(Circular) (Six-base) MAPSORT of: pFN21AE0921.seq Check: 3758 from: 1 to: 5551 pFN21AE0921 5551 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2039 2872 2039 Size: 833 4718 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3614 4998 5266 1632 Size: 1982 1384 268 1917 Fragments arranged by size: 1982 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 2213 4450 5427 1203 Size: 1010 2237 977 1327 Fragments arranged by size: 2237 1327 1010 977 AgeI A'CCGG_T Cuts at: 2036 4382 2036 Size: 2346 3205 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 3294 4866 3294 Size: 1572 3979 ApaI G_GGCC'C Cuts at: 2237 2237 Size: 5551 ApaLI G'TGCA_C Cuts at: 3608 4764 3608 Size: 1156 4395 ApoI r'AATT_y Cuts at: 1493 2662 2860 3086 3238 5347 1493 Size: 1169 198 226 152 2109 1697 Fragments arranged by size: 2109 1697 1169 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 2514 4105 160 Size: 872 1482 1591 1606 Fragments arranged by size: 1606 1591 1482 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2876 1870 Size: 73 933 4545 Fragments arranged by size: 4545 933 73 BamHI G'GATC_C Cuts at: 2243 2881 3248 2243 Size: 638 367 4546 Fragments arranged by size: 4546 638 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2068 2872 4327 618 Size: 325 217 627 252 29 804 1455 1842 Fragments arranged by size: 1842 1455 804 627 325 252 217 29 BanII G_rGCy'C Cuts at: 1391 2237 2364 2870 1391 Size: 846 127 506 4072 Fragments arranged by size: 4072 846 506 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 928 Size: 971 402 4178 Fragments arranged by size: 4178 971 402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3742 3776 1903 Size: 34 1805 34 3678 Fragments arranged by size: 3678 1805 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3443 4653 3443 Size: 1210 4341 BclI T'GATC_A Cuts at: 1548 1548 Size: 5551 BfrBI ATG'CAT Cuts at: 2452 2452 Size: 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4162 136 Size: 107 122 71 631 605 2490 1525 Fragments arranged by size: 2490 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5546 5546 Size: 5551 BlpI GC'TnA_GC Cuts at: 2968 2968 Size: 5551 Bme1580I G_kGCm'C Cuts at: 1790 2237 2396 3612 4768 1790 Size: 447 159 1216 1156 2573 Fragments arranged by size: 2573 1216 1156 447 159 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2545 3282 4240 Size: 416 53 83 399 24 1128 737 958 Cuts at: 4240 442 Size: 1753 Fragments arranged by size: 1753 1128 958 737 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4211 1239 Size: 2972 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 3676 4541 4839 2699 Size: 977 865 298 3411 Fragments arranged by size: 3411 977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4214 882 Size: 617 2715 2219 Fragments arranged by size: 2715 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3342 493 Size: 711 2138 2702 Fragments arranged by size: 2702 2138 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3358 3740 4375 275 Size: 53 83 186 936 1825 382 635 1451 Fragments arranged by size: 1825 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2036 3984 4382 4656 4803 5214 2036 Size: 1948 398 274 147 411 2373 Fragments arranged by size: 2373 1948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5326 Fragments arranged by size: 5326 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2712 1186 Size: 1526 4025 BseYI C'CCAG_C Cuts at: 4754 5250 4754 Size: 496 5055 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4978 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3762 4790 1540 Size: 275 186 1000 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 3612 3697 4768 2364 Size: 1248 85 1071 3147 Fragments arranged by size: 3147 1248 1071 85 BsmI GAATG_Cn' Cuts at: 3062 3155 3062 Size: 93 5458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2364 2396 2870 3612 3697 4768 Size: 399 447 127 32 474 742 85 1071 Cuts at: 4768 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 474 447 399 127 85 32 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 2734 3440 5170 2734 Size: 706 1730 3115 Fragments arranged by size: 3115 1730 706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2906 844 Size: 2062 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3438 1394 Size: 429 1615 3507 Fragments arranged by size: 3507 1615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2790 4046 4220 65 Size: 1183 1542 1256 174 1396 Fragments arranged by size: 1542 1396 1256 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 4195 4382 1700 Size: 110 226 2159 187 2869 Fragments arranged by size: 2869 2159 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5551 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5551 BssSI C'ACGA_G Cuts at: 3611 4623 3611 Size: 1012 4539 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5551 BstBI TT'CG_AA Cuts at: 4355 4355 Size: 5551 BstEII G'GTnAC_C Cuts at: 1184 2606 1184 Size: 1422 4129 BstYI r'GATC_y Cuts at: 1972 2243 2881 3248 3633 3650 5102 5188 5200 Size: 271 638 367 385 17 1452 86 12 Cuts at: 5200 5546 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 638 385 367 346 271 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4999 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4812 Fragments arranged by size: 4812 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2774 4558 809 Size: 507 1458 1784 1802 Fragments arranged by size: 1802 1784 1458 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2176 2998 3887 Size: 54 1425 50 275 18 346 822 889 Cuts at: 3887 5516 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 822 346 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 1537 Size: 275 1186 4090 Fragments arranged by size: 4090 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 3481 1353 Size: 999 1129 3423 Fragments arranged by size: 3423 1129 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4138 4512 4658 387 Size: 1064 138 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 5551 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3614 4211 4998 5266 1239 Size: 393 1982 597 787 268 1524 Fragments arranged by size: 1982 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2233 2234 2233 Size: 1 5550 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 4698 1678 Size: 3020 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 4192 4224 4266 Size: 32 135 32 592 32 1996 32 42 Cuts at: 4266 4298 1373 Size: 32 2626 Fragments arranged by size: 2626 1996 592 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2533 2895 3141 669 Size: 988 281 595 362 246 3079 Fragments arranged by size: 3079 988 595 362 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KpnI G_GTAC'C Cuts at: 2043 2876 2043 Size: 833 4718 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2246 4665 4849 1305 Size: 816 125 2419 184 2007 Fragments arranged by size: 2419 2007 816 184 125 MscI TGG'CCA Cuts at: 10 64 1489 1832 2178 10 Size: 54 1425 343 346 3383 Fragments arranged by size: 3383 1425 346 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3509 3868 4027 518 Size: 648 2343 359 159 2042 Fragments arranged by size: 2343 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2635 2967 3644 4792 5037 1606 Size: 1029 332 677 1148 245 2120 Fragments arranged by size: 2120 1148 1029 677 332 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4999 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NsiI A_TGCA'T Cuts at: 2454 2454 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2217 2909 4454 1132 Size: 97 988 692 1545 2229 Fragments arranged by size: 2229 1545 988 692 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5551 PciI A'CATG_T Cuts at: 2213 4450 2213 Size: 2237 3314 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5551 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3620 3652 5133 5165 3620 Size: 32 1481 32 4006 Fragments arranged by size: 4006 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PspOMI G'GGCC_C Cuts at: 2233 2233 Size: 5551 PstI C_TGCA'G Cuts at: 830 2903 830 Size: 2073 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 PvuII CAG'CTG Cuts at: 2635 2635 Size: 5551 SacI G_AGCT'C Cuts at: 2364 2364 Size: 5551 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SapI GCTCTTCn'nnn_ Cuts at: 2352 2352 Size: 5551 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 2286 3799 5511 2286 Size: 1513 1712 2326 Fragments arranged by size: 2326 1712 1513 SfcI C'TryA_G Cuts at: 826 1045 2022 2477 2505 2899 4034 4715 4906 Size: 219 977 455 28 394 1135 681 191 Cuts at: 4906 826 Size: 1471 Fragments arranged by size: 1471 1135 977 681 455 394 219 191 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5551 SmaI CCC'GGG Cuts at: 1872 2878 1872 Size: 1006 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 3655 4556 4818 820 Size: 197 926 735 977 901 262 1553 Fragments arranged by size: 1553 977 926 901 735 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 2377 1517 Size: 860 4691 StyI C'CwwG_G Cuts at: 513 1065 2990 513 Size: 552 1925 3074 Fragments arranged by size: 3074 1925 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2527 3578 3595 3748 3933 4378 325 Size: 1255 947 1051 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1051 947 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2284 3797 5509 Size: 274 80 33 51 1443 307 1513 1712 Cuts at: 5509 96 Size: 138 Fragments arranged by size: 1712 1513 1443 307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2203 2235 5511 5543 2203 Size: 32 3276 32 2211 Fragments arranged by size: 3276 2211 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2104 2254 2104 Size: 150 5401 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2876 1870 Size: 1006 4545 XmnI GAAnn'nnTTC Cuts at: 3680 3680 Size: 5551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI