(Circular) (Six-base) MAPSORT of: pFN21AE1465.seq Check: 7395 from: 1 to: 5833 pFN21AE1465 5833 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3031 3031 Size: 5833 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3021 3643 278 Size: 53 83 186 936 1485 622 2468 Fragments arranged by size: 2468 1485 936 622 186 83 53 AccI GT'mk_AC Cuts at: 1937 2772 3176 1937 Size: 835 404 4594 Fragments arranged by size: 4594 835 404 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3896 5280 5548 1632 Size: 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 3011 1166 Size: 1845 3988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3098 1184 Size: 1914 3919 AlwNI CAG_nnn'CTG Cuts at: 3003 3046 3576 5148 3003 Size: 43 530 1572 3688 Fragments arranged by size: 3688 1572 530 43 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 3142 3368 3520 5629 1493 Size: 1649 226 152 2109 1697 Fragments arranged by size: 2109 1697 1649 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2469 2716 2803 3154 4609 Size: 325 217 627 682 247 87 351 1455 Cuts at: 4609 618 Size: 1842 Fragments arranged by size: 1842 1455 682 627 351 325 247 217 87 BanII G_rGCy'C Cuts at: 1391 2040 2547 3152 1391 Size: 649 507 605 4072 Fragments arranged by size: 4072 649 605 507 BbeI G_GCGC'C Cuts at: 2807 2807 Size: 5833 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2446 928 Size: 971 547 4315 Fragments arranged by size: 4315 971 547 BbvCI CC'TCA_GC Cuts at: 2465 2734 2465 Size: 269 5564 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4024 4058 1903 Size: 34 50 34 2003 34 3678 Fragments arranged by size: 3678 2003 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2725 4444 136 Size: 107 122 71 631 605 1053 1719 1525 Fragments arranged by size: 1719 1525 1053 631 605 122 107 71 BglII A'GATC_T Cuts at: 2565 5828 2565 Size: 3263 2570 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 3894 5050 1790 Size: 2104 1156 2573 Fragments arranged by size: 2573 2104 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 2927 3564 Size: 416 53 83 399 24 636 874 637 Cuts at: 3564 4522 442 Size: 958 1753 Fragments arranged by size: 1753 958 874 637 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 2495 2612 2621 2969 4493 1239 Size: 960 296 117 9 348 1524 2579 Fragments arranged by size: 2579 1524 960 348 296 117 9 Bpu10I CC'TnA_GC Cuts at: 2465 2734 2465 Size: 269 5564 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 2762 2912 3958 4823 5121 2279 Size: 483 150 1046 865 298 2991 Fragments arranged by size: 2991 1046 865 483 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2141 4496 882 Size: 617 642 2355 2219 Fragments arranged by size: 2355 2219 642 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2373 2804 3018 3640 Size: 53 83 186 936 840 431 214 622 Cuts at: 3640 4022 4657 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 840 635 622 431 382 214 186 83 53 BsaWI w'CCGG_w Cuts at: 2670 4266 4664 4938 5085 5496 2670 Size: 1596 398 274 147 411 3007 Fragments arranged by size: 3007 1596 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2468 2498 1178 Size: 30 165 30 1065 30 4513 Fragments arranged by size: 4513 1065 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5833 BseYI C'CCAG_C Cuts at: 5036 5532 5036 Size: 496 5337 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2989 1163 Size: 573 1253 4007 Fragments arranged by size: 4007 1253 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2547 2763 3894 3979 5050 2547 Size: 216 1131 85 1071 3330 Fragments arranged by size: 3330 1131 1071 216 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2547 2763 3152 3894 3979 5050 Size: 399 250 507 216 389 742 85 1071 Cuts at: 5050 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 507 399 389 250 216 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 3031 3188 844 Size: 1466 721 157 3489 Fragments arranged by size: 3489 1466 721 157 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 2751 96 Size: 2655 3178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 3003 3046 794 Size: 2209 43 3581 Fragments arranged by size: 3581 2209 43 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2218 2565 2637 2925 3163 3530 3915 3932 Size: 246 347 72 288 238 367 385 17 Cuts at: 3932 5384 5470 5482 5828 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 347 346 288 246 238 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2265 2413 3009 513 Size: 552 1200 148 596 3337 Fragments arranged by size: 3337 1200 596 552 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 2015 3349 4143 4163 2015 Size: 1334 794 20 3685 Fragments arranged by size: 3685 1334 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 3280 4169 Size: 54 1425 50 275 18 438 1012 889 Cuts at: 4169 5798 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1012 889 438 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3763 1353 Size: 692 1718 3423 Fragments arranged by size: 3423 1718 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2545 2545 Size: 5833 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2495 2612 2621 2969 3896 4493 Size: 393 567 296 117 9 348 927 597 Cuts at: 4493 5280 5548 1239 Size: 787 268 1524 Fragments arranged by size: 1524 927 787 597 567 393 348 296 268 117 9 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2711 2051 Size: 660 5173 EcoO109I rG'GnC_Cy Cuts at: 2575 2665 2575 Size: 90 5743 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 2807 3081 4980 1678 Size: 1129 274 1899 2531 Fragments arranged by size: 2531 1899 1129 274 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2503 2535 4474 4506 4548 Size: 32 135 32 931 32 1939 32 42 Cuts at: 4548 4580 1373 Size: 32 2626 Fragments arranged by size: 2626 1939 931 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2938 3177 3423 669 Size: 988 281 1000 239 246 3079 Fragments arranged by size: 3079 1000 988 281 246 239 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KasI G'GCGC_C Cuts at: 2803 2803 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2650 2889 4947 5131 1305 Size: 1345 239 2058 184 2007 Fragments arranged by size: 2058 2007 1345 239 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2766 3011 3791 4150 4309 518 Size: 648 1104 496 245 780 359 159 2042 Fragments arranged by size: 2042 1104 780 648 496 359 245 159 MspA1I CmG'CkG Cuts at: 1606 2749 2917 3249 3926 5074 5319 1606 Size: 1143 168 332 677 1148 245 2120 Fragments arranged by size: 2120 1148 1143 677 332 245 168 NarI GG'CG_CC Cuts at: 2804 2804 Size: 5833 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5281 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2647 1108 Size: 902 637 4294 Fragments arranged by size: 4294 902 637 PfoI T'CCnGG_A Cuts at: 2364 2640 2364 Size: 276 5557 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2575 2575 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 2700 3403 2700 Size: 703 5130 PstI C_TGCA'G Cuts at: 830 2922 3042 3185 830 Size: 2092 120 143 3478 Fragments arranged by size: 3478 2092 143 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2749 2917 2749 Size: 168 5665 SacI G_AGCT'C Cuts at: 2547 2547 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SanDI GG'GwC_CC Cuts at: 2575 2575 Size: 5833 SapI GCTCTTCn'nnn_ Cuts at: 2045 2045 Size: 5833 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 2918 3038 3181 4316 4997 5188 826 Size: 219 1873 120 143 1135 681 191 1471 Fragments arranged by size: 1873 1471 1135 681 219 191 143 120 SfoI GGC'GCC Cuts at: 2805 2805 Size: 5833 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2294 2741 2891 3937 4838 5100 Size: 197 926 351 447 150 1046 901 262 Cuts at: 5100 820 Size: 1553 Fragments arranged by size: 1553 1046 926 901 447 351 262 197 150 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2965 3272 513 Size: 552 1900 307 3074 Fragments arranged by size: 3074 1900 552 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2269 3860 3877 4030 4215 4660 325 Size: 1255 689 1591 17 153 185 445 1498 Fragments arranged by size: 1591 1498 1255 689 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 2334 2751 Size: 274 80 33 51 1443 297 60 417 Cuts at: 2751 4079 5791 96 Size: 1328 1712 138 Fragments arranged by size: 1712 1443 1328 417 297 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2244 2276 5793 5825 2244 Size: 32 3517 32 2252 Fragments arranged by size: 3517 2252 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2437 2587 2854 2437 Size: 150 267 5416 Fragments arranged by size: 5416 267 150 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 2453 3962 2453 Size: 1509 4324 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3019 3641 276 Size: 53 83 186 936 1485 622 2468 Fragments arranged by size: 2468 1485 936 622 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI