Restriction map for
pFN21AB0450
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BbeI BcgI BclI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I FauI FbaI FseI
HaeII Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PvuI RsrII SacII SalI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1770 GTCTAC GT^[AC][GT]AC
AccIII 166 TCCGGA T^CCGGA
AclI 1292 AACGTT AA^CGTT
AlwNI 252 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2139 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1604 TGCGCA TGC^GCA
BbrPI 1236 CACGTG CAC^GTG
BbvCI 1918 GCTGAGG CCTCAGC(-5/-2)
BciVI 2115 GTATCC GTATCC(6/5)
BsaWI 166 TCCGGA [AT]^CCGG[AT]
BseAI 166 TCCGGA T^CCGGA
BsmFI 1576 GGGAC GGGAC(10/14)
BspEI 166 TCCGGA T^CCGGA
BspLU11I 1096 ACATGT A^CATGT
BsrBI 847 GAGCGG CCGCTC(-3/-3)
BssSI 844 CACGAG CACGAG(-5/-1)
BstXI 2318 CCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 169 GGATCT [GA]^GATC[CT]
CfoI 1605 GCGC GCG^C
EcoNI 599 CCTACATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1175 GAATTC G^AATTC
EcoT22I 2084 ATGCAT ATGCA^T
FspI 1604 TGCGCA TGC^GCA
HgaI 1955 GCGTC GACGC(5/10)
HhaI 1605 GCGC GCG^C
HinP1I 1605 GCGC G^CGC
HpaI 2191 GTTAAC GTT^AAC
MflI 169 GGATCT [GA]^GATC[CT]
MroI 166 TCCGGA T^CCGGA
MspA1I 2319 CAGCTG C[AC]G^C[GT]G
NdeI 2573 CATATG CA^TATG
NsiI 2084 ATGCAT ATGCA^T
PmaCI 1236 CACGTG CAC^GTG
PmlI 1236 CACGTG CAC^GTG
Psp1406I 1292 AACGTT AA^CGTT
PvuII 2319 CAGCTG CAG^CTG
SapI 2060 GCTCTTC GCTCTTC(1/4)
SbfI 472 CCTGCAGG CCTGCA^GG
SfiI 955 GGCCCTTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1448 GCATGC GCATG^C
Sse8387I 472 CCTGCAGG CCTGCA^GG
StuI 898 AGGCCT AGG^CCT
XcmI 395 CCAATTACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 169 GGATCT [GA]^GATC[CT]
XmnI 2139 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 1096 ACATGT A^C[GA][CT]GT
1237 ACGTGT
AseI 495 ATTAAT AT^TAAT
1014 ATTAAT
AsnI 495 ATTAAT AT^TAAT
1014 ATTAAT
AspHI 12 GAGCTC G[AT]GC[AT]^C
2058 GAGCTC
BalI 374 TGGCCA TGG^CCA
650 TGGCCA
BanII 12 GAGCTC G[GA]GC[CT]^C
2058 GAGCTC
BbsI 285 GAAGAC GAAGAC(2/6)
2248 GAAGAC
BglI 956 GCCCTTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2388 GCCTCACCGGC
BmyI 12 GAGCTC G[AGT]GC[ACT]^C
2058 GAGCTC
BpuAI 285 GAAGAC GAAGAC(2/6)
2248 GAAGAC
BsaAI 1236 CACGTG [CT]AC^GT[GA]
2266 TACGTG
BsaI 1073 GAGACC GGTCTC(1/5)
1418 GAGACC
BsgI 86 CTGCAC GTGCAG(16/14)
1698 CTGCAC
BsiHKAI 12 GAGCTC G[AT]GC[AT]^C
2058 GAGCTC
BsmI 466 GAATGC GAATGC(1/-1)
2086 GCATTC
Bsp1286I 12 GAGCTC G[AGT]GC[ACT]^C
2058 GAGCTC
BspHI 891 TCATGA T^CATGA
2456 TCATGA
BsrFI 609 GCCGGT [GA]^CCGG[CT]
2393 ACCGGC
Cfr10I 609 GCCGGT [GA]^CCGG[CT]
2393 ACCGGC
DraII 123 AGGTCCT [GA]G^G[ACGT]CC[CT]
1576 GGGACCC
EaeI 374 TGGCCA [CT]^GGCC[GA]
650 TGGCCA
EarI 2061 CTCTTC CTCTTC(1/4)
1777 GAAGAG
EcoO109I 123 AGGTCCT [GA]G^G[ACGT]CC[CT]
1576 GGGACCC
EcoRV 1327 GATATC GAT^ATC
1831 GATATC
EcoT14I 1077 CCATGG C^C[AT][AT]GG
1581 CCATGG
Ksp632I 2061 CTCTTC CTCTTC(1/4)
1777 GAAGAG
MluNI 374 TGGCCA TGG^CCA
650 TGGCCA
MscI 374 TGGCCA TGG^CCA
650 TGGCCA
NcoI 1077 CCATGG C^CATGG
1581 CCATGG
PflMI 1077 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1332 CCATTTATTGG
PpuMI 123 AGGTCCT [GA]G^G[AT]CC[CT]
1576 GGGACCC
PshBI 495 ATTAAT AT^TAAT
1014 ATTAAT
PstI 473 CTGCAG CTGCA^G
1805 CTGCAG
RcaI 891 TCATGA T^CATGA
2456 TCATGA
SacI 12 GAGCTC GAGCT^C
2058 GAGCTC
SmlI 1116 CTTGAG C^T[CT][GA]AG
2554 CTCAAG
StyI 1077 CCATGG C^C[AT][AT]GG
1581 CCATGG
Van91I 1077 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1332 CCATTTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BsaJI
BseRI BsiYI BslI BsmAI BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I