(Circular) (Six-base) MAPSORT of: pFN21AE2155.seq Check: 8724 from: 1 to: 5839 pFN21AE2155 5839 bp With 182 enzymes: SgfI * August 25, 2016 14:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2934 3649 278 Size: 53 83 186 936 1398 715 2468 Fragments arranged by size: 2468 1398 936 715 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2410 3902 5286 5554 1632 Size: 778 1492 1384 268 1917 Fragments arranged by size: 1917 1492 1384 778 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 4738 5715 1203 Size: 3535 977 1327 Fragments arranged by size: 3535 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 2117 1166 Size: 951 4888 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 3582 5154 3582 Size: 1572 4267 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 3148 3374 3526 5635 1493 Size: 1655 226 152 2109 1697 Fragments arranged by size: 2109 1697 1655 226 152 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 3164 1870 Size: 73 1221 4545 Fragments arranged by size: 4545 1221 73 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4615 618 Size: 325 217 627 1373 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 2586 2904 3158 1391 Size: 1195 318 254 4072 Fragments arranged by size: 4072 1195 318 254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2365 2809 2818 3063 928 Size: 971 466 444 9 245 3704 Fragments arranged by size: 3704 971 466 444 245 9 BbvCI CC'TCA_GC Cuts at: 3044 3044 Size: 5839 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2547 3731 4941 2547 Size: 1184 1210 3445 Fragments arranged by size: 3445 1210 1184 BclI T'GATC_A Cuts at: 1548 2796 1548 Size: 1248 4591 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2226 4450 136 Size: 107 122 71 631 605 554 2224 1525 Fragments arranged by size: 2224 1525 631 605 554 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 2512 3900 5056 1790 Size: 722 1388 1156 2573 Fragments arranged by size: 2573 1388 1156 722 BmgBI CAC'GTC Cuts at: 1318 2161 1318 Size: 843 4996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2234 3131 3570 Size: 416 53 83 399 24 817 897 439 Cuts at: 3570 4528 442 Size: 958 1753 Fragments arranged by size: 1753 958 897 817 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 2741 4499 1239 Size: 1346 156 1758 2579 Fragments arranged by size: 2579 1758 1346 156 Bpu10I CC'TnA_GC Cuts at: 3044 3044 Size: 5839 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2255 3964 4829 5127 2255 Size: 1709 865 298 2967 Fragments arranged by size: 2967 1709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3110 4502 882 Size: 617 1611 1392 2219 Fragments arranged by size: 2219 1611 1392 617 BsaAI yAC'GTr Cuts at: 493 1204 2470 3630 493 Size: 711 1266 1160 2702 Fragments arranged by size: 2702 1266 1160 711 BsaBI GATnn'nnATC Cuts at: 2690 5833 2690 Size: 3143 2696 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2931 2994 3646 4028 Size: 53 83 186 936 1398 63 652 382 Cuts at: 4028 4663 275 Size: 635 1451 Fragments arranged by size: 1451 1398 936 652 635 382 186 83 63 53 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2599 2629 1178 Size: 30 165 30 1196 30 4388 Fragments arranged by size: 4388 1196 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2620 3085 1186 Size: 1434 465 3940 Fragments arranged by size: 3940 1434 465 BseYI C'CCAG_C Cuts at: 5042 5538 5042 Size: 496 5343 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2150 2571 2586 2904 3900 3985 5056 2150 Size: 421 15 318 996 85 1071 2933 Fragments arranged by size: 2933 1071 996 421 318 85 15 BsmI GAATG_Cn' Cuts at: 3350 3443 3350 Size: 93 5746 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2512 2571 2586 2904 3158 3900 Size: 399 360 362 59 15 318 254 742 Cuts at: 3900 3985 5056 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 362 360 318 254 85 59 15 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2301 3194 844 Size: 1457 893 3489 Fragments arranged by size: 3489 1457 893 BsrBI CCG'CTC Cuts at: 1394 1823 3726 1394 Size: 429 1903 3507 Fragments arranged by size: 3507 1903 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2236 4334 4508 65 Size: 1183 988 2098 174 1396 Fragments arranged by size: 2098 1396 1183 988 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 2589 96 Size: 2493 3346 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 2626 794 Size: 1832 4007 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5839 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2677 3106 2011 Size: 666 429 4744 Fragments arranged by size: 4744 666 429 BstYI r'GATC_y Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834 Size: 1197 367 385 17 1452 86 12 346 Cuts at: 5834 1972 Size: 1977 Fragments arranged by size: 1977 1452 1197 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4348 Fragments arranged by size: 4348 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2639 2644 2785 2905 487 Size: 683 56 1413 5 141 120 3421 Fragments arranged by size: 3421 1413 683 141 120 56 5 BtsI GCAGTG_nn' Cuts at: 2620 3071 3355 4149 4169 2620 Size: 451 284 794 20 4290 Fragments arranged by size: 4290 794 451 284 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 2173 3143 2173 Size: 970 4869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 2472 2715 Size: 54 1425 50 275 18 528 114 243 Cuts at: 2715 2868 3286 4175 5804 8 Size: 153 418 889 1629 43 Fragments arranged by size: 1629 1425 889 528 418 275 243 153 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2327 3020 3029 3769 1353 Size: 974 693 9 740 3423 Fragments arranged by size: 3423 974 740 693 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2584 2902 2584 Size: 318 5521 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 2585 2741 3902 4499 5286 5554 Size: 393 778 175 156 1161 597 787 268 Cuts at: 5554 1239 Size: 1524 Fragments arranged by size: 1524 1161 787 778 597 393 268 175 156 EcoNI CCTnn'n_nnAGG Cuts at: 2756 3047 2756 Size: 291 5548 EcoO109I rG'GnC_Cy Cuts at: 3031 3031 Size: 5839 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2437 2416 Size: 21 5818 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 3094 4986 1678 Size: 1416 1892 2531 Fragments arranged by size: 2531 1892 1416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2622 2654 4480 4512 4554 Size: 32 135 32 1050 32 1826 32 42 Cuts at: 4554 4586 1373 Size: 32 2626 Fragments arranged by size: 2626 1826 1050 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2247 2968 3183 3429 669 Size: 988 281 309 721 215 246 3079 Fragments arranged by size: 3079 988 721 309 281 246 215 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4953 5137 1305 Size: 3648 184 2007 Fragments arranged by size: 3648 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2474 2717 2870 10 Size: 54 1425 343 642 243 153 2979 Fragments arranged by size: 2979 1425 642 343 243 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 2675 2792 2804 3104 3797 4156 Size: 648 951 558 117 12 300 693 359 Cuts at: 4156 4315 518 Size: 159 2042 Fragments arranged by size: 2042 951 693 648 558 359 300 159 117 12 MspA1I CmG'CkG Cuts at: 1606 2833 2958 3255 3932 5080 5325 1606 Size: 1227 125 297 677 1148 245 2120 Fragments arranged by size: 2120 1227 1148 677 297 245 125 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4348 Fragments arranged by size: 4348 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 2437 2654 3197 4742 1132 Size: 97 1208 217 543 1545 2229 Fragments arranged by size: 2229 1545 1208 543 217 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2367 2866 1108 Size: 1259 499 4081 Fragments arranged by size: 4081 1259 499 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3031 3031 Size: 5839 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PstI C_TGCA'G Cuts at: 830 3191 830 Size: 2361 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2833 2833 Size: 5839 SacI G_AGCT'C Cuts at: 2586 2904 2586 Size: 318 5521 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SapI GCTCTTCn'nnn_ Cuts at: 3029 3029 Size: 5839 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SfcI C'TryA_G Cuts at: 826 1045 2350 2938 3187 4322 5003 5194 826 Size: 219 1305 588 249 1135 681 191 1471 Fragments arranged by size: 1471 1305 1135 681 588 249 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 3166 1872 Size: 1294 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2270 3943 4844 5106 820 Size: 197 926 327 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 327 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 2437 3197 1132 Size: 1305 760 3774 Fragments arranged by size: 3774 1305 760 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 4696 StyI C'CwwG_G Cuts at: 513 1065 2004 3278 513 Size: 552 939 1274 3074 Fragments arranged by size: 3074 1274 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 3866 3883 4036 4221 4666 325 Size: 1255 717 1569 17 153 185 445 1498 Fragments arranged by size: 1569 1498 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 2589 4085 Size: 274 80 33 51 1443 219 393 1496 Cuts at: 4085 5797 96 Size: 1712 138 Fragments arranged by size: 1712 1496 1443 393 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2599 2631 5799 5831 2599 Size: 32 3168 32 2607 Fragments arranged by size: 3168 2607 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5839 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 3164 1870 Size: 1294 4545 XmnI GAAnn'nnTTC Cuts at: 3968 3968 Size: 5839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2932 3647 276 Size: 53 83 186 936 1398 715 2468 Fragments arranged by size: 2468 1398 936 715 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI