################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HinfI HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 1012 GACGTC GACGT^C AccI 766 GTCTAC GT^[AC][GT]AC Asp700I 444 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 798 GGCGCC GGCGC^C BbsI 448 GTCTTC GAAGAC(2/6) BfaI 892 CTAG C^TAG BglI 714 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 560 AGATCT A^GATCT BpuAI 448 GTCTTC GAAGAC(2/6) BsaI 142 GAGACC GGTCTC(1/5) BsaWI 665 TCCGGT [AT]^CCGG[AT] BsgI 999 CTGCAC GTGCAG(16/14) BsmAI 142 GAGAC GTCTC(1/5) Bsp1407I 746 TGTACA T^GTACA BsrGI 746 TGTACA T^GTACA EaeI 263 CGGCCA [CT]^GGCC[GA] EarI 34 CTCTTC CTCTTC(1/4) EcoT14I 960 CCAAGG C^C[AT][AT]GG HincII 931 GTCAAC GT[CT]^[GA]AC HindII 931 GTCAAC GT[CT]^[GA]AC KasI 798 GGCGCC G^GCGCC Ksp632I 34 CTCTTC CTCTTC(1/4) MaeI 892 CTAG C^TAG MaeII 1013 ACGT A^CGT NarI 798 GGCGCC GG^CGCC PflMI 636 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 569 GGGTCCC [GA]G^G[AT]CC[CT] SacI 538 GAGCTC GAGCT^C SapI 33 GCTCTTC GCTCTTC(1/4) SfaNI 507 GATGC GCATC(5/9) SfoI 798 GGCGCC GGC^GCC SspBI 746 TGTACA T^GTACA StyI 960 CCAAGG C^C[AT][AT]GG Tsp509I 871 AATT ^AATT Van91I 636 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 444 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 220 CGCG CG^CG 1089 CGCG AlwNI 993 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1036 CAGGTGCTG AspHI 538 GAGCTC G[AT]GC[AT]^C 754 GAGCAC BanII 31 GGGCTC G[GA]GC[CT]^C 538 GAGCTC BbvCI 728 CCTCAGC CCTCAGC(-5/-2) 459 GCTGAGG BsiHKAI 538 GAGCTC G[AT]GC[AT]^C 754 GAGCAC BspMI 296 ACCTGC ACCTGC(4/8) 1035 GCAGGT BstAPI 992 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1035 GCAGGTGCTGC BstUI 220 CGCG CG^CG 1089 CGCG DraII 569 GGGTCCC [GA]G^G[ACGT]CC[CT] 659 GGGGCCT EcoNI 42 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 702 CCTTCGGAAGG EcoO109I 569 GGGTCCC [GA]G^G[ACGT]CC[CT] 659 GGGGCCT FauI 218 CCCGC CCCGC(4/6) 314 CCCGC HaeII 798 GGCGCC [GA]GCGC^[CT] 1072 AGCGCC HgaI 1087 GACGC GACGC(5/10) 368 GCGTC HphI 350 TCACC GGTGA(8/7) 422 TCACC MaeIII 275 GTGAC ^GT[ACGT]AC 669 GTCAC MspA1I 742 CAGCTG C[AC]G^C[GT]G 910 CAGCTG MvnI 220 CGCG CG^CG 1089 CGCG PstI 913 CTGCAG CTGCA^G 1033 CTGCAG PvuII 742 CAGCTG CAG^CTG 910 CAGCTG SfcI 913 CTGCAG C^T[GA][CT]AG 1033 CTGCAG Tsp45I 275 GTGAC ^GT[GC]AC 669 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BanI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SmlI TaqI TseI TspRI TthHB8I XcmI XhoII