Restriction map for
pFN21AE4570
(Linear) (Six-base) MAPSORT of: KIEE4570.seq Check: 9672 from: 1 to: 2196
KIEE4570.orf
With 182 enzymes: SgfI *
March 22, 2012 18:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 693 938 2196
Size: 693 245 1258
Fragments arranged by size:
1258 693 245
AccI GT'mk_AC
Cuts at: 0 2028 2196
Size: 2028 168
Acc65I G'GTAC_C
Cuts at: 0 386 2196
Size: 386 1810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1314 2196
Size: 1314 882
AflII C'TTAA_G
Cuts at: 0 1621 2196
Size: 1621 575
AflIII A'CryG_T
Cuts at: 0 13 2196
Size: 13 2183
AhdI GACnn_n'nnGTC
Cuts at: 0 1899 2196
Size: 1899 297
AleI CACnn'nnGTG
Cuts at: 0 687 920 1588 2196
Size: 687 233 668 608
Fragments arranged by size:
687 668 608 233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 370 1147 2196
Size: 34 336 777 1049
Fragments arranged by size:
1049 777 336 34
AlwNI CAG_nnn'CTG
Cuts at: 0 954 1185 2196
Size: 954 231 1011
Fragments arranged by size:
1011 954 231
ApaI G_GGCC'C
Cuts at: 0 37 1058 2145 2196
Size: 37 1021 1087 51
Fragments arranged by size:
1087 1021 51 37
ApaLI G'TGCA_C
Cuts at: 0 1596 2196
Size: 1596 600
ApoI r'AATT_y
Cuts at: 0 485 2196
Size: 485 1711
AvaI C'yCGr_G
Cuts at: 0 1213 1838 2196
Size: 1213 625 358
Fragments arranged by size:
1213 625 358
BanI G'GyrC_C
Cuts at: 0 23 386 663 689 1138 1202 1762 2196
Size: 23 363 277 26 449 64 560 434
Fragments arranged by size:
560 449 434 363 277 64 26 23
BanII G_rGCy'C
Cuts at: 0 37 53 231 528 788 1058 2145 2181
Size: 37 16 178 297 260 270 1087 36
Cuts at: 2181 2196
Size: 15
Fragments arranged by size:
1087 297 270 260 178 37 36 16
15
BbeI G_GCGC'C
Cuts at: 0 27 2196
Size: 27 2169
BbsI GAAGACnn'nnnn_
Cuts at: 0 761 1551 2076 2196
Size: 761 790 525 120
Fragments arranged by size:
790 761 525 120
BbvCI CC'TCA_GC
Cuts at: 0 266 1373 1602 2196
Size: 266 1107 229 594
Fragments arranged by size:
1107 594 266 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 65 99 984 1018 2196
Size: 65 34 885 34 1178
Fragments arranged by size:
1178 885 65 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 358 2196
Size: 358 1838
BclI T'GATC_A
Cuts at: 0 326 2196
Size: 326 1870
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1121 1180 1384 2196
Size: 32 1089 59 204 812
Fragments arranged by size:
1089 812 204 59 32
BglII A'GATC_T
Cuts at: 0 1154 2196
Size: 1154 1042
Bme1580I G_kGCm'C
Cuts at: 0 13 37 278 666 927 1058 1141 1205
Size: 13 24 241 388 261 131 83 64
Cuts at: 1205 1529 1600 1935 2145 2196
Size: 324 71 335 210 51
Fragments arranged by size:
388 335 324 261 241 210 131 83
71 64 51 24 13
BmgBI CAC'GTC
Cuts at: 0 104 2196
Size: 104 2092
BmrI ACTGGGnnnn_n'
Cuts at: 0 669 1929 2196
Size: 669 1260 267
Fragments arranged by size:
1260 669 267
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1101 1462 1617 2196
Size: 1101 361 155 579
Fragments arranged by size:
1101 579 361 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1555 1798 2196
Size: 1071 484 243 398
Fragments arranged by size:
1071 484 398 243
Bpu10I CC'TnA_GC
Cuts at: 0 266 1373 1602 2196
Size: 266 1107 229 594
Fragments arranged by size:
1107 594 266 229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 2053 2196
Size: 1210 843 143
Fragments arranged by size:
1210 843 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 1504 1666 1839 2196
Size: 1504 162 173 357
Fragments arranged by size:
1504 357 173 162
BsaAI yAC'GTr
Cuts at: 0 14 252 339 2196
Size: 14 238 87 1857
Fragments arranged by size:
1857 238 87 14
BsaHI Gr'CG_yC
Cuts at: 0 24 2196
Size: 24 2172
BsaWI w'CCGG_w
Cuts at: 0 2021 2196
Size: 2021 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 293 323 2196
Size: 28 265 30 1873
Fragments arranged by size:
1873 265 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 1924 2030 2196
Size: 886 1038 106 166
Fragments arranged by size:
1038 886 166 106
BseYI C'CCAG_C
Cuts at: 0 419 611 1883 1958 2077 2159 2196
Size: 419 192 1272 75 119 82 37
Fragments arranged by size:
1272 419 192 119 82 75 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 421 967 1350 1847 1945 2196
Size: 412 9 546 383 497 98 251
Fragments arranged by size:
546 497 412 383 251 98 9
BsiEI CG_ry'CG
Cuts at: 0 69 135 981 2151 2196
Size: 69 66 846 1170 45
Fragments arranged by size:
1170 846 69 66 45
BsiHKAI G_wGCw'C
Cuts at: 0 788 914 1430 1600 1652 2196
Size: 788 126 516 170 52 544
Fragments arranged by size:
788 544 516 170 126 52
BsmI GAATG_Cn'
Cuts at: 0 1168 2196
Size: 1168 1028
Bsp1286I G_dGCh'C
Cuts at: 0 13 37 53 231 278 528 666 788
Size: 13 24 16 178 47 250 138 122
Cuts at: 788 914 927 1058 1141 1205 1430 1529 1600
Size: 126 13 131 83 64 225 99 71
Cuts at: 1600 1652 1935 2145 2181 2196
Size: 52 283 210 36 15
Fragments arranged by size:
283 250 225 210 178 138 131 126
122 99 83 71 64 52 47 36
24 16 15 13 13
BspHI T'CATG_A
Cuts at: 0 1242 1571 2196
Size: 1242 329 625
Fragments arranged by size:
1242 625 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 398 693 719 938 2196
Size: 122 276 295 26 219 1258
Fragments arranged by size:
1258 295 276 219 122 26
BsrBI CCG'CTC
Cuts at: 0 1914 2196
Size: 1914 282
BsrDI GCAATG_nn'
Cuts at: 0 2072 2196
Size: 2072 124
BsrFI r'CCGG_y
Cuts at: 0 95 130 145 666 698 2196
Size: 95 35 15 521 32 1498
Fragments arranged by size:
1498 521 95 35 32 15
BsrGI T'GTAC_A
Cuts at: 0 854 2196
Size: 854 1342
BssHII G'CGCG_C
Cuts at: 0 126 2196
Size: 126 2070
BssSI C'ACGA_G
Cuts at: 0 2112 2196
Size: 2112 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 644 2196
Size: 644 1552
BstYI r'GATC_y
Cuts at: 0 1154 1664 1943 2196
Size: 1154 510 279 253
Fragments arranged by size:
1154 510 279 253
Bsu36I CC'TnA_GG
Cuts at: 0 1252 2196
Size: 1252 944
BtgI C'CryG_G
Cuts at: 0 749 2196
Size: 749 1447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1466 2196
Size: 1466 730
BtsI GCAGTG_nn'
Cuts at: 0 693 1064 2196
Size: 693 371 1132
Fragments arranged by size:
1132 693 371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 435 470 531 566 2196
Size: 435 35 61 35 1630
Fragments arranged by size:
1630 435 61 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 969 2196
Size: 969 1227
DrdI GACnn_nn'nnGTC
Cuts at: 0 763 2196
Size: 763 1433
EaeI y'GGCC_r
Cuts at: 0 66 132 560 971 1979 2059 2148 2187
Size: 66 66 428 411 1008 80 89 39
Cuts at: 2187 2196
Size: 9
Fragments arranged by size:
1008 428 411 89 80 66 66 39
9
EagI C'GGCC_G
Cuts at: 0 66 132 2148 2196
Size: 66 66 2016 48
Fragments arranged by size:
2016 66 66 48
EarI CTCTTCn'nnn_
Cuts at: 0 368 2196
Size: 368 1828
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 766 2196
Size: 766 1430
EcoICRI GAG'CTC
Cuts at: 0 786 2196
Size: 786 1410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1314 1555 1798 2196
Size: 1071 243 241 243 398
Fragments arranged by size:
1071 398 243 243 241
EcoNI CCTnn'n_nnAGG
Cuts at: 0 778 1234 1681 2196
Size: 778 456 447 515
Fragments arranged by size:
778 515 456 447
EcoO109I rG'GnC_Cy
Cuts at: 0 33 34 423 1266 2142 2196
Size: 33 1 389 843 876 54
Fragments arranged by size:
876 843 389 54 33 1
EcoRI G'AATT_C
Cuts at: 0 485 2196
Size: 485 1711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 1338 2196
Size: 204 1134 858
Fragments arranged by size:
1134 858 204
FspI TGC'GCA
Cuts at: 0 1312 2196
Size: 1312 884
HaeII r_GCGC'y
Cuts at: 0 27 1621 2196
Size: 27 1594 575
Fragments arranged by size:
1594 575 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 1207 1239 1885 1917 2196
Size: 427 32 748 32 646 32 279
Fragments arranged by size:
748 646 427 279 32 32 32
KasI G'GCGC_C
Cuts at: 0 23 2196
Size: 23 2173
KpnI G_GTAC'C
Cuts at: 0 390 2196
Size: 390 1806
MscI TGG'CCA
Cuts at: 0 562 2189 2196
Size: 562 1627 7
Fragments arranged by size:
1627 562 7
MslI CAynn'nnrTG
Cuts at: 0 687 920 1027 1033 1129 1588 2196
Size: 687 233 107 6 96 459 608
Fragments arranged by size:
687 608 459 233 107 96 6
MspA1I CmG'CkG
Cuts at: 0 71 611 751 762 869 939 1660 2163
Size: 71 540 140 11 107 70 721 503
Cuts at: 2163 2196
Size: 33
Fragments arranged by size:
721 540 503 140 107 71 70 33
11
NaeI GCC'GGC
Cuts at: 0 97 132 700 2196
Size: 97 35 568 1496
Fragments arranged by size:
1496 568 97 35
NarI GG'CG_CC
Cuts at: 0 24 2196
Size: 24 2172
NgoMIV G'CCGG_C
Cuts at: 0 95 130 698 2196
Size: 95 35 568 1498
Fragments arranged by size:
1498 568 95 35
NspI r_CATG'y
Cuts at: 0 1032 1084 1958 2196
Size: 1032 52 874 238
Fragments arranged by size:
1032 874 238 52
PasI CC'CwG_GG
Cuts at: 0 165 2196
Size: 165 2031
PflMI CCAn_nnn'nTGG
Cuts at: 0 686 2196
Size: 686 1510
PfoI T'CCnGG_A
Cuts at: 0 1073 1094 1643 1667 2196
Size: 1073 21 549 24 529
Fragments arranged by size:
1073 549 529 24 21
PmlI CAC'GTG
Cuts at: 0 14 339 2196
Size: 14 325 1857
Fragments arranged by size:
1857 325 14
PpuMI rG'GwC_Cy
Cuts at: 0 1266 2196
Size: 1266 930
PspOMI G'GGCC_C
Cuts at: 0 33 1054 2141 2196
Size: 33 1021 1087 55
Fragments arranged by size:
1087 1021 55 33
PstI C_TGCA'G
Cuts at: 0 119 263 1235 1403 2196
Size: 119 144 972 168 793
Fragments arranged by size:
972 793 168 144 119
PvuII CAG'CTG
Cuts at: 0 611 869 2163 2196
Size: 611 258 1294 33
Fragments arranged by size:
1294 611 258 33
SacI G_AGCT'C
Cuts at: 0 788 2196
Size: 788 1408
SacII CC_GC'GG
Cuts at: 0 752 2196
Size: 752 1444
SbfI CC_TGCA'GG
Cuts at: 0 119 1403 2196
Size: 119 1284 793
Fragments arranged by size:
1284 793 119
ScaI AGT'ACT
Cuts at: 0 1297 2196
Size: 1297 899
SexAI A'CCwGG_T
Cuts at: 0 515 1765 2196
Size: 515 1250 431
Fragments arranged by size:
1250 515 431
SfcI C'TryA_G
Cuts at: 0 115 259 1231 1399 2118 2196
Size: 115 144 972 168 719 78
Fragments arranged by size:
972 719 168 144 115 78
SfoI GGC'GCC
Cuts at: 0 25 2196
Size: 25 2171
SmaI CCC'GGG
Cuts at: 0 1840 2196
Size: 1840 356
SmlI C'TyrA_G
Cuts at: 0 1189 1621 2032 2196
Size: 1189 432 411 164
Fragments arranged by size:
1189 432 411 164
StuI AGG'CCT
Cuts at: 0 320 620 1378 1783 2196
Size: 320 300 758 405 413
Fragments arranged by size:
758 413 405 320 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 481 995 2196
Size: 61 420 514 1201
Fragments arranged by size:
1201 514 420 61
TatI w'GTAC_w
Cuts at: 0 854 1295 2196
Size: 854 441 901
Fragments arranged by size:
901 854 441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 293 325 1809 1841 2196
Size: 30 263 32 1484 32 355
Fragments arranged by size:
1484 355 263 32 32 30
XmaI C'CCGG_G
Cuts at: 0 1838 2196
Size: 1838 358
XmnI GAAnn'nnTTC
Cuts at: 0 2128 2196
Size: 2128 68
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I KasI KpnI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SbfI ScaI SexAI SfcI
SfoI SmaI SmlI StuI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AloI AscI AseI AsiSI
AvrII BaeI BamHI BfrBI BlpI BmtI BsaBI BsiWI
BsmBI BspEI BstAPI BstBI BstEII BstZ17I ClaI DraI
EcoRV FseI FspAI HincII HindIII HpaI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI
XcmI XhoI ZraI