(Circular) (Six-base) MAPSORT of: pFN21AE6422.seq Check: 2571 from: 1 to: 6208 pFN21AE6422 6208 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 4271 5655 5923 1632 Size: 683 1956 1384 268 1917 Fragments arranged by size: 1956 1917 1384 683 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 3195 1166 Size: 2029 4179 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2324 2356 2324 Size: 32 6176 AlwNI CAG_nnn'CTG Cuts at: 2141 2525 3064 3319 3951 5523 2141 Size: 384 539 255 632 1572 2826 Fragments arranged by size: 2826 1572 632 539 384 255 ApaI G_GGCC'C Cuts at: 3448 3448 Size: 6208 ApaLI G'TGCA_C Cuts at: 2973 3045 4265 5421 2973 Size: 72 1220 1156 3760 Fragments arranged by size: 3760 1220 1156 72 ApoI r'AATT_y Cuts at: 1493 2767 3353 3517 3743 3895 6004 1493 Size: 1274 586 164 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 586 226 164 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2930 3533 1870 Size: 73 987 603 4545 Fragments arranged by size: 4545 987 603 73 BamHI G'GATC_C Cuts at: 2206 3538 3905 2206 Size: 1332 367 4509 Fragments arranged by size: 4509 1332 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2478 2513 3053 3529 4984 Size: 325 217 627 691 35 540 476 1455 Cuts at: 4984 618 Size: 1842 Fragments arranged by size: 1842 1455 691 627 540 476 325 217 35 BanII G_rGCy'C Cuts at: 1391 2250 2673 3219 3448 3527 1391 Size: 859 423 546 229 79 4072 Fragments arranged by size: 4072 859 546 423 229 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5237 BbvCI CC'TCA_GC Cuts at: 2202 2202 Size: 6208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2452 2486 2752 2786 3274 Size: 34 50 34 431 34 266 34 488 Cuts at: 3274 3308 4399 4433 1903 Size: 34 1091 34 3678 Fragments arranged by size: 3678 1091 488 431 266 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2005 4100 5310 2005 Size: 2095 1210 2903 Fragments arranged by size: 2903 2095 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BfrBI ATG'CAT Cuts at: 2413 2413 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 4819 136 Size: 107 122 71 631 605 1159 1988 1525 Fragments arranged by size: 1988 1525 1159 631 605 122 107 71 BglII A'GATC_T Cuts at: 2850 6203 2850 Size: 3353 2855 BlpI GC'TnA_GC Cuts at: 2996 3625 2996 Size: 629 5579 Bme1580I G_kGCm'C Cuts at: 1790 2235 2977 3049 3058 3448 4269 5425 1790 Size: 445 742 72 9 390 821 1156 2573 Fragments arranged by size: 2573 1156 821 742 445 390 72 9 BmgBI CAC'GTC Cuts at: 1318 2162 2581 1318 Size: 844 419 4945 Fragments arranged by size: 4945 844 419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2702 2918 3052 Size: 416 53 83 399 24 1285 216 134 Cuts at: 3052 3939 4897 442 Size: 887 958 1753 Fragments arranged by size: 1753 1285 958 887 416 399 216 134 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2103 2504 2744 4868 1239 Size: 864 401 240 2124 2579 Fragments arranged by size: 2579 2124 864 401 240 Bpu10I CC'TnA_GC Cuts at: 2202 2714 2202 Size: 512 5696 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 4333 5198 5496 2831 Size: 1502 865 298 3543 Fragments arranged by size: 3543 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2958 4871 882 Size: 617 1459 1913 2219 Fragments arranged by size: 2219 1913 1459 617 BsaAI yAC'GTr Cuts at: 493 1204 2434 2566 3999 493 Size: 711 1230 132 1433 2702 Fragments arranged by size: 2702 1433 1230 711 132 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4015 4397 5032 275 Size: 53 83 186 936 2482 382 635 1451 Fragments arranged by size: 2482 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2795 2825 1178 Size: 30 165 30 657 30 705 30 4561 Fragments arranged by size: 4561 705 657 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 2228 2681 2741 2948 1186 Size: 855 187 453 60 207 4446 Fragments arranged by size: 4446 855 453 207 187 60 BseYI C'CCAG_C Cuts at: 2042 2616 3455 5411 5907 2042 Size: 574 839 1956 496 2343 Fragments arranged by size: 2343 1956 839 574 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 3173 1163 Size: 573 888 549 4198 Fragments arranged by size: 4198 888 573 549 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2627 3658 4419 5447 1540 Size: 275 186 626 1031 761 1028 2301 Fragments arranged by size: 2301 1031 1028 761 626 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 2977 3021 3049 3246 4269 4354 5425 2250 Size: 727 44 28 197 1023 85 1071 3033 Fragments arranged by size: 3033 1071 1023 727 197 85 44 28 BsmI GAATG_Cn' Cuts at: 2253 3719 3812 2253 Size: 1466 93 4649 Fragments arranged by size: 4649 1466 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3119 3119 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2250 2673 2977 3021 3049 3058 Size: 399 445 15 423 304 44 28 9 Cuts at: 3058 3219 3246 3448 3527 4269 4354 5425 1391 Size: 161 27 202 79 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 445 423 399 304 202 161 85 79 44 28 27 15 9 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 2550 3563 844 Size: 1655 51 1013 3489 Fragments arranged by size: 3489 1655 1013 51 BsrBI CCG'CTC Cuts at: 1394 1823 2476 2551 4095 1394 Size: 429 653 75 1544 3507 Fragments arranged by size: 3507 1544 653 429 75 BsrDI GCAATG_nn' Cuts at: 65 1248 2196 3208 4703 4877 65 Size: 1183 948 1012 1495 174 1396 Fragments arranged by size: 1495 1396 1183 1012 948 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2599 4852 5039 1700 Size: 110 789 2253 187 2869 Fragments arranged by size: 2869 2253 789 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 2976 4268 5280 2976 Size: 1292 1012 3904 Fragments arranged by size: 3904 1292 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3053 794 Size: 2259 3949 BstBI TT'CG_AA Cuts at: 2905 5012 2905 Size: 2107 4101 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2206 2850 3538 3905 4290 4307 5759 5845 Size: 234 644 688 367 385 17 1452 86 Cuts at: 5845 5857 6203 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 688 644 385 367 346 234 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2443 2968 513 Size: 552 1378 525 3753 Fragments arranged by size: 3753 1378 552 525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2399 487 Size: 683 56 1173 4296 Fragments arranged by size: 4296 1173 683 56 BtsI GCAGTG_nn' Cuts at: 3477 3724 4518 4538 3477 Size: 247 794 20 5147 Fragments arranged by size: 5147 794 247 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DraIII CAC_nnn'GTG Cuts at: 3053 3053 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2924 5215 809 Size: 507 1608 2291 1802 Fragments arranged by size: 2291 1802 1608 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2624 3139 Size: 54 1425 50 275 18 393 401 515 Cuts at: 3139 3403 3655 4544 6173 8 Size: 264 252 889 1629 43 Fragments arranged by size: 1629 1425 889 515 401 393 275 264 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2624 3655 1537 Size: 275 812 1031 4090 Fragments arranged by size: 4090 1031 812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2789 2795 4138 1353 Size: 1436 6 1343 3423 Fragments arranged by size: 3423 1436 1343 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2586 4795 5169 5315 387 Size: 1064 138 997 2209 374 146 1280 Fragments arranged by size: 2209 1280 1064 997 374 146 138 EcoICRI GAG'CTC Cuts at: 2248 2248 Size: 6208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2315 2504 2744 4271 4868 5655 Size: 393 471 212 189 240 1527 597 787 Cuts at: 5655 5923 1239 Size: 268 1524 Fragments arranged by size: 1527 1524 787 597 471 393 268 240 212 189 EcoNI CCTnn'n_nnAGG Cuts at: 3236 3236 Size: 6208 EcoO109I rG'GnC_Cy Cuts at: 2326 3409 3445 2326 Size: 1083 36 5089 Fragments arranged by size: 5089 1083 36 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FspI TGC'GCA Cuts at: 2570 4714 2570 Size: 2144 4064 HaeII r_GCGC'y Cuts at: 1678 2985 5355 1678 Size: 1307 2370 2531 Fragments arranged by size: 2531 2370 1307 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2795 2827 4849 Size: 32 135 32 488 32 703 32 2022 Cuts at: 4849 4881 4923 4955 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2022 703 488 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2680 3471 3552 3798 669 Size: 988 281 742 791 81 246 3079 Fragments arranged by size: 3079 988 791 742 281 246 81 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 2162 2664 5322 5506 1305 Size: 711 146 502 2658 184 2007 Fragments arranged by size: 2658 2007 711 502 184 146 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 3141 3405 10 Size: 54 1425 343 393 916 264 2813 Fragments arranged by size: 2813 1425 916 393 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 3195 4166 4525 4684 518 Size: 648 2029 971 359 159 2042 Fragments arranged by size: 2042 2029 971 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2403 2454 3442 3624 4301 5449 5694 1606 Size: 797 51 988 182 677 1148 245 2120 Fragments arranged by size: 2120 1148 988 797 677 245 182 51 NcoI C'CATG_G Cuts at: 513 1065 2443 513 Size: 552 1378 4278 Fragments arranged by size: 4278 1378 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NsiI A_TGCA'T Cuts at: 2415 2415 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2413 2467 2838 3331 3566 5111 1132 Size: 97 1184 54 371 493 235 1545 2229 Fragments arranged by size: 2229 1545 1184 493 371 235 97 54 PasI CC'CwG_GG Cuts at: 1348 2166 1348 Size: 818 5390 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 3064 1108 Size: 1956 4252 PfoI T'CCnGG_A Cuts at: 2150 2307 3318 3348 2150 Size: 157 1011 30 5010 Fragments arranged by size: 5010 1011 157 30 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PmlI CAC'GTG Cuts at: 2434 2434 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2326 2326 Size: 6208 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 3444 3444 Size: 6208 PspXI vC'TCGA_Gb Cuts at: 2930 2930 Size: 6208 PstI C_TGCA'G Cuts at: 830 3560 830 Size: 2730 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2454 2454 Size: 6208 SacI G_AGCT'C Cuts at: 2250 2250 Size: 6208 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SexAI A'CCwGG_T Cuts at: 2736 3036 2736 Size: 300 5908 SfcI C'TryA_G Cuts at: 826 1045 2108 3556 4691 5372 5563 826 Size: 219 1063 1448 1135 681 191 1471 Fragments arranged by size: 1471 1448 1135 1063 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 2599 1700 Size: 899 5309 SmaI CCC'GGG Cuts at: 1872 3535 1872 Size: 1663 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 2930 4312 5213 5475 820 Size: 197 926 903 84 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 903 901 262 197 84 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 2413 2838 3566 1132 Size: 1281 425 728 3774 Fragments arranged by size: 3774 1281 728 425 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2443 2814 3647 513 Size: 552 1378 371 833 3074 Fragments arranged by size: 3074 1378 833 552 371 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 2937 4235 4252 4405 4590 5035 Size: 1255 498 859 1298 17 153 185 445 Cuts at: 5035 325 Size: 1498 Fragments arranged by size: 1498 1298 1255 859 498 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4454 6166 96 Size: 274 80 33 51 1443 2477 1712 138 Fragments arranged by size: 2477 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 6168 6200 2198 Size: 32 3938 32 2206 Fragments arranged by size: 3938 2206 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 2930 1943 Size: 987 5221 XmaI C'CCGG_G Cuts at: 1870 3533 1870 Size: 1663 4545 XmnI GAAnn'nnTTC Cuts at: 2783 4337 2783 Size: 1554 4654 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI