(Circular) (Six-base) MAPSORT of: pFN21AE3922.seq Check: 8745 from: 1 to: 7432 pFN21AE3922 7432 bp With 182 enzymes: SgfI * March 28, 2012 11:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5242 278 Size: 53 83 186 936 3706 2468 Fragments arranged by size: 3706 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4775 1937 Size: 2838 4594 Acc65I G'GTAC_C Cuts at: 4753 4753 Size: 7432 AclI AA'CG_TT Cuts at: 1563 5559 5932 1563 Size: 3996 373 3063 Fragments arranged by size: 3996 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2913 5495 6879 7147 1632 Size: 1281 2582 1384 268 1917 Fragments arranged by size: 2582 1917 1384 1281 268 AfeI AGC'GCT Cuts at: 2368 2368 Size: 7432 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7235 AflIII A'CryG_T Cuts at: 1203 2191 2817 3384 6331 7308 1203 Size: 988 626 567 2947 977 1327 Fragments arranged by size: 2947 1327 988 977 626 567 AgeI A'CCGG_T Cuts at: 6263 6263 Size: 7432 AhdI GACnn_n'nnGTC Cuts at: 6161 6161 Size: 7432 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7432 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3765 4655 1184 Size: 2581 890 3961 Fragments arranged by size: 3961 2581 890 AlwNI CAG_nnn'CTG Cuts at: 3242 5175 6747 3242 Size: 1933 1572 3927 Fragments arranged by size: 3927 1933 1572 ApaLI G'TGCA_C Cuts at: 3638 5489 6645 3638 Size: 1851 1156 4425 Fragments arranged by size: 4425 1851 1156 ApoI r'AATT_y Cuts at: 1493 2158 2165 3437 4072 4741 4967 5119 7228 Size: 665 7 1272 635 669 226 152 2109 Cuts at: 7228 1493 Size: 1697 Fragments arranged by size: 2109 1697 1272 669 665 635 226 152 7 AseI AT'TA_AT Cuts at: 160 1032 2105 2747 5986 160 Size: 872 1073 642 3239 1606 Fragments arranged by size: 3239 1606 1073 872 642 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7432 AvaI C'yCGr_G Cuts at: 1870 1943 2075 2761 4757 1870 Size: 73 132 686 1996 4545 Fragments arranged by size: 4545 1996 686 132 73 BamHI G'GATC_C Cuts at: 4762 5129 4762 Size: 367 7065 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4165 4753 6208 618 Size: 325 217 627 2378 588 1455 1842 Fragments arranged by size: 2378 1842 1455 627 588 325 217 BanII G_rGCy'C Cuts at: 1391 4751 1391 Size: 3360 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2808 4473 928 Size: 971 909 1665 3887 Fragments arranged by size: 3887 1665 971 909 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 5623 5657 1903 Size: 34 2120 34 1532 34 3678 Fragments arranged by size: 3678 2120 1532 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5324 6534 5324 Size: 1210 6222 BclI T'GATC_A Cuts at: 1548 2125 3910 1548 Size: 577 1785 5070 Fragments arranged by size: 5070 1785 577 BfrBI ATG'CAT Cuts at: 2183 3553 3845 4632 2183 Size: 1370 292 787 4983 Fragments arranged by size: 4983 1370 787 292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6043 136 Size: 107 122 71 631 605 4371 1525 Fragments arranged by size: 4371 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3942 7427 3942 Size: 3485 3947 BlpI GC'TnA_GC Cuts at: 2924 4849 2924 Size: 1925 5507 Bme1580I G_kGCm'C Cuts at: 1790 3642 5493 6649 1790 Size: 1852 1851 1156 2573 Fragments arranged by size: 2573 1852 1851 1156 BmgBI CAC'GTC Cuts at: 1318 2192 2992 1318 Size: 874 800 5758 Fragments arranged by size: 5758 874 800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3012 3850 5163 Size: 416 53 83 399 24 1595 838 1313 Cuts at: 5163 6121 442 Size: 958 1753 Fragments arranged by size: 1753 1595 1313 958 838 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7432 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4511 Size: 7432 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2855 3219 6092 1239 Size: 1616 364 2873 2579 Fragments arranged by size: 2873 2579 1616 364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4461 4628 4661 5557 6422 6720 4461 Size: 167 33 896 865 298 5173 Fragments arranged by size: 5173 896 865 298 167 33 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6095 882 Size: 617 4596 2219 Fragments arranged by size: 4596 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5223 493 Size: 711 4019 2702 Fragments arranged by size: 4019 2702 711 BsaBI GATnn'nnATC Cuts at: 7426 7426 Size: 7432 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5239 5621 6256 275 Size: 53 83 186 936 3706 382 635 1451 Fragments arranged by size: 3706 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5865 6263 6537 6684 7095 5865 Size: 398 274 147 411 6202 Fragments arranged by size: 6202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2828 2858 3688 3718 1178 Size: 30 165 30 1425 30 830 30 4892 Fragments arranged by size: 4892 1425 830 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2676 2688 4496 1186 Size: 1490 12 1808 4122 Fragments arranged by size: 4122 1808 1490 12 BseYI C'CCAG_C Cuts at: 3935 6635 7131 3935 Size: 2700 496 4236 Fragments arranged by size: 4236 2700 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2477 2564 3339 1163 Size: 573 741 87 775 5256 Fragments arranged by size: 5256 775 741 573 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4882 5643 6671 1540 Size: 275 186 2881 761 1028 2301 Fragments arranged by size: 2881 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3642 5493 5578 6649 3642 Size: 1851 85 1071 4425 Fragments arranged by size: 4425 1851 1071 85 BsmI GAATG_Cn' Cuts at: 2183 2246 3610 4943 5036 2183 Size: 63 1364 1333 93 4579 Fragments arranged by size: 4579 1364 1333 93 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3642 4751 5493 5578 6649 1391 Size: 399 1852 1109 742 85 1071 2174 Fragments arranged by size: 2174 1852 1109 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7095 7095 Size: 7432 BspHI T'CATG_A Cuts at: 5321 7051 5321 Size: 1730 5702 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4787 844 Size: 3943 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5319 1394 Size: 429 3496 3507 Fragments arranged by size: 3507 3496 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2214 4085 4161 5927 6101 65 Size: 1183 966 1871 76 1766 174 1396 Fragments arranged by size: 1871 1766 1396 1183 966 174 76 BsrFI r'CCGG_y Cuts at: 1700 1810 3354 6076 6263 1700 Size: 110 1544 2722 187 2869 Fragments arranged by size: 2869 2722 1544 187 110 BsrGI T'GTAC_A Cuts at: 96 2300 2713 96 Size: 2204 413 4815 Fragments arranged by size: 4815 2204 413 BssHII G'CGCG_C Cuts at: 1925 2057 1925 Size: 132 7300 BssSI C'ACGA_G Cuts at: 5492 6504 5492 Size: 1012 6420 BstAPI GCAn_nnn'nTGC Cuts at: 794 2551 3728 794 Size: 1757 1177 4498 Fragments arranged by size: 4498 1757 1177 BstBI TT'CG_AA Cuts at: 2998 4246 6236 2998 Size: 1248 1990 4194 Fragments arranged by size: 4194 1990 1248 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7432 BstYI r'GATC_y Cuts at: 1972 2646 3942 4432 4762 5129 5514 5531 6983 Size: 674 1296 490 330 367 385 17 1452 Cuts at: 6983 7069 7081 7427 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1296 674 490 385 367 346 330 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2490 3763 513 Size: 552 939 60 426 1273 4182 Fragments arranged by size: 4182 1273 939 552 426 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2030 487 Size: 683 56 804 5889 Fragments arranged by size: 5889 804 683 56 BtsI GCAGTG_nn' Cuts at: 2504 2552 4948 5742 5762 2504 Size: 48 2396 794 20 4174 Fragments arranged by size: 4174 2396 794 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7432 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7397 DraI TTT'AAA Cuts at: 3465 3577 4736 3465 Size: 112 1159 6161 Fragments arranged by size: 6161 1159 112 DraIII CAC_nnn'GTG Cuts at: 4535 4535 Size: 7432 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6439 809 Size: 507 5123 1802 Fragments arranged by size: 5123 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4879 5768 7397 Size: 54 1425 50 275 18 3049 889 1629 Cuts at: 7397 8 Size: 43 Fragments arranged by size: 3049 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4879 1537 Size: 275 3067 4090 Fragments arranged by size: 4090 3067 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2951 4155 5362 1353 Size: 1598 1204 1207 3423 Fragments arranged by size: 3423 1598 1207 1204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2446 6019 6393 6539 387 Size: 1064 138 857 3573 374 146 1280 Fragments arranged by size: 3573 1280 1064 857 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2855 2913 3219 5495 6092 6879 7147 Size: 393 1223 58 306 2276 597 787 268 Cuts at: 7147 1239 Size: 1524 Fragments arranged by size: 2276 1524 1223 787 597 393 306 268 58 EcoO109I rG'GnC_Cy Cuts at: 3239 4525 3239 Size: 1286 6146 EcoRI G'AATT_C Cuts at: 4741 5119 4741 Size: 378 7054 EcoRV GAT'ATC Cuts at: 3664 3664 Size: 7432 FspI TGC'GCA Cuts at: 5938 5938 Size: 7432 HaeII r_GCGC'y Cuts at: 1678 2370 6579 1678 Size: 692 4209 2531 Fragments arranged by size: 4209 2531 692 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 3409 3441 3524 Size: 32 135 32 1256 32 549 32 83 Cuts at: 3524 3556 3800 3817 3832 3849 4031 4063 6073 Size: 32 244 17 15 17 182 32 2010 Cuts at: 6073 6105 6147 6179 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2010 1256 549 244 182 135 83 42 32 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2377 4776 5022 669 Size: 988 281 439 2399 246 3079 Fragments arranged by size: 3079 2399 988 439 281 246 HindIII A'AGCT_T Cuts at: 748 2203 3375 748 Size: 1455 1172 4805 Fragments arranged by size: 4805 1455 1172 HpaI GTT'AAC Cuts at: 5022 5022 Size: 7432 KpnI G_GTAC'C Cuts at: 4757 4757 Size: 7432 MfeI C'AATT_G Cuts at: 5031 5031 Size: 7432 MluI A'CGCG_T Cuts at: 7308 7308 Size: 7432 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6546 6730 1305 Size: 5241 184 2007 Fragments arranged by size: 5241 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5610 Fragments arranged by size: 5610 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2466 4637 5390 5749 5908 518 Size: 648 1300 2171 753 359 159 2042 Fragments arranged by size: 2171 2042 1300 753 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3863 3973 4848 5525 6673 6918 1606 Size: 2257 110 875 677 1148 245 2120 Fragments arranged by size: 2257 2120 1148 875 677 245 110 NcoI C'CATG_G Cuts at: 513 1065 2004 2490 3763 513 Size: 552 939 486 1273 4182 Fragments arranged by size: 4182 1273 939 552 486 NdeI CA'TA_TG Cuts at: 387 4299 387 Size: 3912 3520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7432 NotI GC'GGCC_GC Cuts at: 4879 4879 Size: 7432 NsiI A_TGCA'T Cuts at: 2185 3555 3847 4634 2185 Size: 1370 292 787 4983 Fragments arranged by size: 4983 1370 787 292 NspI r_CATG'y Cuts at: 1132 1229 2465 2513 2821 3388 4369 4632 4642 Size: 97 1236 48 308 567 981 263 10 Cuts at: 4642 4790 6335 1132 Size: 148 1545 2229 Fragments arranged by size: 2229 1545 1236 981 567 308 263 148 97 48 10 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7432 PciI A'CATG_T Cuts at: 2817 3384 6331 2817 Size: 567 2947 3918 Fragments arranged by size: 3918 2947 567 PflMI CCAn_nnn'nTGG Cuts at: 1108 3242 4042 4535 1108 Size: 2134 800 493 4005 Fragments arranged by size: 4005 2134 800 493 PfoI T'CCnGG_A Cuts at: 3235 3409 3235 Size: 174 7258 PmeI GTTT'AAAC Cuts at: 4736 4736 Size: 7432 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5501 5533 7014 7046 5501 Size: 32 1481 32 5887 Fragments arranged by size: 5887 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3239 4525 3239 Size: 1286 6146 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7432 PsiI TTA'TAA Cuts at: 5002 5002 Size: 7432 PstI C_TGCA'G Cuts at: 830 4784 830 Size: 3954 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7432 PvuII CAG'CTG Cuts at: 3863 3973 3863 Size: 110 7322 SalI G'TCGA_C Cuts at: 1936 4774 1936 Size: 2838 4594 SanDI GG'GwC_CC Cuts at: 4525 4525 Size: 7432 SapI GCTCTTCn'nnn_ Cuts at: 2951 2951 Size: 7432 SbfI CC_TGCA'GG Cuts at: 4784 4784 Size: 7432 ScaI AGT'ACT Cuts at: 5680 7392 5680 Size: 1712 5720 SfcI C'TryA_G Cuts at: 826 1045 4780 5915 6596 6787 826 Size: 219 3735 1135 681 191 1471 Fragments arranged by size: 3735 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7432 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7432 SmaI CCC'GGG Cuts at: 1872 4759 1872 Size: 2887 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4476 4643 4676 5536 6437 6699 Size: 197 926 2533 167 33 860 901 262 Cuts at: 6699 820 Size: 1553 Fragments arranged by size: 2533 1553 926 901 860 262 197 167 33 SnaBI TAC'GTA Cuts at: 493 5223 493 Size: 4730 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7432 SphI G_CATG'C Cuts at: 1132 4369 4790 1132 Size: 3237 421 3774 Fragments arranged by size: 3774 3237 421 SspI AAT'ATT Cuts at: 5 52 3982 5356 5 Size: 47 3930 1374 2081 Fragments arranged by size: 3930 2081 1374 47 StuI AGG'CCT Cuts at: 1517 2397 1517 Size: 880 6552 StyI C'CwwG_G Cuts at: 513 1065 2004 2490 3763 4871 513 Size: 552 939 486 1273 1108 3074 Fragments arranged by size: 3074 1273 1108 939 552 486 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4544 5459 5476 5629 5814 6259 325 Size: 1255 2964 915 17 153 185 445 1498 Fragments arranged by size: 2964 1498 1255 915 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2300 2607 2713 Size: 274 80 33 51 1443 323 307 106 Cuts at: 2713 5678 7390 96 Size: 2965 1712 138 Fragments arranged by size: 2965 1712 1443 323 307 274 138 106 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3688 3720 7392 7424 3688 Size: 32 3672 32 3696 Fragments arranged by size: 3696 3672 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7432 XbaI T'CTAG_A Cuts at: 4114 4768 4114 Size: 654 6778 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3863 4443 3863 Size: 580 6852 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7432 XmaI C'CCGG_G Cuts at: 1870 4757 1870 Size: 2887 4545 XmnI GAAnn'nnTTC Cuts at: 3418 5561 3418 Size: 2143 5289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5240 276 Size: 53 83 186 936 3706 2468 Fragments arranged by size: 3706 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI