Restriction map for
pFN21AE6111
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaII AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AhdI 274 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 19 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 308 GGGCCC GGGCC^C
AspEI 274 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 298 GAGCAC G[AT]GC[AT]^C
BanI 319 GGCGCC G^G[CT][GA]CC
BbeI 319 GGCGCC GGCGC^C
BcgI 113 GCATGGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 211 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 415 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 415 GCTGAGC GC^T[ACGT]AGC
BsgI 58 CTGCAC GTGCAG(16/14)
BsiHKAI 298 GAGCAC G[AT]GC[AT]^C
BspHI 197 TCATGA T^CATGA
BstEII 150 GGTGACC G^GT[ACGT]ACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
BstXI 77 CCAGCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 415 GCTGAGC GC^T[ACGT]AGC
DpnII 201 GATC ^GATC
DraII 308 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 347 CCGCGG C^C[GA][CT]GG
EaeI 218 TGGCCG [CT]^GGCC[GA]
Eam1105I 274 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 402 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 308 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
HphI 150 GGTGA GGTGA(8/7)
KasI 319 GGCGCC G^GCGCC
KspI 347 CCGCGG CCGC^GG
MaeIII 151 GTGAC ^GT[ACGT]AC
MboI 201 GATC ^GATC
MboII 203 TCTTC GAAGA(8/7)
MslI 108 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 319 GGCGCC GG^CGCC
NdeII 201 GATC ^GATC
PaeR7I 52 CTCGAG C^TCGAG
PstI 6 CTGCAG CTGCA^G
RcaI 197 TCATGA T^CATGA
SacII 347 CCGCGG CCGC^GG
Sau3AI 201 GATC ^GATC
SfcI 6 CTGCAG C^T[GA][CT]AG
SfoI 319 GGCGCC GGC^GCC
SmaI 312 CCCGGG CCC^GGG
SmlI 52 CTCGAG C^T[CT][GA]AG
StuI 225 AGGCCT AGG^CCT
Tsp45I 151 GTGAC ^GT[GC]AC
XcmI 104 CCAGCATGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 52 CTCGAG C^TCGAG
XmaCI 312 CCCGGG C^CCGGG
XmaI 312 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 145 CGCG CG^CG
348 CGCG
AvaI 52 CTCGAG C^[CT]CG[GA]G
312 CCCGGG
BanII 308 GGGCCC G[GA]GC[CT]^C
351 GGGCTC
BsmFI 101 GTCCC GGGAC(10/14)
282 GTCCC
BsoBI 52 CTCGAG C^[CT]CG[GA]G
312 CCCGGG
BsrBI 246 CCGCTC CCGCTC(-3/-3)
418 GAGCGG
BstF5I 186 CATCC GGATG(2/0)
231 CATCC
BstUI 145 CGCG CG^CG
348 CGCG
DdeI 37 CTGAG C^T[ACGT]AG
416 CTGAG
EcoT14I 161 CCAAGG C^C[AT][AT]GG
269 CCAAGG
FokI 186 CATCC GGATG(9/13)
231 CATCC
HgaI 172 GACGC GACGC(5/10)
334 GACGC
MvnI 145 CGCG CG^CG
348 CGCG
StyI 161 CCAAGG C^C[AT][AT]GG
269 CCAAGG
TaqI 53 TCGA T^CGA
263 TCGA
TthHB8I 53 TCGA T^CGA
263 TCGA
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcyI AluI BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI TseI