCTGCA^GG
1398 CCTGCAGG
SexAI 515 ACCTGGT A^CC[AT]GGT
1765 ACCTGGT
Sse8387I 114 CCTGCAGG CCTGCA^GG
1398 CCTGCAGG
TaqI 1015 TCGA T^CGA
2000 TCGA
TfiI 983 GATTC G^A[AT]TC
1091 GAATC
Tru9I 191 TTAA T^TAA
1622 TTAA
Tsp45I 1420 GTCAC ^GT[GC]AC
1847 GTGAC
Tsp509I 184 AATT ^AATT
486 AATT
TthHB8I 1015 TCGA T^CGA
2000 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BglI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TseI TspRI XhoII