################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MfeI MflI MluI MroI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StyI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 925 GACGTC GACGT^C BbvCI 1038 CCTCAGC CCTCAGC(-5/-2) BciVI 548 GGATAC GTATCC(6/5) BclI 791 TGATCA T^GATCA BfaI 553 CTAG C^TAG BglI 215 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 463 TACGTG [CT]AC^GT[GA] BsaBI 681 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1111 GAGACC GGTCTC(1/5) BsmFI 700 GTCCC GGGAC(10/14) Bsp1407I 584 TGTACA T^GTACA BspMI 287 ACCTGC ACCTGC(4/8) BsrDI 224 GCAATG GCAATG(2/0) BsrGI 584 TGTACA T^GTACA BstAPI 615 GCAAGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 166 TTTAAA TTT^AAA DraII 1025 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 1025 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 506 CCCGC CCCGC(4/6) FbaI 791 TGATCA T^GATCA HaeII 1085 GGCGCT [GA]GCGC^[CT] HinfI 133 GAGTC G^A[ACGT]TC MaeI 553 CTAG C^TAG MamI 681 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 167 TTAA T^TAA PleI 133 GAGTC GAGTC(4/5) PpuMI 1025 AGGACCT [GA]G^G[AT]CC[CT] PvuII 826 CAGCTG CAG^CTG SapI 1017 GCTCTTC GCTCTTC(1/4) SmlI 265 CTCAAG C^T[CT][GA]AG SphI 428 GCATGC GCATG^C SspBI 584 TGTACA T^GTACA StuI 653 AGGCCT AGG^CCT TaqI 131 TCGA T^CGA Tru9I 167 TTAA T^TAA TthHB8I 131 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 351 CGCG CG^CG 508 CGCG AcyI 925 GACGTC G[GA]^CG[CT]C 988 GGCGTC BanII 577 GAGCTC G[GA]GC[CT]^C 895 GAGCTC BpmI 595 CTCCAG CTGGAG(16/14) 751 CTCCAG BsaHI 925 GACGTC G[GA]^CG[CT]C 988 GGCGTC BseRI 1089 CTCCTC GAGGAG(10/8) 600 GAGGAG BsrI 219 ACTGG ACTGG(1/-1) 881 CCAGT BstUI 351 CGCG CG^CG 508 CGCG BstXI 665 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1094 CCATGAGTGTGG EcoNI 747 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1038 CCTCAGCCAGG HapII 1056 CCGG C^CGG 1083 CCGG Hin1I 925 GACGTC G[GA]^CG[CT]C 988 GGCGTC HincII 240 GTTGAC GT[CT]^[GA]AC 961 GTCAAC HindII 240 GTTGAC GT[CT]^[GA]AC 961 GTCAAC HpaII 1056 CCGG C^CGG 1083 CCGG MspA1I 826 CAGCTG C[AC]G^C[GT]G 951 CCGCTG MspI 1056 CCGG C^CGG 1083 CCGG MvnI 351 CGCG CG^CG 508 CGCG NlaIV 176 GGTTCC GG[ACGT]^[ACGT]CC 699 GGTCCC NspI 428 GCATGC [GA]CATG^[CT] 645 GCATGT PflMI 356 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 855 CCAGACGGTGG SacI 577 GAGCTC GAGCT^C 895 GAGCTC SfcI 345 CTACAG C^T[GA][CT]AG 933 CTACAG Van91I 356 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 855 CCAGACGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BalI BbsI BbvI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HgaI HhaI HinP1I HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI