(Circular) (Six-base) MAPSORT of: pFN21AE4570.seq Check: 9132 from: 1 to: 6901 pFN21AE4570 6901 bp With 182 enzymes: SgfI * March 28, 2012 14:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2698 2943 2698 Size: 245 6656 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4711 278 Size: 53 83 186 936 3175 2468 Fragments arranged by size: 3175 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4033 4244 1937 Size: 2096 211 4594 Fragments arranged by size: 4594 2096 211 Acc65I G'GTAC_C Cuts at: 2391 4222 2391 Size: 1831 5070 AclI AA'CG_TT Cuts at: 1563 5028 5401 1563 Size: 3465 373 3063 Fragments arranged by size: 3465 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3319 4964 6348 6616 1632 Size: 1687 1645 1384 268 1917 Fragments arranged by size: 1917 1687 1645 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3626 820 Size: 197 2609 4095 Fragments arranged by size: 4095 2609 197 AflIII A'CryG_T Cuts at: 1203 2018 5800 6777 1203 Size: 815 3782 977 1327 Fragments arranged by size: 3782 1327 977 815 AgeI A'CCGG_T Cuts at: 5732 5732 Size: 6901 AhdI GACnn_n'nnGTC Cuts at: 3904 5630 3904 Size: 1726 5175 AleI CACnn'nnGTG Cuts at: 1166 2692 2925 3593 1166 Size: 1526 233 668 4474 Fragments arranged by size: 4474 1526 668 233 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2039 2375 3152 1184 Size: 855 336 777 4933 Fragments arranged by size: 4933 855 777 336 AlwNI CAG_nnn'CTG Cuts at: 2959 3190 4644 6216 2959 Size: 231 1454 1572 3644 Fragments arranged by size: 3644 1572 1454 231 ApaI G_GGCC'C Cuts at: 2042 3063 4150 2042 Size: 1021 1087 4793 Fragments arranged by size: 4793 1087 1021 ApaLI G'TGCA_C Cuts at: 3601 4958 6114 3601 Size: 1357 1156 4388 Fragments arranged by size: 4388 1357 1156 ApoI r'AATT_y Cuts at: 1493 2490 4210 4436 4588 6697 1493 Size: 997 1720 226 152 2109 1697 Fragments arranged by size: 2109 1720 1697 997 226 152 AseI AT'TA_AT Cuts at: 160 1032 5455 160 Size: 872 4423 1606 Fragments arranged by size: 4423 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 AvaI C'yCGr_G Cuts at: 1870 1943 3218 3843 4226 1870 Size: 73 1275 625 383 4545 Fragments arranged by size: 4545 1275 625 383 73 BamHI G'GATC_C Cuts at: 4231 4598 4231 Size: 367 6534 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2391 2668 2694 3143 Size: 325 217 627 241 363 277 26 449 Cuts at: 3143 3207 3767 4222 5677 618 Size: 64 560 455 1455 1842 Fragments arranged by size: 1842 1455 627 560 455 449 363 325 277 241 217 64 26 BanII G_rGCy'C Cuts at: 1391 2042 2058 2236 2533 2793 3063 4150 4186 Size: 651 16 178 297 260 270 1087 36 Cuts at: 4186 4220 1391 Size: 34 4072 Fragments arranged by size: 4072 1087 651 297 270 260 178 36 34 16 BbeI G_GCGC'C Cuts at: 2032 2032 Size: 6901 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2766 3556 4081 928 Size: 971 867 790 525 3748 Fragments arranged by size: 3748 971 867 790 525 BbvCI CC'TCA_GC Cuts at: 2271 3378 3607 2271 Size: 1107 229 5565 Fragments arranged by size: 5565 1107 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2070 2104 2989 3023 5092 5126 1903 Size: 34 133 34 885 34 2069 34 3678 Fragments arranged by size: 3678 2069 885 133 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2363 4793 6003 2363 Size: 2430 1210 3261 Fragments arranged by size: 3261 2430 1210 BclI T'GATC_A Cuts at: 1548 2331 1548 Size: 783 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 3126 3185 Size: 107 122 71 631 605 365 1089 59 Cuts at: 3185 3389 5512 136 Size: 204 2123 1525 Fragments arranged by size: 2123 1525 1089 631 605 365 204 122 107 71 59 BglII A'GATC_T Cuts at: 3159 6896 3159 Size: 3737 3164 BlpI GC'TnA_GC Cuts at: 4318 4318 Size: 6901 Bme1580I G_kGCm'C Cuts at: 1790 2018 2042 2283 2671 2932 3063 3146 3210 Size: 228 24 241 388 261 131 83 64 Cuts at: 3210 3534 3605 3940 4150 4962 6118 1790 Size: 324 71 335 210 812 1156 2573 Fragments arranged by size: 2573 1156 812 388 335 324 261 241 228 210 131 83 71 64 24 BmgBI CAC'GTC Cuts at: 1318 2109 1318 Size: 791 6110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2674 3934 4632 Size: 416 53 83 399 24 1257 1260 698 Cuts at: 4632 5590 442 Size: 958 1753 Fragments arranged by size: 1753 1260 1257 958 698 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6901 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3106 3467 3622 3106 Size: 361 155 6385 Fragments arranged by size: 6385 361 155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3076 3560 3803 5561 1239 Size: 1837 484 243 1758 2579 Fragments arranged by size: 2579 1837 1758 484 243 Bpu10I CC'TnA_GC Cuts at: 2271 3378 3607 2271 Size: 1107 229 5565 Fragments arranged by size: 5565 1107 229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3215 4058 5026 5891 6189 3215 Size: 843 968 865 298 3927 Fragments arranged by size: 3927 968 865 843 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3509 3671 3844 5564 882 Size: 617 2010 162 173 1720 2219 Fragments arranged by size: 2219 2010 1720 617 173 162 BsaAI yAC'GTr Cuts at: 493 1204 2019 2257 2344 4692 493 Size: 711 815 238 87 2348 2702 Fragments arranged by size: 2702 2348 815 711 238 87 BsaBI GATnn'nnATC Cuts at: 6895 6895 Size: 6901 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 4708 5090 5725 Size: 53 83 186 936 496 2679 382 635 Cuts at: 5725 275 Size: 1451 Fragments arranged by size: 2679 1451 936 635 496 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4026 5334 5732 6006 6153 6564 4026 Size: 1308 398 274 147 411 4363 Fragments arranged by size: 4363 1308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 2298 2328 1178 Size: 30 165 30 600 30 265 30 5751 Fragments arranged by size: 5751 600 265 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 3929 4035 1186 Size: 1705 1038 106 4052 Fragments arranged by size: 4052 1705 1038 106 BseYI C'CCAG_C Cuts at: 2424 2616 3888 3963 4082 4164 6104 6600 2424 Size: 192 1272 75 119 82 1940 496 2725 Fragments arranged by size: 2725 1940 1272 496 192 119 82 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2417 2426 2972 3355 3852 3950 1163 Size: 573 681 9 546 383 497 98 4114 Fragments arranged by size: 4114 681 573 546 497 383 98 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2074 2140 2986 4156 4351 5112 Size: 275 186 73 66 846 1170 195 761 Cuts at: 5112 6140 1540 Size: 1028 2301 Fragments arranged by size: 2301 1170 1028 846 761 275 195 186 73 66 BsiHKAI G_wGCw'C Cuts at: 2793 2919 3435 3605 3657 4962 5047 6118 2793 Size: 126 516 170 52 1305 85 1071 3576 Fragments arranged by size: 3576 1305 1071 516 170 126 85 52 BsmI GAATG_Cn' Cuts at: 3173 4412 4505 3173 Size: 1239 93 5569 Fragments arranged by size: 5569 1239 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2042 2058 2236 2283 2533 2671 Size: 399 228 24 16 178 47 250 138 Cuts at: 2671 2793 2919 2932 3063 3146 3210 3435 3534 Size: 122 126 13 131 83 64 225 99 Cuts at: 3534 3605 3657 3940 4150 4186 4220 4962 5047 Size: 71 52 283 210 36 34 742 85 Cuts at: 5047 6118 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 250 228 225 210 178 138 131 126 122 99 85 83 71 64 52 47 36 34 24 16 13 BspEI T'CCGG_A Cuts at: 6564 6564 Size: 6901 BspHI T'CATG_A Cuts at: 3247 3576 4790 6520 3247 Size: 329 1214 1730 3628 Fragments arranged by size: 3628 1730 1214 329 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2403 2698 2724 2943 4256 844 Size: 1283 276 295 26 219 1313 3489 Fragments arranged by size: 3489 1313 1283 295 276 219 26 BsrBI CCG'CTC Cuts at: 1394 1823 3919 4788 1394 Size: 429 2096 869 3507 Fragments arranged by size: 3507 2096 869 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4077 5396 5570 65 Size: 1183 2829 1319 174 1396 Fragments arranged by size: 2829 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 2135 2150 2671 2703 5545 5732 Size: 110 290 35 15 521 32 2842 187 Cuts at: 5732 1700 Size: 2869 Fragments arranged by size: 2869 2842 521 290 187 110 35 32 15 BsrGI T'GTAC_A Cuts at: 96 2859 96 Size: 2763 4138 BssHII G'CGCG_C Cuts at: 1925 2131 1925 Size: 206 6695 BssSI C'ACGA_G Cuts at: 4117 4961 5973 4117 Size: 844 1012 5045 Fragments arranged by size: 5045 1012 844 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6901 BstBI TT'CG_AA Cuts at: 5705 5705 Size: 6901 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6901 BstXI CCAn_nnnn'nTGG Cuts at: 2649 2649 Size: 6901 BstYI r'GATC_y Cuts at: 1972 3159 3669 3948 4231 4598 4983 5000 6452 Size: 1187 510 279 283 367 385 17 1452 Cuts at: 6452 6538 6550 6896 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1187 510 385 367 346 283 279 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3257 3257 Size: 6901 BtgI C'CryG_G Cuts at: 513 1065 2754 513 Size: 552 1689 4660 Fragments arranged by size: 4660 1689 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3471 487 Size: 683 56 2245 3917 Fragments arranged by size: 3917 2245 683 56 BtsI GCAGTG_nn' Cuts at: 2698 3069 4417 5211 5231 2698 Size: 371 1348 794 20 4368 Fragments arranged by size: 4368 1348 794 371 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6901 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2440 2475 2536 2571 529 Size: 35 1876 35 61 35 4859 Fragments arranged by size: 4859 1876 61 35 35 35 DraI TTT'AAA Cuts at: 4205 4205 Size: 6901 DraIII CAC_nnn'GTG Cuts at: 2974 2974 Size: 6901 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2768 5908 809 Size: 507 1452 3140 1802 Fragments arranged by size: 3140 1802 1452 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2137 2565 Size: 54 1425 50 275 18 241 66 428 Cuts at: 2565 2976 3984 4064 4153 4192 4348 5237 6866 Size: 411 1008 80 89 39 156 889 1629 Cuts at: 6866 8 Size: 43 Fragments arranged by size: 1629 1425 1008 889 428 411 275 241 156 89 80 66 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2071 2137 4153 4348 1537 Size: 275 259 66 2016 195 4090 Fragments arranged by size: 4090 2016 275 259 195 66 EarI CTCTTCn'nnn_ Cuts at: 1353 2373 4831 1353 Size: 1020 2458 3423 Fragments arranged by size: 3423 2458 1020 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2771 5488 5862 6008 387 Size: 1064 138 1182 2717 374 146 1280 Fragments arranged by size: 2717 1280 1182 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2791 2791 Size: 6901 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3076 3319 3560 3803 4964 5561 6348 Size: 393 1444 243 241 243 1161 597 787 Cuts at: 6348 6616 1239 Size: 268 1524 Fragments arranged by size: 1524 1444 1161 787 597 393 268 243 243 241 EcoNI CCTnn'n_nnAGG Cuts at: 2783 3239 3686 2783 Size: 456 447 5998 Fragments arranged by size: 5998 456 447 EcoO109I rG'GnC_Cy Cuts at: 2038 2039 2428 3271 4147 2038 Size: 1 389 843 876 4792 Fragments arranged by size: 4792 876 843 389 1 EcoRI G'AATT_C Cuts at: 2490 4210 4588 2490 Size: 1720 378 4803 Fragments arranged by size: 4803 1720 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2209 3343 2209 Size: 1134 5767 FspI TGC'GCA Cuts at: 3317 5407 3317 Size: 2090 4811 HaeII r_GCGC'y Cuts at: 1678 2032 3626 6048 1678 Size: 354 1594 2422 2531 Fragments arranged by size: 2531 2422 1594 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2432 2464 3212 3244 3890 Size: 32 135 32 860 32 748 32 646 Cuts at: 3890 3922 5542 5574 5616 5648 1373 Size: 32 1620 32 42 32 2626 Fragments arranged by size: 2626 1620 860 748 646 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4245 4491 669 Size: 988 281 2307 246 3079 Fragments arranged by size: 3079 2307 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6901 HpaI GTT'AAC Cuts at: 4491 4491 Size: 6901 KasI G'GCGC_C Cuts at: 2028 2028 Size: 6901 KpnI G_GTAC'C Cuts at: 2395 4226 2395 Size: 1831 5070 MfeI C'AATT_G Cuts at: 4500 4500 Size: 6901 MluI A'CGCG_T Cuts at: 6777 6777 Size: 6901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6015 6199 1305 Size: 4710 184 2007 Fragments arranged by size: 4710 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2567 4194 10 Size: 54 1425 343 735 1627 2717 Fragments arranged by size: 2717 1627 1425 735 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2692 2925 3032 3038 3134 3593 4859 Size: 648 1526 233 107 6 96 459 1266 Cuts at: 4859 5218 5377 518 Size: 359 159 2042 Fragments arranged by size: 2042 1526 1266 648 459 359 233 159 107 96 6 MspA1I CmG'CkG Cuts at: 1606 2076 2616 2756 2767 2874 2944 3665 4168 Size: 470 540 140 11 107 70 721 503 Cuts at: 4168 4317 4994 6142 6387 1606 Size: 149 677 1148 245 2120 Fragments arranged by size: 2120 1148 721 677 540 503 470 245 149 140 107 70 11 NaeI GCC'GGC Cuts at: 2102 2137 2705 2102 Size: 35 568 6298 Fragments arranged by size: 6298 568 35 NarI GG'CG_CC Cuts at: 2029 2029 Size: 6901 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6349 NdeI CA'TA_TG Cuts at: 387 387 Size: 6901 NgoMIV G'CCGG_C Cuts at: 2100 2135 2703 2100 Size: 35 568 6298 Fragments arranged by size: 6298 568 35 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6901 NotI GC'GGCC_GC Cuts at: 4348 4348 Size: 6901 NspI r_CATG'y Cuts at: 1132 1229 3037 3089 3963 4259 5804 1132 Size: 97 1808 52 874 296 1545 2229 Fragments arranged by size: 2229 1808 1545 874 296 97 52 PasI CC'CwG_GG Cuts at: 1348 2170 1348 Size: 822 6079 PciI A'CATG_T Cuts at: 5800 5800 Size: 6901 PflMI CCAn_nnn'nTGG Cuts at: 1108 2691 1108 Size: 1583 5318 PfoI T'CCnGG_A Cuts at: 3078 3099 3648 3672 3078 Size: 21 549 24 6307 Fragments arranged by size: 6307 549 24 21 PmeI GTTT'AAAC Cuts at: 4205 4205 Size: 6901 PmlI CAC'GTG Cuts at: 2019 2344 2019 Size: 325 6576 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4970 5002 6483 6515 4970 Size: 32 1481 32 5356 Fragments arranged by size: 5356 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3271 3271 Size: 6901 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6901 PsiI TTA'TAA Cuts at: 4471 4471 Size: 6901 PspOMI G'GGCC_C Cuts at: 2038 3059 4146 2038 Size: 1021 1087 4793 Fragments arranged by size: 4793 1087 1021 PstI C_TGCA'G Cuts at: 830 2124 2268 3240 3408 4253 830 Size: 1294 144 972 168 845 3478 Fragments arranged by size: 3478 1294 972 845 168 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6901 PvuII CAG'CTG Cuts at: 2616 2874 4168 2616 Size: 258 1294 5349 Fragments arranged by size: 5349 1294 258 SacI G_AGCT'C Cuts at: 2793 2793 Size: 6901 SacII CC_GC'GG Cuts at: 2757 2757 Size: 6901 SalI G'TCGA_C Cuts at: 1936 4243 1936 Size: 2307 4594 SbfI CC_TGCA'GG Cuts at: 2124 3408 4253 2124 Size: 1284 845 4772 Fragments arranged by size: 4772 1284 845 ScaI AGT'ACT Cuts at: 3302 5149 6861 3302 Size: 1847 1712 3342 Fragments arranged by size: 3342 1847 1712 SexAI A'CCwGG_T Cuts at: 2520 3770 2520 Size: 1250 5651 SfcI C'TryA_G Cuts at: 826 1045 2120 2264 3236 3404 4123 4249 5384 Size: 219 1075 144 972 168 719 126 1135 Cuts at: 5384 6065 6256 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1075 972 719 681 219 191 168 144 126 SfoI GGC'GCC Cuts at: 2030 2030 Size: 6901 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6901 SmaI CCC'GGG Cuts at: 1872 3845 4228 1872 Size: 1973 383 4545 Fragments arranged by size: 4545 1973 383 SmlI C'TyrA_G Cuts at: 820 1017 1943 3194 3626 4037 5005 5906 6168 Size: 197 926 1251 432 411 968 901 262 Cuts at: 6168 820 Size: 1553 Fragments arranged by size: 1553 1251 968 926 901 432 411 262 197 SnaBI TAC'GTA Cuts at: 493 4692 493 Size: 4199 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6901 SphI G_CATG'C Cuts at: 1132 4259 1132 Size: 3127 3774 SspI AAT'ATT Cuts at: 5 52 4825 5 Size: 47 4773 2081 Fragments arranged by size: 4773 2081 47 StuI AGG'CCT Cuts at: 1517 2325 2625 3383 3788 1517 Size: 808 300 758 405 4630 Fragments arranged by size: 4630 808 758 405 300 StyI C'CwwG_G Cuts at: 513 1065 4340 513 Size: 552 3275 3074 Fragments arranged by size: 3275 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 2486 3000 4928 4945 5098 5283 Size: 1255 486 420 514 1928 17 153 185 Cuts at: 5283 5728 325 Size: 445 1498 Fragments arranged by size: 1928 1498 1255 514 486 445 420 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 3300 5147 Size: 274 80 33 51 1443 882 441 1847 Cuts at: 5147 6859 96 Size: 1712 138 Fragments arranged by size: 1847 1712 1443 882 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2003 2035 2298 2330 3814 3846 6861 6893 2003 Size: 32 263 32 1484 32 3015 32 2011 Fragments arranged by size: 3015 2011 1484 263 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6901 XbaI T'CTAG_A Cuts at: 4237 4237 Size: 6901 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6901 XmaI C'CCGG_G Cuts at: 1870 3843 4226 1870 Size: 1973 383 4545 Fragments arranged by size: 4545 1973 383 XmnI GAAnn'nnTTC Cuts at: 4133 5030 4133 Size: 897 6004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4709 276 Size: 53 83 186 936 3175 2468 Fragments arranged by size: 3175 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI