(Linear) (Six-base) MAPSORT of: KIEE1465.seq Check: 1470 from: 1 to: 1128 KIEE1465.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1026 1128 Size: 1026 102 AatII G_ACGT'C Cuts at: 0 1016 1128 Size: 1016 112 AccI GT'mk_AC Cuts at: 0 767 1128 Size: 767 361 AleI CACnn'nnGTG Cuts at: 0 1006 1128 Size: 1006 122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1093 1128 Size: 1093 35 AlwNI CAG_nnn'CTG Cuts at: 0 998 1041 1128 Size: 998 43 87 Fragments arranged by size: 998 87 43 BanI G'GyrC_C Cuts at: 0 464 711 798 1128 Size: 464 247 87 330 Fragments arranged by size: 464 330 247 87 BanII G_rGCy'C Cuts at: 0 35 542 1128 Size: 35 507 586 Fragments arranged by size: 586 507 35 BbeI G_GCGC'C Cuts at: 0 802 1128 Size: 802 326 BbsI GAAGACnn'nnnn_ Cuts at: 0 441 1128 Size: 441 687 BbvCI CC'TCA_GC Cuts at: 0 460 729 1128 Size: 460 269 399 Fragments arranged by size: 460 399 269 BglI GCCn_nnn'nGGC Cuts at: 0 720 1128 Size: 720 408 BglII A'GATC_T Cuts at: 0 560 1128 Size: 560 568 BmrI ACTGGGnnnn_n' Cuts at: 0 48 922 1128 Size: 48 874 206 Fragments arranged by size: 874 206 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 194 490 607 616 964 1128 Size: 194 296 117 9 348 164 Fragments arranged by size: 348 296 194 164 117 9 Bpu10I CC'TnA_GC Cuts at: 0 460 729 1128 Size: 460 269 399 Fragments arranged by size: 460 399 269 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 757 907 1128 Size: 274 483 150 221 Fragments arranged by size: 483 274 221 150 BsaI GGTCTCn'nnnn_ Cuts at: 0 136 1128 Size: 136 992 BsaHI Gr'CG_yC Cuts at: 0 368 799 1013 1128 Size: 368 431 214 115 Fragments arranged by size: 431 368 214 115 BsaWI w'CCGG_w Cuts at: 0 665 1128 Size: 665 463 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 463 493 1128 Size: 463 30 635 Fragments arranged by size: 635 463 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 984 1128 Size: 984 144 BsiHKAI G_wGCw'C Cuts at: 0 542 758 1128 Size: 542 216 370 Fragments arranged by size: 542 370 216 Bsp1286I G_dGCh'C Cuts at: 0 35 542 758 1128 Size: 35 507 216 370 Fragments arranged by size: 507 370 216 35 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 305 1026 1128 Size: 305 721 102 Fragments arranged by size: 721 305 102 BsrGI T'GTAC_A Cuts at: 0 746 1128 Size: 746 382 BstAPI GCAn_nnn'nTGC Cuts at: 0 998 1041 1128 Size: 998 43 87 Fragments arranged by size: 998 87 43 BstYI r'GATC_y Cuts at: 0 213 560 632 920 1128 Size: 213 347 72 288 208 Fragments arranged by size: 347 288 213 208 72 BtgI C'CryG_G Cuts at: 0 260 408 1004 1128 Size: 260 148 596 124 Fragments arranged by size: 596 260 148 124 BtsI GCAGTG_nn' Cuts at: 0 10 1128 Size: 10 1118 EaeI y'GGCC_r Cuts at: 0 263 1128 Size: 263 865 EarI CTCTTCn'nnn_ Cuts at: 0 40 1128 Size: 40 1088 EcoICRI GAG'CTC Cuts at: 0 540 1128 Size: 540 588 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 194 490 607 616 964 1128 Size: 194 296 117 9 348 164 Fragments arranged by size: 348 296 194 164 117 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 46 706 1128 Size: 46 660 422 Fragments arranged by size: 660 422 46 EcoO109I rG'GnC_Cy Cuts at: 0 570 660 1128 Size: 570 90 468 Fragments arranged by size: 570 468 90 HaeII r_GCGC'y Cuts at: 0 802 1076 1128 Size: 802 274 52 Fragments arranged by size: 802 274 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 498 530 1128 Size: 498 32 598 Fragments arranged by size: 598 498 32 HincII GTy'rAC Cuts at: 0 933 1128 Size: 933 195 KasI G'GCGC_C Cuts at: 0 798 1128 Size: 798 330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 645 884 1128 Size: 645 239 244 Fragments arranged by size: 645 244 239 MslI CAynn'nnrTG Cuts at: 0 265 761 1006 1128 Size: 265 496 245 122 Fragments arranged by size: 496 265 245 122 MspA1I CmG'CkG Cuts at: 0 744 912 1128 Size: 744 168 216 Fragments arranged by size: 744 216 168 NarI GG'CG_CC Cuts at: 0 799 1128 Size: 799 329 PflMI CCAn_nnn'nTGG Cuts at: 0 642 1128 Size: 642 486 PfoI T'CCnGG_A Cuts at: 0 359 635 1128 Size: 359 276 493 Fragments arranged by size: 493 359 276 PpuMI rG'GwC_Cy Cuts at: 0 570 1128 Size: 570 558 PsiI TTA'TAA Cuts at: 0 695 1128 Size: 695 433 PstI C_TGCA'G Cuts at: 0 917 1037 1128 Size: 917 120 91 Fragments arranged by size: 917 120 91 PvuII CAG'CTG Cuts at: 0 744 912 1128 Size: 744 168 216 Fragments arranged by size: 744 216 168 SacI G_AGCT'C Cuts at: 0 542 1128 Size: 542 586 SanDI GG'GwC_CC Cuts at: 0 570 1128 Size: 570 558 SapI GCTCTTCn'nnn_ Cuts at: 0 40 1128 Size: 40 1088 SfcI C'TryA_G Cuts at: 0 913 1033 1128 Size: 913 120 95 Fragments arranged by size: 913 120 95 SfoI GGC'GCC Cuts at: 0 800 1128 Size: 800 328 SmlI C'TyrA_G Cuts at: 0 289 736 886 1128 Size: 289 447 150 242 Fragments arranged by size: 447 289 242 150 StyI C'CwwG_G Cuts at: 0 960 1128 Size: 960 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 264 1128 Size: 264 864 TatI w'GTAC_w Cuts at: 0 269 329 746 1128 Size: 269 60 417 382 Fragments arranged by size: 417 382 269 60 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 239 271 1128 Size: 239 32 857 Fragments arranged by size: 857 239 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 432 582 849 1128 Size: 432 150 267 279 Fragments arranged by size: 432 279 267 150 XmnI GAAnn'nnTTC Cuts at: 0 448 1128 Size: 448 680 ZraI GAC'GTC Cuts at: 0 1014 1128 Size: 1014 114 Enzymes that do cut: AarI AatII AccI AleI AlfI AlwNI BanI BanII BbeI BbsI BbvCI BglI BglII BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspMI BsrGI BstAPI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MslI MspA1I NarI PflMI PfoI PpuMI PsiI PstI PvuII SacI SanDI SapI SfcI SfoI SmlI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI