################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AlwI 354 GGATC GGATC(4/5) AvrII 222 CCTAGG C^CTAGG BciVI 252 GGATAC GTATCC(6/5) BcnI 35 CCCGG CC^[GC]GG BfaI 223 CTAG C^TAG BlnI 222 CCTAGG C^CTAGG BsgI 169 GTGCAG GTGCAG(16/14) BsmI 28 GCATTC GAATGC(1/-1) BspHI 243 TCATGA T^CATGA BspMI 188 ACCTGC ACCTGC(4/8) BsrDI 54 GCAATG GCAATG(2/0) BstF5I 372 CATCC GGATG(2/0) BstYI 354 GGATCT [GA]^GATC[CT] Cac8I 454 GCAGGC GC[ACGT]^[ACGT]GC DpnII 355 GATC ^GATC DraII 173 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 211 CCATGG C^C[GA][CT]GG EcoNI 9 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 173 AGGACCT [GA]G^G[ACGT]CC[CT] FokI 372 CATCC GGATG(9/13) HapII 36 CCGG C^CGG HinfI 276 GACTC G^A[ACGT]TC HpaII 36 CCGG C^CGG MaeI 223 CTAG C^TAG MboI 355 GATC ^GATC MfeI 476 CAATTG C^AATTG MflI 354 GGATCT [GA]^GATC[CT] MspI 36 CCGG C^CGG MunI 476 CAATTG C^AATTG NciI 35 CCCGG CC^[GC]GG NcoI 211 CCATGG C^CATGG NdeII 355 GATC ^GATC PflMI 565 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 276 GACTC GAGTC(4/5) PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT] RcaI 243 TCATGA T^CATGA Sau3AI 355 GATC ^GATC Van91I 565 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 354 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 3 GTAC GT^AC 593 GTAC AluI 181 AGCT AG^CT 587 AGCT AvaII 174 GGACC G^G[AT]CC 186 GGACC BsrI 79 ACTGG ACTGG(1/-1) 521 CCAGT Cfr13I 174 GGACC G^G[ACGT]CC 186 GGACC EcoRI 116 GAATTC G^AATTC 498 GAATTC EcoT14I 211 CCATGG C^C[AT][AT]GG 222 CCTAGG MboII 130 GAAGA GAAGA(8/7) 409 GAAGA MslI 60 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 445 CATTGTAATG MwoI 210 GCCATGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 608 GCAAAGAAAGC NdeI 296 CATATG CA^TATG 558 CATATG RsaI 3 GTAC GT^AC 593 GTAC Sau96I 174 GGACC G^G[ACGT]CC 186 GGACC SfaNI 109 GATGC GCATC(5/9) 235 GATGC StyI 211 CCATGG C^C[AT][AT]GG 222 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI ApoI BbvI BsaJI BsiYI BslI BssKI BstNI DdeI EcoRII Fnu4HI HphI ItaI MaeIII MnlI MseI MvaI NlaIII ScrFI Tru9I TseI Tsp45I Tsp509I TspRI