(Linear) (Six-base) MAPSORT of: KIEE6422.seq Check: 4369 from: 1 to: 1503 KIEE6422.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 1503 Size: 310 1193 AleI CACnn'nnGTG Cuts at: 0 1190 1503 Size: 1190 313 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 319 351 1503 Size: 319 32 1152 Fragments arranged by size: 1152 319 32 AlwNI CAG_nnn'CTG Cuts at: 0 136 520 1059 1314 1503 Size: 136 384 539 255 189 Fragments arranged by size: 539 384 255 189 136 ApaI G_GGCC'C Cuts at: 0 1443 1503 Size: 1443 60 ApaLI G'TGCA_C Cuts at: 0 968 1040 1503 Size: 968 72 463 Fragments arranged by size: 968 463 72 ApoI r'AATT_y Cuts at: 0 762 1348 1503 Size: 762 586 155 Fragments arranged by size: 762 586 155 AvaI C'yCGr_G Cuts at: 0 925 1503 Size: 925 578 BamHI G'GATC_C Cuts at: 0 201 1503 Size: 201 1302 BanI G'GyrC_C Cuts at: 0 473 508 1048 1503 Size: 473 35 540 455 Fragments arranged by size: 540 473 455 35 BanII G_rGCy'C Cuts at: 0 245 668 1214 1443 1503 Size: 245 423 546 229 60 Fragments arranged by size: 546 423 245 229 60 BbvCI CC'TCA_GC Cuts at: 0 197 1503 Size: 197 1306 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 447 481 747 781 1269 1303 1503 Size: 447 34 266 34 488 34 200 Fragments arranged by size: 488 447 266 200 34 34 34 BfrBI ATG'CAT Cuts at: 0 408 1503 Size: 408 1095 BglI GCCn_nnn'nGGC Cuts at: 0 826 1503 Size: 826 677 BglII A'GATC_T Cuts at: 0 845 1503 Size: 845 658 BlpI GC'TnA_GC Cuts at: 0 991 1503 Size: 991 512 Bme1580I G_kGCm'C Cuts at: 0 230 972 1044 1053 1443 1503 Size: 230 742 72 9 390 60 Fragments arranged by size: 742 390 230 72 60 9 BmgBI CAC'GTC Cuts at: 0 157 576 1503 Size: 157 419 927 Fragments arranged by size: 927 419 157 BmrI ACTGGGnnnn_n' Cuts at: 0 697 913 1047 1503 Size: 697 216 134 456 Fragments arranged by size: 697 456 216 134 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 98 499 739 1503 Size: 98 401 240 764 Fragments arranged by size: 764 401 240 98 Bpu10I CC'TnA_GC Cuts at: 0 197 709 1503 Size: 197 512 794 Fragments arranged by size: 794 512 197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 826 1503 Size: 826 677 BsaI GGTCTCn'nnnn_ Cuts at: 0 953 1503 Size: 953 550 BsaAI yAC'GTr Cuts at: 0 429 561 1503 Size: 429 132 942 Fragments arranged by size: 942 429 132 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 55 85 790 820 1503 Size: 55 30 705 30 683 Fragments arranged by size: 705 683 55 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 36 223 676 736 943 1503 Size: 36 187 453 60 207 560 Fragments arranged by size: 560 453 207 187 60 36 BseYI C'CCAG_C Cuts at: 0 37 611 1450 1503 Size: 37 574 839 53 Fragments arranged by size: 839 574 53 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 1168 1503 Size: 619 549 335 Fragments arranged by size: 619 549 335 BsiEI CG_ry'CG Cuts at: 0 622 1503 Size: 622 881 BsiHKAI G_wGCw'C Cuts at: 0 245 972 1016 1044 1241 1503 Size: 245 727 44 28 197 262 Fragments arranged by size: 727 262 245 197 44 28 BsmI GAATG_Cn' Cuts at: 0 248 1503 Size: 248 1255 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1114 1503 Size: 1114 389 Bsp1286I G_dGCh'C Cuts at: 0 230 245 668 972 1016 1044 1053 1214 Size: 230 15 423 304 44 28 9 161 Cuts at: 1214 1241 1443 1503 Size: 27 202 60 Fragments arranged by size: 423 304 230 202 161 60 44 28 27 15 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 494 545 1503 Size: 494 51 958 Fragments arranged by size: 958 494 51 BsrBI CCG'CTC Cuts at: 0 471 546 1503 Size: 471 75 957 Fragments arranged by size: 957 471 75 BsrDI GCAATG_nn' Cuts at: 0 191 1203 1503 Size: 191 1012 300 Fragments arranged by size: 1012 300 191 BsrFI r'CCGG_y Cuts at: 0 594 1503 Size: 594 909 BssSI C'ACGA_G Cuts at: 0 971 1503 Size: 971 532 BstAPI GCAn_nnn'nTGC Cuts at: 0 1048 1503 Size: 1048 455 BstBI TT'CG_AA Cuts at: 0 900 1503 Size: 900 603 BstYI r'GATC_y Cuts at: 0 201 845 1503 Size: 201 644 658 Fragments arranged by size: 658 644 201 BtgI C'CryG_G Cuts at: 0 438 963 1503 Size: 438 525 540 Fragments arranged by size: 540 525 438 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 394 1503 Size: 394 1109 BtsI GCAGTG_nn' Cuts at: 0 1472 1503 Size: 1472 31 DraIII CAC_nnn'GTG Cuts at: 0 1048 1503 Size: 1048 455 DrdI GACnn_nn'nnGTC Cuts at: 0 919 1503 Size: 919 584 EaeI y'GGCC_r Cuts at: 0 218 619 1134 1398 1503 Size: 218 401 515 264 105 Fragments arranged by size: 515 401 264 218 105 EagI C'GGCC_G Cuts at: 0 619 1503 Size: 619 884 EarI CTCTTCn'nnn_ Cuts at: 0 784 790 1503 Size: 784 6 713 Fragments arranged by size: 784 713 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 581 1503 Size: 581 922 EcoICRI GAG'CTC Cuts at: 0 243 1503 Size: 243 1260 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 98 310 499 739 1503 Size: 98 212 189 240 764 Fragments arranged by size: 764 240 212 189 98 EcoNI CCTnn'n_nnAGG Cuts at: 0 1231 1503 Size: 1231 272 EcoO109I rG'GnC_Cy Cuts at: 0 321 1404 1440 1503 Size: 321 1083 36 63 Fragments arranged by size: 1083 321 63 36 FspI TGC'GCA Cuts at: 0 565 1503 Size: 565 938 HaeII r_GCGC'y Cuts at: 0 980 1503 Size: 980 523 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 790 822 1503 Size: 55 32 703 32 681 Fragments arranged by size: 703 681 55 32 32 HincII GTy'rAC Cuts at: 0 675 1466 1503 Size: 675 791 37 Fragments arranged by size: 791 675 37 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 11 157 659 1503 Size: 11 146 502 844 Fragments arranged by size: 844 502 146 11 MscI TGG'CCA Cuts at: 0 220 1136 1400 1503 Size: 220 916 264 103 Fragments arranged by size: 916 264 220 103 MslI CAynn'nnrTG Cuts at: 0 1190 1503 Size: 1190 313 MspA1I CmG'CkG Cuts at: 0 398 449 1437 1503 Size: 398 51 988 66 Fragments arranged by size: 988 398 66 51 NcoI C'CATG_G Cuts at: 0 438 1503 Size: 438 1065 NsiI A_TGCA'T Cuts at: 0 410 1503 Size: 410 1093 NspI r_CATG'y Cuts at: 0 408 462 833 1326 1503 Size: 408 54 371 493 177 Fragments arranged by size: 493 408 371 177 54 PasI CC'CwG_GG Cuts at: 0 161 1503 Size: 161 1342 PflMI CCAn_nnn'nTGG Cuts at: 0 1059 1503 Size: 1059 444 PfoI T'CCnGG_A Cuts at: 0 145 302 1313 1343 1503 Size: 145 157 1011 30 160 Fragments arranged by size: 1011 160 157 145 30 PmlI CAC'GTG Cuts at: 0 429 1503 Size: 429 1074 PpuMI rG'GwC_Cy Cuts at: 0 321 1503 Size: 321 1182 PspOMI G'GGCC_C Cuts at: 0 1439 1503 Size: 1439 64 PspXI vC'TCGA_Gb Cuts at: 0 925 1503 Size: 925 578 PvuII CAG'CTG Cuts at: 0 449 1503 Size: 449 1054 SacI G_AGCT'C Cuts at: 0 245 1503 Size: 245 1258 SexAI A'CCwGG_T Cuts at: 0 731 1031 1503 Size: 731 300 472 Fragments arranged by size: 731 472 300 SfcI C'TryA_G Cuts at: 0 103 1503 Size: 103 1400 SgrAI Cr'CCGG_yG Cuts at: 0 594 1503 Size: 594 909 SmlI C'TyrA_G Cuts at: 0 841 925 1503 Size: 841 84 578 Fragments arranged by size: 841 578 84 SphI G_CATG'C Cuts at: 0 408 833 1503 Size: 408 425 670 Fragments arranged by size: 670 425 408 StyI C'CwwG_G Cuts at: 0 438 809 1503 Size: 438 371 694 Fragments arranged by size: 694 438 371 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 73 932 1503 Size: 73 859 571 Fragments arranged by size: 859 571 73 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 193 225 1503 Size: 193 32 1278 Fragments arranged by size: 1278 193 32 XhoI C'TCGA_G Cuts at: 0 925 1503 Size: 925 578 XmnI GAAnn'nnTTC Cuts at: 0 778 1503 Size: 778 725 Enzymes that do cut: AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PvuII SacI SexAI SfcI SgrAI SmlI SphI StyI TaqII TstI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BbeI BbsI BciVI BclI BmtI BplI BsaBI BsaHI BsaWI BsiWI BspEI BspHI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PpiI PshAI PsiI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XmaI ZraI