################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BbeI BbsI BclI BfaI BfrI BlnI BpuAI BsaBI BsaHI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV FbaI FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 796 CGCG CG^CG ApaI 1439 GGGCCC GGGCC^C Asp700I 774 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 925 CTCGAG C^[CT]CG[GA]G AviII 563 TGCGCA TGC^GCA BamHI 201 GGATCC G^GATCC BbrPI 427 CACGTG CAC^GTG BbvCI 196 GCTGAGG CCTCAGC(-5/-2) BciVI 6 GGATAC GTATCC(6/5) BglI 820 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 845 AGATCT A^GATCT BlpI 990 GCTGAGC GC^T[ACGT]AGC Bpu1102I 990 GCTGAGC GC^T[ACGT]AGC BsaI 959 GAGACC GGTCTC(1/5) BsiEI 619 CGGCCG CG[GA][CT]^CG BsmBI 1108 CGTCTC CGTCTC(1/5) BsmI 248 GCATTC GAATGC(1/-1) BsoBI 925 CTCGAG C^[CT]CG[GA]G BsrFI 594 GCCGGT [GA]^CCGG[CT] BssSI 971 CACGAG CACGAG(-5/-1) BstAPI 1042 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 899 TTCGAA TT^CGAA BstUI 796 CGCG CG^CG CelII 990 GCTGAGC GC^T[ACGT]AGC Cfr10I 594 GCCGGT [GA]^CCGG[CT] DraIII 1043 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 913 GACCACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 619 CGGCCG C^GGCCG EclXI 619 CGGCCG C^GGCCG Eco52I 619 CGGCCG C^GGCCG EcoNI 1227 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 406 ATGCAT ATGCA^T FspI 563 TGCGCA TGC^GCA HaeII 976 GGCGCT [GA]GCGC^[CT] MslI 1186 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 796 CGCG CG^CG NcoI 438 CCATGG C^CATGG NsiI 406 ATGCAT ATGCA^T NspV 899 TTCGAA TT^CGAA PaeR7I 925 CTCGAG C^TCGAG PflMI 1053 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 637 GACTC GAGTC(4/5) PmaCI 427 CACGTG CAC^GTG PmlI 427 CACGTG CAC^GTG PpuMI 320 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 447 CAGCTG CAG^CTG SacI 241 GAGCTC GAGCT^C SfcI 103 CTACAG C^T[GA][CT]AG SfuI 899 TTCGAA TT^CGAA SgrAI 593 CGCCGGTG C[GA]^CCGG[CT]G Van91I 1053 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 925 CTCGAG C^TCGAG XmnI 774 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 762 GAATTT [GA]^AATT[CT] 1348 GAATTT AfaI 738 GTAC GT^AC 1389 GTAC Alw44I 968 GTGCAC G^TGCAC 1040 GTGCAC ApaLI 968 GTGCAC G^TGCAC 1040 GTGCAC ApoI 762 GAATTT [GA]^AATT[CT] 1348 GAATTT BsaAI 427 CACGTG [CT]AC^GT[GA] 559 TACGTG BsgI 1183 CTGCAC GTGCAG(16/14) 598 GTGCAG BspMI 485 ACCTGC ACCTGC(4/8) 536 ACCTGC BsrBI 544 CCGCTC CCGCTC(-3/-3) 469 GAGCGG BsrDI 192 CATTGC GCAATG(2/0) 1196 GCAATG BstYI 201 GGATCC [GA]^GATC[CT] 845 AGATCT DsaI 438 CCATGG C^C[GA][CT]GG 963 CCGTGG EarI 778 CTCTTC CTCTTC(1/4) 784 CTCTTC EcoT14I 438 CCATGG C^C[AT][AT]GG 809 CCTTGG FauI 1430 CCCGC CCCGC(4/6) 1437 GCGGG HincII 673 GTTGAC GT[CT]^[GA]AC 1464 GTCAAC HindII 673 GTTGAC GT[CT]^[GA]AC 1464 GTCAAC Ksp632I 778 CTCTTC CTCTTC(1/4) 784 CTCTTC MflI 201 GGATCC [GA]^GATC[CT] 845 AGATCT RsaI 738 GTAC GT^AC 1389 GTAC SexAI 731 ACCTGGT A^CC[AT]GGT 1031 ACCAGGT SmlI 841 CTCAAG C^T[CT][GA]AG 925 CTCGAG SphI 404 GCATGC GCATG^C 829 GCATGC StyI 438 CCATGG C^C[AT][AT]GG 809 CCTTGG TfiI 774 GAATC G^A[AT]TC 1446 GAATC XhoII 201 GGATCC [GA]^GATC[CT] 845 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbvI BcgI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I